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Citations for New England Biolabs :
251 - 300 of 382 citations for Pemetrexed disodium Impurity 30 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... and a volume of cDNA corresponding to 5 ng of the input RNA was subjected to 30 cycles of PCR with the 2 x NEBNext Ultra II Q5 Master Mix (NEB).
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bioRxiv - Synthetic Biology 2022Quote: ... 50 ng of genomic DNA from genome-targeting experiments were PCR amplified with 30-cycles using Q5 High-fidelity DNA Polymerase (NEB) and primers which bind just outside of TS2 or just inside of LE ...
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bioRxiv - Developmental Biology 2022Quote: ... coli and by performing heat shock for 30 seconds at 42°C followed by recovery in SOC outgrowth medium (NEB) for 1 hour ...
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bioRxiv - Molecular Biology 2022Quote: ... digestion at 37°C for 30 minutes and then purified with the Monarch RNA Cleanup Kit (T2050, New England Biolabs) for long RNA (1020 nucleotides to 4187 nucleotides ...
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bioRxiv - Molecular Biology 2020Quote: ... End-repair reaction was cleaned using 1.8X Agencourt AMPure XP beads and eluted in 15 µl of EB that was used for A-tailing reaction in 30 µl of NEBNext dA-Tailing reaction buffer (NEB) with 7.5 units of Klenow fragment exo- (NEB ...
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bioRxiv - Immunology 2021Quote: Similarly for ChIP seq 30 μl ChIP-DNA was processed for library preparation according to ChIP-seq library preparation protocol (NEB) [25] ...
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bioRxiv - Microbiology 2020Quote: ... The efgA coding sequence plus 30 bp at the 5’ end was amplified using primers with 30 nt overlaps to permit Gibson assembly (HiFi DNA Assembly, New England Biolabs) into pAH120 [40] that had been digested with XbaI and NdeI ...
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bioRxiv - Biochemistry 2020Quote: ... Hartmann Analytic) for 30 min at 37°C in 50 µl volumes with 20 units of T4 PNK (New England Biolabs) in the provided reaction buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... the concentration of eluted MBP-mintbody was adjusted to 1 mg/ml and digested with 30 μg/ml Factor Xa (New England Biolabs) for 24 h ...
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bioRxiv - Biochemistry 2021Quote: ... One-way assays were carried out as above except for the exclusion of Gyrase and SmaI was substituted for 30 units Ncol-HF (NEB) or 30 units SacI-HF (NEB) ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μl RT mastermix (2x reaction buffer, 20 mM DTT, 4U murine RNase inhibitor [NEB], 30 U ProtoScript II Reverse Transcriptase [NEB]) were added ...
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bioRxiv - Biochemistry 2021Quote: ... per 1g of wet cell weight and 1 Unit of 30 Units/μL RNase Inhibitor (from human placenta; New England Biolabs (NEB), No ...
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bioRxiv - Synthetic Biology 2021Quote: ... [35] obtained from Benenson lab at ETH Zurich was digested with 30 U of SalI-HF (New England Biolabs (NEB), R3138S ...
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bioRxiv - Microbiology 2021Quote: ... 200 ng ΔVC1807::ErmR transforming DNA was added and reactions were incubated at 30 °C for 10 minutes before the addition of 10 units of DNAse I (NEB) to prevent additional DNA uptake ...
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bioRxiv - Microbiology 2020Quote: ... The pKAS32 vector was restriction digested using SacI and XbaI at 37°C for 1 hour followed by an additional 30 minutes at 37°C with alkaline phosphatase from calf intestine (CIP) (New England Biolabs). Vector backbone and upstream and downstream segments were joined using Gibson assembly (New England Biolabs ...
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bioRxiv - Immunology 2020Quote: ... 70°C for 10s and 72°C for 30s) and 1x 72°C for 5 min) using the Q5 polymerase (New England BioLabs) and cloned into pUC57 using the MEGAWHOP35 method ...
