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Citations for New England Biolabs :
251 - 300 of 520 citations for Small Volume Spectrophotometer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... an adenylated 5’ end and a dideoxycytosine blocked 3’end – was ligated to size-selected small RNAs using T4 Rnl2tr K227Q (NEB, M0351L) for 16 hours at 25°C ...
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bioRxiv - Genomics 2021Quote: This material was used for generating sequencing libraries using the Small RNA library prep Set for Illumina (Multiplex compatible) kit (NEB E7330). Typically ...
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bioRxiv - Synthetic Biology 2021Quote: SynHox assemblon BACs were verified by digesting a ∼250-500ng purified by alkaline lysis (72) from small scale (5-10 mL) saturated bacterial culture with PvuI-HF (New England Biolabs R3150S). Digestion reactions were carried out at 37°C for 3-24 hours ...
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bioRxiv - Microbiology 2020Quote: RNA recovered from the PA-ac4C-seq and PAR-CLIP procedures were used for cDNA library preparation using the NEBNext Small RNA Library Prep Set for Illumina (NEB E7330S), then sequenced using Illumina NextSeq 500 ...
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bioRxiv - Molecular Biology 2020Quote: ... 100ng samples were submitted for library preparation using NEBNext® Small RNA Library Prep Set for Illumina (New England Biosciences (NEB), Ipswich ...
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bioRxiv - Neuroscience 2022Quote: ... The non-15N 4R tau used for small-scale chemical in vitro crosslinking was phosphorylated using cAMP-dependent Protein Kinase (PKA, catalytic subunit, New England Biolabs #P6000S) according to manufacturer guidelines (25uL reaction at 30°C for 2 hours with 200 μM ATP ...
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bioRxiv - Plant Biology 2021Quote: ... The sRNA libraries were constructed following manufacturer’s instruction using the NEBNext Small RNA Library Preparation set for Illumina (NEB, cat # E7300).
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bioRxiv - Genomics 2021Quote: MicroRNA-seq libraries were prepared using NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, cat. no. E7300) following manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... RNA aliquots were used for library preparation using NEBNext Multiplex Small RNA library preparation kit (New England Biolabs, Ipswich, MA, USA). The PCR amplified cDNA construct (from 140–160 bp ...
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bioRxiv - Microbiology 2022Quote: ... Purified RNAs were used for library preparation using the NEB Next Multiplex Small RNA Library Prep kit for Illumina (E7300 L) with Universal miRNA Cloning Linker from Biolabs (S1315S) as the 3’ adaptor and in-house designed indexed primers ...
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bioRxiv - Microbiology 2022Quote: ... 1 µg of RNA was used as input for library preparation using the NEBNext Multiplex Small RNA Library Prep Kit for Illumina (NEB E7560S), according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... Libraries were built from 250 ng of total RNA using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® following manufacturer’s recommendations (New England Biolabs). Thirteen PCR cycles were completed to finalize the library preparation ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were constructed from 800 ng total RNA (NEBNext Multiplex Small RNA library prep set for Illumina, New England Biolabs, NEB-E7560S) and the small RNA fraction was sequenced on the NextSeq 500 System (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... A range of cycle numbers was used to amplify the small RNA amplicons using Q5 High-Fidelity DNA polymerase (NEB M0491L). Amplified cDNA samples were loaded on 10% polyacrylamide gel (Bio-Rad 3450051) ...
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bioRxiv - Plant Biology 2023Quote: ... and small RNAs of 15-40 nt were excised from the gel and used to construct a library using “NEB Next Multiplex Small RNA Library Prep Set” for Illumina (New England Biolabs, E7300). High-throughput sequencing using the Illumina NextSeq500 platform was performed at BerryGenomics ...
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bioRxiv - Molecular Biology 2023Quote: ... cloning and data analysis were performed as previously described [38] except that the RPF libraries were prepared using NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB E7300). The trimmed RPF reads were mapped to C ...
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bioRxiv - Genomics 2023Quote: ... Included in the gel as controls were an RT reaction conducted in the absence of tRNA and a Small Range RNA Ladder (NEB N0364S). The gel was stained with SYBR gold and visualized using a ChemiDoc imager (BioRad) ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were constructed from 300 ng (liver) or 200 ng (hypothalamus) total RNA (NEBNext Multiplex Small RNA library prep set for Illumina, New England Biolabs, NEB-E7560S) and the small RNA fraction was sequenced on the NextSeq 500 System (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... the same amount of input RNA was loaded for library preparation using the NEBNext Multiplex Small RNA library preparation kit (New England Biolabs, USA). Libraries were size-selected for fragments 15-50 bp by gel electrophoresis ...
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bioRxiv - Cell Biology 2023Quote: ... One hundred nanograms of total RNA was used to prepare sequencing libraries using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB #E7560S). The 3’ adapter was ligated and the reverse transcription primer hybridized ...
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries for ribosome profiling were prepared following the NEBNext Multiplex Small RNA Library Prep kit for Illumina’s protocol (NEB, Cat# E7300S). Ribo-seq ...
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bioRxiv - Cancer Biology 2020Quote: ... a desired volume of genomic DNA was equally mock or digested with BamHI (New England BioLabs) for 4 h at 37°C ...
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bioRxiv - Genetics 2019Quote: ... the 25 μL reaction volume contained 1.0 unit of Taq DNA Polymerase (MO267S, New England BioLabs), 1 X ThermoPol Buffer ...
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bioRxiv - Cancer Biology 2019Quote: ... Beads were first treated with T4 PNK (NEB, M0201L; 20 U in 80 μl reaction volume) in the absence of ATP (37°C ...
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bioRxiv - Genomics 2021Quote: ... The remaining RNA was mixed with an equal volume of hydrophilic streptavidin magnetic beads (NEB S1421) which had been pre-washed two times and resuspended in 2M NaCl ...
