Labshake search
Citations for New England Biolabs :
3701 - 3750 of 4333 citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... with the 5’ monophosphates available on the treated viral RNAs using T4 RNA ligase (NEB, Ipswich, MA) for 1 h at 37°C ...
-
bioRxiv - Molecular Biology 2022Quote: ... to digest the bottom strand of dsDNA and truncated the 5’ primer region with USER enzyme (NEB). We added 5 M NaCl 20% v/v to the oligo mix and pre-annealed by heating it at 95°C for 3 minutes ...
-
bioRxiv - Biophysics 2022Quote: ... a third primer ACTB K118TAG phosphorylated at its 5’ end and Taq DNA ligase (New England Biolabs) were added to the reaction mixture ...
-
bioRxiv - Biochemistry 2020Quote: ... 5 μL of plasma was lyophilized and resuspended in 20 μL 1X Rapid PNGaseF buffer (NEB #P0710S) and incubated for 15 minutes at 70°C to denature proteins ...
-
bioRxiv - Cell Biology 2020Quote: ... An 8.9 kb fragment preceding and including exon 5 was amplified by PCR (Phusion DNA Polymerase, NEB) and cloned into a plasmid encoding both a floxed neomycin resistance cassette and a diphtheria toxin negative selection marker ...
-
bioRxiv - Synthetic Biology 2021Quote: ... 10X T4 DNA ligase buffer (5 uL) and T4 DNA ligase (320 units; M0202S, New England Biolabs) and incubating at room temperature for 3 hours and then 65 °C for 10 minutes.
-
bioRxiv - Molecular Biology 2021Quote: ... DNA guides used for cleavage (Supplementary Table S3) were 5’-phosphorylated using T4 PNK (New England Biolabs) except when cleavage with 5’-OH guides ...
-
bioRxiv - Microbiology 2020Quote: ... 1 μL of 5 μM annealed adaptors and 10 μL of Blunt/TA ligase master mix (NEB) was added to each reaction ...
-
bioRxiv - Microbiology 2021Quote: ... and pvhsp70 5’ UTRs were digested using BlpI and SpeI restriction enzymes (New England Biolabs, Ipswich, MA), and agarose gel-purified fragments were subcloned into the plasmid pD-pfcam-Luc also digested with these enzymes ...
-
bioRxiv - Genetics 2020Quote: ... RNA was then degraded by the addition of 5 U RNase H (New England Biolabs, cat. M0297S) and cDNA from every 2 reactions was purified on a single RNA Clean & Concentrator-5 column and eluted in 36 μl NF H2O.
-
bioRxiv - Cell Biology 2022Quote: ... Hybridized oligonucleotides were phosphorylated with 5 U T4 polynucleotide kinase in T4 Ligation buffer (New England Biolabs) for 30 min at 37 °C ...
-
bioRxiv - Developmental Biology 2020Quote: ... Free adaptor was then removed by addition of 1 μL 10 U/μL 5’-Deadenylase (NEB, M0331S). 1 μL 10 U/μL RecJ Exonuclease (Lucigen/Epicentre ...
-
bioRxiv - Cell Biology 2021Quote: ... pStr-KDEL_APV-Dendra2/mCherry/EGFP-ER-5 was constructed by the Gibson assembly (New England BioLabs E2611) of pStr-KDEL_SBP-EGFP-Ecadherin linearized by AscI and XbaI ...
-
bioRxiv - Microbiology 2020Quote: ... This plasmid was then digested with BamHI and NotI and the resulting insert (5’esaAflank_SpecPromoter_kanR_3’esaAflank) was gel extracted (Monarch DNA Gel Extraction Kit, NEB). 100 ng of purified insert was transformed into S ...
-
bioRxiv - Immunology 2021Quote: ... The assembled plasmid pCO2 was transformed into NEB® 5-alpha competent Escherichia coli (High Efficiency) (NEB) according to manufacturer’s instructions (see Supplementary Figure 1 for plasmid maps) ...
