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Citations for New England Biolabs :
3201 - 3250 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Digested products were cleaned using Monarch® PCR & DNA Cleanup Kit (NEB) and incubated with T4 DNA ligase (NEB ...
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bioRxiv - Microbiology 2024Quote: ... and cleaned with the Monarch RNA Cleanup Kit (NEB, Ipswich, MA, USA). Ribosomal 23S and 16S samples were prepared by amplifying the appropriate sequences from genomic E ...
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bioRxiv - Biochemistry 2024Quote: ... Transcription was performed using T7 HiScribe RNA synthesis kit (New England Biolabs). Generated RNA was subjected to DNase treatment (TURBODNase ...
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bioRxiv - Cancer Biology 2024Quote: ... using the NEBNext® Ultra TM II DNA Library Prep Kit (NEB E7645S/L ...
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bioRxiv - Microbiology 2024Quote: ... followed by enzyme removal via Monarch RNA Cleanup Kit (New England Biolabs). cDNA was generated from 1 µg RNA using LunaScript RT SuperMix Kit (New England Biolabs) ...
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bioRxiv - Molecular Biology 2024Quote: ... Then we used ProtoScript® II First Strand cDNA Synthesis Kit (NEB) and 3′-RACE CDS primer (ClonTech ...
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bioRxiv - Microbiology 2024Quote: Total RNA samples were treated with a NEBNext rRNA depletion kit (NEB) to remove rRNA ...
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bioRxiv - Molecular Biology 2024Quote: ... and purified with the Monarch Total RNA Miniprep Kit (New England Biolabs). Alkylated RNA was purified with the Monarch RNA Cleanup Kit (New England Biolabs) ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized using LunaScript RT SuperMix Kit (New England Biolabs, USA). SYBR green assay was used to determine HBV pregenomic expression (Luna Universal qPCR Master Mix ...
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bioRxiv - Molecular Biology 2024Quote: ... then purified using the Monarch RNA Cleanup Kit (T2040L, New England Biolabs). Quality and size of the produced RNAs were assessed using an Agilent Fragment Analyzer.
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bioRxiv - Genetics 2024Quote: All plasmids were constructed using Hifi DNA Assembly Cloning Kit (NEB, USA) and then midipreped with ZymoPure Midiprep Kit (Zymo Research ...
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: Total RNA was extracted with Monarch Total RNA Miniprep Kit (NEB #T2010S). The integrity of the RNA was determined using the TapeStation 4150 (High Sensitivity RNA kit ...
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: ... The RNA samples were cleaned with Monarch RNA Cleanup Kit (NEB #T2030) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... mRNAs were purified using the Monarch RNA Cleanup Kit (New England BioLabs) and eluted in RNAse-free water ...
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bioRxiv - Cell Biology 2024Quote: ... and NEBNext Ultra TMII Directional RNA Library Prep Kit (New England Biolabs). Libraries were sequenced with an Illumina NovaSeq 6000 to obtain 150 bp ×2 paired-end reads ...
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bioRxiv - Microbiology 2024Quote: ... and HiFi DNA Assembly kit were purchased from New England Biolabs (NEB). All bacterial strains were PCR or sequence verified before use ...
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bioRxiv - Microbiology 2024Quote: ... using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs, E0554S). Homology-directed repair templates were PCR amplified using the CloneAmp™ HiFi PCR Premix (Takara Bio ...
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bioRxiv - Microbiology 2024Quote: ... The Primer-free LunaScript® RT Master Mix Kit (New England Biolabs) was used for first strand cDNA synthesis using a gene-specific primer targeting the mCherry gene in the genomic vRNA (primer sequence 5’-CGCTTCAAGGTGCACATGG-3’) ...
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bioRxiv - Genomics 2024Quote: ... Genomic DNA was extracted using the Monarch gDNA Purification Kit (NEB T3010S) following the standard protocol but with elution using 175 µL 25 mM K3BO3 at pH 7.0.
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bioRxiv - Cell Biology 2024Quote: ... and plasmids were purified using a Monarch Plasmid Miniprep Kit (NEB #T1010). Successful mutagenesis was verified through DNA sequencing (Plasmidsaurus).
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bioRxiv - Cell Biology 2024Quote: ... and plasmids were purified using a Monarch Plasmid Miniprep Kit (NEB #T1010). Constructs were then verified by restriction digest using SacI-HF (NEB #R3156 ...
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bioRxiv - Developmental Biology 2024Quote: ... Plasmid DNA was extracted using the Monarch Miniprep Kit (NEB, Cat. #T1010L) and sent to sequencing to identify mutations.
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bioRxiv - Developmental Biology 2024Quote: ... PCR products were purified using the Monarch PCR Cleanup Kit (NEB, T1030L) and digested with the TfiI restriction enzyme (NEB ...
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bioRxiv - Developmental Biology 2024Quote: ... using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs, Ipswich, MA).
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bioRxiv - Developmental Biology 2024Quote: ... using the NEBuilder HiFi DNA Assembly Kit (New England BioLabs, Ipswich, MA) and recombined into a UASp-attB destination vector (gift of F ...
