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Citations for New England Biolabs :
3301 - 3350 of 4333 citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... 10 or 20 minutes at 37°C and immediately quenched with 8μL 6x NEB purple loading dye (NEB B7024S) supplemented with 6mM EDTA (to make the total EDTA concentration in the stock tube 66mM) ...
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bioRxiv - Biochemistry 2022Quote: ... were assembled using 10 μl of 2× master mix at 50 °C for 1 h according to manufacturer’s instructions (NEB). 5α Competent Escherichia coli (30 μl ...
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bioRxiv - Microbiology 2022Quote: ... 30 nM EnGen LbCas12a was pre-incubated with 30 nM crRNA in NEBuffer 2.1 for 10 minutes at 25°C. Substrate DNA (i.e. dsDNA template, M13mp18 ssDNA (NEB #N4040S) or purified Pf4 DNA ...
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bioRxiv - Physiology 2022Quote: ... A 20 min A-tailing step (72 °C, 0.2 mM dATP) was performed with taq polymerase (NEB, Ipswich, USA).
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bioRxiv - Microbiology 2022Quote: ... This was then incubated at 37 °C for 1.5 h with 50 μL of 10× CutSmart Buffer (New England BioLabs) supplemented with 5 mM CaCl2 ...
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bioRxiv - Molecular Biology 2022Quote: ... 404–561 with a C terminal His tag added was cloned into the pMAL-c5x vector (New England Biolabs), and were transformed in E ...
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bioRxiv - Microbiology 2022Quote: ... 1 μg of genomic DNA was digested with the restriction enzyme AatII overnight at 37°C to generate fragments of genomic DNA which were on average 1,500 bp (New England Biolabs). The digestion product was then treated with Antarctic phosphatase for 1 hour at 37°C (New England Biolabs) ...
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bioRxiv - Microbiology 2022Quote: ... pCHYC-1 and a 500 bp ‘hfaE C-terminal PCR product were digested with HindIII-HF and KpnI (NEB) and ligated with T4 DNA ligase (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were incubated at 37°C for 1 h after the addition of T4 PNK (New England Biolabs M0201) to allow 5’-hydroxyl repair ...
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bioRxiv - Microbiology 2023Quote: ... An aliquot of 10 μg DNA was digested overnight at 37°C using NlaIII and MseI restriction enzymes (NEB), purified using Sera Mag Speedbeads (Thermo Scientific) ...
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bioRxiv - Biophysics 2022Quote: ... The C-terminal Flag tag was introduced into pcDNA3.1-Spike-WT thanks to NEBuilder® HiFi DNA Assembly (NEB). The Flag tag was added during amplification of Spike-WT from pcDNA3.1-Spike-WT with the primers ...
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bioRxiv - Genomics 2023Quote: ... The reaction was inactivated at 25 °C for 10 min with 1.25 μL of Murine RNAse inhibitor (New England Biolabs).
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bioRxiv - Biochemistry 2023Quote: ... 0.1 mM EDTA) and digested with DraIII-HF in 1x CutSmart buffer for ∼20 hr at 37 °C (NEB). Fragments with DraIII sticky ends were re-purified by 1 mL mono-Q ion-exchange ...
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bioRxiv - Cell Biology 2023Quote: ... APOE3-C-mEm ΔSS were created from APOE3-mEm using Q5 Site-Directed Mutagenesis Kit (New England Biolabs, E0554S). The N-terminal domain constructs consist of residues 19-209 with or without residues 1-18 of the N-terminal signal peptide ...
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bioRxiv - Plant Biology 2024Quote: ... and SLAC1 cDNAs (N-terminal or C-terminal) were cloned in-frame into pMAL-c5X vector (New England Biolabs) using In-Fusion HD Cloning Kit (Takara Bio) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... were split into two equal samples and incubated at 50°C for 25 minutes with exonuclease 1 (NEB, M0293) to remove unintegrated barcoded transposon adapters ...
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bioRxiv - Cell Biology 2024Quote: ... Ligation reactions were quenched by addition of EDTA to 25 mM and proteins were digested by incubation at 37°C for 20 min with 0.1% SDS and 20 units/ml proteinase K (NEB). Unligated oligonucleotides were removed by gel filtration through a 50 cm x 0.7 cm Sepharose 4B column (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... treated or not for one hour at 56°C with 0.4 mg/mL proteinase K (P8107S, New England Biolabs) and then heated or not for one hour at 85°C.
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bioRxiv - Cell Biology 2024Quote: Purified or crude viral supernatant was DNase I treated for 90 minutes at 37°C according to manufacturer’s instructions (New England BioLabs). qPCR was performed using a custom FAM probe against Nano Luciferase (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA synthesis was performed in 20 µl at 42°C for 1 h using AMV reverse transcriptase (NEB, M0277), and 5 µl of the RT reaction was amplified in 25 µl using AccuPrime Pfx DNA polymerase (ThermoFisher ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA synthesis was performed in 20 µl at 42°C for 1 h using AMV reverse transcriptase (NEB, M0277), and 5 µl of the RT reaction was amplified in 25 µl using AccuPrime Pfx DNA polymerase (ThermoFisher ...
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bioRxiv - Molecular Biology 2024Quote: ... plus 100 µg/mL BSA) and A-tailed (30 min 37°C; NEBNext® dA-Tailing Module; NEB, E6053L) with intervening CutsmartTM washes ...
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bioRxiv - Microbiology 2024Quote: ... Ligation reactions were carried out overnight at 16°C using 1µl of T4 DNA Ligase (20 U/µl; NEB). Plasmids were then cloned in E ...
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bioRxiv - Genomics 2024Quote: ... the RT product was digested overnight at 37°C with 1 unit of USER Enzyme (New England Biolabs, M5505L) per µg of product ...
