Labshake search
Citations for New England Biolabs :
251 - 300 of 4933 citations for Copper;5 10 15 20 tetrakis 4 methylphenyl porphyrin 22 24 diide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2024Quote: ... pET22(b) vector harboring NKp46 construct 22-212 was used to transform chemically competent T7 Express cells (NEB, US) following protocols provided by the manufacturer ...
-
bioRxiv - Genomics 2022Quote: ... and 15 units of Quick CIP (NEB) by incubating at 37°C for 20 min followed by Quick CIP inactivation at 80°C for 3 min ...
-
bioRxiv - Biochemistry 2020Quote: ... 15 units of Exonuclease V (RecBCD; NEB) and T5 exonuclease (NEB ...
-
bioRxiv - Synthetic Biology 2021Quote: ... 15 units of T4 DNA polymerase (NEB) and 5 units of Klenow DNA polymerase (NEB ...
-
bioRxiv - Molecular Biology 2022Quote: ... followed by a 15 min DNaseI (NEB) digestion at 37°C ...
-
bioRxiv - Genetics 2022Quote: ... followed by 15 min DNAse I (NEB) treatment at 37°C ...
-
bioRxiv - Biophysics 2021Quote: ... 15 μL T4 ligase (NEB, cat#M0202S), 30 μL T4 ligase buffer and ...
-
bioRxiv - Cell Biology 2024Quote: ... amplified by PCR (15 cycles, Phusion, NEB) and cloned into the XhoI and EcoRI sites of pSC2 as previously described(38 ...
-
bioRxiv - Microbiology 2020Quote: ... 20 μl proteinase K (20 mg/ml) (New England Biolabs) and 25 μl 10sSodium dodecyl sulphate (SDS ...
-
bioRxiv - Molecular Biology 2021Quote: ... for 12 h at 4 °C with 10 μg of Factor Xa protease (New England Biolabs, Hitchin, UK) per 1 g of mitochondria ...
-
bioRxiv - Biophysics 2024Quote: ... A 4°C AKTA Pure FPLC system (Cytiva) was prepared with a 10 mL amylose column (NEB #E8021S) and 2 mL/min flow rate ...
-
bioRxiv - Microbiology 2020Quote: ... 1 μL of 5 μM annealed adaptors and 10 μL of Blunt/TA ligase master mix (NEB) was added to each reaction ...
-
bioRxiv - Developmental Biology 2020Quote: ... Free adaptor was then removed by addition of 1 μL 10 U/μL 5’-Deadenylase (NEB, M0331S). 1 μL 10 U/μL RecJ Exonuclease (Lucigen/Epicentre ...
-
bioRxiv - Microbiology 2024Quote: ... then combined with 5 μM dNTPs and 2 μL of 10× rCutSmart buffer (NEB, Ipswich, MA, USA) in a total reaction volume of 17 μL ...
-
bioRxiv - Plant Biology 2024Quote: ... as described in the NEB Golden Gate Assembly protocol (5-10 inserts protocol) (NEB, MA, United States). E ...
-
bioRxiv - Systems Biology 2020Quote: ... we first phosphorylated the 5’ ends of each probe set by combining 4 μL of the pooled oligos with 1 μL T4 PNK (NEB), 20 μL T7 DNA ligase reaction buffer (NEB) ...
-
bioRxiv - Bioengineering 2022Quote: ... the two primers which contain the gRNA and have 4 nt overhang at 5’-end were annealed (3) the annealing product was ligated with PaqC I (New England Biolabs)-digested pMM002P or pMM005 ...
-
bioRxiv - Biochemistry 2023Quote: ... The duplex hRNase 4 cleavage products (5′ fragments of the RNA) were enriched using Hydrophilic Streptavidin Magnetic Beads (New England Biolabs). The beads were washed twice by a high salt buffer (5 mM Tris HCl ...
-
bioRxiv - Neuroscience 2024Quote: ... slides were rinsed three times with deionized water for 5 minutes and incubated in 1x Terminal Transferase (TdT) buffer containing 1X buffer 4 (NEB) and 2.5 μM CoCL2 for 10 minutes at room temperature in a humid chamber ...