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bioRxiv - Genomics 2021Quote: ... The DNA library was purified with 1X volume of NEBNext® Sample Purification Beads (NEB #E7103) and eluted with 40 μL of 1 mM Tris buffer (pH 7.5).
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of crude mitochondria were mixed with 1/10 volume gel loading dye (7025, NEB) and subjected to Semi-Denaturing Detergent Agarose Gel Electrophoresis (SDD-AGE) ...
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bioRxiv - Developmental Biology 2021Quote: ... which was then digested with HpyCH4IV (NEB; 2.5 units added to total reaction volume with NEB buffer 3.1 to 0.5x total ...
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bioRxiv - Cell Biology 2021Quote: ... Beads were first treated with T4 PNK (NEB, M0201L; 20 U in 80 μl reaction volume) in the absence of ATP (37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... The total volume of the A-tailing reaction was mixed with Quick Ligase buffer 2X (NEB), 3000 U of Quick Ligase (NEB ...
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bioRxiv - Molecular Biology 2023Quote: Endpoint PCR was performed in a 20 µL reaction volume containing 1× OneTaq Master Mix (NEB), 0.2 µM of each primer and 1 µL of 100-fold diluted cDNA ...
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bioRxiv - Microbiology 2023Quote: ... RNA samples were thawed and mixed with an equal volume of 2x RNA Loading Dye (NEB) and loaded onto the gels ...
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bioRxiv - Molecular Biology 2023Quote: ... to a final volume of 800 µl after washing it with 1x T4 ligase buffer (NEB) (2,500 xg ...
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bioRxiv - Microbiology 2024Quote: ... A 10 μL volume of 10-fold DNAse I reaction buffer (NEW ENGLAND BioLabs, catalogue-B0303S), 5 units (2.5 μL ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were mixed with 1/5th volume of 6X gel loading buffer (New England Biolabs®) and then electrophoresed in pairs alongside their undigested counterparts on an agarose gel as described below ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting small RNAs were used for library preparation with the NEBnext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, Cat# E7300) following the user’s manual ...
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bioRxiv - Systems Biology 2022Quote: The small RNA sequencing libraries were constructed using 3 μg total RNA per sample and NEB Next® Multiplex Small RNA Library Prep Set for Illumina® (NEB) following the manufacturer’s recommendation ...
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bioRxiv - Biochemistry 2021Quote: ... The relinearized single-stranded DNA template was subjected to PCR amplification by using barcoded primers for Illumina TrueSeq small RNA sample and Phusion High-Fidelity DNA Polymerase (2 U; M0530L, NEB, Ipswich, MA). Subsequently ...
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bioRxiv - Cancer Biology 2022Quote: The EV-RNA was compiled into cDNA libraries using a NEBNext Small RNA Library Prep Set for Illumina (Multiplex Compatible) (Cat No.: E7330, NEB, Ipswich, USA) following the manufacturer’ s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Small RNA libraries were prepared from the extracted 1ug total RNA or 100ng PIWIL1-antibody-pulled down RNA using NEBNext Multiplex Small RNA Library Prep Set for Illumina (Set 1) (NEB, Cat# E7300S) according to the manufacture’s protocol ...
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bioRxiv - Genetics 2021Quote: Sequencing libraries were prepared from either 500 ng total RNA or 3 µl TraPR-enriched [80] sRNA with the NEBNext Multiplex Small RNA Library Prep Set for Illumina (E7300; NEB, Ipswich, Massachusetts) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Preparation of a small RNA-Seq library from submitted total RNA sample was performed using the NEBNext® small RNA library preparation kit (New England Biolabs, UK). Small-RNA sequencing was conducted using the Illumina HiSeq 4000 (Illumina ...
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NSC-derived exosomes enhance therapeutic effects of NSC transplantation on cerebral ischemia in micebioRxiv - Neuroscience 2022Quote: Sequencing libraries of miRNAs of NSC-derived exosomes were produced using NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB, United States) following the previous report (Cai et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were constructed from 800 ng total RNA (NEBNext Multiplex Small RNA library prep set for Illumina, New England Biolabs, NEB-E7560S) and the small RNA fraction was sequenced on the NextSeq 500 System (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... libraries were constructed from 1000 ng total RNA (NEBNext Multiplex Small RNA library prep set for Illumina, NEB-E7560S, New England Biolabs) and the small RNA fraction sequenced (NextSeq 500/550 High Output Kit v2.5 75 Cycles ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were constructed from 300 ng (liver) or 200 ng (hypothalamus) total RNA (NEBNext Multiplex Small RNA library prep set for Illumina, New England Biolabs, NEB-E7560S) and the small RNA fraction was sequenced on the NextSeq 500 System (Illumina ...
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bioRxiv - Plant Biology 2024Quote: ... Sequencing libraries were generated using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina (E7330S, New England BioLabs, Ltd., USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Developmental Biology 2023Quote: ... the 3′ ends of small RNA were ligated to an adapter using T4 truncated RNA ligase (Lucigen, Cat# LR2D11310K and NEB, Cat# M0242L), followed by the ligation of the 5′-end adaptors by T4 ligase ...
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bioRxiv - Molecular Biology 2023Quote: ... Two hundred nanograms of RNA was used to generate sequencing libraries using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® Kit (New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... Small RNA sequencing libraries were prepared using the NEBNext Multiplex Small RNA Library Prep Set for Illumina following the manufacturer’s recommendations with the 3’ ligation step changed to 16°C for 18 hours to improve capture of methylated small RNAs (New England Biolabs, cat# E7300S). Small RNA PCR amplicons were size selected on 10% polyacrylamide non-denaturing gels ...