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Citations for New England Biolabs :
2801 - 2850 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were designed in SnapGene and mutants were generated via Gibson assembly (Gibson Assembly® Cloning Kit from New England Biolabs)57,58 or via QuickChange (Q5® Site-Directed Mutagenesis Kit from New England Biolabs).59 Recombinant expression of motors in E ...
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bioRxiv - Genomics 2022Quote: ... The NEBNext® Ultra DNA Library Prep kit for Illumina (cat# NEB #E7370S/L) was used to process the DNA samples ...
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bioRxiv - Genomics 2022Quote: ... Extracted RNA was fragmented using the NEBNext Magnesium RNA Fragmentation module kit (NEB, USA), heating for 2min ...
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bioRxiv - Immunology 2022Quote: ... All fragments were assembled with NEBuilder HiFi DNA Assembly Cloning Kit (E5520S, NEB, USA), transformed into DH5-α Competent E ...
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bioRxiv - Developmental Biology 2022Quote: ... or from COV434 cells and hiPSCs using the Monarch Total RNA Miniprep kit (NEB). For experiments involving TF overexpression ...
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bioRxiv - Plant Biology 2022Quote: ... was constructed using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, MA, USA) by multi-DNA fragment assembly between (i ...
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bioRxiv - Plant Biology 2022Quote: ... and was done using an NEBNext Ultra RNA Library Prep Kit for Illumina (NEB). After cluster generation on a cBot cluster generation system using Truseq PE Cluster kit (Illumina) ...
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bioRxiv - Microbiology 2022Quote: ... Ribosomal RNA was depleted with NEBNext rRNA Depletion Kit for Bacteria (New England Biolabs). Barcoded libraries were made with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) ...
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bioRxiv - Molecular Biology 2022Quote: ... The plasmids were isolated by using a Monarch Plasmid Miniprep kit (New England Biolabs) according to the manufacturer’s protocol and PCR amplified using primers MMEJ R1 and MMEJ R2R ...
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bioRxiv - Microbiology 2022Quote: Plasmid DNA was prepared using a Monarch® Plasmid Miniprep Kit (NEB, cat# T1010). Genomic DNA was prepared using the DNeasy Blood & Tissue Kit (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was purified using the Monarch® PCR & DNA Cleanup Kit (NEB, cat# T1030). PCR was performed using either KAPA HIFI 2X ready mix (KAPA Biosystem ...
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bioRxiv - Microbiology 2022Quote: ... Bt1760 and Bt1761 variants were generated using the Q5 site directed mutagenesis kit (NEB). All constructs were verified by Sanger sequencing.
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bioRxiv - Molecular Biology 2022Quote: ... Library preparation was carried out using the NEBNext Small RNA Library kit (NEB, UK), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... RNA was fragmented using the NEBnext mRNA first and second strand synthesis kits (NEB). One to three biological replicates were generated for each condition and on average ~10 million reads were generated per sample.
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bioRxiv - Molecular Biology 2022Quote: ... with the NEBNext® Library Quant Kit for Illumina® (New England Biolabs, UK) and diluted to 1 nM ...
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bioRxiv - Molecular Biology 2022Quote: ... high yield in vitro transcription (HiScribe T7 Quick High Yield RNA Synthesis Kit, NEB), and cDNA synthesis reactions (Maxima H Minus Reverse Transcriptase ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... ΔDEP Venus-DVL2 was cloned with the Q5 Site-Directed Mutagenesis kit (NEB #E0554S) using Venus-DVL2 as a template ...
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bioRxiv - Neuroscience 2022Quote: Library was prepared using the NEBNext Ultra Library prep Kit (E7645S, New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2022Quote: ... Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (NEB Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (NEB Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the template was used for in vitro transcription using EnGen sgRNA synthesis Kit (NEB), and the reaction was cleaned up using the MEGACLEAR Transcription Clean-Up KIT (Thermo) ...
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bioRxiv - Biochemistry 2023Quote: ... DNA libraries were prepared using NEBNext Ultra II DNA Library Prep Kit (NEB #E7645S), and sequenced by NovaSeq 6000 System.
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bioRxiv - Biochemistry 2023Quote: ... site-directed mutagenesis was performed using Q5 Site-Directed Mutagenesis Kit (New England Biolabs) according to the manufacturer manual ...
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bioRxiv - Genetics 2023Quote: ... and NGS libraries were quantified by qPCR using the NEBNext Library Quant Kit (NEB). Illumina sequencing was performed using the NextSeq platform with automated demultiplexing and adaptor trimming (Illumina).
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bioRxiv - Cell Biology 2022Quote: Point mutations in hYAP1-1δ were generated with Q5 Site-Directed Mutagenesis Kit (NEB) according to the manufacturer’s instructions to synthesise phosphomimic mutants by substituting serine with aspartic acid at two residues ...
