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Citations for New England Biolabs :
251 - 300 of 355 citations for Solid Phase Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... One piece of brain tissue (∼300 mg) was placed in 3 ml of ice-cold nuclei extraction buffer (NEB) [0.32 M sucrose ...
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bioRxiv - Cancer Biology 2021Quote: ... The PCR products were resolved by agarose gel electrophoresis and purified using the Monarch Gel Extraction kit (New England Biolabs). Purified PCR products were Sanger sequenced by Genewiz using the BPNT1_F_Seq primer ...
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bioRxiv - Genetics 2021Quote: ... high molecular weight genomic (HMW) gDNA was extracted from homozygous 1619P15 pupae using the Monarch HMW DNA extraction kit for tissue (NEB). The Cas9 Sequencing Kit (Oxford Nanopore Technology ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and single bands of the expected size were excised and purified with a Monarch DNA Gel Extraction kit (New England Biolabs). Libraries were constructed using 1ng of input DNA in a Low Input ...
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bioRxiv - Neuroscience 2021Quote: ... Resulting PCR products were gel purified using Roti-Prep Gel Extraction (Carl Roth) and assembled with Bbsl-linearized pCFD6 backbone using NEBuilder HiFi DNA Assembly Master Mix (NEB) or In-Fusion HD Enzyme Premix (Takara Bio) ...
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bioRxiv - Immunology 2021Quote: ... one as Cytosolic Extraction Buffer (CEB) (HEPES 10 mM; KCl 60 mM; EDTA 1 mM; NP40 1%) and another one as Nuclear Extraction Buffer (NEB) (HEPES 20 mM ...
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bioRxiv - Plant Biology 2020Quote: ... The resulting fragments were gel-purified with the QIAquick Gel Extraction Kit and ligated using T4 DNA Ligase (New England Biolabs) into dephosphorylated pBlueScript KS+ that had also been digested with NotI and gel-purified to make pBS-SS-TOM ...
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bioRxiv - Microbiology 2021Quote: ... or 230 nucleotides (rLLOV-ZsG-IRins-MARVUTR+tr) were excised and purified via Monarch DNA Gel Extraction Kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting fragments were gel-purified with the QIAquick Gel Extraction Kit and ligated using T4 DNA Ligase (New England Biolabs) into dephosphorylated pBlueScript KS+ ...
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bioRxiv - Plant Biology 2021Quote: ... IPT3 promoter DNA sequence was extracted and purified from the gel using Monarch DNA Gel Extraction Kit (New England Biolabs) and Sanger sequenced at Genewiz (South Plainfield ...
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bioRxiv - Plant Biology 2021Quote: ... PCR product of the expected size was purified from the agarose gel after electrophoresis using the Monarch DNA Gel Extraction Kit (NEB), and cloned into the pDONR207 vector (Invitrogen ...
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bioRxiv - Genomics 2021Quote: ... High–molecular weight DNA for Nanopore sequencing was obtained by a customized cetyltrimethylammonium bromide (CTAB) extraction procedure (Schwessinger, 2019; Schwessinger & Rathjen, 2017) with the modification of using RNase A (T3018, NEB) instead of RNase T1 ...
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bioRxiv - Molecular Biology 2022Quote: ... the sRNA fraction (∼15- 35nt) was isolated by gel extraction and RNA species bearing 5’-OH were monophosphorylated by T4 polynucletide kinase (NEB). This was followed by treatment by a terminator exonuclease (Epicentre) ...
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bioRxiv - Genetics 2022Quote: ... This pooled mixture was run on a 2% agarose gel and we extracted and purified DNA in the 400 bp to 600 bp region using the Monarch Gel Extraction Kit (NEB) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2022Quote: ... and extracted and purified DNA in the 400 bp to 600 bp region using the Monarch Gel Extraction Kit (NEB) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... One piece of mouse frontal cortex tissue (6-10 mg) was placed in 3 ml of ice-cold nuclei extraction buffer (NEB) [0.32 M sucrose ...
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bioRxiv - Microbiology 2021Quote: ... Fragments between ∼1 kb and ∼8 kb were size selected by excision with a clean razor blade and DNA was purified from the agarose by a silica column-based gel extraction kit (New England Biolabs, cat#T1020S ...
