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Citations for New England Biolabs :
451 - 500 of 520 citations for Small Volume Spectrophotometer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Amplified DNA was then size enriched using 0.9X volume SPRI beads and subject to another round of PCR using 10x cycles with NEBNext Ultra II (NEB M0544S) using primers pairs cTF383+cTF399 for the promoter and oSA021+oSA023 for the gene body to add Illumina Read1 and Read2 overhangs ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.25 µM of forward and reverse primers mixed in a final volume of 25 µl reaction using Q5 Hot Start High-Fidelity 2X Master Mix (NEB), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... the reaction was stopped with a final concentration of 50 mM EDTA and the proteins were removed by treatment with proteinase K (1/100 of the volume – NEB) and SDS (0.1% ...
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bioRxiv - Microbiology 2024Quote: ... reactions were performed in a final volume of 100 μl using the PURExpress ΔRibosome In vitro Protein Synthesis Kit (New England Biolabs) according to the manufacturer instructions and with ribosomes purified from E ...
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bioRxiv - Immunology 2024Quote: ... 10 ng of restriction enzyme digested backbone and 20 ng of SPRI purified PCR variable regions were combined with an appropriate volume of 2x HiFi master mix (New England Biolabs) for one hour at 50°C ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions were quenched with the addition of an equal volume of 2X RNA loading dye buffer and 0.8 units of proteinase K from Tritirachium album (New England Biolabs Inc.) were added ...
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bioRxiv - Molecular Biology 2024Quote: ... Each methylation reaction was thoroughly mixed on ice using 100uL of cell methylation buffer (10% volume 10x M.CviPI reaction buffer (NEB, B0227S), 300 mM sucrose ...
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bioRxiv - Molecular Biology 2024Quote: ... adding nuclease-free water to a final volume of 1 L) with 1:1000 (vol/vol) proteinase K (New England Biolabs) at 37°C for 1 h ...
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bioRxiv - Genomics 2022Quote: ... Strand-specific RNA sequencing library and small RNA sequencing libraries were generated respectively using NEBNext® Ultra™ RNA Library Prep Kit and NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, Ipswich, MA, USA). After the cDNA synthesis and PCR amplification ...
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bioRxiv - Microbiology 2020Quote: ... 1 µg of plasmid was mixed with 10 U of the corresponding restriction enzymes and the corresponding amount of 1X restriction buffer in final 50 µL volume (New England Biolabs, USA). Reactions were incubated at least for one hour at the recommended temperature ...
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bioRxiv - Cell Biology 2020Quote: ... 250 ng of plasmid DNA was used per PCR reaction and used in a volume of 50 µl using Next High-Fidelity 2x PCR Master Mix (NEB, M0541) (according to the manufacturer’s protocol) ...
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bioRxiv - Genetics 2021Quote: ... 1ul of cDNA was used to perform a first round of PCR (20ul volume) with Taq DNA polymerase (New England BioLabs, M0273) for 20-25 cycles and primers listed in Table 2 ...
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bioRxiv - Neuroscience 2021Quote: ... Quantitative real-time PCR was performed with 20 ng of cDNA in a total reaction volume of 10 μL using Luna® Universal qPCR Master Mix (Cat. No. M3003L; New England BioLabs) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... The ITS2 region was amplified and Illumina adapters appended by PCR in 25 ul volume with Q5 High-Fidelity 2X master mix (NEB, # M0492L). PCR conditions were as follows ...
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bioRxiv - Genomics 2021Quote: Capping with 3’ Desthiobiotin GTP (DTB-GTP) was performed in 50 µl total volume with 5 µL Vaccinia capping enzyme (NEB M02080) and 0.5 mM DTB-GTP (NEB N0761) ...
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bioRxiv - Biochemistry 2020Quote: ... Beads were resuspended in 1 volume of buffer A / 300 mM NaCl / 2 mM MnCl2 / 1 mM DTT and incubated with λ protein phosphatase (NEB) at 50 U / mL for 1 hour at 23°C with agitation ...
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bioRxiv - Microbiology 2021Quote: ... The amplification of bacterial DNA was achieved by targeting the V3–V4 region of 16S rRNA gene with 30 µL final volume containing 15 µL of 2× master mix (BioLabs, USA), 3 µL of template DNA ...
