Labshake search
Citations for New England Biolabs :
201 - 250 of 355 citations for Solid Phase Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2023Quote: ... We extracted high-molecular weight DNA with the Monarch HMW Extraction Kit for tissue (New England BioLabs) and followed manufacturer recommendations ...
-
bioRxiv - Biochemistry 2023Quote: ... extraction of genomic DNA with the Monarch® Genomic DNA Purification Kit (New England Biolabs, Ipswich, USA), Gibson assemblies with the NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs ...
-
bioRxiv - Plant Biology 2023Quote: ... Total RNA was extracted from whole coleoptiles at 7dpi using the NEB Total RNA extraction kit (NEB) and following kit instructions ...
-
bioRxiv - Microbiology 2023Quote: ... Genomic DNA was extracted using the Monarch genomic DNA extraction and purification kit (New England Biolabs Inc.). Region of Cori and ter were quantitatively amplified using the Sybr green-based Luna Universal One-step RT-qPCR kit (New England Biolabs Inc.) ...
-
Regulation of transcription patterns, poly-ADP-ribose, and RNA-DNA hybrids by the ATM protein kinasebioRxiv - Molecular Biology 2023Quote: Total RNA extraction was performed using NEB Monarch Total RNA Miniprep Kit (New England Biolabs Cat #T2010S). RNAseq library prep was performed using NEBNext Ultra II RNA Library Prep for Illumina (NEB Cat# E7770S) ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Fully homogenized tissue was then used for DNA purification using the Monarch gDNA extraction kit (NEB biotechnologies). DNA extract quality was assessed for quality (260:230 nm and 260:280 nm wavelengths ...
-
bioRxiv - Molecular Biology 2024Quote: ... 3 uL RNase A and 1 uL ProtK all from the Monarch gDNA extraction kit (NEB, T3010). gDNA was extracted following the extraction kit’s protocol with the exception that 600 uL of gDNA binding buffer were added to 400 uL quenched reaction and added to the extraction column on two spins of 2 minutes at 1000g ...
-
bioRxiv - Microbiology 2020Quote: ... PCR protocols entailed an initial phase of 95° C for 3 min using Hot Start Taq polymerase (New England BioLabs Inc., Ipswich, MA, USA), followed by 35 cycles at 50°C and 52°C annealing temperatures for 16S and ITS amplicons respectively ...
-
bioRxiv - Microbiology 2021Quote: ... The final libraries were purified on 2% preparative agarose gel by Monarch DNA Gel Extraction kit (NEB, T1020L). The concentration of each library was measured by performing quantitative PCR (qPCR ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Products from the first round PCR were then purified using Monarch DNA Gel Extraction Kit (New England Biolabs) and used as the templates for the second round insert PCR ...
-
bioRxiv - Biophysics 2022Quote: ... Each PCR product was then run on an agarose gel and purified using a gel extraction protocol (NEB). The purified PCR product concentrations were measured using the Picogreen assay (Thermo Fisher) ...
-
bioRxiv - Plant Biology 2022Quote: ... DNA was subsequently extracted from T0 plants using Monarch DNA extraction kits (New England Biolabs, Ipswich, MA, USA) and sequence confirmed (CHU de Québec-Université Laval ...
-
bioRxiv - Microbiology 2022Quote: ... Resultant fragments were then gel-purified using the Monarch DNA Gel extraction kit (New England Biolabs, MA, USA) and ligated with the T4 DNA ligase (New England Biolabs ...
-
ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... as was the pCDH vector before purification again with Monarch® DNA Gel Extraction Kit (New England Biolabs). The ACE2 fragment was ligated with pCDH vector using T4 DNA Ligase (New England Biolabs ...
-
bioRxiv - Genetics 2022Quote: ... PCR-products were gel purified after agarose gel electrophoresis with Monarch DNA Gel Extraction Kit (New England Biolabs) and submitted for sequencing ...
-
bioRxiv - Molecular Biology 2020Quote: ... Gel extraction was done using Gel breaker tubes (IST Engineering Inc) in DNA Gel Elution Buffer (NEB-kit). Disintegrated gels were incubated at 37°C for 1h on a shaker ...