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bioRxiv - Molecular Biology 2019Quote: ... Size selection was performed by running samples on a 1.8% LMT agarose gel at 80 V for 40 minutes at room temperature and gel extracting the mononucleosome band with a Monarch Gel Extraction Kit (T1020S, New England Biolabs). Note that of the samples digested with 10 ...
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bioRxiv - Cell Biology 2019Quote: ... The YAP-T2A-mCherry sequence was obtained by gel extraction (Cat. # 740609, Machery-Nagel) and inserted into the pCAGeGFP using ligation reaction (Cat. # M0202, New England Biolabs) to produce the pCAG-YAP expression vector ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We treated DNA extractions with 2 μl of RNAase A (ThermoScientific) and purified them with Monarch PCR & DNA Cleanup Kit (New England Biolabs). We assessed DNA quantity and quality with Qubit 3.0 (Thermofisher) ...
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bioRxiv - Synthetic Biology 2019Quote: ... The amplicon corresponding to a genomic fragment of 1240 bp was run on a gel and the DNA was excised and purified with Monarch DNA Gel Extraction Kit (NEB). DNA concentration and absorbance ratio were measured with a NanoDrop Spectrophotometer (ThermoScientific) ...
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bioRxiv - Biophysics 2019Quote: ... final products were separated from non-ligated fragments by electrophoresis using a 0.8-1.5% (m/V) agarose gel and extracted from the gel with the Monarch® DNA Gel Extraction Kit (NEB).
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bioRxiv - Genetics 2021Quote: ... Barcodes were purified by phenol-chloroform extraction and isopropanol precipitation after which they were digested overnight with 80 units of NheI (#R0131S; NEB) and purified using magnetic bead purification (#CPCR-0050 ...
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bioRxiv - Microbiology 2020Quote: ... An aliquot of each PCR reactions was checked by gel electrophoresis for single amplicons before purifying the remaining reaction using Monarch DNA Gel Extraction Kit (NEB), per manufacturer instructions ...
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bioRxiv - Microbiology 2020Quote: ... electrophoresed and the 200-bp bands were excised from agarose gel slice using Monarch DNA Gel extraction Kit (NEB, #T1020S). Then ...
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bioRxiv - Microbiology 2020Quote: BsaI digested fragment were gel purified by using Gel Extraction Kit (OMEGA) and ligated with T4 ligase (New England Biolabs) at room temperature for 1h ...
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bioRxiv - Immunology 2021Quote: ... Samples were recovered by phenol/CHCl3/isoamyl alcohol extraction twice and purified as per kit instruction using Monarch PCR and DNA cleanup Kit (New England BioLabs). DNA was suspended in 200 ul pre-warm (55°C ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions between 100–400 nt were cut and DNA was extracted from the gel by Monarch DNA Gel extraction Kit (NEB). Multiplexed samples were submitted to the IMG Genomics and Bioinformatics facility for library synthesis using the NextSeq® 500/550 High Output Kit v2 ...
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bioRxiv - Biophysics 2022Quote: ... the construct was digested with EcoRV to eliminate ungapped fragments10 analyzed on a 1 % agarose gel and purified (Monarch DNA gel extraction kit, New England BioLabs). The final dsDNA constructs were 8’750 base pairs long with biotin attached on one end ...
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bioRxiv - Neuroscience 2022Quote: ... was amplified by PCR from the same vector and its DNA was extracted using the Monarch DNA Gel Extraction Kit (New England Biolabs). DNA segments for Fc regions ...
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bioRxiv - Molecular Biology 2022Quote: ... An equal number of nuclei in NIB was divided into four tubes and extracted with an equal volume of nuclei extraction buffer (NEB) containing 10 mM Tris-HCl (pH 7.5) ...
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Dual targeting factors are required for LXG toxin export by the bacterial type VIIb secretion systembioRxiv - Microbiology 2022Quote: ... Plasmids were then digested with BamHI and NotI and the deletion fragment was gel extracted (Monarch DNA gel extraction kit, NEB). 100ng of purified deletion fragment was then added to competent S ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA from the whole nuclei or nuclear condensate fractions were extracted using genomic DNA extraction kit (New England Biolabs). DNA-seq library was prepared from 50 ng of extracted genomic DNA using Illumina Nextera DNA Library Prep kit ...
