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Citations for New England Biolabs :
3251 - 3300 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Illumina libraries were constructed using the NEBNext Ultra II kit (New England Biolabs) and sequenced on an Illumina HiSeq2500 ...
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bioRxiv - Cell Biology 2022Quote: ... and adapter ligation were performed by using RNA library prep kit (E7530L, NEB). DNA products were cleaned using AMPure XP beads (Beckman) ...
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bioRxiv - Genomics 2022Quote: ... DNA was purified using a Monarch PCR & DNA Clean up kit (NEB, T1030) and eluted in 12 μL of water.
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bioRxiv - Genomics 2022Quote: The ligation reaction was carried out using Quick Ligation™ kit (NEB, M2200). A 10 μl mixture consist of 1 μl of 1 μM N40+22nt DNA (0.1 μM final) ...
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bioRxiv - Genomics 2020Quote: ... Libraries were generated using NEBnext Ultra DNA library preparation kit for Illumina (NEB). Libraries were sequenced by paired-end sequencing using a NextSeq 500 (Illumina).
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bioRxiv - Microbiology 2020Quote: ... the source plasmid (p-retronWT) was mutagenized through PCR using a kit (NEB; Q5-Site-Directed Mutagenesis Kit ...
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bioRxiv - Molecular Biology 2021Quote: All mutagenesis was done using Q5 Site-directed Mutagenesis Kit Protocol (NEB #E0554S) according to the manufacturer’s instructions using primers in table T1 ...
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Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: ... gel purifications were performed using the Monarch DNA Gel Extraction Kit (NEB T1020L), and minipreps were performed using the Monarch Plasmid Miniprep Kit (NEB T1010L) ...
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bioRxiv - Molecular Biology 2021Quote: ... NGS libraries were made using NEBNext Ultra II DNA Library Prep kit (NEB). DNA sequencing was performed on Illumina MiniSeq with 150+150 bp paired-end protocol.
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bioRxiv - Cancer Biology 2019Quote: ... Variants were generated using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Plasmid transfection was performed with Lipofectamine 3000 (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... and reverse-transcripted to cDNA using LunaScript RT SuperMix kit (New England Biolabs) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... PCR was done with the Phusion High-Fidelity PCR kit (New England BioLabs) with 40ng of gDNA ...
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bioRxiv - Plant Biology 2019Quote: TNIC317S active site mutant was generated using Q5 Site-Directed Mutagenesis Kit (NEB).pGEM-T-TNI served as a template for making the site directed mutant using primers P2346 and P2347 ...
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bioRxiv - Genomics 2019Quote: ... the DNA was treated with repair enzymes (New England Biolabs PreCR kit M0309) and purified using Qiagen PCR purification columns as above.
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bioRxiv - Microbiology 2019Quote: cDNA synthesis reactions were carried out using the Lunascript RT Supermix kit (NEB) and 500 ng of RNA was used for each reaction.
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bioRxiv - Evolutionary Biology 2019Quote: ... CPS mutant plasmids were constructed using the Q5 Site-Directed Mutagenesis Kit (NEB).
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bioRxiv - Cancer Biology 2019Quote: ... SPA4CT-T43P was generated using the Q5 Site-Directed Mutagenesis Kit (NEB E0554S) using pLVX SPA4CT as a template ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA samples were depleted of rRNA using the NEBNext rRNA Depletion kit (NEB). RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep kit (NEB) ...
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bioRxiv - Synthetic Biology 2019Quote: ... For cloning purposes DNA was purified using Monarch PCR & DNA Cleanup Kit (NEB) and assembled using NEBuilder HiFi DNA Assembly Cloning Kit (NEB) ...
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bioRxiv - Physiology 2020Quote: ... according to the manufacturer’s recommendations (Magnetic mRNA Isolation Kit protocol from NEB, S1550S), and eluted in 17 μL ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and prepared using NEBNext Ultra II Directional RNA Library Prep Kit (NEB #E7765) and dual indexing from the NEBNext Multiplex Oligos kit (NEB #7600 ...
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bioRxiv - Developmental Biology 2020Quote: ... mRNA was captured using NEBNext (Poly A) mRNA magnetic isolation kit (NEB, E7490) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... A Q5® site-directed mutagenesis kit (New England BioLabs, Ipswich, MA, USA) with forward primer 5’-CTACAGAAGAAAGCACAATG −3’ and reverse primer 5’-AAGGTAAAAGAGAGATTTTGAC −3’ was used to delete the stop codon in PIWIL3 ...
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bioRxiv - Microbiology 2019Quote: ... Reverse transcription was performed using ProtoScript First Strand cDNA Kit (New England Biolabs) according to the manufacturer’s instructions for the synthesis of first strand cDNA ...