-
bioRxiv - Synthetic Biology 2021Quote: ... oPool oligos were phosphorylated at their 5’ ends using T4 Polynucleotide Kinase (New England Biolabs, Ipswich, MA). DNA adapters were then annealed to the phosphorylated oPool oligos by incubating extension ...
-
bioRxiv - Genetics 2022Quote: ... The 5’ adaptor (rArCrArCrUrCrUrUrUrCrCrCrUrArCrArCrGrArCrGrCrUrCrUrUrCrCrGrArUrCrU, IDT) was then ligated to the product using T4 RNA ligase 1 (NEB) at 15 °C for 4 hours ...
-
bioRxiv - Cell Biology 2022Quote: ... 0.2 μM reverse primer and 5 μL Luna® Universal Probe qPCR Master Mix (New England Biolabs)] amplified using the Prism7900HT sequence detection system (Applied Biosystems ...
-
bioRxiv - Genetics 2022Quote: ... The 37nt long 5’ adaptor was ligated to the small RNAs using T4 RNA ligase (M0204S, NEB) overnight at 16°C ...
-
bioRxiv - Genomics 2022Quote: ... was used in a 50 μl-reaction containing 5 μl of NEBuffer r3.1 (10X, NEB, cat# B7203), 26 μl of adapter-ligated DNA and NF water to 50 μl ...
-
bioRxiv - Immunology 2022Quote: ... 5 mM DTT) prior to incubating with 0.5 μCi/μl [γ32P]ATP and T4 PNK (NEB, M0201L) to radiolabel crosslinked RNA ...
-
bioRxiv - Molecular Biology 2022Quote: miRNAs were synthesized by IDT and radio-labeled at the 5’ end with T4 polynucleotide kinase (NEB) and (γ-32P ...
-
bioRxiv - Microbiology 2022Quote: ... followed by adding a 5’ cap using the Vaccina Capping System (cat#M2080S, New England Biolabs Inc.) and a poly-A tail using E.coli Poly(A ...
-
bioRxiv - Microbiology 2022Quote: ... 5 ng of α-HA ChIP or input DNA were end-repaired with T4 DNA polymerase (NEB), Klenow DNA polymerase (NEB) ...
-
bioRxiv - Neuroscience 2022Quote: ... 45 nM pegRNA3-3AGTGA (Appendix Table S1) and 5 µl NEBuilder HiFi DNA Assembly Master Mix (NEB) and incubated at 37°C for 1 h.
-
bioRxiv - Molecular Biology 2022Quote: Approximately 5 μg genomic DNA were digested with 1.5 μl restriction enzymes + 2.5 μl CutSmart buffer (NEB) for 5 hrs at 37°C ...
-
bioRxiv - Bioengineering 2024Quote: ... 5 μL of PCR product from the inner primer amplification was cleaned using 5μL Exonuclease I (NEB) and 2μL rSAP (NEB) ...
-
bioRxiv - Microbiology 2023Quote: ... Plasmids were amplified using NEB 5-alpha F′Iq Competent Escherichia coli (High Efficiency) (NEB, Cat# C2992H), and isolated with PureYield Plasmid Miniprep System (Promega ...
-
bioRxiv - Genomics 2023Quote: ... Reverse transcription reactions (RT +) were then performed with 5 µg of RNA and random hexamers (NEB, #S1330S), using the Superscript III Reverse Transcriptase (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2022Quote: ... 5 μg of DNA was digested with HinfI and RsaI restriction enzymes (New England BioLabs, 50U each) at 37 °C overnight ...
-
bioRxiv - Molecular Biology 2023Quote: ... 5’-phosphorylated and dA-tailed with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645). Samples were ligated to adaptor oligos for multiplex sequencing (NEB ...
-
bioRxiv - Microbiology 2023Quote: ... pETduet-1::5′-flank_SpecPromoter_kanR_3′-flank and were subsequently digested from the plasmid using BamHI and NotI (NEB). The resultant insert (5′-flank_SpecPromoter_kanR_3′-flank ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... containing 12·5 μL of Q5 Hot Start High-Fidelity 2X Master Mix (New England BioLabs, M0494S), 1·1 μL of 10 μM SARS-Cov2-Midnight-1200 primer (either Pool 1 or Pool 2)(17 ...
