Labshake search
Citations for New England Biolabs :
3901 - 3950 of 7437 citations for rno mir 155 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2022Quote: ... Insertion of PCR product into pET21a was achieved with standard protocols (NEB).
-
bioRxiv - Cell Biology 2023Quote: ... Genomic PCRs were performed using Hot Start Taq 2x Master Mix (NEB). PTGER2 primers that bind to conserved sequences in both human and mouse were used as internal control ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... each with 12-cycles (Phusion High Fidelity PCR kit, New England Biolabs).
-
bioRxiv - Genetics 2022Quote: ... DNA oligos were amplified by PCR using Phusion Polymerase (New England Biolabs). DNA amplicons were ligated to Zero Blunt vector (Thermo ...
-
bioRxiv - Genetics 2022Quote: ... DNA oligos were amplified by PCR using Phusion Polymerase (New England Biolabs). Bbs1 restriction enzyme (New England Biolabs ...
-
bioRxiv - Cell Biology 2023Quote: PCR inserts were amplified using Phusion High-Fidelity DNA polymerase (M0530L, NEB). Amplification primers were designed with a 30 base pair overlap with the linear ends of restriction-digested recipient vectors ...
-
bioRxiv - Genomics 2023Quote: ... The inactivated reactions were purified with the Monarch PCR purification kit (NEB).
-
bioRxiv - Genetics 2023Quote: ... All PCR fragments for cloning were generated using Q5 DNA Polymerase (NEB).
-
bioRxiv - Plant Biology 2023Quote: ... The PCR amplifications were treated with DpnI (New England BioLabs, MA, USA) to remove the template plasmid ...
-
bioRxiv - Neuroscience 2023Quote: ... The PCR product was digested with 1 unit of MwoI (NEB #R0573S) for 2 hrs at 60°C ...
-
bioRxiv - Microbiology 2023Quote: DNA regions for all constructs were PCR amplified using Phusion polymerase (NEB) with primers listed in Table S4 ...
-
bioRxiv - Systems Biology 2023Quote: ... Spin-purified PCR products were digested with BsaI-HF-v2 (R3733; NEB) and the size and integrity of full length and digested PCR products were confirmed on a 4% agarose e-gel (Thermo) ...
-
bioRxiv - Molecular Biology 2023Quote: ... The PCR products were processed according to the Exo-SAP protocol (NEB) and Sanger-sequenced by Azenta Life Services GeneWiz with hisC-ins-f as sequencing primer ...
-
bioRxiv - Cancer Biology 2023Quote: ... All PCR reactions were done with Q5 high-fidelity DNA polymerase (NEB), and PCR products were subsequently purified with DNA clean-up after each PCR and restriction ...
-
bioRxiv - Microbiology 2023Quote: ... Spike chimeras were generated through Gibson assembly of PCR generated fragments (NEB). Primers used for site directed mutagenesis and Gibson assembly are described in table 1 ...
-
bioRxiv - Neuroscience 2023Quote: All PCRs were performed Phusion® High-Fidelity DNA Polymerase (NEB, M0530) and all ligations were performed with T4 DNA ligase (Roche ...
-
bioRxiv - Genetics 2023Quote: ... PCR products (380bp in size) were then digested using AvaI (NEB #R0152L) and separated on agarose gel ...
-
bioRxiv - Genomics 2023Quote: ... The PCR product was digested with BamHI-HF and MluI-HF (NEB), and purified using a Purelink quick PCR purification kit (Invitrogen) ...
-
bioRxiv - Genomics 2023Quote: ... All PCR cloning was performed using Q5 High-Fidelity Polymerase (NEB, M0491) or KOD polymerase (Toyobo ...
-
bioRxiv - Microbiology 2023Quote: ... All PCR reactions were conducted using the Q5 polymerase (New England BioLabs) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... cDNA reactions were purified using the Monarch PCR Purification Kit (NEB #T1030L) and eluted in 10µl elution buffer ...
-
bioRxiv - Cancer Biology 2023Quote: ... sgRNA was PCR amplified using NEB Q5 Hot Start polymerase (NEB, #M0494L). The product was purified using AMPure XP beads (Beckman Coulter ...
-
bioRxiv - Cell Biology 2023Quote: ... were constructed by PCR (Q5 High-Fidelity DNA Polymerase, NEB cat#M0491S) and ligation or Gibson isothermal assembly (NEBuilder HiFi DNA Assembly Master Mix ...
-
bioRxiv - Neuroscience 2023Quote: ... The resulting 5’ biotinylated PCR products were digested with BsaI-HF (NEB) to generate four base-pair overhangs ...
-
bioRxiv - Microbiology 2023Quote: ... The enzyme was removed with the Monarch PCR & DNA Cleanup Kit (NEB) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... Insert and vector PCR fragments were incubated with Gibson Assembly mastermix (NEB) for 1 h and 50°C ...