-
bioRxiv - Genomics 2022Quote: ... The correct size band was cut and purified with the Monarch Gel Extraction Kit (New England BioLabs T1020L). The insert single-stranded DNA was diluted to 1 μM with H2O ...
-
bioRxiv - Neuroscience 2022Quote: ... and a single band of the expected size was purified using Monarch Gel Extraction Kit (New England Biolabs). Purified amplicons from 6 Cas9-only control injected larva and from 24 gRNA-injected (and imaged ...
-
bioRxiv - Molecular Biology 2022Quote: Genomic DNA was extracted using the Monarch® HMW DNA Extraction Kit (New England Biolabs, T3050S and T3060S) using manufacture’s protocol for iPSC samples ...
-
bioRxiv - Microbiology 2023Quote: ... PCR reactions were undertaken using sporocarp extractions using the PHusion High-Fidelity PCR Master Mix (New England Biolabs). Libraries were generated using the TruSeq DNA PCR-Free Prep Kit (Illumina ...
-
bioRxiv - Genetics 2023Quote: ... PCR products were purified after agarose gel electrophoresis using the Monarch DNA Gel Extraction Kit (New England Biolabs) and submitted for Sanger sequencing to Eurofins Genomics ...
-
bioRxiv - Genomics 2023Quote: ... cahirinus animal using a Monarch HMW DNA Extraction Kit for Cells & Blood (T3050, New England Biolabs, Ipswich MA) following the manufacturer’s recommended protocol ...
-
bioRxiv - Plant Biology 2023Quote: ... Amplified PCR products with appropriate expected sizes were purified with the Monarch® DNA Gel Extraction Kit (NEB). Purified PCR products were cloned into the pMiniT 2.0 vector (NEB ...
-
A gene desert required for regulatory control of pleiotropic Shox2 expression and embryonic survivalbioRxiv - Developmental Biology 2023Quote: ... After phenol/chloroform extraction and ethanol precipitation the samples were digested overnight with the secondary enzyme DpnII (NEB) followed by phenol/chloroform extraction ...
-
bioRxiv - Cell Biology 2023Quote: ... RNA extraction for other experiments was performed using the Monarch Total RNA Miniprep Kit (T2010S, New England Biolabs) according to the provided protocol.
-
bioRxiv - Genomics 2024Quote: Hadrurus arizonensis DNA was extracted using the MonarchⓇHigh Molecular Weight (HMW) DNA Extraction Kit: Tissue Protocol (NEB, T3060). Changes made to the protocol include ...
-
bioRxiv - Developmental Biology 2024Quote: ... the specific product was purified from the agarose gel using the Monarch DNA Gel Extraction Kit (NEB, T1020S). Each reverse primer contains the T3 RNA Polymerase minimal promoter sequence (shown in bold and underlined in Table 1) ...
-
bioRxiv - Synthetic Biology 2021Quote: ... total DNA from one clone was isolated using the Monarch Kit for HMW DNA Extraction from Bacteria (NEB#T3060) and confirmed by Nanopore sequencing.
-
bioRxiv - Molecular Biology 2019Quote: ... and then permeabilized in ice cold extraction buffer (CSK, 0.1% TX-100 and 10 mM Ribonucleoside Vanadyl Complex (NEB)) for 5 minutes ...
-
bioRxiv - Genomics 2021Quote: ... and were excised between 300 bp - 900 bp and cleaned using the Monarch DNA Gel Extraction Kit (NEB, T1020). The libraries were quantified and qualified using an Agilent 2100 Bioanalyzer with a High Sensitivity DNA analysis kit (Agilent ...
-
bioRxiv - Molecular Biology 2021Quote: ... Half of each forebrain was immersed in 4.8 ml nuclei extraction buffer (NEB [Sucrose 0.32M, CaCl 5mM, Mg(Ac).4H20 3mM ...
-
bioRxiv - Cancer Biology 2022Quote: ... The PCR product was then gel purified with NEB Monarch® DNA Gel Extraction Kit (NEB, Cat No. T1020). Alongside pLentiCRISPRv2 (Addgene ...
-
bioRxiv - Molecular Biology 2022Quote: ... The pellets containing intact nuclei were vortexed at maximum setting for 15 sec with the nuclear extraction buffer (NEB) and then incubated at 4°C for 30 min with gentle mixing ...
