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Citations for New England Biolabs :
301 - 350 of 355 citations for Solid Phase Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... corresponding to a partial ASY3 DEL allele was excised and purified with a Monarch® DNA Gel Extraction kit (New England Biolabs). Purified PCR products were cloned into pCR™4-TOPO® vector using a TOPO™ TA Cloning™ for Sequencing Kit (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting fragment was then checked by 1% agarose gel and purified with the Monarch® DNA Gel Extraction Kit (New England Biolabs). The purified fragment was precipitated by ethanol precipitation and resuspended in buffer (25 mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... A band at approximately 1 kb was cut out and DNA extracted using the Monarch DNA Gel Extraction Kit (New England Biolabs, US) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... was then gel purified using the Qiagen MinElute Gel Extraction Kit (28604) and subjected to Gibson assembly via the New England Biosciences HiFi DNA Assembly Cloning Kit (NEB: E5520S) to insert the amplified region into the hSyn-eGFP vector following its linearization with BamHI (NEB ...
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bioRxiv - Plant Biology 2021Quote: ... excised and gel extracted using the Monarch DNA Gel Extraction Kit following the manufacturers’ instructions (New England BioLabs; Ipswitch, MA, USA).
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bioRxiv - Microbiology 2021Quote: A549 cells were infected with PR8 at a MOI of 5 and lysates were collected every two hours post infection (hpi) until 8 h RNA extraction was performed with Monarch total RNA Miniprep kit (New England Biolabs GmbH) according to manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR products (donor-5’biotin, donor-unmodified) were gel purified using the Monarch DNA Gel Extraction Kit (New England Biolabs, T1020S) and eluted in 20μl embryo transfer water (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... The digested samples were loaded on an agarose gel to detect and purify fragments ranging from 100-500 bps using the Monarch® DNA Gel Extraction Kit (New England Biolabs). The fragmented gDNA was incubated for 1 hr with 10 μM PhuS in 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Immunology 2020Quote: ... The PCR products were purified from a 1% agarose gel using the Monarch DNA Gel Extraction kit (New England Biolabs, USA) and send for Sanger sequencing provided by Edinburgh Genomics ...
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bioRxiv - Genetics 2020Quote: ... The PCR products were purifified using the FastPure Gel DNA Extraction Mini Kit (Vazyme, DC301-01, Nanjing, China) and then digested by BamHI and HindIII restriction enzymes (New England Biolabs, Inc.). C-terminal EGFP-tagged mouse full-length Pabpn1l was cloned into pcDNA3.1(+ ...
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A genome-scale CRISPR interference guide library enables comprehensive phenotypic profiling in yeastbioRxiv - Genomics 2020Quote: Half of one plasmid extraction was used to prepare a 25 μl digestion in 1x final concentration CutSmart buffer with 20 U XhoI (NEB R0146L) and incubated 1 hour at 37 °C ...
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bioRxiv - Genetics 2021Quote: ... Vector components were purified using the Monarch® DNA Gel Extraction Kit or the Monarch® PCR & DNA Cleanup Kit (NEB) and assemblies were performed using NEBuilder® HiFi DNA Assembly Master Mix (NEB) ...
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bioRxiv - Genetics 2022Quote: ... 500 ng of purified PCR products (D2500, Gel Extraction Kit, OMEGA, USA) were denatured and reannealed in NEB buffer 2 (M0302S, NEB, USA): 95 ℃ ...
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bioRxiv - Microbiology 2022Quote: ... the CmR cassette and the downstream region were bound together by overlap PCR and purified using Monarch DNA Gel extraction kit (New England Biolabs®).
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bioRxiv - Neuroscience 2023Quote: ... at −80 °C prior to RNA extraction and library preparation with NEB Next® Ultra™ RNA Library Prep Kit (NEB) by Novogene ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were harvested by phenol-chloroform extraction and processed according to the NEB Ultra II Directional RNA Sample Prep Kit (New England Biolabs, #E7760S).
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bioRxiv - Immunology 2023Quote: ... DNA was extracted from 10ng of mouse brain tissue as previously described13 using NEB Monarch High molecular weight DNA extraction kit according to manufacturer’s instructions (NEB Cat #T3050).
