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Citations for New England Biolabs :
3401 - 3450 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... PCR products were purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs). RT ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR products were converted to RNA using the T7 RNA Transcription Kit (NEB) and purified with RNEasy Kit (Qiagen ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA synthesis was performed using LunaScript™ RT SuperMix Kit (New England Biolabs) and in a standard thermocycler according to manufacturer instructions ...
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bioRxiv - Genomics 2019Quote: ... and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760S). Libraries were assessed for quality control on the Agilent Bioanalyzer 2100 ...
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bioRxiv - Synthetic Biology 2019Quote: ... PCR amplifications were column-purified using the Monarch PCR & DNA Cleanup Kit (NEB) following manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... cDNA was synthesized from RNA using Protoscript II Reverse Transcriptase Kit (NEB; E6560L). Real-time PCR (qPCR ...
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bioRxiv - Biophysics 2019Quote: ... Libraries were prepared using the NEBNext Ultra II DNA library Prep kit (NEB) for both IPs and corresponding inputs ...
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bioRxiv - Genetics 2019Quote: ... and cDNA libraries were prepared using the Directional RNA Library Prep Kit (NEB). Paired end sequencing was done on a NoaSeq 6000 ...
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bioRxiv - Genetics 2020Quote: ... rnr1Y285F and rnr1Y285A using the Q5 site-directed mutagenesis kit (New England Biolabs) (Table S3) ...
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bioRxiv - Genetics 2020Quote: ... Libraries were prepared with the NEBNext Ultra II Library Preparation Kit (NEB #E7103). Samples were sequenced on a combination of Illumina’s NovaSeq 6000 and HiSeq 4000 at Novogene.
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bioRxiv - Genetics 2021Quote: ... library construction was performed using the NEBNext Ultra-II kit (New England Biolabs) following manufacturer recommendations ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and prepared using NEBNext Ultra II Directional RNA Library Prep Kit (NEB #E7765) and single indexing from the NEBNext Multiplex Oligos kit (NEB #E7555L ...
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bioRxiv - Physiology 2021Quote: ... Synthesis of cDNA was performed with the LunaScript RT SuperMix Kit (NEB, #E3010L). For quantitative real-time PCR (qPCR ...
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bioRxiv - Developmental Biology 2020Quote: ... cDNA was generated using Protoscript II First Strand Synthesis Kit (New England Biolabs). Lmo7a rescue constructs and lmo7a:sfGFP fusion constructs were generated by Gibson Assembly (Gibson et al ...
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bioRxiv - Microbiology 2021Quote: ... and re-cloned into the vector using the NEBuilder kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... were transcribed in vitro using an EnGen® sgRNA Synthesis Kit (NEB, E3322S). The generation and characterization of CRTD5 hiPSCs (hPSCreg ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids expressing mgrB variants were constructed using Q5 site-directed mutagenesis kit (NEB). All constructs were confirmed by DNA sequencing.
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bioRxiv - Immunology 2022Quote: ... cDNA libraries were prepared using NEBNext Ultra DNA library kits (New England BioLabs) for Illumina ...
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bioRxiv - Cancer Biology 2021Quote: ... using a Gibson Assembly® Cloning Kit (New England BioLabs; Cat. No. E2611). The library was produced in MegaX DH10B T1R Electrocomp Cells (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... RNA was isolated using the Monarch Total RNA miniprep kit (New England BioLabs), and equivalent amounts of RNA were used to synthesize cDNA using the SuperScript III First-Strand Synthesis System (Invitrogen) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The ligation product was purified using the Monarch PCR & DNA Cleanup Kit (NEB) eluting the product in 10 μL ...
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bioRxiv - Genetics 2020Quote: ... and purified with Monarch PCR & DNA Cleanup Kit (5 μg) (NEB, Ipswich, USA), checked on an 1 % (w/v ...
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bioRxiv - Genomics 2020Quote: ... Libraries were generated using the NEBNext Ultra II DNA library prep kit (NEB) and dual indexed ...
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bioRxiv - Microbiology 2021Quote: ... ribosomal RNA was depleted using NEBNext rRNA Depletion Kit (Bacteria) (New England Biolabs) with additional organism-specific spiked-in custom probes following the manufacturer’s protocols ...
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bioRxiv - Immunology 2021Quote: ... and cDNA was prepared with AMV First Strand cDNA kit (New England Biolabs) using oligo-dT to prime the reaction ...
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bioRxiv - Immunology 2020Quote: ... we used the Luna Universal Probe Onestep RT-qPCR kit (New England Biolabs) with 1 ug of RNA ...
