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Citations for New England Biolabs :
3101 - 3150 of 4333 citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... RNAs were 5’dephosporylated through 90 minutes incubation in total with thermostable QuickCIP (NEB) in which the samples were briefly heated to 75°C and quickly chilled on ice at the 60 minutes mark ...
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bioRxiv - Immunology 2023Quote: ... 5 µg of sequencing verified plasmid were restriction digested using BsmBI V2 (NEB, #R0739S) in a 50 µl reaction with NEB3.1 buffer (NEB ...
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bioRxiv - Microbiology 2024Quote: 5’ RACE was done using a Template Switching (TS) Reverse Transcriptase Enzyme mix (NEB) that takes advantage of a template switching reverse transcriptase and a template switching oligonucleotide (TSO) ...
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bioRxiv - Genomics 2024Quote: ... RNAs were 5’dephosporylated through 90 minutes incubation in total with thermostable QuickCIP (NEB) in which the samples were briefly heated to 75°C and quickly chilled on ice at the 60 minutes mark ...
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bioRxiv - Genetics 2024Quote: ... along with an additional 5 µL 2x Gibson Assembly Master Mix (NEB Cat#E2611L). The reaction mixture was incubated at 50°C for 1 hour ...
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bioRxiv - Molecular Biology 2024Quote: ... coli 5-alpha and purified using the Monarch Plasmid Miniprep Kit (New England Biolabs).
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bioRxiv - Molecular Biology 2024Quote: ... and fragment inserts were cloned into digested pLS21-5 using Gibson assembly (NEB, E5510S). The plasmid pARL01 ...
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bioRxiv - Neuroscience 2024Quote: ... Each PCR reaction was subsequently supplemented with 5 μL of CutSmart Buffer (NEB B7204) and digested using 1μL DpnI restriction enzyme (NEB R0176 ...
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bioRxiv - Neuroscience 2024Quote: ... 0.5 uL of 5 x mRNA Second Strand Synthesis buffer (New England Biolabs, E6111L), and 0.25 uL of mRNA Second Strand Synthesis enzyme (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... with 5 μL Quick-Load pBR322 DNA-MspI Molecular Marker (New England Biolabs Inc.) in a separate lane ...
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bioRxiv - Biochemistry 2024Quote: ... The assembly reaction was transformed into NEB 5-alpha competent cells (New England Biolabs). After the recovery step ...
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bioRxiv - Molecular Biology 2024Quote: ... Coverslips were then washed twice for 5 min in 1x rCutSmart buffer (NEB; #B6004S) and once in 1x blunting buffer (NEB ...
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bioRxiv - Genetics 2024Quote: ... Dangling ends were removed by a 5 min incubation with Exonuclease III (NEB #0206) at 37 °C and biotin enrichment was done using 20 ul DynabeadsTM MyOneTM Streptavidin C1 beads (Invitrogen #65001) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Constructed plasmids were transformed and maintained in NEB 5-alpha (New England Biolabs (NEB) C2987H ...
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bioRxiv - Synthetic Biology 2024Quote: ... Constructed plasmids were transformed and maintained in NEB 5-alpha (New England Biolabs (NEB) C2987H ...
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bioRxiv - Cell Biology 2024Quote: ... 5-10 μg of plasmid was linearised with the restriction enzyme NotI (NEB, R3189L). The linearised plasmids were run on a gel to ensure complete digestion ...
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bioRxiv - Synthetic Biology 2024Quote: ... Transformations used the NEB recommended protocols for chemical transformation (NEB 5-alpha, Cat# C2987H) or electroporation (NEB 10-beta ...
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bioRxiv - Plant Biology 2024Quote: ... for 30 minutes and the supernatant mixed with 5 mL Chitin Resin (NEB #S6651), previously washed first with 30 mL of ddH2O and then 20 mL of HEGX buffer ...
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bioRxiv - Genomics 2024Quote: ... we added 5 pg of unmethylated DNA from phage λ (New England Biolabs, E7123A) per 10 ng sample DNA ...
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bioRxiv - Bioengineering 2024Quote: ... digested vec-tors were treated with 5 units of Antarctic Phosphatase (New England Biolabs) for 30 min at 37°C ...
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bioRxiv - Biophysics 2024Quote: ... The purified plasmid DNA (5 µg) was incubated with T4 DNA ligase (NEB, M0202) (2.5 µL ...
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bioRxiv - Genetics 2024Quote: ... 5 μL NEB T4 DNA Ligase from the NEB Quick Ligation Module (NEB, E6056), and 1.0 μL Native Adapter (NA ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We then immediately added 5 µL of a solution with 1X Ligase Buffer (NEB), 0.75 mM ATP (NEB) ...
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bioRxiv - Genetics 2021Quote: ... The entire alh-6 genomic sequence (ATG to stop) was amplified by PCR and cloned in a linearized pMiniT 2.0 vector (NEB PCR Cloning Kit, Cat. #E1202S). Plasmid DNA was purified using the Zymo Research Zyppy Plasmid Miniprep kit (Cat ...
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bioRxiv - Microbiology 2020Quote: ... The ClbI C-terminal GFP fusion was constructed using the Gibson Assembly kit (New England Biolabs, MA, USA). Briefly ...
