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Citations for New England Biolabs :
3251 - 3300 of 9843 citations for Swine IL 4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... coli MG1655 genomic DNA into pKK223-3 by NEBuilder HiFi DNA Assembly Cloning Kit (NEB) and the first 300 bp portion of leuA was translationally fused with the nano-Luc (nLuc ...
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bioRxiv - Microbiology 2021Quote: The pCDNA3.1-hACE2-FLAG plasmid and a Q5 Site-Directed Mutagenesis Kit (New England Biolabs) were used according to the manufacturer’s specifications to generate recombinant plasmids that expressed hACE2 N-glycosylation mutants ...
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Factors required for adhesion of Salmonella enterica serovar Typhimurium to lettuce (Lactuca sativa)bioRxiv - Microbiology 2020Quote: ... Amplified products were reassembled by site-directed mutagenesis (SDM) kit according to manufacturer’s protocol (NEB). Sequence-confirmed plasmids were electroporated in STM SR11 Δ12.
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bioRxiv - Molecular Biology 2021Quote: ... followed by plasmid DNA isolation using the Monarch® Plasmid Minprep kit (New England Biolabs) or a NucleoBond® Xtra Midi kit (Machery-Nagel) ...
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bioRxiv - Molecular Biology 2021Quote: ... PCRs were performed using the Q5® High-Fidelity DNA Polymerase kit (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Specific primers were designed following the instructions of the Q5 site-directed mutagenesis kit (NEB) (primers 35-38 ...
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bioRxiv - Microbiology 2019Quote: ... The reacted DNA was then purified using PCR clean-up kit (New England Biolabs®) and quantified using the nanodrop ...
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bioRxiv - Microbiology 2019Quote: ... sgRNA sequences were cloned using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs). Primers were designed using the NEBaseChanger™ tool (v.1.2.9) ...
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bioRxiv - Microbiology 2020Quote: ... we introduced the desired mutations using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) into pfixK.21 pfixK contains the AU0158 fixLJ sequence along with 670 bp upstream within the pUC18-mini-Tn7-Tp back bone allowing for stable chromosomally integration at an attTn7 site.23,50 Point mutations were verified by Sanger sequencing ...
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bioRxiv - Cancer Biology 2020Quote: ... Libraries were prepared using the NEBNext Ultra Directional RNA Library prep kit (NEB cat # E7420S). Libraries were then sequenced on the Illumina NextSEQ500 ...
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bioRxiv - Cancer Biology 2020Quote: ... Libraries were prepared using the NEBNext Ultra II DNA Library prep kit (NEB cat # E7645). 3 cycles of PCR amplification were used for native inputs ...
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bioRxiv - Cancer Biology 2020Quote: ... with 500ng input RNA or Ultra RNA Library Prep Kit for Illumina (New England Biolabs). An Illumina HiSeq 2500 was utilized to performed paired-end sequencing.
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bioRxiv - Cancer Biology 2020Quote: ... were generated using NEBNext Single Cell/Low Input RNA Library Preparation kit (New England Biolabs) and sequenced in a NextSeq500 sequencer SE75bp (Illumina) ...
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bioRxiv - Cell Biology 2021Quote: ... was mutated using the Q5 site directed mutagenesis kit (New England Biolabs, catalog number E0554S) and designed primers according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Point mutations were introduced using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs). For fluorescence microscopy ...
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bioRxiv - Cell Biology 2021Quote: ... and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760L, New England Biolabs) with in-house adapters and barcode tags (using dual indexing ...
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bioRxiv - Bioengineering 2022Quote: cDNA was synthesized from RNA using the Protoscript II First Strand cDNA Synthesis Kit (NEB). qPCR was performed using a CFX Connect Real Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Biochemistry 2022Quote: ... The C85S mutation was produced using a Q5 Site Directed Mutagenesis Kit (New England Biolabs) and standard cloning techniques ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were PCR purified using Monarch® PCR DNA Cleanup Kit (New England BioLabs) and eluted with water ...
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bioRxiv - Cell Biology 2022Quote: ... and cDNA was synthesized with ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs) using 1 µg RNA ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by 9 cycles of PCR enrichment using NEB High fidelity PCR kit (NEB, M0541S). The quality of ATAC libraries was assessed with Agilent Bioanalyzer with DNA High Sensitivity kit (Agilent Technologies ...
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bioRxiv - Plant Biology 2022Quote: ... and gl2E294Q were generated using the Q5 Site–Directed Mutagenesis Kit (E0554S, New England Biolabs) following manufacturer’s protocol with primers listed in Supplemental Table 3 ...
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bioRxiv - Molecular Biology 2022Quote: ... Eluted RNA was used for library preparation using the NEBNext small RNA library kit (NEB). The enriched and control libraries were amplified through 16 cycles and 10 cycles of PCR ...
