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Citations for New England Biolabs :
3551 - 3600 of 4333 citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... 1 μM Casπ RNP effectors were incubated with 20 nM target plasmids at 37 °C for 30 min and then quenched with loading buffer (Gel Loading Dye Purple 6X, NEB) supplemented with 20 mM EDTA and 25 μg/ml heparin ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... The DNA and DNA Assembly Master Mix were incubated at 50°C for 20 min and then NEB Stable Competent Cells (New England Biolabs) were transformed with the product ...
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bioRxiv - Plant Biology 2023Quote: ... The fully reassembled BdPOX gene was ligated overnight at 16°C with the previously digested (FastDigest Eco31I) and dephosphorylated pGGC000 entry vector (with Antarctic Phosphatase, NEB) to generate pGGC_BdPOX ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated for 30 min at 37°C in appropriate media containing 3µM of cell-permeable SNAP-Cell® 647-SiR (New England BioLabs). Cells were then rinsed twice with fresh media and incubated for 30min at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... 12μg of the extracted genomic DNA was digested for 5h at 37°C with 100U restriction enzyme PvuII-HF (#R3151S, New England Biolabs). The digest was cleaned up using a silica bead DNA gel extraction kit (#K0513 ...
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bioRxiv - Genetics 2023Quote: ... The final cleaned-up Hi-C library was used as input material for Illumina sequencing library prep kit (NEB, E7805) with 6-8 cycles of PCR amplification using KAPA-HiFi (Kapa Biosystems ...
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bioRxiv - Immunology 2023Quote: ... pcDNA3.1-FLAG-ZBTB48 WT was used as template for the generation of the constructs with point mutations in the C-terminal arm using the primers in Table S6 and the Q5 Site-Directed Mutagenesis Kit (NEB). In brief ...
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Nucleolar Pol II interactome reveals TBPL1, PAF1, and Pol I at intergenic rDNA drive rRNA biogenesisbioRxiv - Molecular Biology 2023Quote: ... The isolated DNA was then resuspended in TE buffer and incubated overnight at 37°C with HindIII (New England Biolabs (NEB), Cat# R01045) ...
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bioRxiv - Biochemistry 2023Quote: ... A V5 tag was then appended to the C terminus of the open reading frame using Phusion site-directed mutagenesis (New England Biolabs).
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bioRxiv - Cell Biology 2023Quote: ... Two 100 μl aliquots were incubated overnight at 37°C with and without 1 U Endo H (NEB, Frankfurt, Germany). Then the samples were heated with 100 μl SDS sample buffer for 5 min at 95°C.
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bioRxiv - Cancer Biology 2023Quote: ... lysate volume equivalent to 20-40 µg total protein was first denatured at 100°C for 10 min in 1X glycoprotein denaturation buffer (NEB). Endo H digestion was performed at 37°C for 1h in the presence of 1X GlycoBuffer 3 and 1 µL of Endo H enzyme (NEB) ...
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bioRxiv - Biophysics 2023Quote: ... cells were labeled for 30 min at 37° C with 1 µM Surface BG-Alexa546 (impermeable dye; New England Biolabs) for SNAP in extracellular buffer (EX ...
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bioRxiv - Cell Biology 2023Quote: ... Forward and reverse primers for each sgRNA were annealed by pre-incubation at 37°C for 30 minutes with T4 polynucleotide kinase (PNK; NEB), followed by incubation at 95°C for 5 minutes and then ramp down to 25°C at 5°C/min ...
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bioRxiv - Genetics 2023Quote: ... 300 ng of PCR products were digested at 37° C for 1 hour with 10 units of MfeI-HF and SspI-HF in 1X rCutsmart buffer (New England Biolabs, cat ...
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bioRxiv - Cell Biology 2023Quote: ... The unedited methylated DNA template present in the remaining sample was digested for 1 h at 37°C with DpnI restriction enzyme (New England Biolabs) in CutSmart® Buffer (New England Biolabs) ...
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bioRxiv - Molecular Biology 2023Quote: ... two independent 50 μL reactions with 1 μg of backbone plasmid were digested at 37 °C for 1 hour with MluI-HF and EcoRI-HF (New England Biolabs). After heat-inactivation (65 °C ...
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bioRxiv - Genomics 2023Quote: ... followed by digestion to ribonucleotides by incubation for 2 h at 45 °C with 0.15 U of Nuclease P1 (NEB M0660S) in 10 mM ammonium acetate pH 5 ...
