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Citations for New England Biolabs :
151 - 200 of 520 citations for Small Volume Spectrophotometer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... N is for a random nucleotide) were ligated to the small RNA by T4 RNA ligase 2 (NEB) and T4 ligase 1 (NEB ...
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bioRxiv - Genetics 2020Quote: ... TrueSeq-barcoded RNA-seq libraries were generated with the NEBNext Small RNA Library Prep Kit (New England Biolabs) and size selected for insert sizes ∼18-50bp ...
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bioRxiv - Microbiology 2021Quote: ... A small fraction of the refolded MBP-ACE2NTD fusion protein was further purified by amylose magnetic beads (NEB) according to the protocol provided by the manufacturer.
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bioRxiv - Molecular Biology 2021Quote: sRNA-seq libraries were prepared using the NEB Next Small RNA-sequencing kit (NEB #E7300, New England BioLabs), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: sRNA-seq libraries were prepared using the NEB Next Small RNA-sequencing kit (NEB #E7300, New England BioLabs), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... and used for library construction using NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: The sRNA-seq libraries were constructed with NEB Next Multiplex Small RNA Library Prep Set for Illumina (NEB). After agarose gel electrophoresis ...
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bioRxiv - Microbiology 2023Quote: ... Libraries were prepared using the NEBNext® Small RNA Library Prep Set for Illumina® (New England BioLabs), with the Universal miRNA Cloning Linker (New England BioLabs ...
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bioRxiv - Genomics 2023Quote: ... with the ligation of 3′-small RNA Tru-Seq adapter using the truncated T4 RNA Ligase 2 (NEB). 45-150 nt capped small RNAs were recovered on 15% Urea-TBE gel (Novex ...
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bioRxiv - Plant Biology 2019Quote: Library preparation was performed with the NEBNext® Multiplex Small RNA Library Prep Set for Illumina (New England BioLabs) according to the manufacturer’s instructions with few deviations ...
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bioRxiv - Evolutionary Biology 2020Quote: ... library preparation was carried out using NEBNext Multiplex Small RNA Library Prep Set for Illumina kit (New England Biolabs) with modified protocol23 for improving low-input samples and sequenced with NextSeq500 (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... Small RNA libraries were prepared according to the manufacturer’s protocol of the NEBNext Multiplex Small RNA Sample Prep Set for Illumina-Library Preparation (New England Biolabs). NEBNext Multiplex Oligos for Illumina Index Primers were used for library preparation (New England Biolabs) ...
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bioRxiv - Molecular Biology 2021Quote: ... Small RNA libraries were produced with NEBNext Multiplex SmallRNA Library Prep Kit for Illumina (E7560S, E7580, New England Biolabs) with the customisation of a dilution of primers 1:2 ...
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bioRxiv - Developmental Biology 2019Quote: ... This was used as input for the NEBNext Multiplex Small RNA Library Prep Set for Illumina kit (NEB #E7300) according to the manufacturer’s recommendations with the following modifications ...
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bioRxiv - Neuroscience 2021Quote: Twelve small RNA libraries representing 2 biological and 3 technical replicates from control- or BDNF-treated cortical neurons and corresponding EVs were prepared using NEBNext_Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs) as per manufacturer’s instructions and sequenced in Illumina HiSeq2000 ...
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bioRxiv - Molecular Biology 2021Quote: ... Purified small RNA samples were then digested using Nucleoside Digestion Mix (catalog no. M0649S, New England BioLabs, Beverly, MA). LC-MS/MS measurement of various post-transcriptional modifications were performed on a Waters XEVO TQ-STM (Waters ...
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bioRxiv - Molecular Biology 2021Quote: ... deep sequencing libraries were generated using the NEBNext Small RNA Library Prep Set for Illumina (E7560, New England Biolabs) and sequenced on an Illumina Hiseq4000 ...
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bioRxiv - Cell Biology 2022Quote: ... Complementary DNA libraries were then prepared using a range of mitochondrial RNA inputs (final input range 1.8-120 ng) using the NEBNext Multiplex Small RNA Library Prep Set (New England Biolabs.). To investigate the efficiency of small RNA library preparation across a range of mitochondrial RNA inputs ...
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bioRxiv - Genomics 2019Quote: ... The reverse transcribed samples were subjected to PCR for 15 cycles by adding 50μL of the PCR mastermix from the NEB small RNA for Illumina library prep kit (NEB), 5μL of the Universal adapter and 5μL of a unique indexed adapter (see NEB library pooling guidelines for suitable adapter combinations for three sample sequencing runs) ...
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bioRxiv - Molecular Biology 2019Quote: ... Total RNA of RIN (RNA integrity number) more than 7 is subject to NEBNext Small RNA library kit (NEB). After final PCR amplification with indexed-adaptors ...
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bioRxiv - Molecular Biology 2020Quote: ... The sequencing library was generated by NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA), with added index codes to attribute sequences of each sample as recommended by the manufacturer ...
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bioRxiv - Developmental Biology 2020Quote: ... Sequencing libraries were generated using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA fragments were converted to library using NEBNext® Small RNA Library kit (ref#E7330S, New England Biolabs, UK) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Small RNA libraries were prepared according to the manufacturer’s protocol of the NEBNext Multiplex Small RNA Sample Prep Set for Illumina-Library Preparation (New England Biolabs). The required number of PCR cycles to amplify library is determined by running PCR for 10-15 cycles and analyzing PCR products on a 6% PAGE gel ...
