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Citations for New England Biolabs :
301 - 350 of 953 citations for Lupatadine fumarate EP Impurity C since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... the concentrated protein at 3 mg/mL was incubated with c-AMP Protein Kinase A PKA (NEB) (molar ratio of 40:1 ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was then reverse-transcribed for 30[min at 50°C using ProtoScript II (New England Biolabs) with a primer (5′-Phos-NNAGATCGGAAGAGCGTCGTGTAGGGAAAGAG-iSp18-GTGACTGGA GTTCAGACGTGTGCTC-3′ ...
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bioRxiv - Microbiology 2023Quote: ... The lysates (50 μl) were then incubated at 55°C with 1.25 μl proteinase K (NEB, P8107S). After 1h of incubation ...
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bioRxiv - Developmental Biology 2023Quote: ... A C-terminal HA tag was added using Q5 Site-Directed Mutagenesis Kit (New England Biolabs, #E0554S). For microinjection ...
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TAD border deletion at the Kit locus causes tissue-specific ectopic activation of a neighboring genebioRxiv - Genetics 2022Quote: ... The ligation products were reverse crosslinked at 65°C overnight with proteinase K (New England Biolabs, P8107S) and DNA was extracted with phenol-chloroform ...
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bioRxiv - Cell Biology 2023Quote: ... Reactions were performed for 30 min at 37°C in 1X T4 DNA ligase buffer (B0202S, NEB). Phosphorylated oligos were annealed by heating for 5 min at 95°C and slow cooling (0.1°C/s) ...
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bioRxiv - Genomics 2023Quote: ... Ligation was performed for 4 h at 16°C using 10,000 units of T4 DNA ligase (NEB) in 1.2 mL of ligation buffer (120 μL of 10× T4 DNA ligase buffer ...
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bioRxiv - Microbiology 2023Quote: ... and digested alongside pGS001 for 2 hours at 37°C using XhoI and XbaI (New England Biolabs). Digested plasmid backbone and PCR product were gel extracted (QIAquick Gel Extraction Kit ...
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bioRxiv - Genomics 2023Quote: ... and elongation for 7 min at 72°C) using the Phusion High-Fidelity DNA Polymerase (NEB, #M0530S) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 72°C – 1 min]) with NEBNext® High-Fidelity 2X PCR Master Mix (New England Biolabs, #M0541S) and barcoded Nextera primers (1.25 μM each ...
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bioRxiv - Genomics 2023Quote: ... an exonuclease treatment was carried out at 37°C for 1h with 10U of exonuclease I (NEB) in 1X exonuclease buffer (NEB) ...
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bioRxiv - Cell Biology 2023Quote: ... (9)C=cell barcode) was used for single-stranded synthesis and Second Strand Synthesis Module (NEB, #E6111) was used for double-stranded cDNA synthesis ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclei were centrifuged (500xg, 5 min, 4°C) and washed once with 1X NEBuffer 2.1 (NEB, #B7202). For nucleosome depletion ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Nuclei were pelleted at 500 ξ g at 4°C for 5 minutes and resuspended in 0.5 mL of 1.2x NEBuffer r2.1 (New England Biolabs) containing 3% sodium dodecyl sulfate (SDS ...
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bioRxiv - Cell Biology 2024Quote: ... Deproteinized reactions (63 μl) were incubated for 45 min at 37°C in 1X ThermoPol buffer (NEB) with 15 units E ...
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bioRxiv - Neuroscience 2024Quote: ... and 16,000 units/ml lambda protein phosphatase at 30°C for 3 hours (New England Biolabs #P753S).
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bioRxiv - Neuroscience 2024Quote: ... the samples were cleared overnight at 37°C with a Clearing Solution containing Proteinase K (NEB P8107S) and Clearing Premix (Vizgen 20300003) ...
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bioRxiv - Synthetic Biology 2024Quote: ... The resulting plasmids were digested at 37° C for 30 minutes using BamHI-HF (New England Biolabs) and subsequently purified using the QIAquick PCR Purification Kit ...
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bioRxiv - Cancer Biology 2024Quote: ... and λ-protein phosphatase for 30min at 30°C according to the manufacturer’s instructions (New England Biolabs).
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bioRxiv - Developmental Biology 2024Quote: ... and the unused barcodes were removed from the beads with exonuclease I (NEB, incubation 45min 37°C), according the Nadia protocol (Davey et al. ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Tissue samples were stored in DNA/RNA stabilizer solution at -80°C (New England Biolabs, Frankfurt, Germany) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... The ClbI C-terminal GFP fusion was constructed using the Gibson Assembly kit (New England Biolabs, MA, USA). Briefly ...
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bioRxiv - Genetics 2021Quote: ... A rolling circle amplification reaction (RCA) of partially double-stranded C-circles was performed with 0.2μg/µl bovine serum albumin (NEB), 0.1% Tween-20 (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: ... Two 100 μl aliquots were incubated overnight at 37°C with and without Endo H (3000 U, NEB). Then the samples were heated with 100 μl SDS sample buffer for 5 min at 95°C.
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bioRxiv - Cell Biology 2020Quote: ... The extract was incubated for 1 h at 4 °C with amylose resin (New England Biolabs, Ipswich, MA), loaded onto a column ...
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bioRxiv - Bioengineering 2021Quote: ... H2-Db and H2-Kb genes were engineered with a C-terminal cysteine by site-directed mutagenesis (NEB), and pMHC molecules were expressed and refolded as described previously[40] ...