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bioRxiv - Developmental Biology 2023Quote: ... The corresponding bands were cut out and pooled all in one tube followed by purification using the Monarch DNA Gel extraction Kit (T1020; NEB) and subsequently repurified using the DNA cleanup kit (T1030 ...
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bioRxiv - Molecular Biology 2023Quote: ... The library was resolved on a 1% agarose gel and the smear between 300-600 bp was extracted using Monarch DNA Gel Extraction Kit (New England Biolabs).
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bioRxiv - Genomics 2022Quote: ... crushed tissue from the head and legs of a single larvae was used for DNA extraction with Monarch Genomic DNA Purification Kits (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and size selected by running samples on a 1.8% LMT agarose gel at 120 V for 25 minutes at 4°C and gel extracting the mononucleosome band with a Monarch Gel Extraction Kit (T1020S, New England Biolabs). Purified samples were carried forward for qPCR or library preparation ...
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bioRxiv - Neuroscience 2023Quote: RNA was extracted using the QIAzol and the NEB Monarch RNA extraction kit and ribosomal RNA was depleted using the NEBNext rRNA depletion kit (NEB) ...
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bioRxiv - Cell Biology 2023Quote: ... high-molecular weight genomic DNA (gDNA) was isolated by phenol-chloroform extraction and digested with 50 U AluI (NEB, R0137L) and 50 U MboI (NEB ...
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bioRxiv - Genetics 2024Quote: ... was extracted via the UHMW DNA extraction protocol of the Nanobind CBB Big DNA kit (Circulomics) or via the Monarch HMW DNA Extraction Kit for Cells & Blood (New England Biolabs) and quantified using Qubit dsDNA Broad Range kit (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... whereas PCR fragments from the agarose gel were purified using the High-Yield PCR Cleanup and Gel Extraction Kit (New England BioLabs). Site-specific mutagenesis of efp was carried out with the Q5® Site-Directed Mutagenesis Kit (New England BioLabs ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were then mixed with 350 μl 1× RNA protection reagent and RNA was purified using a Monarch total RNA extraction kit (NEB) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were run on a 1% agarose gel and DNA was extracted using Monarch DNA Gel Extraction Kit (New England Biolabs). DNA was sequenced by Sanger sequencing using either the forward or reverse primer ...
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bioRxiv - Synthetic Biology 2024Quote: ... pSpy0K6 and p7INT.1 was extracted using the Monarch® HMW DNA Extraction Kit for Tissue (New England Biolabs®) according to the manufacturers protocol for Gram-positive bacteria (low input ...
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bioRxiv - Plant Biology 2024Quote: ... 75 roots were ground to a powder using a cooled pestle and mortar and homogenized in 20 ml Nuclei Extraction buffer (NEB) (20 mM MOPS (pH 7) ...
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bioRxiv - Biochemistry 2024Quote: ... The PCR product was purified by phenol chloroform extraction and RNA was synthesized using the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB). 1 ug of starting linear DNA template was used and transcription was performed for 2hrs at 37°C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we multiplexed the different DNA extractions using the NebNext Ultra II FS Library Prep Kit (#E7805, #E6440 and #E6177; NEB). Briefly ...
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bioRxiv - Cell Biology 2024Quote: THP-1 monocytes were harvested and lysed for RNA extraction using the Monarch Total RNA Miniprep kit (New England Biolabs), according to the manufacturer protocol ...
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bioRxiv - Genomics 2024Quote: Between 2 and 4 million cells were harvested by centrifugation and high molecular weight (HMW) genomic DNA was extracted using the Monarch High Molecular Weight DNA Extraction kit (NEB) and agitation speeds of 1,500 rpm.
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bioRxiv - Molecular Biology 2024Quote: High molecular weight genomic DNA was extracted from homozygous hDMDTg and hDMDTgSc mice liver using the Monarch HMW DNA Extraction Kit for Tissue (NEB). Oxford Nanopore Technologies Promethion genome sequencing was performed as a service by Novogene ...