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Citations for New England Biolabs :
2951 - 3000 of 3340 citations for 7 Decyn 1 ol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... was resuspended in 10x NEB CutSmart Buffer (8:1) and dephosphorylated by incubation with Quick calf intestinal phosphatase (CIP) (NEB, # M0525S) at 37 °C for 20 min ...
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bioRxiv - Molecular Biology 2021Quote: ... then incubated for 2 h at 37°C in 100 μl 1x TdT buffer containing 4 μl 10 mM ATP and 1 μl Terminal Transferase (NEB M0315L). Plugs were rinsed with 1 ml tris buffer (10 mM Tris HCl pH 8.0) ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μl of 10 mM dATP and 3 μl of 5 U/μl of Klenow fragment (3′→ 5′ exo (-)) (NEB, M0212) were added and the sample was incubated for 30 min at 37 °C followed by a deactivation step at 65 °C for 20 min ...
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bioRxiv - Molecular Biology 2021Quote: TCF20(1-1268)-mEGFP construct was generated from the TCF20-mEGFP plasmid using the Q5 Side-Directed Mutagenesis Kit (NEB, E0554S) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Typhimurium NCTC 12023 and the vector including the Tet-on system aph tetR PtetA present on p4392 occurred using oligonucleotides as listed in Table S 1 and purified by PCR purification (NEB Monarch). The PCR product encoding for sadBA and the PCR product from vector p4392 were assembled by Gibson assembly according to manufactureŕs protocol (NEB Monarch) ...
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bioRxiv - Biochemistry 2020Quote: ... The CIP-treated RNA was then PNK-treated with 1 μL of 10× T4 polynucleotide kinase buffer (New England Biolabs, B0201S), 2 μL of [γ-P32]ATP (PerkinElmer ...
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bioRxiv - Microbiology 2019Quote: ... All fragments contained ~20bp overhangs and were assembled into EcoRV cut pLenti CMV Puro DEST (w118-1) using NEBuilder® HiFi DNA Assembly Master Mix (New England BioLabs) or Gibson-like master mix (Berkeley MacroLabs ...
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bioRxiv - Genomics 2019Quote: ... The amount of DNA was estimated by running an aliquot on a 1% Agarose gel along with a 1kb ladder (NEB#N3232s).
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bioRxiv - Microbiology 2019Quote: ... These fragments were cloned into the XhoI site of pACYCDuet-1 by a NEBuilder HiFi DNA Assembly kit (New England Biolabs, USA), resulting in the plasmid pEXT06 ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were diluted to 0.1 OD595 in 1 mL of EMM5S containing 1 µM of silicon-rhodamine benzylguanine derivative SNAP-SiR647 (SNAP-Cell® 647-SiR, New England Biolabs). Culture tubes were wrapped in aluminum foil to protect them from light and incubated on a rotator overnight (about 15 hours ...
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bioRxiv - Microbiology 2019Quote: ... Eluted DNA was transferred to a new tube and PCR enrichment of the adaptor ligated DNA was performed using NEBNext Multiplex Oligos for Illumina (Set 1, NEB #E7335). The PCR reaction mix was assembled according to manufacturers’ recommendations and placed in a thermal cycler set to the recommended conditions ...
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bioRxiv - Synthetic Biology 2019Quote: Fly genomic DNA was isolated in a pool by grinding in 25 µl of “Squish Buffer” (10 mM Tris, 1 mM EDTA, 25 mM NaCl, 8 U/ml ProK (NEB P8107S)) per adult ...
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bioRxiv - Genetics 2021Quote: ... barcodes and barcoding adapters (PCR Barcoding Expansion 1-96, EXP-PBC096, Oxford Nanopore Technologies, UK) by PCR using Q5 polymerase mastermix (NEB, USA) for individual fish identification according to manufacturer’s protocol in a 25μl reaction (98°C for 3 min ...
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bioRxiv - Genomics 2021Quote: ... 0.5 µg of the treated RNA was used for ligation to an RNA oligonucleotide with T4 RNA ligase 1 (NEB M0204) for 1 hr at 25 °C then converted to first strand cDNA with random priming and the ProtoScript II reverse transcriptase mix (NEB E6560) ...
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bioRxiv - Genomics 2021Quote: ... and a portion corresponding to 1 µg input RNA was converted to cDNA using the LunaScript RT SuperMix cDNA synthesis kit (NEB E3010). 1% of the cDNA was used for each qPCR reaction performed with the Luna Universal qPCR Mastermix (NEB M3003 ...
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bioRxiv - Developmental Biology 2020Quote: ... The samples were analyzed by SDS-PAGE and western blotting by using antibodies against MBP (1:1000, New England Biolabs, E8038S) or GST (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... fragments were generated by PCR from the indicated template with the indicated primers (Data File 1) and assembled using NEBuilder HiFi DNA Assembly (NEB E5520S). Plasmids were transformed into NEB competent cells (NEB C2987I) ...