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bioRxiv - Genomics 2023Quote: ... The linear backbone vector (2,183 base pairs in size) was gel extracted using a gel extraction kit (NEB, Cat. No. T1020S) and assembled with the pegRNA oligos (listed above ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting PCR product was purified by phenol-chloroform extraction and used for in vitro transcription using SP6 RNA-polymerase (NEB, M0207). The resulting sgRNA was purified using the High Pure PCR Cleanup Microkit (Roche ...
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bioRxiv - Microbiology 2023Quote: High molecular weight DNA was extracted from isolate OI25 and its revertants using the Monarch® HMW DNA Extraction Kit for Tissue (New England Biolabs) according to the manufacturer’s protocol with the addition of 50 μg/ml lysozyme (Sigma Aldrich ...
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bioRxiv - Immunology 2023Quote: Total RNA was extracted from 107 hybridoma cells using the MonarchTM total RNA extraction kit (New England BioLabs, Ipswich, MA, USA) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from starter cultures of the three original ancestor isolates and 10 trimethoprim-resistant derivatives using New England Biolabs Monarch Genomic DNA Extraction Kit (New England Biolabs, USA) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... These products were then purified by separation on a 1.0% agarose gel at 100 volts constant for 1 hour and were then purified via the Monarch DNA gel extraction kit (NEB, #T1020L). Purified products were quantified with qubit high sensitivity dsDNA kit (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: High Molecular Weight (HMW) DNA was obtained using the Monarch® HMW DNA extraction kit for Tissue (T3060L, New England Biolabs), as previously described (Low et al ...
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bioRxiv - Genomics 2023Quote: ... was extracted from hTERT RPE-1 dry cell pellets using the Monarch HMW DNA extraction kit for cells & blood (New England Biolabs, NEB T3050L) and for tissue (NEB T3060L) ...
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bioRxiv - Genomics 2024Quote: ... The digest product was run on a 1% agarose gel and the band was excised and purified using a Monarch DNA Gel Extraction Kit (NEB #T1020) following the manufacturer’s recommended protocol ...
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bioRxiv - Microbiology 2024Quote: Approximately 40mg of feces were used for RNA extraction using the Monarch® Total RNA Miniprep Kit (New England Biolabs, NEB) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Approximately 40mg of feces were used for RNA extraction using the Monarch® Total RNA Miniprep Kit (New England Biolabs, NEB) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The RNA extraction and library preparation were performed by Novogene Company Limited with NEB Next® Ultra™ RNA Library Prep Kit (NEB) prior to RNA sequencing (paired-end 150 bp ...
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bioRxiv - Microbiology 2020Quote: ... we separated the PCR products from the parental genomic DNA by gel electrophoresis and purified the PCR fragments using the Monarch DNA Gel Extraction Kit (New England Biolabs, Evry, Fr). The purified fragments were then mixed and fused together using assembly PCR carried out with the Q5 high-fidelity DNA polymerase ...
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bioRxiv - Plant Biology 2021Quote: ... The digestion products were run on agarose gel and purified with Monarch® DNA Gel Extraction Kit (New England Biolabs® Inc.). The asRNA genes were ligated into pLSU-1.1 with T4 DNA Ligase (New England Biolabs® Inc. ...
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bioRxiv - Neuroscience 2022Quote: ... at −80 °C prior to RNA extraction and library preparation (performed by Novogene Company Limited with NEB Next® Ultra™ RNA Library Prep Kit (NEB)) prior to RNA sequencing (paired-end 150 bp ...
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bioRxiv - Plant Biology 2021Quote: ... gene-specific amplicons were diluted ten-fold before barcoding and pools of barcoded amplicons were gel-purified (NEB Monarch DNA gel extraction) before Illumina library preparation and sequencing (Amplicon-EZ service at Genewiz) ...
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bioRxiv - Genomics 2021Quote: ... Total RNA extraction was followed by mRNA capture with the NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs Inc.). For embryo and megagametophyte ...