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bioRxiv - Molecular Biology 2023Quote: ... Micro-C libraries were then prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) as described74 and sequenced using an Illumina NovaSeq 6000.
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bioRxiv - Cancer Biology 2023Quote: ... annealing temperature: 49°C) using the EpiMark® Hot Start Taq DNA Polymerase (#M0490L, New England Biolabs, Ipswich, MA, USA).
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bioRxiv - Cell Biology 2023Quote: ... The sequence encoding C-KSR was subcloned into pETM11-SUMO3 via Gibson assembly using Gibson Assembly Cloning Kit (NEB, USA). Sequences encoding N-KSR or C-KSR were subcloned into SfiI sites of pSBbi-pur-H-2Kb (# 111623 ...
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bioRxiv - Molecular Biology 2023Quote: ... The cDNA products were purified by using a Zymo Oligo Clean and Concentrator kit and incubated for 20 min at 37°C in the presence or absence of RNase H or RNase A (New England Biolabs) at a low salt concentration followed by Protease K digestion (as above) ...
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bioRxiv - Microbiology 2024Quote: ... Ligation with gentamycin cassette PCR amplified from the plasmid pACEBac1 (GenevaBiotech) was performed O/N at 16 °C using T4 DNA Ligase (NEB). Competent E ...
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bioRxiv - Neuroscience 2024Quote: ... The homology-directed repair (HDR) plasmid for C-terminal SAX-2::GFP(ju1831) was made using three-fragment Gibson Assembly (New England Biolabs) in which two sax-2 homology arms containing 498 bp upstream of the sax-2 TAA stop codon and 540 bp downstream of the sax-2 TAA stop codon plus the stop codon were amplified from N2 genomic DNA and fused such that they flanked GFP (pDD282) ...
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bioRxiv - Cell Biology 2024Quote: ... Each 1 mL aliquot of microsomes was treated for 10 min at 37°C with 4000 U micrococcal nuclease (New England Biolabs), 2 U RNase-free DNase (Promega) ...
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bioRxiv - Microbiology 2024Quote: ... and held at 72°C for 15 minutes before purification by PCR and DNA cleanup kit (New England Biolabs, T1030S) according to manufacturer instructions with the following modifications ...
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bioRxiv - Microbiology 2024Quote: ... The central part of the dsDNA construct was obtained by digestion of the large C-trap plasmid (see above) with NotI (NEB) produced following published protocols (48) ...
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bioRxiv - Genomics 2024Quote: ... followed by a nested PCR reaction (5 cycles for 70 sec at 72◦C) with individual sample-barcoded i5 and i7 Illumina TruSeq primers (New England Biolabs NEBNext Multiplex Oligos for Illumina ...
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bioRxiv - Genomics 2024Quote: ... with a final extension of 72 °C for 2 minutes using Q5 Hot Start High-Fidelity DNA Polymerase (NEB M0493L). We used 7 cycles for all amplicons ...
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bioRxiv - Cell Biology 2024Quote: ... Digest were quenched by addition of EDTA to 25 mM and proteins were digested by incubation at 37°C for 40 min following addition of SDS and Proteinase K (NEB) to 0.25% and 20 units/ml respectively ...
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bioRxiv - Molecular Biology 2024Quote: ... All others cloning were classically performed by vector and PCR products restriction for 2 hours at 37°C in Cutsmart Buffer (New England Biolabs) with appropriate restrictions enzymes (BamHI ...
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bioRxiv - Microbiology 2024Quote: ... PCR-amplified fragments were digested with NdeI/XbaI and then ligated into NdeI/XbaI-linearized pSCrhaB2 overnight at 16°C using T4 Ligase (New England Biolabs). Modification of pSCrhaB2 to remove ogcA ...
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bioRxiv - Microbiology 2024Quote: ... The samples were incubated for 10 min at 50°C and 1 μL was used as template for a PCR reaction using Phusion DNA Polymerase (M0530S, NEB) as described (ref ...
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bioRxiv - Biophysics 2024Quote: ... We digested the pBS-parS for 2 h at 37°C using NotI-HF or XhoI restriction enzymes (New England Biolabs) and heat-inactivated for 20 min at 80°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were then incubated at 55°C for 10 min prior to addition of 2U RNAseH (New England Biolabs, #M0297S) and further incubation at 55°C for 5 min ...