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bioRxiv - Bioengineering 2021Quote: ... 4 µg plasmid was digested for 4 h at 37°C with BstXI and XhoI (New England Biolabs). Products were run on a 1% agarose gel for 30 min at 120 V ...
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bioRxiv - Neuroscience 2020Quote: ... Vector and insert were digested (30 min, 37 °C) according to manufacturer’s protocols (enzymes by New England Biolabs), either purified directly (insert ...
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bioRxiv - Biophysics 2020Quote: ... followed by treatment for 1 h at 37 °C with 2.5 units of RNase-free DNase I (NEB). The sample was once again cleaned with RNeasy MinElute Cleanup Kit before applying on a 1% agarose gel for gel extraction and purification with QIAGEN gel extraction kit and elution with RNase free H2O ...
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bioRxiv - Microbiology 2020Quote: ... followed by 10 cycles of annealing at 54°C for 30 s) using Taq polymerase (New England Biolabs) according to the manufacturer’s instructions and the primers 5-CTTGACATCTCTGAGGCAAC-3 and 5-ATGCAGGGGCAAAGTCATTAG-3 ...
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bioRxiv - Microbiology 2021Quote: ... 100ng of vector was digested for 1 hour at 37°C with 10 U of BamHI-HF (NEB) to create a single double-stranded cut or 10 U Nb.BtsI (NEB ...
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bioRxiv - Biochemistry 2022Quote: ... The unbound fraction was then incubated for 1 hour at 4 °C with lambda phosphatase (New England Biolabs), calf intestinal phosphatase (New England Biolabs) ...
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bioRxiv - Biochemistry 2021Quote: ... The E PBM C-terminal mutation (DVLL>GGGG) was performed using the Q5 site-directed mutagenesis kit (NEB). The sequences corresponding to the PDZ domains were cloned into the pCMV GFP vector using EcoRI and XhoI sites.
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bioRxiv - Molecular Biology 2020Quote: ... Around 500ng of RNA were ligated overnight at 16°C using 20units of T4 RNA ligase 1 (NEB) in the presence of 10% PEG 8000 to favour intramolecular ligations ...
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bioRxiv - Molecular Biology 2020Quote: ... Around 500ng of RNA were ligated overnight at 16°C using 20units of T4 RNA ligase 1 (NEB) in the presence of 10% PEG 8000 to favour intramolecular ligations ...
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bioRxiv - Biophysics 2020Quote: 20μg of pRBD vector was linearized with the PmeI enzyme by incubating at 37°C overnight (NEB, R0560). Enzyme was inactivated (65°C ...
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bioRxiv - Biochemistry 2020Quote: ... The transcription reaction was carried out at 37°C for 12 hours with T7 polymerase (New England Biolabs) in the presence of 13C/15N labeled nucleotide triphosphates (Cambridge Isotope Laboratories ...
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bioRxiv - Bioengineering 2021Quote: ... H2-Db and H2-Kb genes were engineered with a C-terminal cysteine by site-directed mutagenesis (NEB), and pMHC molecules were expressed and refolded as described previously (19).
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bioRxiv - Evolutionary Biology 2022Quote: ... The reaction was incubated for 1 hour at 37 °C and stopped by adding 6X loading dye (NEB) with EDTA and run 2% agarose gel ...
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bioRxiv - Genetics 2020Quote: ... Pairs of annealed oligos and the digested vector were ligated overnight at 16°C using T4 ligase (NEB). Following transformation ...
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bioRxiv - Biochemistry 2021Quote: ... also C-terminal) FT MYOC variants were generated by DNA assembly (NEBuilder HiFi DNA Assembly Cloning Kit, NEB) by combining a PCR-amplified eGLuc2 product from a pcDNA3 MYOC eGLuc2 plasmid (originally described previously8) ...
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bioRxiv - Molecular Biology 2021Quote: ... for 12 h at 4 °C with 10 μg of Factor Xa protease (New England Biolabs, Hitchin, UK) per 1 g of mitochondria ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting Gβ1γ2 was then incubated for 1 hour at 4 °C with lambda phosphatase (New England Biolabs), calf intestinal phosphatase (New England Biolabs) ...
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bioRxiv - Neuroscience 2021Quote: ... Phosphatase treated samples were enzymatically treated for 3 n at 37°C shaking (1.25 μl 10 μM MnCl2, 1.25 μl 20X NEB buffer ...
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bioRxiv - Genomics 2021Quote: ... Nicks were then ligated for 30 min at 37 °C using 4 units of Taq DNA ligase (NEB) in the presence of 0.5 μl thermopol buffer ...
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bioRxiv - Biophysics 2020Quote: ... SecA(N95) K797C was overexpressed for 4 hours at 37 °C in E.coli NiCo21 cells (New England Biolabs) grown in 2xYT (Acumedia ...
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bioRxiv - Biochemistry 2022Quote: ... The reaction was incubated at 37°C overnight and stopped by adding 2 Units of DNase I (NEB) and incubating at 37°C for 15 minutes ...
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bioRxiv - Microbiology 2022Quote: ... The digestion product was then treated with Antarctic phosphatase for 1 hour at 37°C (New England Biolabs). PCR products were purified using the Zymogen Clean and Concentrator PCR-cleanup kit (Zymo Research ...
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bioRxiv - Microbiology 2022Quote: ... Each mixture was incubated at 50°C for 30 minutes after which 0.6μL (3U) of MboI (NEB R0147) was added to each reaction ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were fragmented at 94°C for 2.75 min as per the NEBNext Magnesium RNA Fragmentation Module (NEB) with subsequent RNA purification using the Zymo RNA Clean & Concentrator kit ...