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Citations for New England Biolabs :
101 - 150 of 953 citations for Lupatadine fumarate EP Impurity C since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 5’ triphosphates on the synthesis products were converted into 5’ monophosphates: samples were treated with recombinant shrimp alkaline phosphatase (NEB) (10 µl reaction in 1× NEB buffer 2.1, 37°C 30 min, 65°C 5 min), these reactions were made up to 15 µl including 0.5 µl 10× NEB 2.1 ...
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bioRxiv - Genetics 2021Quote: ... then a final 1 min extension (72 °C)—using Phusion Flash polymerase (NEB) from cDNA clones using primers containing an EagI restriction site (forward primers ...
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bioRxiv - Developmental Biology 2021Quote: ... Restriction digest at 37°C for 20 minutes with Msp1 (New England Biolabs) was performed to cleave the wildtype ...
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bioRxiv - Developmental Biology 2020Quote: ... Chromatin was digested overnight at 37°C by DpnII (New England Biolabs,. R0543M). The digestion was monitored by specific primers spanning DpnII sites in the genome (Supplemental Table 3)
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bioRxiv - Microbiology 2020Quote: ... incubated overnight at 4°C and treated with DNAse I (New England Biolabs) at 37°C for 60 minutes ...
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bioRxiv - Genomics 2020Quote: ... and then held at 4°C until a subsequent T7 Endonuclease I (NEB) treatment at 37°C for 30 to 90 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... and 5 μg were digested overnight at 37°C with FspEI (NEB, R0662S), which recognizes CMC sites and creates a double-stranded DNA break on the 3’ side of the modified cytosine at N12/N16 ...
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bioRxiv - Genomics 2021Quote: ... for 1 hour at 37°C in the presence of CutSmart buffer (NEB). To achieve combined DNA purification and selection of high molecular weight fragments ...
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bioRxiv - Genomics 2021Quote: ... Extracted genomic DNA was digested overnight at 37°C with DpnI (NEB, R0176S) to cut adenine-methylated GATC sites ...
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bioRxiv - Cell Biology 2021Quote: ... and lysate was incubated 37°C 60 min in 2 units CIP (NEB) per 50 μL reaction containing 50μg of total protein ...
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bioRxiv - Neuroscience 2021Quote: ... The PCR reaction was DpnI digested (New England Biolabs, 37°C, 1 h) and column purified (Macherey-Nagel ...
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bioRxiv - Bioengineering 2020Quote: ... IVT was performed overnight at 37°C using the HiScribe T7 kit (NEB) following the manufacturer’s instructions (N1-methyl-pseudouridine modified) ...
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bioRxiv - Cell Biology 2022Quote: ... for 10 mins at 100°C and then resuspended in EndoH (NEB, P0702S) for 1.5 hours at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... B and C oligonucleotides were phosphorylated with T4 polynucleotide kinase (NEB, Cat #M0201L) according to the manufacturer recommendations ...
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bioRxiv - Genomics 2022Quote: ... ligated overnight at 16°C and subsequently transformed into competent cells (NEB C2992H).
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bioRxiv - Immunology 2022Quote: ... and C-TBGH fragments using NEBuilder HIFI DNA Assembly Mastermix (New England BioLabs). Assembled cassettes were amplified using CMV_FWD and TBGH_REV primers ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 hour at 37 °C and SfiI (New England Biolabs, catalog #R0123) for 5 hours at 50 °C ...
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bioRxiv - Synthetic Biology 2022Quote: ... into backbones previously digested with corresponding restriction enzymes (NEB, 1 h 37° C) to yield intermediate plasmids containing individual transcriptional units ...
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bioRxiv - Molecular Biology 2023Quote: ... fully C-methylated XP12 phage DNA (Dr. Yan-Jiun Lee, NEB Ipswich MA), fully C-hydroxymethylated T414743 and fully C-hydroxy-methyl-beta-glucosylated T4 alpha-glucosyl-transferase knockout (AGT- ...
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bioRxiv - Genomics 2024Quote: ... PCR products were then digested at 37°C with DpnI (New England Biolabs) for 2 hours to remove residual starting plasmid ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli and grown at 30°C to ensure construct stability (New England Biolabs). Plasmid clones were picked and inoculated into liquid LB media for overnight culture followed by miniprep using the PureLink Plasmid MiniPrep Kit (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... for 60 min at 37 °C and proteinase K (New England Biolabs, P8107S) for 3 hours at 55 °C ...
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bioRxiv - Microbiology 2022Quote: ... The extension products were then C-tailed using terminal transferase (New England Biolabs). The resulting C-tailed extension products were then amplified using primers CDEP4816 and P1 or CDEP4816 and P3 to generate barcoded amplicon libraries for Illumina sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was phosphorylated for 1h at 37°C with T4 Polynucleotide kinase (NEB or made in-house by the Molecular Biology Service ...
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bioRxiv - Molecular Biology 2023Quote: ... the phosphorylated ssDNA was digested overnight at 37°C with Lambda exonuclease (NEB or made in-house by the Molecular Biology Service ...
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bioRxiv - Molecular Biology 2023Quote: Hi-C libraries were treated with 3 U of T4 DNA polymerase (NEB) in the presence of 0.1 mM dGTP (NEB ...