-
bioRxiv - Neuroscience 2024Quote: ... Pellets were resuspended in NEB buffer prior to centrifugation at 11,500 rpm for 20 minutes at 4°C on a sucrose gradient (1.6M sucrose in NEB and 0.8M sucrose in NEB). Nuclei-containing pellets were fixed with 0.3% formaldehyde in NEB and rotated at room temperature for 10 minutes ...
-
bioRxiv - Microbiology 2024Quote: ... Decapping of the 5’ DTB-GTP cap was performed to leave the 5’ monophosphate for ligation by adding 20 μl of capped RNA to 2.2 μl of 10X ThermoPol buffer (NEB product number B9004) and 2 μl RppH (NEB product number M0356S) ...
-
bioRxiv - Cell Biology 2020Quote: ... Quantification of mRNA level was performed in a 20 μl mixture consisting of 10 μl Q5 High-Fidelity 2X Master Mix (NEB), 0.2 μl RT-PCR product ...
-
bioRxiv - Developmental Biology 2021Quote: ... Following the adaptor ligation each sample was mixed with 20 μl of a 2x DpnII Digestion Buffer and 10 units (1 μl) of DpnII restriction enzyme (New England Biolabs) mastermix and were incubated for 3 hours at 37 °C ...
-
bioRxiv - Microbiology 2021Quote: ... and either stored at −20°C or 10 μg used for immunoblots with an Anti-IkBa antibody (New England Biolabs) (54).
-
bioRxiv - Microbiology 2020Quote: ... The PCR was performed in a final volume of 20 μL: 10 μL of Q5 polymerase master mix (New England Biolabs), 0.5 μL of forward primer 10 uM ...
-
bioRxiv - Microbiology 2020Quote: ... each reaction tube of 20 μl contained 10 μl of Q5 High-Fidelity 2× Master Mix (New England BioLabs Inc.), 20 pmol of forward and reverse primer ...
-
bioRxiv - Genomics 2021Quote: ... The PCR mix (8 µl H2O, 2 µl primer mix P5Solexa/P3Solexa, 10 µM each, 20 µl Phusion HF Mix [New England Biolabs]) was added to 10 µl cDNA ...
-
bioRxiv - Microbiology 2020Quote: ... each reaction tube of 20 µl contained 10 µl of Q5 High-Fidelity 2× Master Mix (New England BioLabs Inc.), 20 pmol of forward and reverse primer ...
-
bioRxiv - Microbiology 2023Quote: ... Samples were boiled for 10 min and 2 μL of 20 mg/ml proteinase K (New England Biolabs, Cat. #P8107S) were added ...
-
bioRxiv - Cancer Biology 2023Quote: ... lysate volume equivalent to 20-40 µg total protein was first denatured at 100°C for 10 min in 1X glycoprotein denaturation buffer (NEB). Endo H digestion was performed at 37°C for 1h in the presence of 1X GlycoBuffer 3 and 1 µL of Endo H enzyme (NEB) ...
-
bioRxiv - Molecular Biology 2023Quote: Equilibration buffer (GF150 buffer supplemented with 0.1 mM Mg-ATP and 10% glycerol) was used to equilibrate 20 μL of SNAP-Capture Magnetic Beads (New England Biolabs) slurry in 1.5 mL Protein Lo Bind Tubes (Eppendorf) ...
-
bioRxiv - Microbiology 2023Quote: ... at a final volume of 20 μL with 10 μL of Luna Universal qPCR Master Mix (New England Biolabs, Germany), which uses SYBR Green chemistry ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1× CutSmart buffer (50 mM Potassium Acetate, 20 mM Tris-acetate, 10 mM Magnesium Acetate, 100 µg/ml BSA or recombinant albumin; NEB), 0.5 mM 1,4-Dithiothreitol (DTT) ...
-
bioRxiv - Molecular Biology 2023Quote: ... the remaining 5’-phosphate groups were dephosphorylated using calf-intestinal alkaline phosphatase for 10 minutes at 37°C and finally heat inactivated for 20 min at 80°C (Quick CIP, New England Biolabs). The Rap1 arrays initially cloned in a pUC19-derived vector (Table S2 ...