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bioRxiv - Cell Biology 2022Quote: ... and ligated into pcDNA5/FRT/TO-N-FLAG-BirA* using Quick Ligation Kit (NEB). Top10 competent E ...
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bioRxiv - Cancer Biology 2023Quote: ... Extraction was performed by MonarchTotal RNA Miniprep kit (New England Biolabs, Ipswich, MA, USA) according to manufacturer extraction to separate RNA molecules shorter and longer than 200 nucleotides in two different fractions ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA was converted to cDNA using LunaScript RT SuperMix Kit (NEB, cat. no. E3010) according to manufacturer specifications ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, E7770). The quality and concentration of libraries were assessed with Bioanalyzer (Agilent ...
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bioRxiv - Genomics 2022Quote: ... the NEBNext Ultra II DNA library prep kit (New England Biolabs, Ipswich, MA, USA) was used according to protocol ...
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bioRxiv - Genomics 2023Quote: ... Samples were further quantified by qPCR using NEBNext Library Quant Kit for Illumina (NEB) and then pooled equimolarly based on estimated concentrations ...
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bioRxiv - Genomics 2022Quote: ... RT-qPCR was performed with Luna Universal One-Step RT-qPCR Kit from NEB on BioRad CFX96 Touch Real-Time PCR Detection System ...
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bioRxiv - Genomics 2023Quote: ... before the library was prepared with the NEBNext RNA Library Prep kit (Biolabs, E7540). Samples were sequenced on a NovaSeq 6000 (Illumina ...
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bioRxiv - Genetics 2023Quote: Libraries were generated using the NEBNext® rRNA Depletion Kit (NEB, cat no. E6310) and UltraTM II Directional RNA Library Prep Kit for Illumina® (NEB ...
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bioRxiv - Immunology 2023Quote: ... The VHH specific sequence was amplified using PCR (Phusion High Fidelity PCR kit, NEB) and correct overhangs were incorporated ...
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bioRxiv - Developmental Biology 2023Quote: ... First-strand cDNA was synthesized using a ProtoScript II cDNA first strand kit (NEB) with 1 μg of total RNA ...
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bioRxiv - Biochemistry 2023Quote: TgTR N147 was mutated to threonine using the Q5 Site-Directed Mutagenesis Kit (NEB) per the manufacture’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Site-directed mutagenesis was performed using the Q5® Site-directed mutagenesis kit (NEB) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... MscS mutants were generated using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs).
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bioRxiv - Microbiology 2023Quote: cDNA libraries were prepared using a NEBNext Ultra II RNA Library Prep Kit (NEB). RNA and cDNA quality and quantity were evaluated by performing a Qubit assay with the TapeStation 4200 system (Agilent Technologies) ...
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bioRxiv - Microbiology 2022Quote: Chosen residues were substituted with alanine using Q5® Site-Directed Mutagenesis Kit (NEB) according to supplier protocol ...
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bioRxiv - Cell Biology 2023Quote: ... using primers synthesized by IDT and a Q5® Site-Directed Mutagenesis Kit (NEB).
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bioRxiv - Molecular Biology 2023Quote: ... and purified using the Monarch® RNA Cleanup Kit (50 μg) (New England Biolabs) according to the adjusted manufacturer’s instructions for the purification of RNA ≥ 15 nt ...
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bioRxiv - Cell Biology 2023Quote: ... Mutations on eGFP-ATP7B were prepared following Q5 Site-Directed Mutagenesis Kit (NEB #E0554) protocol.
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bioRxiv - Immunology 2022Quote: ... cleaned and concentrated 20x using the Monarch PCR & DNA Cleanup Kit (5 µg) (NEB). Further cleanup was done with the E-gel imager system (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... The library kit and type of sequencer were TruSeq Stranded Total RNA (NEB Microbe) and NovaSeq ...
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bioRxiv - Synthetic Biology 2024Quote: ... Recovery products were then purified with the Monarch® PCR & DNA Cleanup Kit (NEB) and eluted in 10 µl of the Monarch® DNA Elution Buffer ...
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bioRxiv - Synthetic Biology 2024Quote: ... The library kit and type of sequencer were TruSeq Stranded Total RNA (NEB Microbe) and NovaSeq ...
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bioRxiv - Genomics 2024Quote: ... The assembly was then purified with the Monarch PCR & DNA cleanup kit (NEB, T1030S), eluted in 6 µL of water ...
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bioRxiv - Developmental Biology 2024Quote: ... Libraries were prepared using the NEBNext Ultra II Directional kit(NEB, Cat no E7760L) with ribodepletion (Qiagen QIAseq FastSelect -rRNA HMR Kit ...