-
bioRxiv - Microbiology 2023Quote: ... The PCR products were assembled using NEBuilder HiFi DNA Assembly (NEB; E2621) following the suppliers’ specifications ...
-
bioRxiv - Microbiology 2023Quote: ... All PCRs were performed using Q5 High-Fidelity DNA Polymerase (NEB; M0491). The PCR products were assembled using NEBuilder HiFi DNA Assembly (NEB ...
-
bioRxiv - Genetics 2024Quote: ... the left and right homology arms were amplified by pfusion PCR (NEB) using y w genomic DNA as template and primer pairs Ti897/898 and Ti899/900 ...
-
bioRxiv - Microbiology 2024Quote: ... PCR-amplified DNA fragments were assembled by Gibson assembly kit (NEBuilder, NEB) as per manufacturer’s protocol and introduced into DH5α E ...
-
bioRxiv - Biochemistry 2024Quote: ... and digested PCR product by NdeI and XhoI restriction enzymes (NEB, USA) was ligated using T4 DNA ligase (NEB ...
-
bioRxiv - Cell Biology 2024Quote: ... Alice Ting’s plasmid (30) via PCR using Q5 high-fidelity (M0492, NEB). Next ...
-
bioRxiv - Microbiology 2024Quote: ... PCR was performed using Q5 high-fidelity DNA polymerase (New England BioLabs) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... PCR reactions were performed with the Phusion High Fidelity DNA polymerase (NEB) according to the manufacturer’s instruction ...
-
bioRxiv - Cell Biology 2024Quote: ... qRT-PCR was performed using Luna Universal qPCR Master Mix (M3003, NEB) running on the Light Cycler 96 System (Roche) ...
-
bioRxiv - Bioengineering 2024Quote: ... These PCR products were digested with ExoCIP (New England Biolabs, Catalog # E1050L) and used as template for a second ...
-
bioRxiv - Cell Biology 2024Quote: Monarch® PCR & DNA Cleanup Kit (New England Biolabs, cat. no. T1030S)
-
bioRxiv - Microbiology 2024Quote: ... Digested products were cleaned using Monarch® PCR & DNA Cleanup Kit (NEB) and incubated with T4 DNA ligase (NEB ...
-
bioRxiv - Biochemistry 2024Quote: ... 50 µL of PCR product was digested by 1μL of DpnI (NEB) for 2 hours ...
-
bioRxiv - Biochemistry 2024Quote: PCR inserts were amplified with Phusion High-Fidelity DNA Polymerase (M0530L, NEB). Amplification primers were designed with a 30 bp overlap with the linear ends of restriction-digested vectors ...
-
bioRxiv - Biochemistry 2024Quote: All PCRs were performed with the Q5 High-Fidelity DNA Polymerase (NEB) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... Protein-coding sequences for cDNA over-expression or co-immunoprecipitation were cloned into pLenti_CMV_cDNA_Flag_SV40_mCherry vector or pLenti_CMV_cDNA_HA_SV40_EGFP vector by PCR and Gibson assembly (NEB, #E2611L). All plasmids were verified by Sanger sequencing.
-
bioRxiv - Microbiology 2024Quote: ... the ahpC gene was amplified by PCR with Phusion DNA polymerase (NEB) using C ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 25 µL 2X NEBNext high-fidelity PCR master mix (NEB, M0541). To reduce GC/size bias and over amplification of libraries ...
-
bioRxiv - Molecular Biology 2024Quote: ... individual PCR amplicons were produced using Q5 high fidelity polymerase (NEB # M0491S) and isolated from a 0.5X TBE gel by centrifugation of a gel core through an EconoSpin column (Epoch Life Science ...
-
bioRxiv - Genetics 2024Quote: ... PCR reactions were performed with Q5 High-Fidelity DNA Polymerase (NEB, UK). Genomic integration of both constructs were confirmed through PCR and Sanger sequencing ...
-
bioRxiv - Physiology 2024Quote: ... We digested the PCR products with AgeI and NotI (New England Biolabs) and cloned them into the first position of the bicistronic vector pCAG-[first position]-2A-GFP ...
-
bioRxiv - Genetics 2024Quote: ... Quantitative PCR was performed using Luna Universal qPCR Master Mix (NEB, M3003L) and a Roche LightCycler 96 machine (Roche) ...
-
bioRxiv - Neuroscience 2024Quote: ... PCR fragments were ligased by HiFi DNA Assembly Master Mix (NEB, E2621L) and into the pCAG-Puro vector by XhoI enzyme site ...
-
bioRxiv - Microbiology 2024Quote: ... The PCR product was treated with Exonuclease I (NEB, Ipswich, MA, USA) for 15 min at 37 °C ...