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bioRxiv - Cell Biology 2022Quote: ... R256I and F257I were made by Q5 site-directed mutagenesis kit (New England BioLabs, E0554S) with base-pair substitutions according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2022Quote: RNA synthesis was performed using the HiScribe T7 High Yield RNA synthesis kit (NEB #E2040S) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... The deletion mutants were generated using the Q5 site-directed mutagenesis kit (New England Biolabs) and phospho-ablative mutants were constructed by Gibson assembly of the corresponding mutant gene fragments (IDT) ...
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bioRxiv - Molecular Biology 2022Quote: ... Pre-adenylation was performed on 5 nmoles using the 5’ DNA Adenylation Kit (NEB, E2610L) as follows ...
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bioRxiv - Genetics 2022Quote: ... The cut vector was gel purified using the Monarch Gel Purification kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... library preparation was conducted using a NEBNext Ultra RNA Library Prep kit for Illumina (NEB). After cluster generation ...
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bioRxiv - Cancer Biology 2022Quote: ... after which DNA was acquired using the Monarch DNA cleanup kit (NEB, Ipswich, MA, USA), and DNA-based qPCR quantification was done ...
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bioRxiv - Molecular Biology 2022Quote: ... and purification of the PCR fragments was done using the Monarch Gel Purification Kit (NEB). All fragments for plasmids cloning were amplified using Q5 DNA Polymerase (NEB ...
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bioRxiv - Neuroscience 2022Quote: ... Sequencing libraries were prepared using the Next Ultra RNA Library Prep kit (New England Biolabs) with 12 cycles of PCR amplification ...
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bioRxiv - Plant Biology 2022Quote: ... and subsequently introduced into pDC45_dsg vector using Golden Gate Assembly Kit (E1601L, New England Biolabs). The tobacco FT gene was amplified using NtFT-F and NtFT-R primers ...
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bioRxiv - Molecular Biology 2022Quote: RNA from K562 cell lines was extracted using the Monarch Total RNA purification kit (NEB) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... Sequencing libraries were prepared using NebNext Ultra II DNA Library Prep Kit (New England BioLabs) with Methylated Adaptor (New England BioLabs) ...
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bioRxiv - Genomics 2022Quote: ... IVT was performed to generate dsRNA using the T7 High Yield RNA Synthesis Kit (NEB). Template DNA was removed using Turbo DNAse (Ambion ...
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bioRxiv - Microbiology 2022Quote: ... The Monarch DNA Gel Extraction and Monarch PCR and DNA Cleanup kits (New England Biolabs) were used to clean PCR products according to manufacturers instructions ...
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bioRxiv - Biophysics 2022Quote: ... Libraries were quantified with quantitative PCR (NEBnext Library quantification kit, New England Biolabs, Ipswich, USA) and an Agilent Bioanalyzer High Sensitivity DNA chip ...
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bioRxiv - Bioengineering 2022Quote: ... The PCR product was purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs, Inc.). The samples were run on a 1.5% Agarose (Merck ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were run using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) on a CFX384 (Bio-Rad).
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bioRxiv - Microbiology 2022Quote: ... RNA samples were prepared using the Monarch Total RNA Miniprep kit (New England Biolabs Inc.), followed by a DNAse treatment using the turbo DNA-free kit (Life Technologies) ...
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bioRxiv - Cell Biology 2022Quote: ... Reagents and general procedures from the Gibson Assembly Cloning Kit from New England Biolabs (NEB) were used ...
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bioRxiv - Plant Biology 2021Quote: ... Sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) and sequenced on an Illumina Hiseq Xten platform ...
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bioRxiv - Neuroscience 2020Quote: RNA was reverse transcribed using LunaScript RT SuperMix Kit (New England Biolabs, Ipswich, MA, USA). To confirm that striatal ChIs or midbrain dopaminergic neurons were collected via LCM ...
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bioRxiv - Microbiology 2020Quote: ... Sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Microbiology 2020Quote: ... the sequencing library was prepared using NEBNext Ultra II Directional RNA Library Prep Kit (NEB), and sequenced with a paired-end 150 bp run on an Illumina NextSeq ...
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bioRxiv - Microbiology 2021Quote: ... libraries were prepared using the Ultra DNA Library Prep kit for Illumina (New England Biolabs). Additional steps of clean-up were performed using AMPure XP beads ...
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bioRxiv - Microbiology 2021Quote: ... The libraries were quantified using NEB Next Libraries Quantification kit for Illumina (New England Biolabs) and multiplexed at equimolarity ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... CA using NEBNext Ultra II RNA Library Prep Kit for Illumina (New England BioLabs, #E7775) according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... RNA was isolated and purified using the total RNA extraction and purification kit (Monarch, NEB). RNA concentration and integrity were calculated using a Nanodrop and agarose gel electrophoresis ...