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Citations for New England Biolabs :
101 - 150 of 382 citations for Pemetrexed disodium Impurity 30 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... in the final concentration of 30 ng/ml and ATP (New England Biolabs) in the final concentration of 0.5 mM ...
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bioRxiv - Molecular Biology 2023Quote: ... 30 μl was removed to which 0.6 units Taq hot start polymerase (NEB) was added and distributed to 3 tubes which were immediately subjected to thermal cycling ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli and grown at 30°C to ensure construct stability (New England Biolabs). Plasmid clones were picked and inoculated into liquid LB media for overnight culture followed by miniprep using the PureLink Plasmid MiniPrep Kit (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... RCA was performed at 30°C overnight using Phi29 enzyme mix (NEB, M0269L). fluorescent probes hybridization was done at 2X SCC and 10% formamide buffer mix ...
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bioRxiv - Cell Biology 2023Quote: ... PCR was performed at 30 cycles using Q5 High fidelity DNA Polymerase (NEB) at 0.02 U/µl according to further manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... The eluate was added to ∼ 30 mL of amylose resin (New England Biolabs) and incubated for 30 minutes with circular rotation in 50 mL conical tubes ...
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bioRxiv - Cancer Biology 2023Quote: ... a plug was equilibrated 30 min in 200 μl 1x CutSmart buffer (NEB), digested overnight at 37°C with 1 μl 20 U/μl NotI-HF (NEB R3189S ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 to 40 μg of genomic DNA was digested with HinfI (NEB, R0155) and RsaI (NEB ...
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bioRxiv - Genomics 2024Quote: ... 30 µM of dNTPs (including dCTP/dGTP/dTTP (NEB # N0441S/ N0442S/N0443S respectively) with biotin-14-dATP (ThermoFisher Scientific # 19524016 ...
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bioRxiv - Neuroscience 2021Quote: ... 8 μg of DNA were treated with 30 U of RNase H (NEB, M0297) for 16 h at 37 °C ...
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bioRxiv - Systems Biology 2022Quote: ... 20 μl of ligation mix (30 U/μl T4 DNA ligase (cat. # NEB M0202L), and 1.58 X ligase buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by 30 minute incubation at 37°C with Exonuclease I (New England BioLabs) to digest single stranded
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bioRxiv - Genomics 2021Quote: ... a 30 μl mix with 1.6 μl of T4 DNA ligase (New England Biolabs) was added to each well and placed on a thermocycler (16 °C 60 min followed by 80 °C 30 min for enzyme inactivation) ...
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bioRxiv - Immunology 2020Quote: ... enzymatic digestion was performed 30 min with 1 unit T7 endonuclease (New England Biolabs) at 37 °C ...
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bioRxiv - Genomics 2023Quote: ... and 30% for overnight at 4 °C) with RNase inhibitor [New England Biolabs (NEB), M0314L ...
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bioRxiv - Bioengineering 2023Quote: ... After a 30 cycles PCR reaction with Q5 hot-start high-fidelity polymerase (NEB) following recommended vendor protocol ...
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bioRxiv - Biochemistry 2023Quote: ... 20 μg purified genomic DNA was digested with 30 units EcoR I (NEB, R3101L) and 30 units Hind III (NEB ...
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bioRxiv - Microbiology 2022Quote: ... was combined in a 30 μL reaction with 160 μM SAM (New England Biolabs), 1X Cutsmart buffer (New England Biolabs ...
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bioRxiv - Immunology 2022Quote: ... IgL: GCCGCGTACTTGTTGTTGC) in a 30 μl reaction with Q5 DNA polymerase (New England Biolabs). Cycling conditions were one cycle 98°C/30 s ...
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bioRxiv - Molecular Biology 2024Quote: ... This was followed by a 30-minute digestion at 37°C where RSAP (NEB) was added to the pJM220 digestion mix ...
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bioRxiv - Microbiology 2024Quote: ... RNA was expressed overnight in 30 µL T7 transcription reactions (NEB # E2050S, includes DNAse) with 3 ng of either DNA template ...
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bioRxiv - Plant Biology 2024Quote: ... for 30 minutes and the supernatant mixed with 5 mL Chitin Resin (NEB #S6651), previously washed first with 30 mL of ddH2O and then 20 mL of HEGX buffer ...
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bioRxiv - Cell Biology 2024Quote: ... 25 µL samples were mixed with 30 µL High-Fidelity PCR Master Mix (NEB), 2.5 µL P5 primer (10 µM ...
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bioRxiv - Genetics 2024Quote: ... This insert was cloned into V08 [30] via Golden Gate cloning (New England Biolabs) to generate pSZB1593 ...
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bioRxiv - Microbiology 2022Quote: ... A PCR reaction (30 cycles) was run with Phusion® High-Fidelity DNA polymerase (NEB). The primer pairs im126/im127 and im137/im138 were used to target the reference gene pta (74 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 1 μL cDNA was used in a 30-cycle PCR using Q5 DNA Polymerase (NEB) with primer 8 & 9 or 10 & 11 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 1 μL cDNA was used in a 30-cycle PCR using Taq DNA Polymerase (NEB) with primer 4 & 6 or 5 & 6 ...