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bioRxiv - Genomics 2021Quote: ... 2 μg genomic DNA from mESCs (both, with DSB induction and control without the induction) were digested using 1 μl 100 U/μL XbaI (NEB, #R0145T) and 4 μl 5 U/μL I-SceI (NEB ...
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bioRxiv - Cell Biology 2021Quote: ... 50 mM Tris-Cl pH 7.4, 12 mM MgCl2, 1% NP-40, 0.5 mM DTT, 0.1 mg/ml cycloheximide, 80 U/ml NEB murine RNase inhibitor). Beads were transferred to a fresh tube and immunoprecipitated RNA extracted by incubating beads in buffer RLT (Qiagen ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting fragment was then checked by 1% agarose gel and purified with the Monarch® DNA Gel Extraction Kit (New England Biolabs). The purified fragment was precipitated by ethanol precipitation and resuspended in buffer (25 mM Tris ...
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bioRxiv - Bioengineering 2021Quote: ... qRT-PCR was performed with primers specific for target genes (see Supplementary Table 1 for the list of primers) using the Luna universal One-Step RT-qPCR kit (NEB; E3005). Experiment was performed using the QuantStudio3 (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... a colony of each isolate was resuspended in 50 µL of sterile water then 1 µL of resuspended bacteria was used in a PCR reaction using Phusion® High-Fidelity DNA Polymerase (New England Biolabs) with forward (16s-8F ...
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bioRxiv - Systems Biology 2020Quote: ... the samples were then incubated with 20-fold diluted Quick Ligase in 1× Quick Ligase Reaction Buffer from Quick Ligation Kit (NEB M2200) supplemented with additional 1 mM ATP (NEB P0756 ...
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bioRxiv - Systems Biology 2020Quote: ... 10 mM DTT, 12% PEG 8000, 1 mM each dNTPs, 10 µM dN-SMRT oligo, 5 µl Klenow enzyme NEB #M0212) for 1 h at 37 °C ...
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bioRxiv - Systems Biology 2021Quote: ... The samples were then incubated with 20-fold diluted Quick Ligase in 1× Quick Ligase Reaction Buffer from Quick Ligation Kit (NEB M2200) supplemented with an additional 1 mM ATP (NEB P0756 ...
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bioRxiv - Microbiology 2021Quote: ... and then subcloned into the directly downstream of PreScission protease site pGEX-6P-1 (Cytiva) by Gibson Assembly system (New England Biolabs Inc.) to create pRN108 ...
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bioRxiv - Microbiology 2021Quote: ... was ligated with the digested vector in a ratio of 3:1 using the Gibson assembly ligation matrix mix (NEB E5510S). Each ligation reaction was incubated for 1 hour at 50 °C ...
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bioRxiv - Microbiology 2020Quote: ... A band at approximately 1 kb was cut out and DNA extracted using the Monarch DNA Gel Extraction Kit (New England Biolabs, US) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the sgRNA cassette from lentiCRISPRv2 was removed by digestion with KpnI and EcoRI and the plasmid re-ligated using annealed repair oligo pair 1 (see Table S3) and Quick Ligation™ Kit (M2200L, NEB). Next ...
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bioRxiv - Plant Biology 2021Quote: ... deltoides xylem cDNA library (primers listed in Supplemental file 1) using Q5 High-Fidelity DNA polymerase (New England Biolabs, Ipswich, MA) and cloned in a pENTR vector (Invitrogen ...
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bioRxiv - Zoology 2021Quote: ... amplicons were analyzed by 1.5% agarose gel electrophoresis with ethidium bromide staining and using a DNA ladder marker (2 log, 100 bp, or 1 kb DNA ladder from New England Biolabs, USA). Expected PCR product sizes of the first step and nested PCR step were 514 and 148 bp ...
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bioRxiv - Molecular Biology 2021Quote: ... and subsequently purified by extraction from a 1% agarose gel using the Monarch DNA Gel Extraction Kit following manufacturer instructions (NEB # T1020S). The partially degenerate DNA oligonucleotide ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 µg of GST-beads were incubated for 15 min on ice in GRB buffer in a 25 μl reaction volume with 1 µg of 100 bp DNA ladder (New England Biolabs # N3231S) or mononucleosome (Active motif # 81070) ...
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bioRxiv - Microbiology 2019Quote: ... nearly 1 kb upstream and downstream sequences flanking the bycA gene were PCR amplified (Phusion® High-Fidelity DNA Polymerase, NEB) using primers SL3 and SL4 ...