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bioRxiv - Developmental Biology 2024Quote: ... using the NEBuilder HiFi DNA Assembly Kit (New England BioLabs, Ipswich, MA).
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bioRxiv - Developmental Biology 2024Quote: ... PCR products were purified using the Monarch PCR Cleanup Kit (NEB, T1030L). PCR products were A-tailed ...
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bioRxiv - Cell Biology 2024Quote: ... The RNA was then made using a T7 Highscribe kit (NEB #E2040S), treated with DNase I (NEB #M0303L ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were prepared with the NEBNext Ultra II Directional RNA Kit (NEB). Samples were equimolarly pooled and sequenced on a NextSeq550 system (Illumina ...
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bioRxiv - Cell Biology 2024Quote: ... The insertion was done using the Q5 Site-Directed Mutagenesis Kit (NEB) and design of respective primers using the NEBaseChanger online tool (http://nebasechanger.neb.com/).
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bioRxiv - Cancer Biology 2020Quote: ... Protein amounts of OP18 and beta-actin were quantified using a primary stathmin polyclonal antibody (1:1000; Cell Signaling Technology, NEB GmbH, Frankfurt/Main, Germany) and a polyclonal beta-actin antibody (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... total RNA was collected and prepared using Monarch Total RNA Miniprep Kits (NEB). For SARS-CoV-2 experiments ...
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bioRxiv - Plant Biology 2020Quote: ... followed by subcloning into pEAQ-HT vector using the Gibson assembly kit (NEB). Linearization of pEAQ-HT vector was performed by XhoI and AgeI restriction enzyme double digestion ...
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bioRxiv - Plant Biology 2019Quote: ... The libraries were prepared using the Next Ultra RNA Library Prep Kit (NEB) by the company Novogene (China) ...
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bioRxiv - Microbiology 2020Quote: ... chloramphenicol or apramycin resistance cassette by using the Gibson isothermal assembly kit (NEB) followed by PCR amplification using the primers from the extremities ...
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bioRxiv - Microbiology 2020Quote: ... followed by vector-insert ligation using the Quick Ligation kit (New England Biolabs) to generate the original and mutated pCAGGS-HHRz-3M-eGFP-5M vectors ...
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bioRxiv - Cell Biology 2019Quote: ... rRNA was depleted using the NEBNext rRNA Depletion Kit (E6310, New England Biolabs) and subsequently directional RNA-seq libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (E7420 ...
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bioRxiv - Immunology 2021Quote: ... The released DNA was cleaned up using Monarch DNA PCR Clean Kit (NEB) and eluted in TE buffer ...
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bioRxiv - Genetics 2021Quote: SpyCas9 tyr and tbxta sgRNAs were synthesized using EnGen sgRNA Synthesis Kit (NEB). SauCas9 ...
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bioRxiv - Developmental Biology 2021Quote: ... The amplicon was purified using the Monarch PCR and DNA Cleanup Kit (NEB), and 1 ug used as template for transcription of digoxigenin labelled riboprobes using the DIG RNA Labelling mix (Sigma).
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bioRxiv - Genetics 2021Quote: ... The NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) was used for library preparation ...
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bioRxiv - Microbiology 2019Quote: ... and quantified using a NEBNext Library Quant kit for Illumina (New England BioLabs). Pooled libraries were then sequenced on a MiSeq platform (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: ... rad27 mutant alleles were constructed with the Q5 mutagenesis kit (New England Biolabs) using pEAA720 as template The oligonucleotides used to make the alleles are shown in Table S7) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA libraries were constructed using NEBNext DNA Library Prep Kits (New England Biolabs). Samples were processed and sequenced at Novogene (Hong Kong ...
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bioRxiv - Developmental Biology 2020Quote: cDNA was synthesized using the ProtoScriptII First Strand cDNA synthesis kit (NEB E6560S). Cloning of the full length admats9 from pooled cDNA from 3 ...
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bioRxiv - Developmental Biology 2021Quote: ... They generated mRNA libraries with NEB RNA Ultra kit (New England Biolabs E7770L) and dToligos probe were used to target mRNA for cDNA synthesis ...
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bioRxiv - Bioengineering 2022Quote: ... Amplicons were then indexed using the NEBNext Multiplex Oligos for Illumina kit (NEB). Amplicons were then pooled and sequenced using a Miseq Nano with paired end 150 bp reads ...
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bioRxiv - Molecular Biology 2020Quote: ... by using NEBuilder® HiFi DNA Assembly Cloning Kit (New England Biolabs, E5520S).
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bioRxiv - Molecular Biology 2021Quote: ... The next day samples were purified using PCR cleanup kit columns (Monarch, NEB) and eluted using 50 μl kit elution buffer.
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bioRxiv - Molecular Biology 2021Quote: ... Libraries were prepared using NEBnext Ultra DNA library prep kit for Illumina (NEB). Samples were paired-end sequenced at the iGE3 genomics platform of the University of Geneva.