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bioRxiv - Cell Biology 2021Quote: ... according to the manufacturer’s instructions (with an elution in 30 μL sterile filtered MilliQ H2O) and restriction digest with suitable restriction enzymes (New England Biolabs) of about 250 ng plasmid DNA for 3 h ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.52 and 0 nM) in a final volume of 30 μL of amplification mixture including: 1X Phi29 DNA polymerase buffer (NEB), 0.3 μM non-modified PCR forward and reverse primers ...
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bioRxiv - Microbiology 2022Quote: ... double stranded library was amplified using PCR by adding 30 μL of 2X Q5 High Fidelity Master Mix (NEB M0492L), 0.4 μL of 100 μM oDS028 ...
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bioRxiv - Biophysics 2023Quote: ... cells were labeled for 30 min at 37° C with 1 µM Surface BG-Alexa546 (impermeable dye; New England Biolabs) for SNAP in extracellular buffer (EX ...
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bioRxiv - Cell Biology 2023Quote: ... Forward and reverse primers for each sgRNA were annealed by pre-incubation at 37°C for 30 minutes with T4 polynucleotide kinase (PNK; NEB), followed by incubation at 95°C for 5 minutes and then ramp down to 25°C at 5°C/min ...
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bioRxiv - Molecular Biology 2023Quote: ... fixed nuclei resuspended in 171 uL SPBSTM were mixed with 99 uL NTP buffer and 30 uL T7 polymerase mix (New England Biolabs) and incubated in a 1.5 mL LoBind tube (Eppendorf ...
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bioRxiv - Neuroscience 2023Quote: ... with 25–30-bp overlap and used to generate plasmids via the Gibson assembly method65 using T5 exonuclease (New England Biolabs), Phusion DNA polymerase (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: To test the binding affinity of the TBT and TBTG mRNA to the 30S ribosomal subunit we used PURExpress ΔRibosome Kit (NEB) supplemented with 5 μM of 30S ribosomal subunit and 10 μM tRNAfMet in the presence of 1.4 μM of radioactively labelled mRNA (prepared as described above by in vitro translation followed by [32P] labelling as described for the northern blot probe labelling) ...
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bioRxiv - Neuroscience 2022Quote: ... DNA fragments were amplified by PCR using primers (TSINGKE Biological Technology) with 25–30-bp overlap and then assembled using T5 exonuclease (New England Biolabs), Phusion DNA polymerase (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... 25 μg of total RNAs from 12-day-old Arabidopsis leaves were extracted using the Trizol method and incubated with 30 μl of pre-equilibrated oligo d(T)25 magnetic beads (NEB) for 15 minutes at room temperature with continuous rotation ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 nM RNAse-free DNA fragments containing gRNA target sequences and 30 nM Cas9 or Cas12a protein (NEB, Ipswich, MA) were mixed in reaction tubes as per manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... pH = 8 for 16 hours at 56°C followed by 30 minutes at 90°C and an additional digest of 4 units of beta-agarase (M0392S, New England BioLabs) for 1 hour at 65°C to ensure full agarose breakdown ...
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bioRxiv - Microbiology 2024Quote: ... PCRs were done in a 50 µl mix for 30 cycles using Phusion High-Fidelity DNA polymerase (New England Biolabs). Purification of PCR products was routinely performed using the QIAquick PCR Purification Kit (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... 50 ng cDNA was amplified by PCR for 30 cycles using Phusion High Fidelity DNA Polymerase (M0530L; New England Biolabs) using the following primers:
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bioRxiv - Cell Biology 2024Quote: ... Gibson assembly was performed at 50 °C for 30 min using the HiFi DNA Assembly Master Mix (#E2621L, New England Biolabs). Resulting plasmid pAC-AfaI-Cm was transformed into S ...
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bioRxiv - Microbiology 2024Quote: ... the total RNA samples were fragmented using ultrasound (4 pulses of 30 sec at 4°C) followed by a treatment with T4 Polynucleotide Kinase (New England Biolabs). The RNA samples were then split into two halves and one half was subjected to Terminator Exonuclease treatment (+TEX) ...
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bioRxiv - Microbiology 2024Quote: ... and ligated to a puromycin linker primer ([5’Phos]CTCCCGCCCCCCGTCC[SP18][SP18][SP18][SP18][SP18]CC[Puromycin]) using T4 RNA ligase (30 min, 25 °C) (New England Biolabs). First round translation was performed on a 150 µL scale ...