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bioRxiv - Genomics 2020Quote: ... After adding 30 µl PCR MM [25 µl 2x LongAmp Taq 2X Master Mix (NEB), 0.2 µl 100 mM forward primer ...
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bioRxiv - Biochemistry 2022Quote: ... Mif2 was treated for 2 h at 30 °C with lambda-phosphatase (New England Biolabs) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2022Quote: ... at 37°C for 30 min and dephosphorylated by 1U of Shrimp Alkaline Phosphatase (NEB) for 30 min at 37°C ...
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bioRxiv - Genomics 2022Quote: ... 30 cycles of endpoint PCR were performed using NEB Standard Taq (New England Biolabs, USA). PCR products (6 μL ...
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bioRxiv - Biophysics 2021Quote: ... 1 mM DTT at pH 7.9) containing 30 units/ml of T4 DNA polymerase (NEB), 100μg/ml single stranded DNA binding protein (gp32 ...
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bioRxiv - Microbiology 2020Quote: ... 30 mM acetyl phosphate or 1 µl Calf Intestinal Alkaline Phosphatase (CIP) (NEB, catalog#: M0290V) was added to the solution ...
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bioRxiv - Cell Biology 2020Quote: ... 30 μg of microsomal proteins were treated with EndoH-Hf (New England Biolabs, Ipswich, MA) for 4 h at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.167 mg/mL MTSEA-biotin) for 30 min and purified with Streptavidin Magnetic Beads (NEB). The biotinylated RNA was eluted with 100 mM DTT and purified with RNA Clean beads (Vazymes) ...
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bioRxiv - Molecular Biology 2022Quote: ... for 30 minutes at 37°C in 1X rCutSmart Buffer (New England Biolabs, cat# B6004S), followed by Trizol extraction ...
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bioRxiv - Genomics 2022Quote: ... and DNA was repaired for 30 minutes with a mixture of T4 DNA ligase (NEB), T4 DNA polymerase I (NEB) ...
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bioRxiv - Neuroscience 2024Quote: ... 30 µl of protein A or G paramagnetic beads (73778S or 9006S; New England Biolabs) were washed and then complexed with primary antibodies or naïve rabbit IgG at 4°C with end-over-end rotation ...
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bioRxiv - Genomics 2023Quote: ... Pre-amplification was performed by addition of 30 μL 2x Q5U Master Mix (NEB M0597S), 0.4 μL 100 μM pre-amplification forward primer and 0.4 μL 100 μM pre-amplification reverse primer (SI Appendix ...
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bioRxiv - Immunology 2023Quote: ... beads were resuspended in 30 µL RNA ligation mix (1X RNA ligase I buffer (NEB), 1 µL RNA ligase I (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... chilled on ice and then combined with 30 U T4 RNA Ligase 1 (NEB M0437M), 40 U Murine RNase Inhibitor (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... coli Poly(A) Polymerase for 30 min following the manufacturer’s protocol (NEB, Ipswich, MA, USA). Polyadenylation levels were assessed with Bioanalyzer chip gel electrophoresis and through evaluation of a size shift increase of ∼500 bp for the 23S and 16S rRNAs ...
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bioRxiv - Molecular Biology 2020Quote: ... were incubated for 12 hours at 30°C with 7.5 Units of Phi29 polymerase (NEB M0269) in Phi29 NEB buffer 1X ...
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bioRxiv - Genomics 2021Quote: ... Total RNA from A549 cells (3 µg in 30 µl) was treated with RppH (NEB M0356) at 30 °C for 1 hr and purified by spin column ...
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bioRxiv - Systems Biology 2021Quote: ... Polylinker libraries were amplified using primers BC_CRX_Nested_F and BC_CRX_R (Supplementary file 6) for 30 cycles (NEB Q5) at an annealing temperature of 67C and then purified with the Monarch PCR kit ...
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bioRxiv - Cell Biology 2022Quote: ... for 30 min at room temperature and sequentially incubated with 1 µM Alexa647-SNAP-dye (NEB) and 1 mM DTT in PBS for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... All ligation reactions were performed at room temperature (RT) for 30 min using T4 ligase (NEB). Bacterial transformation was performed following standard protocols using Stellar competent cells (Takara) ...
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bioRxiv - Molecular Biology 2020Quote: ... The 30 µl of the A-tailing reaction were mixed with Quick Ligase buffer 2X (NEB), 3,000 units of Quick ligase and 5 nM of annealed adaptor (Illumina truncated adaptor ...
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bioRxiv - Cell Biology 2021Quote: ... the second strand was synthesized by using Klenow Fragment (30, _50, exo-; New England Biolabs Inc.) and complement chains of not-so random primers ...
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bioRxiv - Biochemistry 2021Quote: ... semi-quantitative PCR with 30 ng cDNA was performed using LongAmp® Taq DNA Polymerase (NEB) with primers in flanking exons detecting both isoforms MALT1A (146 bp ...