-
bioRxiv - Biophysics 2023Quote: ... The reaction was mix using 1× CutSmart buffer (50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate, and 100 μg/mL BSA; NEB) and 0.5 mM DTT (1,4-dithiothreitol ...
-
bioRxiv - Genetics 2023Quote: ... via a 20 µL PCR that utilized the following: 10 µL Onetaq Quick-Load 2x master mix (New England Biolabs), 10 µg bovine serum albumin ...
-
bioRxiv - Molecular Biology 2024Quote: ... Linearization was performed by overnight digest at 37C° of 10 µg of donor plasmid using 20 Units of PaqCI (R0745, NEB) in 1x rCutSmart buffer (B6004S ...
-
bioRxiv - Biochemistry 2024Quote: ... 2:1 molar ratio of trimerization domain:plasmid were ligated at 37°C for 20 minutes in a 10 µl mixture reaction containing: 1 μL T4 ligase buffer (NEB), 0.5 μL PaqCI (NEB) ...
-
bioRxiv - Biophysics 2021Quote: ... After 24 hours SNAP-Cell 647-SiR substrate (New England Biolabs) was added to the medium at a concentration of 3 μM ...
-
bioRxiv - Microbiology 2020Quote: ... The RNA fragments were ligated with 3’-barcoded adaptors at 22 °C for 1 h and 30 min using T4 RNA Ligase (NEB). Barcoded RNA samples were pooled together ...
-
bioRxiv - Immunology 2022Quote: ... Ligation of the fragmented RNA to the MARS-seq adapter was performed at 22°C for 2h with T4 RNA ligase (NEB) followed by a 1.5X AMPure XP bead cleanup ...
-
bioRxiv - Cancer Biology 2022Quote: ... and sgRNA cassettes were amplified using 22 cycles of PCR using NEBNext Ultra II Q5 PCR MasterMix (New England Biolabs). Sequencing was performed on a NovaSeq 6000 (Illumina ...
-
bioRxiv - Microbiology 2024Quote: Codon-optimized full-length SARS-CoV-2 S protein gene was cloned into Topo cloning vector and 22 N-glycosylation sites were mutated individually from N to Q using a Q5 Site-Directed Mutagenesis Kit (NEB). The full-length or C19 truncated fragments were amplified and inserted into plvx-puro vector ...
-
bioRxiv - Genomics 2023Quote: ... Ligated junctions were pulled-down with Dynabeads® MyOne™ Streptavidin C1 beads for 15 min at RT and DNA ends were A-tailed with 15 Units of Klenow exo- (cat. M0212L, NEB). Barcoded PerkinElmer adapters (cat ...
-
bioRxiv - Cell Biology 2023Quote: ... Ligated junctions were pulled-down with Dynabeads® MyOne™ Streptavidin C1 beads for 15 min at RT and DNA ends were A-tailed with 15 Units of Klenow exo- (cat. M0212L, NEB). Barcoded PerkinElmer adapters (cat ...
-
bioRxiv - Bioengineering 2020Quote: ... and 15 uL of H2O) (New England Biolabs) for 1 hour at 37°C followed by DNase I (10 µL DNase I ...
-
bioRxiv - Genomics 2024Quote: ... and 15 U Klenow exo (New England Biolabs), for 30 min at 37°C before inactivation for 20 min at 65°C ...
-
bioRxiv - Genomics 2024Quote: ... 15 U T4 polynucleotide kinase (New England Biolabs), 5 U Klenow DNA polymerase (Roche) ...
-
bioRxiv - Genomics 2023Quote: ... 15 μL 2x Q5U Master Mix (NEB M0597S), 0.4 μL 100 μM Nextera P5 index primer and 0.4 μL 100 μM Nextera P7 index primer ...
-
bioRxiv - Cell Biology 2024Quote: ... at 15 minutes before nuclear envelope breakdown (NEB) no NSC had begun the rounding up process that marks mitotic entry ...