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bioRxiv - Neuroscience 2024Quote: ... pH 8.0) for 1 h at 37 °C and washes in CSTX buffer (1× CutSmart buffer (New England Biolabs (NEB) B60004) ...
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bioRxiv - Immunology 2024Quote: ... extracted by miniprep and digested overnight at 37°C by ClaI-NotI (for pES4) or ApaI-NotI (for p3A9cbTCR) (New England Biolabs). The digested plasmids were run on a 1% TAE agarose gel (with 1:20000 GelRed nucleic acid gel stain ...
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bioRxiv - Microbiology 2024Quote: ... incubation at 30 °C for 1 h) and removed together with unused Linker 1 in a 30 min incubation with exonuclease RecJ (NEB). The reaction was stopped by heating at 65 °C for 20 min and products purified using a DyEx spin column (Qiagen) ...
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bioRxiv - Cell Biology 2024Quote: ... and inserted between the SgrAI and MfeI sites present at the C-terminal end of mCherry (restriction enzymes provided by NEB). Based on the FL plasmid ...
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bioRxiv - Biochemistry 2024Quote: ... with C-terminal TEV, GFP, 7xHis, and StrepII (Thawani et al., 2020) using the Q5 Site Directed Mutagenesis Kit (NEB). All three constructs were expressed using the Bac-to-Bac Sf9 cell expression system (Invitrogen) ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting oligoduplex was ligated into a target plasmid vector predigested with BsmBI (55 °C for 2 h) using T4 DNA ligase (NEB). Cloning reactions were transformed into chemically competent NEB Turbo E ...
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bioRxiv - Molecular Biology 2024Quote: ... Precipitated cDNAs were stored at -20°C before performing PCR using P5/P7 standard Illumina primers and Phusion HF master mix (NE-M0531L, NEB). Ribosomal RNA contaminants were removed from the final library using Ribocutter which utilises Cas9-guided rRNA depletion (Wilkins and Ule ...
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bioRxiv - Microbiology 2024Quote: ... and 1U RNasin) for 2 hours at 30°C and then incubated with 100 μL of Streptavidin Magnetic Beads (NEB) for 30 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... where samples were heat-shocked for 5 minutes at 95°C to inactivate proteases before they were incubated with 500 units of PNGase F (New England Biolabs) for 4 hours at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... for 15 min at 37°C and immediately purified using the Monarch® RNA Cleanup Kit (New England Biolabs, USA). RNA samples were diluted to the desired working concentration and stored at -80 °C prior to use.
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bioRxiv - Genomics 2024Quote: ... A 100 μL restriction digest of pGH643 was incubated overnight at 37°C (10 μg pGH643, 4.5 μL Esp3I (NEB R0734L), 4.5 μL BlpI (NEB R0585L) ...
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bioRxiv - Genomics 2024Quote: ... with a final extension of 72°C for 2 min using Q5 Hot Start High-Fidelity DNA Polymerase (NEB M0493L). PCR reactions were purified by PCR cleanup beads (UC Berkeley DNA Sequencing Facility ...
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bioRxiv - Biochemistry 2024Quote: ... an aliquot of the dense DNA fraction of the second gradient was desalted and subsequently digested for 1 h at 37°C with single-strand-specific nuclease P1 and RNAseH (both New England Biolabs). Digestion-resistant DNA was purified by phenol/chloroform extraction and ethanol precipitation ...
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bioRxiv - Biochemistry 2024Quote: ... at 37 °C for 20 minutes before being purified using a Monarch RNA purification kit using 2X volume ethanol (New England Biolabs). Concentration determined using a Nanodrop ND-1000 ...
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bioRxiv - Biochemistry 2024Quote: ... Sequences coding 3×HA or 3×HA-GFP tags were added to the C-terminal of the protein using gene-specific PCR and HiFi assembly (NEB). The 3×HA and 3×HA-GFP sequences were cloned from pMXs-3XHA-EGFP-OMP25 plasmid (Addgene 83356).
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bioRxiv - Cell Biology 2024Quote: A human GLP-1R construct with residue Ala316 substituted for Thr (c.946G>A) was generated from the WT sequence by Q5 enzyme site-directed mutagenesis (New England Biolabs) and cloned into the pFastBac vector ...