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bioRxiv - Microbiology 2024Quote: ... Strains were typically constructed by PCR amplifying vector and insert fragments with compatible 25-30 bp overhangs and ligating them by Gibson assembly (NEB). For the alt.-3-targeting pCas9 construct ...
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bioRxiv - Cancer Biology 2023Quote: ... Entire DNA sample was amplified in several 30 cycle reactions using Q5 Hot Start High-Fidelity 2X Master Mix (NEB), the amplicons were digested with DpnI (thermo scientific) ...
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bioRxiv - Developmental Biology 2024Quote: ... Purified digested vector and inserts were ligated in a 1:30 vector/insert ratio using the T4 DNA ligase (New England Biolabs) following manufacturer’s protocol ...
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bioRxiv - Pathology 2024Quote: ... Unincorporated primers were removed by Exonuclease digestion for 30 min at 37°C followed 15min inactivation at 80C (New England BioLabs). Samples were diluted 5-fold with DNA suspension buffer (Teknova) ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA was extracted from ∼2 million U2OS cells transfected with siNC or siHELZ and fragmented with restriction enzymes (BsrGI, EcoRI, HindIII, SspI, XbaI, 30 U/each, NEB) following a published protocol 62 ...
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bioRxiv - Plant Biology 2024Quote: ... The RNP complex was obtained by incubating for 30 min at 37°C in 15 µL of EngesCas9 specific buffer (NEB), 6 µg of the commercial EngenCas9 (NEB ...
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bioRxiv - Biochemistry 2023Quote: ... crosslinking of Pex1-AzF was performed after a 10 min 30 deg C incubation in ATP regeneration mix with and without apyrase (NEB#M0398S ...
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bioRxiv - Molecular Biology 2022Quote: ... on-bead decapping and phosphorylation were performed in a 30 μl reaction with 5 units T4 PNK 3’ phosphatase minus (NEB), 2.25 μg GST-Dcp1-Edc1-Dcp2 ...
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bioRxiv - Cancer Biology 2022Quote: A range of 5 to 30 ng of cfDNA was used to generate WMS libraries with NEBNext Enzymatic Methyl-seq Kit (New England Biolabs) per manufacturer instructions.
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bioRxiv - Microbiology 2022Quote: ... following which the beads were washed three times with IP buffer and the bound proteins eluted with 30 μL 3x Sample Loading Buffer (NEB) at 95 °C for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... following which the matrix was washed three times with IP buffer and the bound proteins eluted with 30 μL 3x Sample Loading Buffer (NEB) at 95 °C for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... pooled total RNA was fragmented with ultrasound (4 pulses of each 30 sec at 4°C) and then treated with T4 Polynucleotide kinase (NEB). The RNA of each sample was then divided in half ...
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bioRxiv - Zoology 2023Quote: ... One μl of the resulting cDNA was next amplified by PCR for 30 cycles using either OneTaq DNA Polymerase (New England Biolabs) for RT-PCR experiments ...
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bioRxiv - Biophysics 2022Quote: ... The reaction was initiated with incubating 1 μM Casπ RNP effectors with 20 nM dsDNA target at 37 °C for 30 min and then quenched with loading buffer (Gel Loading Dye Purple 6X, NEB) supplemented with 20 mM EDTA and 25 μg/ml heparin ...
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bioRxiv - Biophysics 2022Quote: ... 1 μM Casπ RNP effectors were incubated with 20 nM target plasmids at 37 °C for 30 min and then quenched with loading buffer (Gel Loading Dye Purple 6X, NEB) supplemented with 20 mM EDTA and 25 μg/ml heparin ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The isolated DNA and the initial infectious plasmid were used as DNA template for polymerase chain reaction (PCR) using 30 cycles and the Q5 High Fidelity DNA Polymerase (New England BioLabs) under the recommended conditions by the manufacturer ...
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bioRxiv - Biochemistry 2023Quote: Genomic DNA was extracted from ∼4 million N2A cells and fragmented with restriction enzymes (BsrGI, EcoRI, HindIII, SspI, XbaI, 30 U/each, NEB) following a published protocol (89) ...