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bioRxiv - Genomics 2022Quote: ... chromatin was digested overnight at 37°C with 50 U Dpn II (NEB) in each tube ...
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bioRxiv - Genetics 2023Quote: ... and then digested overnight with DpnI at 37° C (NEB; cat. no. R0176S). 3 μL of digested linear PCR product was transformed into NEB stable competent E ...
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bioRxiv - Molecular Biology 2023Quote: ... digested O/N at 42°C with 3U β-agarase (New England Biolabs) and again for 2 hrs with 2U β-agarase ...
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bioRxiv - Plant Biology 2023Quote: ... and ligated overnight at 16°C using T4 ligase (NEB, Ipswich, Massachusetts, USA). The fully reassembled BdPOX gene was ligated overnight at 16°C with the previously digested (FastDigest Eco31I ...
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bioRxiv - Systems Biology 2023Quote: ... and 72°C for 5 min) (Phusion High-Fidelity DNA Polymerase, NEB, M0530S) using the P7 and a T7-fused P5 primer (5′ TAA TAC GAC TCA CTA TAG GGA ATG ATA CGG CGA CCA CCG A 3′ ...
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bioRxiv - Genomics 2023Quote: ... RCA was performed at 30°C overnight using Phi29 enzyme mix (NEB, M0269L). fluorescent probes hybridization was done at 2X SCC and 10% formamide buffer mix ...
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bioRxiv - Cancer Biology 2023Quote: ... at 37°C for 2hrs and then treated with Proteinase K (NEB, #P8107S) at 55°C for 2 hrs ...
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bioRxiv - Systems Biology 2024Quote: ... then digested for 1 hour at 37 °C using SacI (New England Biolabs). This linearized plasmid was used for Gibson assembly with an insert composed of a 248 base pair region of PBS2 amplified from genomic DNA ...
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bioRxiv - Genomics 2024Quote: ... at 65 ⁰C for 2 h followed by MspI treatment (NEB Ipswich, MA) at 37⁰C overnight ...
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bioRxiv - Microbiology 2024Quote: ... PCRs were digested overnight at 37 °C with DpnI (New England Biolabs, UK). Amplicons or digest reactions were purified using the Wizard SV Gel and PCR Clean-Up System (Promega ...
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bioRxiv - Neuroscience 2024Quote: ... C-terminal GFP sequence via Gibson Assembly (New England Biolabs, Inc. Ipswich, MA). pUASTattB(pHluorin-HTTex1Q25-tdTomato ...
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bioRxiv - Genetics 2024Quote: ... We digested the PolyA Minus library with Kpn I (NEB, 37°C o.n.), followed by gel purification and digestion with BamHI in the presence of Shrimp Phosphatase (NEB M0371S ...
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bioRxiv - Cancer Biology 2024Quote: ... the PCR product was digested overnight at 37⁰C with PstI (NEB R0140S) and the resulting digestion products were resolved on an agarose gel.
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bioRxiv - Developmental Biology 2024Quote: ... Ligation was done overnight at 16°C using T4 ligase (NEB cat #M0202M). Sonication was done using Covaris onetube-10 AFA strips using the following parameters for a 300bp fragment size (Duration ...
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bioRxiv - Cell Biology 2020Quote: ... 800ng were treated for 20’ at 37°C with 50u ShortCut Rnase III (NEB), in the digestion buffer supplied by the manufacturer ...
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bioRxiv - Developmental Biology 2021Quote: ... and 2 hours at 55°C with 0.1 mg/ml Proteinase K (NEB P8107S).
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bioRxiv - Molecular Biology 2020Quote: ... digested overnight at 50°C with 1 μl 20 U/μl SfiI (NEB R0123S) in 200 μl 1x CutSmart buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... digested overnight at 50°C with 1 μl 20 U/μl SfiI (NEB R0123S) then rinsed with 1xTE.
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bioRxiv - Molecular Biology 2021Quote: ... Ligation was conducted overnight at 16 °C with T4 DNA Ligase (New England Biolabs). All minigene mutations were introduced via Q5 Site-Directed Mutagenesis Kit (New England Biolabs) ...
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bioRxiv - Genomics 2020Quote: ... with BspQ1 overhangs were ligated using T7 DNA ligase (NEB M0318; 16°C overnight) into BspQ1 digested (NEB R0712 ...
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bioRxiv - Plant Biology 2021Quote: ... Overnight digestion at 37 °C was performed using 400U of Hind III enzyme (NEB). Digested DNA was ligated during 5 h incubation at 16 °C with 100 U of T4 DNA ligase (NEB) ...
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bioRxiv - Cancer Biology 2021Quote: ... 40 μg of Hi-C library DNA were incubated with T4 DNA polymerase (NEB) for 4 hours at 20°C to remove of biotin from non-ligated fragment ends ...
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bioRxiv - Systems Biology 2020Quote: ... The resulting PCR products were digested for 1h at 37°C with DpnI (NEB) and transformed in competent Escherichia coli MC1061 cells ...
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bioRxiv - Microbiology 2021Quote: ... the samples were incubated at 37°C overnight with PNGase F (New England BioLabs) to release the glycans ...