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bioRxiv - Microbiology 2021Quote: All PCR assays were carried out in 25 μL total reaction volumes using 2x Mastermix (New England Biolabs Inc., Ipswich, USA), 1.25 μL oligonucleotide primes (10 μM ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 µg of GST-beads were incubated for 15 min on ice in GRB buffer in a 25 μl reaction volume with 1 µg of 100 bp DNA ladder (New England Biolabs # N3231S) or mononucleosome (Active motif # 81070) ...
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bioRxiv - Molecular Biology 2019Quote: For the oligo-dT affinity chromatography 0.5 ml of C1 or P2 fractions were incubated with an equal volume of a 50% slurry of oligo(dT) beads (NEB #S1408S) in Phy buffer ...
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bioRxiv - Microbiology 2020Quote: ... Colony PCRs were carried out in 12.0 μL final volumes containing: 5 μL of high-fidelity OneTaq® QuickLoad® 2X Master Mix (New England Biolabs), appropriate forward and reverse control primers (both 0.4 μM ...
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bioRxiv - Genetics 2020Quote: ... Each reaction was run in 10μL volumes and included 1x Luna Universal qPCR Master Mix (New England BioLabs, Ipswich, Mass. USA), optimal forward and reverse concentrations were experimentally determined (200nM for both forward and reverse primers for 17S ...
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bioRxiv - Genetics 2020Quote: ... PCR amplifications were done in 5 μL volumes in a 384-format PCR plate using Q5® Hot-Start High-Fidelity 2x Master Mix (New England BioLabs, 2.5 μL per reaction for 1x concentration ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and both PCRs were carried out in 5μl total volume using the NEBNext Ultra II Q5 Mastermix (New England Biolabs, Ipswich, MA). The final amplicons were again purified ...
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bioRxiv - Microbiology 2021Quote: ... tPCR reactions for each target were performed in a 50 μl volume consisting of 25 μl LongAmp® Taq 2X Master Mix (NEB), 200 nM each of tPCR primers and 100 ng DNA template as follows ...
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bioRxiv - Immunology 2022Quote: ... 100 µl volume) using primers described in Table S3C and Phusion® High-Fidelity DNA Polymerase (New England Biolabs, Beverly, MA). This provides approximately 300X coverage for sequencing ...
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bioRxiv - Microbiology 2023Quote: ... The sonicated DNA was end-repaired for 30 minutes at 20°C in 200 μl final volume containing 1x end-repair buffer and 10 μl of end-repair mix (NEB, E6050L). The reaction was cleaned in 2x AmpureXP Beads (Beckman Coulter ...
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bioRxiv - Genetics 2023Quote: ... 2.5 µl of homogenate (1/100th homogenate volume) was removed and kept on ice in a total of 300 µl 1X Monarch DNA/RNA Protection Reagent (NEB, T2011L) as the input sample ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCRs were conducted in volumes of 30 μL comprising a Phusion® High-Fidelity PCR Master Mix (New England Biolabs) (15 μL) ...
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bioRxiv - Microbiology 2022Quote: ... The PCR reaction volume used was 50 μl: 0.2 μl of Q5® high-fidelity DNA polymerase (New England Biolabs, UK), 10 μl of Q5® reaction buffer (5X ...
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bioRxiv - Microbiology 2023Quote: ... Two μl of a 1:250 dilution of the annealed oligos were ligated to 50 ng of dephosphorylated BsaI-digested vector at 16°C overnight in 20 μl final volume containing 1x T4 DNA ligation buffer (NEB, B0202S), and 400U T4 DNA ligase (NEB ...
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bioRxiv - Genomics 2023Quote: ... The gDNA is eluted with 25.2uL ddH2O and undergoes a second round of in vitro CpG methylation with previously described parameters above with exception that 10x Mg2+-free buffer is replaced with equal volume of NEB Buffer #2 (New England Biolabs, B7002S). The reaction is incubated again for 4 hours at 37°C ...
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bioRxiv - Genomics 2024Quote: ... IVT reactions were set up in 30 μl total volume with the HiScribe T7 High Yield RNA Synthesis Kit (NEB E2040S) with 2 μl of each NTP ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR reactions were performed in a 50 μl volume containing Q5® Hot Start High-Fidelity DNA Polymerase (New England Biolabs). Quantitative analysis of the purified libraries was performed using Qubit 3.0 Fluorometer and Qubit® dsDNA HS Assay kit (InvitrogenTM) ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR reaction was carried out in the volume of 50 µL including 25 µL 2X OneTaq Master Mix (NEB, #M0482), 2 µL of 5 µM i5 and i7 each ...