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bioRxiv - Molecular Biology 2021Quote: ... The Luna Universal Probe One-Step RT-qPCR Kit (Cat. No. E3006S, NEB) was used as the relevant master mix ...
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bioRxiv - Immunology 2020Quote: ... and qPCR was performed using SYBR Green (Dynamo HS kit; New England Biolabs) kit in a QuantStudio™ 3 Reak-Time PCR Instrument (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... and RNA transcribed using a HiScribe T7 High Yield RNA Synthesis kit (NEB) following the manufacturers’ protocol ...
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bioRxiv - Genomics 2021Quote: Gibson assembly (NEBuider Hifi DNA Assembly Cloning kit, New England Biolabs, Ipswich, Massachusetts) and mutagenesis primers were used to generate mutated flag-ADAR mammalian expression vectors ...
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bioRxiv - Genetics 2019Quote: ... and reverse transcription was performed using LunaScript RT Supermix Kit (New England Biolabs). Oligonucleotides used for qRT-PCR are listed in Supplementary Information Table S3 ...
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bioRxiv - Genetics 2020Quote: ... following rRNA depletion using a NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB).
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bioRxiv - Genetics 2020Quote: ... and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760). Then were pooled for deep sequencing by using Illumina Hiseq Xten (2 × 150 ...
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bioRxiv - Microbiology 2021Quote: ... The RT-qPCRs with Luna Universal One-Step RT-qPCR kit (NEB, USA) were performed according to the protocol of the manufacturer ...
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bioRxiv - Molecular Biology 2021Quote: ... NEBNext Ultra II RNA Library Prep Kit (Catalog: Catalog: E7775S, New England Biolabs) for Illumina was used for the library preparation ...
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bioRxiv - Microbiology 2021Quote: ... by using the NEBuilder HiFi DNA Assembly kit (New England Biolabs, Ipswich, MA). The cDNA sequence of DENV-4M in the full-length clone was finally validated by Sanger sequencing using the primers listed in Table S2.
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bioRxiv - Cancer Biology 2019Quote: ... Universal sequencing adaptor were added using the NEBnext Ultra DNA Library Kit (NEB) according to the supplier’s recommendation ...
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bioRxiv - Immunology 2022Quote: ... and/or site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs). Compared with the Wu01 strain spike protein amino acid sequence ...
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bioRxiv - Microbiology 2022Quote: ... 1800 bp was excised and processed using Monarch DNA Gel Extraction kit (NEB). Purification kits were used according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... The three fragments were assembled with a Gibson Assembly Kit (New England Biolabs).
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bioRxiv - Molecular Biology 2022Quote: In vitro translation was carried out using the deltaRF123 Kit (New England Biolabs). 200 ng of mRNA was added per μL of reaction ...
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bioRxiv - Molecular Biology 2022Quote: ... reverse transcription was performed using ProtoScript II First Strand cDNA Synthesis Kit (NEB) using d(T)23VN primer with DNase I (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... the stop codon was deleted using the Q5 Site-Directed Mutagenesis Kit (NEB). Plasmids were transformed into E ...
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bioRxiv - Bioengineering 2022Quote: ... RNA was subsequently purified using Monarch® RNA Cleanup Kit (New England Biolabs).
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bioRxiv - Cell Biology 2022Quote: RNA extraction was performed using the Monarch Total RNA miniprep kit (T2010S, NEB), following the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2022Quote: ... and mutations were introduced using the Q5 site-directed mutagenesis kit (NEB Biolabs). Non-cleavable His-Ub construct was derived from a previously described His-Ub construct6 with the 3C protease site removed by Q5 site-directed mutagenesis.
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bioRxiv - Biochemistry 2022Quote: ... and mutations were introduced using the Q5 site-directed mutagenesis kit (NEB Biolabs). Non-cleavable His-Ub construct was derived from a previously described His-Ub construct6 with the 3C protease site removed by Q5 site-directed mutagenesis.
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bioRxiv - Biochemistry 2022Quote: ... The amplicons were gel-extracted using the Monarch DNA Gel Extraction kits (NEB). NGS-based amplicon sequencing was carried out for all libraries at Genewiz UK Ltd using the Amplicon-EZ service ...
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bioRxiv - Bioengineering 2022Quote: ... RNA was subsequently purified using Monarch® RNA Cleanup Kit (New England Biolabs).
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bioRxiv - Genomics 2022Quote: ... then purified using the Monarch PCR&DNA Cleanup kit (New England BioLabs T1030L). Finally ...