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bioRxiv - Microbiology 2022Quote: ... Point mutations or small deletions in pGM34 and pGM71 (Table 1) were performed using the KLD enzyme mix (NEB)
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bioRxiv - Genomics 2023Quote: ... libraries were constructed with the NEBNext Small RNA Library Preparation Kit according to manufacturer’s recommendations (NEB, cat. no. E7330). See Supplemental File 6 for detailed protocol.
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bioRxiv - Genetics 2023Quote: ... Small RNA libraries were prepared according to the manufacturer’s protocol of the NEBNext Multiplex Small RNA Sample Prep Set for Illumina-Library Preparation (New England Biolabs). NEBNext Multiplex Oligos for Illumina Index Primers were used for library preparation (New England Biolabs) ...
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bioRxiv - Genetics 2023Quote: Libraries were prepared according to the manufacturer’s protocol of the NEBNext Multiplex Small RNA Sample Prep Set for Illumina-Library Preparation (New England Biolabs). NEBNext Multiplex Oligos for Illumina Index Primers were used for library preparation (New England Biolabs) ...
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bioRxiv - Molecular Biology 2023Quote: ... sequencing libraries were generated using a NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... The remaining volume was submitted for biotin-enrichment on magnetic Streptavidin beads (NEB S1420S), by vortexing for 90 min at RT ...
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bioRxiv - Synthetic Biology 2020Quote: ... in a final volume of 20 µL (both enzymes were from New England Biolabs). The mixture was then incubated at 37 °C for 1 h ...
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bioRxiv - Evolutionary Biology 2022Quote: ... equal volumes of pre-annealed gRNA and SpCas9 (20 µM, NEB Cat. No. M0646M) are mixed and incubated for 1 h at RT to form the RNP ...
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bioRxiv - Biophysics 2023Quote: ... Lysates were applied to 10-mL bed volume of chitin resin (New England BioLabs) that was pre-equilibrated with column buffer for 1.5 hours at 4°C on a tube rotator ...
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bioRxiv - Cancer Biology 2023Quote: ... A volume of 40 μl reaction mix containing 5 μl isothermal amplification buffer (NEB), 3 μl 100 mM MgSO4 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were harvested and resuspended with 1:10 volumes of CutSmart Buffer (NEB, # B7204) and 5 µl of 100 mg/ml RNase-A solution (pre-heated at 100°C for 20 minutes) ...
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bioRxiv - Developmental Biology 2024Quote: All PCR reactions were done in 50𝜇l volume using Q5 high fidelity polymerase (NEB) following NEB Q5 high fidelity PCR protocol ...
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bioRxiv - Genomics 2024Quote: ... and mixed with an equal volume of 2 x RNA loading dye (NEB, B0363S). 25 μl of the mixture was loaded on a 10% TBE-Urea gel (Bio-Rad ...
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bioRxiv - Immunology 2024Quote: ... 1 µl per 50 µl pool volume of Thermolabile Exonuclease I (New England Biolabs) was then added and incubated for 4 minutes at 37 °C ...
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bioRxiv - Genetics 2021Quote: ... 1 μg of total Trizol-purified RNA purified was used for library preparation using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB) with libraries indexed in 12 cycles PCR ...
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bioRxiv - Genomics 2020Quote: ... Libraries were prepared from 1000 ng total RNA using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs) by the Transcriptional Regulation and Expression Facility at Cornell University.
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bioRxiv - Genomics 2020Quote: Small RNA sequencing libraries were generated using the NEBNext Small RNA library Prep kit and NEBNext multiplex oligos for Illumina according to the manufacturer’s instructions (New England Biolabs, E7300). The final small RNA libraries were purified from 6% PAGE gel ...
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bioRxiv - Neuroscience 2020Quote: Six µL of each sample were used for library preparation with the NEBNext Multiplex Small RNA Sample Preparation Set for Illumina (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... Indexed sRNA libraries were constructed from these enriched sRNA fractions with the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... and 7 μl were used as input for NGS library preparation using the NEBNext Small RNA Library Prep Set for Illumina (New England Biolabs). Sequencing was performed in a MiSeq benchtop sequencer for 200 cycles ...
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Scanning mutagenesis of RNA-binding protein ProQ reveals a quality control role for the Lon proteasebioRxiv - Microbiology 2021Quote: Library preparation from co-immunoprecipitated RNA samples were carried out with NEBNext Multiplex Small RNA Library Prep Set (New England Biolabs) that allow sequencing in Illumina platforms ...
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bioRxiv - Plant Biology 2021Quote: ... miR158 OE and pseudo-PPR OE (one of these libraries failed the quality control).Libraries were prepared using NEB Next Small RNA Library Prep Set (New England Biolabs) and sequencing was carried out using Illumina HiSeq 4000 in single-end mode by outsourcing ...
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bioRxiv - Microbiology 2020Quote: ... Equal amounts of RNA (~50 ng) were used for preparation of cDNA libraries with the NEBNext Multiplex Small RNA Library Prep kit for Illumina (NEB) with some minor changes to the manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2020Quote: Many of the reagents used in 5XP-seq are included in the NEBNext Multiplex Small RNA Library prep kit for Illumina (New England Biolabs), and are referred to as ‘NEB-kit’ below ...
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bioRxiv - Microbiology 2021Quote: ... Indexed sRNA libraries were constructed from these sRNA fractions with the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Small RNA deep sequencing libraries were generated using the NEBNext Small RNA Library Prep Set for Illumina (E7560, New England Biolabs), using 1 µg RNA as input ...