-
bioRxiv - Cell Biology 2022Quote: ... for 2 hours at 37°C and purified using Monarch® DNA Gel Extraction Kit (T1020S, New England Biolabs) before digestion-ligation with the gRNA-pScaffold-H1 using FastDigest Esp3I and T7 Ligase (M0318L ...
-
bioRxiv - Microbiology 2019Quote: ... Amplified fragments of ∼4.5 kb corresponding to gokushovirus prophages were purified from agarose gels using the Monarch DNA Gel Extraction Kit (NEB) and eluted in 20 µl ddH2O ...
-
bioRxiv - Developmental Biology 2019Quote: ... after RNA extraction mRNA was enriched through polyA selection with the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) according to manufacturer’s instructions ...
-
bioRxiv - Genetics 2020Quote: ... DNA that migrated between the 400 and 600 bp was extracted using Monarch DNA Gel Extraction Kit (NEB T1020L). The pooled library DNA concentration was determined using Qubit dsDNA BR Assay Kit (ThermoFisher Scientist Q32853) ...
-
bioRxiv - Biochemistry 2021Quote: ... Both fragments were run on a 1% agarose gel and purified using a Monarch DNA Gel Extraction kit (NEB). The fragments were then joined using the NEBuilder HiFi DNA Assembly protocol (NEB).
-
bioRxiv - Microbiology 2021Quote: BsaI digested fragments were gel purified using Gel Extraction Kit (OMEGA) and ligated with T4 ligase (New England Biolabs) at room temperature for 1 h ...
-
bioRxiv - Biochemistry 2022Quote: ... The product bend was cut and DNA was extracted from the gel by Monarch DNA Gel extraction Kit (NEB). 32P-GTP labelled U1 RNA was prepared by in vitro transcription as described previously with addition of 0.5 μL of α-32P-GTP (3.3 μM ...
-
bioRxiv - Microbiology 2022Quote: ... Genomic DNA of the gram-negative bacteria was extracted using the Monarch DNA Gel Extraction Kit (New England Biolabs). After isolation of the genomic DNA ...
-
bioRxiv - Molecular Biology 2023Quote: ... The resulting plasmid backbone of the linearized vector was run on the 0.8% agarose gel and purified by Monarch DNA Gel Extraction Kit (NEB). The oligos for the inserted sgRNA targeting sequences were synthesized with two overhangs compatible with the linearized vector and complementary to each other ...
-
bioRxiv - Microbiology 2023Quote: ... and PacBio sequencing were prepared using the NEB Monarch HMW DNA Extraction Kit for Cells and Blood (NEB #T3050) using the manufacturer’s protocol for fresh blood with slight modifications as we have previously described (28) ...
-
bioRxiv - Genomics 2023Quote: ... DNA was extracted from 4.3×10^6 cells using the Monarch HMW DNA Extraction Kit for Cells and Blood (New England Biolabs) with 1400 rpm lysis speed ...
-
bioRxiv - Developmental Biology 2023Quote: ... Genomic DNA from the spleen was extracted using the Monarch® HMW DNA Extraction Kit for Tissue (NEB #T3060) as per manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... Genomic DNAs (gDNAs) were extracted using the Monarch HMW DNA extraction kit for Tissue from New England Biolabs (NEB) with the following modifications ...
-
bioRxiv - Genomics 2023Quote: ... Linearized destination vectors were gel purified using 0.7% agarose gels and extracted with the Monarch DNA Gel Extraction Kit (New England Biolabs), before further purification by isopropanol precipitation.
-
bioRxiv - Genomics 2023Quote: ... used to estimate conversion rates was added to each DNA extraction before shearing as per the manufacturer’s instructions (New England Biolabs). For each individual DNA extraction ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... using the Monarch® HMW DNA Extraction Kit for Tissue (Cat. No. T3060S, New England BioLabs Inc., MA, USA). DNA from resequencing samples was isolated using either a modified protocol for the DNeasy Blood and Tissue Kit (Cat ...
-
bioRxiv - Neuroscience 2023Quote: ... One piece of brain tissue (∼300 mg) was placed in 3 ml of ice-cold nuclei extraction buffer (NEB) [0.32 M sucrose ...