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bioRxiv - Cell Biology 2022Quote: Amplicons of tagged-Cdep were isolated by gel extraction after PCR amplification using Phusion high fidelity polymerase (New England Biolabs, catalog #E0553L). The amplicons were then cloned into the pJFRC7 5x UAS vector (ref ...
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bioRxiv - Neuroscience 2023Quote: ... at -80 °C prior to RNA extraction and library preparation (performed by Novogene Company Limited with NEB Next® Ultra™ RNA Library Prep Kit (NEB)) prior to RNA sequencing (paired-end 150 bp ...
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bioRxiv - Systems Biology 2022Quote: ... The libraries were then run on a 3% agarose gel and the product was extracted using NEB Monarch DNA Gel Extraction Kit (NEB, Cat# T1020L). Next ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was isolated using a genomic DNA extraction kit (Monarch Genomic DNA Purification Kit, New England Biolabs, Frankfurt am Main, Germany) and the PCR was optimized to yield a single amplicon ...
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bioRxiv - Bioengineering 2023Quote: ... was amplified by PCR from the same vector and its DNA was extracted using the Monarch DNA Gel Extraction Kit (New England Biolabs, Ipswich, MA). GLuc was inserted into the digested backbone through Gibson Assembly Hi-Fi kit (New England Biolabs ...
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bioRxiv - Genomics 2023Quote: ... and the linear backbone vector (5098 base pairs in size) was gel extracted using a gel extraction kit (NEB, Cat. No. T1020S). The second product from the digestion (2878 base pairs ...
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bioRxiv - Genetics 2024Quote: ... HMW DNA was also isolated from six PBMC samples (± 1 x 106 cells) using the Monarch® HMW DNA Extraction Kit for Cells & Blood (NEB, #T3050L) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... DpnI treated PCR products were purified with silica column (FastGene Gel/PCR Extraction Kit, Nippon Genetics Co., Ltd., Tokyo, Japan) and then HiFi assembly reaction (New England Biolabs, Ipswich, MA) was carried out to connect each end of linear DNA products ...
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bioRxiv - Genetics 2023Quote: ... We ran amplified DNA from wildtype and mutant individuals on an agarose gel and purified (Monarch DNA Extraction and Purification kit, new England Biolabs, MA, USA) and cloned the PCR products into JM109 competent cells using pGEM®-T Easy Vector Systems (Promega Corporation ...
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bioRxiv - Genomics 2023Quote: ... was extracted from hTERT RPE-1 dry cell pellets using the Monarch HMW DNA extraction kit for cells & blood (New England Biolabs, NEB T3050L) and for tissue (NEB T3060L) ...
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bioRxiv - Genomics 2024Quote: ... high-molecular-weight (HMW) DNA was extracted from cultured lymphoblasts using the Monarch® HMW DNA Extraction Kit for Cells & Blood (New England Biolabs, T3050L). At all steps ...
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bioRxiv - Molecular Biology 2021Quote: Extraction of total RNA from HCT116 +TetOsTIR cells was performed by using Monarch® Total RNA Miniprep Kit (New England Biolabs, Inc, #T2010S). cDNA library construction using TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human (Illumina #RS-122-2201 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and single bands of the expected size were excised and purified with a Monarch® DNA Gel Extraction kit (New England Biolabs, MA, USA). Purified PCR products were cloned into pCR-XL-TOPO™ vector using a TOPO™ XL PCR Cloning Kit following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The digested products were separated by 0.8% agarose gel electrophoresis and purified using the Monarch® DNA Gel Extraction Kit (New England Biolabs, Ipswich, MA, USA). The purified products were ligated using T4 DNA ligase (New England Biolabs ...
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bioRxiv - Genomics 2021Quote: ... gDNA was extracted from 1 ml of overnight culture with the kit Monarch HMW DNA Extraction kit (T#3060 New England Biolabs; Ipswich, MA, USA) following the manufacturer instructions for High Molecular Weight DNA extraction from gram-negative bacteria ...
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bioRxiv - Neuroscience 2022Quote: ... nuclei of 106 cells plated on a 35 mm dish were isolated by addition of nuclei extraction buffer (NEB, 20 mM Hepes (KOH) pH 7.9 ...