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bioRxiv - Molecular Biology 2022Quote: ... Purified inserts and expression vectors pGEX-4T 1 and pMALTMc5X were digested with BamHI/SalI (for pGEX-4T 1 constructs) and NotI/PstI (for pMALTMc5X constructs) restriction enzymes (New England Biolabs, UK) and were ligated over night at 16 °C using T4 DNA ligase (New England Biolabs ...
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An alternatively spliced TREM2 isoform lacking the ligand binding domain is expressed in human brainbioRxiv - Molecular Biology 2022Quote: The cDNA samples from the anterior cingulate samples were amplified using primers corresponding to TREM2 exon 1 (5’-CCTGACATGCCTGATCCTCT-3’) and exon 5 (5’-GTGTTCTTACCACCTCCCC-3’) with Q5 high-fidelity hot-start polymerase (NEB # M0493L). Thermocylcing parameters were as follows ...
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bioRxiv - Synthetic Biology 2022Quote: Templates for expression of MGVDYKDDDDK were prepared by annealing and extending the oligonucleotides MGVflag-1 and MGVflag-2 using Q5® High-Fidelity 2X Master Mix (NEB) (Supplementary Table 1) ...
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bioRxiv - Genomics 2022Quote: ... Biotin-labeled DNA fragments captured on beads were ligated to multiplexing adaptors by adding 5 μL each adapter (50 μM) and 1 μL T7 DNA ligase (NEB, M0318L) in 100 μL ligation mixture and incubating at room temperature for 1 hour with rotation ...
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bioRxiv - Biochemistry 2022Quote: ... A reaction was carried out of the enzyme and its corresponding trisaccharide dissolved in 1X GlycoBuffer 1 (New England Biolabs, n.d.), deionized water ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 μl of cells were used in a PCR reaction to obtain the gene for Sanger sequencing using Q5 (NEB, M0492). The PCR reaction was sent to Macrogen Europe B.V ...
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bioRxiv - Biochemistry 2022Quote: ... was cloned into pGex6P-1 in-frame with an N-terminal 3C-cleavable GST tag using HiFi assembly (New England Biolabs, USA).
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bioRxiv - Cancer Biology 2020Quote: ... 25uM RibOxi RT primer was then annealed to the reaction before ligation of 5’ RNA linker using T4 RNA ligase I at room temperature for 1 hour and subsequent reverse-transcription using Protoscript II (NE Biolabs, M0368S). cDNA was then purified with AmpureXP beads with 1:1.8 ratio ...
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bioRxiv - Microbiology 2020Quote: We tested four high-fidelity and/or long-range DNA polymerases in duplicate to evaluate improvements of read length during the amplification step: 1) NEBNext (NEB M0541), 2 ...
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bioRxiv - Immunology 2022Quote: ... then the single-stranded oligos (1 ng) were PCR amplified for 10 cycles with Q5 High-Fidelity DNA Polymerase (NEB, M0491L) using Oligo_Amp_F and Oligo_Amp_R primers to generate double-stranded DNAs ...
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bioRxiv - Genetics 2022Quote: ... run at 50 V for 18 hours at 4°C in Tris-acetate buffer at pH 8.0 with comparison to Quick-Load 1 kb Extend DNA ladder (NEB, Cat# N3239S). Unless otherwise stated ...
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bioRxiv - Microbiology 2019Quote: ... We added 1 mL of RAD reagent from the RAD002 kit and 0.2mL of Blunt TA ligase (New England Biolabs, MA, USA) and the reaction was incubated for 5 minutes at room temperature ...
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bioRxiv - Microbiology 2019Quote: Bacterial 16S rRNA genes were amplified from 50 ng DNA per sample diluted to 10 µL nuclease free water in 50 µL with 1 × LongAmp Taq master mix (New England Biolabs, Inc), 1 µL 16S rRNA gene barcoded primer (ONT-SQK-RAB-201 ...
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bioRxiv - Biochemistry 2019Quote: ... Immunoblotting was performed using HRP-conjugated anti-maltose binding protein (MBP) monoclonal antibody at 1:10000 dilution (New England Biolabs, #E8038) to verify equal expression levels of each construct.
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bioRxiv - Molecular Biology 2019Quote: ... Master mix was added to each sample together with 1 µl truncated T4 RNA Ligase 2 (#M0239, NEB; Frankfurt/Main, Germany). Ligation was carried out for 2 h at 23°C and nucleic acids were precipitated ...
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bioRxiv - Developmental Biology 2019Quote: ... of unc-3 and lin-39 were amplified and then ligated to cholinergic (cho-1, unc-3) or GABAergic (unc-47) promoters using Gibson Assembly Cloning Kit (NEB #5510S). For unc-3 RNAi ...