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bioRxiv - Genomics 2019Quote: ... RNA was extracted from clones using a Monarch Total RNA extraction kit (NEB). cDNA was synthesized using MaximaH RT (NEB ...
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bioRxiv - Immunology 2019Quote: ... followed by site-directed mutagenesis using the Q-5 kit (New England Biolabs) in order to generate point mutations into MIC1 (MIC1-T126A/T220A ...
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bioRxiv - Systems Biology 2019Quote: ... then in vitro transcription further amplified the oligos (NEB Quick High Yield Kit), t7 reactions were purified with desalting columns ...
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bioRxiv - Biochemistry 2019Quote: FXN variants were constructed using the Q5® Site-Directed Mutagenesis Kit (NEB) and confirmed by sequencing of plasmid DNA and intact mass spectrometry of purified proteins ...
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bioRxiv - Genetics 2021Quote: ... PCR amplicons were purified using the Monarch DNA Gel Extraction Kit (NEB, T1020S) and subjected to sequencing.
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bioRxiv - Genetics 2020Quote: ... Site directed mutagenesis using the Q5 Site-Directed Mutagenesis kit (New England Biolabs) and AP2S1 specific primers (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760). A total of 20 libraries were constructed [including ...
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bioRxiv - Genetics 2020Quote: ... samples were purified by the Monarch PCR & DNA Cleanup Kit (New England Biolabs). For the library preparation ...
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bioRxiv - Cell Biology 2021Quote: ... and blunt and phosphorylated ends were generated using the Quick Blunting kit (NEB), before removal of DNA fragments under 100 bp using AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Immunology 2021Quote: ... Luna Universal Probe One-step RT-qPCR kit (New England Biolabs, Ipswich, MA) was used to amplify the envelope (E ...
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bioRxiv - Genomics 2020Quote: ... We used the NEBNext Ultra DNA Library Prep Kit (New England Biolabs, USA) to generate Illumina sequencing libraries from sheared DNA samples ...
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bioRxiv - Immunology 2021Quote: ... and then transcribed with HiScribe™ T7 High Yield RNA Synthesis Kit (NEB) [23] ...
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bioRxiv - Plant Biology 2020Quote: ... The NEBNext Ultra™ II FS DNA Library Prep Kit (New England Biolabs) was used for fragmentation/adaptor ligation and indexing of samples ...
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bioRxiv - Bioengineering 2020Quote: ... and polyadenylated using HiScribe ARCA T7 mRNA Kit (with tailing) (New England Biolabs) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... C4 domain sequence was removed with the Q5 site-directed mutagenesis kit (NEB). Mouse prolyl 4-hydroxylase alpha- and beta-chain (P4HA1 and P4HB ...
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bioRxiv - Cancer Biology 2020Quote: ... Site-directed mutagenesis was performed using Q5 Site-Directed Mutagenesis Kit (NEB, E0552S). Retrovirus was generated by transfection of Phoenix cells with 3:1 ratio of target plasmid ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA was prepared using LunaScript™ RT SuperMix Kit (New England Biolabs, E3010S). Real-time PCR was performed using Luna® Universal qPCR Master Mix (New England Biolabs ...
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bioRxiv - Cell Biology 2021Quote: ... Point mutations were made using the Q5® Site-Directed Mutagenesis Kit (NEB). Expi293FTM cells were maintained in Expi293 Expression medium and cultured under 7% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... Expression plasmids were assembled using the Gibson assembly kit (New England Biolabs, U.S.A) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... cGTP_for and cGTP_rev using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) according to the manufactureŕs protocol ...
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bioRxiv - Cell Biology 2021Quote: ... and NEBNext Ultra II RNA Library Prep Kit for Illumina® (NEB, #E7775) was then used to prepare libraries for paired-end (2×150 bp ...
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bioRxiv - Microbiology 2021Quote: ... RNA libraries were prepared using NEBNext Ultra Directional RNA Library Prep Kit (NEB). Quality of the total RNA ...
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bioRxiv - Biochemistry 2021Quote: ... and point mutations were performed using the Q5 site-directed mutagenesis kit (NEB). Proteins were expressed in E ...
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bioRxiv - Biochemistry 2020Quote: ... cDNA was obtained using LunaScript™ RT SuperMix Kit (New England Biolabs, USA) and qPCR reactions were performed using Luna® Universal qPCR Master Mix (New England Biolabs ...
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bioRxiv - Plant Biology 2021Quote: ... These DNA fragments were assembled using NEBuilder HiFi Assembly Cloning Kit (NEB, E2621) into ApaI-digested pJN105ΔpBAD ...
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bioRxiv - Systems Biology 2021Quote: ... Fragments were gel purified using the NEB Monarch gel extraction kit (NEB T1020S). Following gel extraction ...