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bioRxiv - Genetics 2021Quote: ... A rolling circle amplification reaction (RCA) of partially double-stranded C-circles was performed with 0.2μg/µl bovine serum albumin (NEB), 0.1% Tween-20 (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: ... Two 100 μl aliquots were incubated overnight at 37°C with and without Endo H (3000 U, NEB). Then the samples were heated with 100 μl SDS sample buffer for 5 min at 95°C.
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bioRxiv - Bioengineering 2021Quote: ... H2-Db and H2-Kb genes were engineered with a C-terminal cysteine by site-directed mutagenesis (NEB), and pMHC molecules were expressed and refolded as described previously[40] ...
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bioRxiv - Neuroscience 2020Quote: ... Vector and insert were digested (30 min, 37 °C) according to manufacturer’s protocols (enzymes by New England Biolabs), either purified directly (insert ...
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bioRxiv - Biophysics 2020Quote: ... followed by treatment for 1 h at 37 °C with 2.5 units of RNase-free DNase I (NEB). The sample was once again cleaned with RNeasy MinElute Cleanup Kit before applying on a 1% agarose gel for gel extraction and purification with QIAGEN gel extraction kit and elution with RNase free H2O ...
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bioRxiv - Microbiology 2020Quote: ... followed by 10 cycles of annealing at 54°C for 30 s) using Taq polymerase (New England Biolabs) according to the manufacturer’s instructions and the primers 5-CTTGACATCTCTGAGGCAAC-3 and 5-ATGCAGGGGCAAAGTCATTAG-3 ...
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bioRxiv - Microbiology 2021Quote: ... 100ng of vector was digested for 1 hour at 37°C with 10 U of BamHI-HF (NEB) to create a single double-stranded cut or 10 U Nb.BtsI (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... The E PBM C-terminal mutation (DVLL>GGGG) was performed using the Q5 site-directed mutagenesis kit (NEB). The sequences corresponding to the PDZ domains were cloned into the pCMV GFP vector using EcoRI and XhoI sites.
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bioRxiv - Molecular Biology 2020Quote: ... Around 500ng of RNA were ligated overnight at 16°C using 20units of T4 RNA ligase 1 (NEB) in the presence of 10% PEG 8000 to favour intramolecular ligations ...
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bioRxiv - Molecular Biology 2020Quote: ... Around 500ng of RNA were ligated overnight at 16°C using 20units of T4 RNA ligase 1 (NEB) in the presence of 10% PEG 8000 to favour intramolecular ligations ...
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bioRxiv - Biophysics 2020Quote: 20μg of pRBD vector was linearized with the PmeI enzyme by incubating at 37°C overnight (NEB, R0560). Enzyme was inactivated (65°C ...
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bioRxiv - Biochemistry 2020Quote: ... The transcription reaction was carried out at 37°C for 12 hours with T7 polymerase (New England Biolabs) in the presence of 13C/15N labeled nucleotide triphosphates (Cambridge Isotope Laboratories ...
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bioRxiv - Bioengineering 2021Quote: ... H2-Db and H2-Kb genes were engineered with a C-terminal cysteine by site-directed mutagenesis (NEB), and pMHC molecules were expressed and refolded as described previously (19).
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bioRxiv - Evolutionary Biology 2022Quote: ... The reaction was incubated for 1 hour at 37 °C and stopped by adding 6X loading dye (NEB) with EDTA and run 2% agarose gel ...
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bioRxiv - Genetics 2020Quote: ... Pairs of annealed oligos and the digested vector were ligated overnight at 16°C using T4 ligase (NEB). Following transformation ...
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bioRxiv - Biochemistry 2021Quote: ... also C-terminal) FT MYOC variants were generated by DNA assembly (NEBuilder HiFi DNA Assembly Cloning Kit, NEB) by combining a PCR-amplified eGLuc2 product from a pcDNA3 MYOC eGLuc2 plasmid (originally described previously8) ...
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bioRxiv - Biochemistry 2022Quote: ... The reaction was incubated at 37°C overnight and stopped by adding 2 Units of DNase I (NEB) and incubating at 37°C for 15 minutes ...
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bioRxiv - Microbiology 2022Quote: ... The digestion product was then treated with Antarctic phosphatase for 1 hour at 37°C (New England Biolabs). PCR products were purified using the Zymogen Clean and Concentrator PCR-cleanup kit (Zymo Research ...
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bioRxiv - Microbiology 2022Quote: ... Each mixture was incubated at 50°C for 30 minutes after which 0.6μL (3U) of MboI (NEB R0147) was added to each reaction ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were fragmented at 94°C for 2.75 min as per the NEBNext Magnesium RNA Fragmentation Module (NEB) with subsequent RNA purification using the Zymo RNA Clean & Concentrator kit ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated with primary antibody rabbit anti-c-Fos (1:3000, New England Biolabs cat. No. 2250S) for 24 hours at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Ligations into the respective backbone were performed overnight at 16°C using T4 DNA Ligase Kit (NEB, #M0202L). Ligations were transformed into NEB Stable E ...
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant was digested for 1 h at 37°C with PNGase F (New England Biolabs, P0704) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg of DNA was incubated 20min at 37°C with 15 units of RNAseH1 (New England Biolabs) and first digested for 2h at 37°C with 35 units of AsiSI restriction enzyme (New England Biolabs) ...
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bioRxiv - Microbiology 2022Quote: ... Inserts were ligated into the linearized plasmid overnight at 16°C using T4 DNA ligase (New England Biolabs) following the manufacturer’s guidelines ...