-
bioRxiv - Genetics 2023Quote: ... and amplified for 5 cycles using NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs; M0541L). After evaluation by real-time PCR ...
-
bioRxiv - Molecular Biology 2023Quote: ... Beads were treated with JET (12.5 nM d-o-p) and/or ScaI-HF (NEB, 5 U) for 15 minutes at 37°C ...
-
bioRxiv - Biochemistry 2023Quote: ... VCE or FCE::T7RNAP fusion and 5 U/μL vaccinia cap 2′-O-methyltransferase (New England Biolabs). Reactions were carried out at indicated temperatures for 1 h ...
-
bioRxiv - Neuroscience 2024Quote: ... 50 µg of protein lysate was treated with 5 µL NeuA (a2-3,6,8,9 Neuraminidase A, P0722L, NEB) or 5 µL of O-Glycosidase (P0733L ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... The assembled plasmid was transformed into NEB 5-alpha (high efficiency) competent cells (New England Biolabs; C2987). After verifying the sequence ...
-
bioRxiv - Bioengineering 2024Quote: ... Initial studies were done in an alternate NEB® 5-alpha strain (New England Biolabs, Catalog # C2987H) where trpA and trpB were replaced with a chloramphenicol resistance cassette ...
-
bioRxiv - Physiology 2024Quote: ... Glycan digestion was achieved with the following enzyme concentrations: 5 μL of α2-3,6,8,9 neuraminidase A (20 U/μL, New England Biolab, NEB) with 1x GlycoBuffer 1 (NEB) ...
-
bioRxiv - Microbiology 2024Quote: ... then combined with 5 μM dNTPs and 2 μL of 10× rCutSmart buffer (NEB, Ipswich, MA, USA) in a total reaction volume of 17 μL ...
-
bioRxiv - Molecular Biology 2024Quote: ... fragments were dephosphorylated by adding 5 μL antarctic phosphatase buffer and 2 μL enzyme (NEB cat#M0289S) and incubating for 1 h at 37°C ...
-
bioRxiv - Genetics 2024Quote: 48 h post-electroporation cells were spun down (300G 5’) and resuspended in DNase I (NEB M0303L) at 37C for 15’ using 10 U of enzyme per ug of plasmid transfected in a final volume of 2 ml of DPBS to eliminate possible carryover plasmid in the exterior of the cells ...
-
bioRxiv - Molecular Biology 2024Quote: ... U2OS cells expressing SNAP-H3.1 or SNAP-H3.3 were incubated with 5 µM SNAP-Cell Block (NEB) for 30 min at 37°C for the quench step ...
-
bioRxiv - Plant Biology 2024Quote: ... as described in the NEB Golden Gate Assembly protocol (5-10 inserts protocol) (NEB, MA, United States). E ...
-
bioRxiv - Molecular Biology 2024Quote: ... the gBlock corresponding to the HIV-1C 5’ LTR was digested with EcoRⅠand BssHⅡ (New England Biolabs), which were also used to digest the pEV731 vector ...
-
bioRxiv - Synthetic Biology 2024Quote: ... UK - protocol version ACDE_9163_v114_revO_29Jun2022) and 5 µL of Quick-NEBNext Quick Ligation Module Quick Ligase (NEB, UK) incubated for 10 minutes at room temperature ...
-
bioRxiv - Biochemistry 2024Quote: ... 5 µl of each sample was mixed with 1 µl of 6X Purple Gel Loading Dye (NEB) and electrophoresed at 110 V for 30 minutes ...
-
bioRxiv - Cell Biology 2024Quote: ... Embryos were injected with 1 mg of gRNA and 5 μ M of Cas9 protein (NEB, 10128356) at 1-cell stage ...
-
bioRxiv - Biochemistry 2024Quote: ... with RBNS RT primer (Supp. Table 5) and PCR amplified via Phusion DNA polymerase (New England Biolabs) with RBNS index primers and RBNS reverse primer (Supp ...