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bioRxiv - Molecular Biology 2024Quote: ... 87 µL of CAD was activated by addition of 10µL of TEV buffer and 3µL of TEV enzyme for 1hr at 30°C (NEB, P8112S).
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bioRxiv - Molecular Biology 2024Quote: ... proximity ligation was carried out overnight at 16°C with 10000U of T4 DNA Ligase (New England Biolabs, #M0202M). Chromatin was reverse-crosslinked with 16U of Proteinase K (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... primers TZ-54 and TZ-55 were used to amplify the C-terminus of CapRelSJ46 using Taq polymerase (NEB) and 0.5 mM MnCl2 was added to the reaction as the mutagenic agent ...
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bioRxiv - Neuroscience 2024Quote: ... pH 8.0) for 1 h at 37 °C and washes in CSTX buffer (1× CutSmart buffer (New England Biolabs (NEB) B60004) ...
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bioRxiv - Molecular Biology 2024Quote: The C-type CA sequence was cloned into pCMVHTLV_delta env (kind gift from Dimitry Mazurov) by Gibson Assembly (NEB) and all subsequent point mutations were introduced by standard overlapping PCR ...
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bioRxiv - Immunology 2024Quote: ... The ligation reaction was performed at 16 °C for 16 hours using 2.5 units of T4 DNA ligase (M0202, New England Biolabs). Subsequently ...
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bioRxiv - Biochemistry 2024Quote: The purified nanobody DNA fragments and linearized vector were ligated overnight at 16°C using T4 DNA ligase (NEB). The ligation products were cleaned using a QIAquick PCR Purification Kit (Qiagen ...
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bioRxiv - Biochemistry 2024Quote: ... Methylation proceeded for 30 minutes at 37°C before adding 10µL of 10% SDS 2.5µL ProK (NEB, 800U/mL). Proteinase K digestion proceeded for 2 hours at 65°C ...
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bioRxiv - Genomics 2024Quote: ... the PCR product was digested overnight at 37°C with 1 unit of NheI-HF (New England Biolabs, R3131) per µg of product ...
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bioRxiv - Genomics 2024Quote: ... a 16 hour RCA reaction was performed at 30°C using a mixture containing 500 µM dNTP (NEB #N0447S), 50 µM amino-allyl-dUTP (Thermo Scientific #R1091) ...
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bioRxiv - Genomics 2024Quote: ... 10 mM DTT and 300 mM sucrose) and incubated for 7.5 minutes at 37°C with 200U of M.CviPI (NEB M0227L) and 0.6 mM SAM (NEB) ...
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bioRxiv - Cancer Biology 2024Quote: ... The WI290>C substitution was generated in the pMSCV-FGFR2wt construct using the Q5 site-directed mutagenesis kit (NEB). pMSCV-Blasticidin (empty vector ...
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bioRxiv - Biochemistry 2024Quote: ... The eluted protein:RNA complex was then de-crosslinked overnight at 65°C with constant shaking at 1000 rpm in the presence of 2.7 U/ml proteinase K (NEB) to digest the proteins ...
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bioRxiv - Biophysics 2021Quote: ... 1 μL of this was then combined with 5 μL Q5 buffer (NEB, cat#M0491 S), 0.5 μL 10mM DNTP (Thermo Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... 10ul of PCR product was digested with 5 units of Msel (New England Biolabs, Ipswich, Massachusetts) for one hour ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Then every 5 μL of cleaned eluted product was redigested with 5μL of NotI-HF (NEB), 5 μL 10X CutSmart buffer (NEB) ...
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bioRxiv - Developmental Biology 2022Quote: ... 5µl of the PCR mix was digested overnight with 5 units of BbsI (New England Biolabs) in 30 µl volume ...
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bioRxiv - Molecular Biology 2021Quote: ... The 5’-end of dephosphorylated mRNAs were then labeled using 10 units of T4 PNK (NEB) and 0.5 µl [γ-32P] ATP (6000 Ci/mmol ...
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bioRxiv - Molecular Biology 2020Quote: ... and 5’ adaptor (rGrUrUrCrArGrArGrUrUrCrUrArCrArGrUrCrCr-GrArCrGrArUrCrNrNrNrCrGrArNrNrNrUrArCrNrNrN, N is for a random nucleotide) by T4 ligase 1(NEB) sequentially ...
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bioRxiv - Molecular Biology 2020Quote: ... and 5 μL of NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, Ipswich, Massachusetts, USA). Finally ...
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bioRxiv - Genomics 2020Quote: ... 5mM DTT) and phosphorylated the 5’-ends with 25 units of T4 Polynucleotide Kinase (NEB #M0201) at 37°C for 15 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... This was added to 5 ml (per 2 L of culture) amylose resin (New England Biolabs) equilibrated in lysis buffer and left on a tube roller shaker at 4 °C for 1 h ...
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bioRxiv - Cancer Biology 2021Quote: ... 5-fold molar access of pre-annealed T7 promoter adapters and either 80 units HindIII (NEB) or 40 units SpeI (NEB ...
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bioRxiv - Microbiology 2020Quote: ... We incubated 10 pmol gRNA with 5 pmol SauCas9 (New England Biolabs [NEB], Ipswich, MA, USA) at 25 °C for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... We incubated 10 pmol gRNA with 5 pmol SauCas9 (New England Biolabs [NEB], Ipswich, MA, USA) at 25 °C for 10 min ...
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bioRxiv - Genomics 2020Quote: ... each eluted insert was mixed with 50ng pDXinit-PAC in a molar ratio of 1:5 (vector:insert) in 10μl reactions and digested with 0.5μl SapI (NEB) for 1h at 37°C ...