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bioRxiv - Biochemistry 2024Quote: ... the crude extract containing 100 μg (in 100 μL volume) of proteins was treated with 400 units of λ-PPase (#P0753; New England Biolabs) for 60 min at 30 °C ...
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bioRxiv - Genomics 2022Quote: ... to 5 μg of input DNA (in total volume of 24 μl at >210 ng/μl) with 3 μl 10X CutSmart Buffer (NEB, Cat #B7204), and incubating for 10 min at 37°C ...
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bioRxiv - Genomics 2020Quote: ... Assembly was performed with 1650 ng of BsmBI-digested pLentiCRISPRv2 and 250 ng of amplicon in a total reaction volume of 100 μL with HiFI DNA Assembly Mix (NEB, Ipswich MA) for 30 min at 50°C ...
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bioRxiv - Molecular Biology 2019Quote: ... and then enriched via PCR amplification in a total volume of 50 μL containing 3 μL of NEB Next USER Enzyme (NEB, Ipswich, USA), 25 μL of NEB Next High-Fidelity PCR Master Mix (2× ...
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bioRxiv - Genomics 2021Quote: ... 1 µg of genomic DNA was digested in a 40 µl volume of 1x CutSmart buffer with 1 µl of MFRE (MspJI or FspEI; New England Biolabs, Ipswich, MA) and 1.4 µl activator oligonucleotide (15 µM stock ...
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bioRxiv - Microbiology 2020Quote: ... PCRs were performed in a final volume of 20 μl including NEB Luna Universal Probe One-Step Reaction Mix and Enzyme Mix (both New England Biolabs, Herts, UK) and 5 μl of RNA sample ...
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bioRxiv - Microbiology 2021Quote: ... PCRs were performed in 25 μL reaction volumes and contained 13 μL Q5® Hot Start High-Fidelity 2× Master Mix (New England Biolabs, US), 0.5 μM of each primer and 0.4 μL of 25 mg/mL BSA ...
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bioRxiv - Microbiology 2021Quote: ... PCR with Taq polymerase was performed in a 25-μl volume tube following the manufacture’s guidelines (New England Biolabs, Ipswich, MA, www.neb.com). PCR amplicons were visualized by agarose gel electrophoresis ...
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bioRxiv - Microbiology 2020Quote: ... PCR was performed in a final volume of 50 μL: 25 μL of Q5 polymerase master mix (New England Biolabs, MA, USA), 10 μL of GC enhancer buffer (New England Biolabs) ...
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bioRxiv - Microbiology 2020Quote: ... Five hundred nanograms total RNA was then reverse-transcribed in 20 μl reaction volume using the LunaScript RT SuperMix Kit (E3010, New England Biolabs, MA, USA). Quantitative PCR was performed by transferring 2 μl of the RT mix to the qPCR mix prepared with Luna Universal qPCR Master Mix (M3003 ...
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bioRxiv - Zoology 2019Quote: ... PCR reactions were performed in a total volume of 50 µL containing 10 µL NEB 5X Q5® Buffer (New England BioLabs, USA), 10 µL NEB 5X Q5® High GC Enhancer (New England BioLabs ...
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bioRxiv - Biophysics 2022Quote: RE digestion reactions are prepared by adding 1/10th of the final reaction volume of 10x reaction buffer supplied by the RE manufacturer (typically NEB CutSmart buffer) and 0.1% Tween-20 to the DNA solution (diluted to the desired concentration in nanopure DI water) ...
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bioRxiv - Microbiology 2022Quote: ... immitis and Wolbachia qPCR assays were carried out in 10 μL final volumes of Luna® Universal Probe qPCR Master Mix (New England Biolabs, Australia) containing 1 μL of template DNA isolated from whole dog blood and primers and probe at a final concentration of 400 nM and 100 nM ...
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bioRxiv - Immunology 2023Quote: ... Each PCR (performed in triplicate for each sample) was done in a 25 μl volume using Q5 High-Fidelity 2X Master Mix (NEB, cat# M0492L), 5 pmol of each primer and 50 ng template DNA ...
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bioRxiv - Microbiology 2023Quote: ... and lysed with 2× concentrated lysis buffer (0.25% Triton X-100, 50 mM KCL, 100 mM Tris-HCl pH 7.4, 40% glycerol, 1/50 volume of RNase inhibitor; NEB, Cat. No. M0314S) at room temperature for 10 min ...