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Citations for New England Biolabs :
3001 - 3050 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... First-strand complementary cDNA was synthesized with LunaScript™ RT SuperMix Kits (New England Biolabs). Quantitative PCR was performed with a CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... 300ng of DNA were used for the library preparation using the Ultra II kit (NEB) for adaptation ...
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bioRxiv - Developmental Biology 2020Quote: ... Libraries were prepared using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) and NEBNext Multiplex Oligos for Illumina (New England Biolabs ...
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bioRxiv - Genomics 2020Quote: ... Alternate allele plasmids were generated via site directed mutagenesis (Q5 SDM kit, New England Biolabs) using the following primers ...
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bioRxiv - Genomics 2020Quote: ... DNA-libraries were multiplexed with NEBNext Multiplex Oligos for Illumina kit (New England Biolabs, UK). According to standard Illumina cluster generation and sequencing protocols ...
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bioRxiv - Evolutionary Biology 2022Quote: ... This was performed as described by the protocol of Illumina sequence library preparation (kit: NEB - M6630 ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA was extracted using the Monarch total RNA Miniprep Kit (New England Biolabs, #T2010) and used for Illumina stranded mRNA library prep ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and cDNA was synthesized using the ProtoScript II First Stand cDNA Synthesis Kit (NEB #E6560S). The experiment was replicated as follows ...
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bioRxiv - Neuroscience 2021Quote: ... Sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Molecular Biology 2021Quote: ... Single step PCR was performed using a One-Taq™ PCR reaction kit (NEB, USA) with 30 amplification cycles and 2 ng of original circular-DNA extract as the DNA template ...
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bioRxiv - Molecular Biology 2021Quote: ... In vitro transcribed RNAs were purified with an RNA Cleanup Kit (New England Biolabs, USA) and quantitated on a Nanodrop (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... HSV-SREBP-1c point mutants were prepared using the Q5 Site-Directed mutagenesis kit (NEB). Two RHBDL4 mutants were produced by PCR using primers containing mutation according to references (Fleig et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... The vector fragments and homology arms were cloned by Gibson Assembly (NEB HiFi Assembly Kit).
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bioRxiv - Neuroscience 2021Quote: The Safb1-ΔRE-HA mutant was generated using the Q5 Site-Directed Mutagenesis Kit (NEB). 5’-overlapping primers were designed and the Safb1-HA vector was amplified excluding the RE region (amino acids 552-708) ...
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bioRxiv - Molecular Biology 2021Quote: ... The abundant rRNAs were depleted from the sample using NEBNext rRNA Depletion Kit (NEB, #E6310). The rRNA-depleted RNAs were then subjected to RNA-seq library construction using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina® (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... psgaDC3XFLAG was obtained by ligating both amplicons using the Gibson Assembly® Cloning Kit (NEB), to replace acaDC with sgaDC ...
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bioRxiv - Molecular Biology 2020Quote: ... Mutations in the pRS315-Rpc160 plasmid were generated using the Gibson Assembly Cloning Kit (NEB) and clones were confirmed by sequencing the entire coding region ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra Directional RNA Library Prep kit (NEB), indexed using NEBNext Multiplex Oligos (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... depleted with the NEBNext rRNA Depletion Kit (Human/Mouse/Rat, NEB, Figures 6 and S6), or not depleted (Figures 2B ...
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bioRxiv - Molecular Biology 2021Quote: ... Libraries were generated with the NEBNext Ultra Directional RNA Library Prep kit for Illumina (NEB) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Purified digestion products were ligated to Esp3I-treated LentiCRISPR-v2 using Gibson assembly kit (NEB) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA extracted by TRIzol-Chloroform or the RNA-Easy kit was treated with DNaseI (NEB) to remove DNA contamination ...
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bioRxiv - Cell Biology 2022Quote: ... we used the NEBNext Ultra II RNA library prep kit for Illumina (New England Biolabs) (9 cycles of amplification) ...
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bioRxiv - Cell Biology 2022Quote: 5’ Phosphorylated RA3 oligonucleotides were adenylated using 5’ DNA Adenylation Kit (New England BioLabs E2610S), by mixing 1 μL of 100 μM of 5’ Phosphorylated RA3 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Total mRNA was isolated using the Magnetic mRNA Isolation Kit (New England Biolabs, Ipswich, MA) and cleaned up using RNeasy MinElute Cleanup Kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: A template-switching 5’ rapid amplification of cDNA ends (5’-RACE) kit (New England BioLabs) was used to map the transcription start site of rflP ...
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bioRxiv - Genomics 2020Quote: ... further verified by qPCR using NEBNext Library Quant Kit for Illumina (New England Biolabs #E7630S) on Stratagene Mx3000P instrument and its profile analysed by Agilent Tape station 2200 ...
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bioRxiv - Genomics 2020Quote: ... The NEBNext Ultra Directional RNA Library Prep Kit for Illumina (cat. # E7420L New England BioLabs) was used to prepare RNA-seq libraries and sequencing was performed on a HiSeq 4000 Illumina genome analyser using 50 paired-end reads ...
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bioRxiv - Genomics 2020Quote: ... Repair template plasmids were assembled using the NEBuilder Hifi DNA Assembly Kit (New England BioLabs) to incorporate homology arms into the loxP + Pmyo-2∷GFP∷unc-54 3’UTR + Prps-27∷neoR∷unc-54 3’UTR + loxP dual-marker selection cassette vector (provided by Dr ...
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bioRxiv - Molecular Biology 2021Quote: ... Libraries were generated with the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) following the manufacturer’s recommendations and sequenced by 1×75 single-reads at Fundación Parque Científico de Madrid.
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bioRxiv - Molecular Biology 2021Quote: ... cDNA libraries were prepared using NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs) according to the manufacturer’s instructions ...
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Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: ... using the Luna Universal One-Step RT-qPCR Kit (New England BioLabs, #E3005L or #E3006E) according to manufacturer’s instructions and then analyzed with QuantStudio Design & Analysis Software v1.5.1 (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... Sequencing libraries were prepared using NEBNext ultra II FS DNA library kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Sequencing libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (NEB E7645) and 200 – 500 base-pair size selection was performed using a Pippin Prep system (Sage Science) ...
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bioRxiv - Genetics 2020Quote: ... and indexing with NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturers’ instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA-seq libraries were prepared using NEBNext ultra directional RNA library prep kit (NEB, E7420L) and sequenced on an Illumina Hiseq 4000 system ...
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bioRxiv - Biochemistry 2020Quote: ... Monarch DNA Gel Extraction kit was used when necessary (New Englands Biolabs, Iswitch, MA, US). Overexpression vectors were the pOtoxLuc for O ...
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bioRxiv - Genomics 2020Quote: ... prepared with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB, #E7103), and sequenced using 150-bp pair-ended sequencing on Illumina platform ...
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bioRxiv - Genetics 2021Quote: ... and NEBNext Ultra II directional RNA library prep kit for Illumina (NEB#E7760, NEB#E7765)” from 250ng of sample ...
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bioRxiv - Genomics 2021Quote: ... rRNA was removed from 3 replicates with NEBNext Bacteria rRNA Depletion Kit (New England Biolabs), while the other 3 were treated with NEBNext Depletion Core Reagent Set using custom probes targeted to Haloferax volcanii rRNA (Martinez-Pastor and Sakrikar ...
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bioRxiv - Genomics 2021Quote: ... Total RNA extraction was carried out using the Monarch RNA Cleanup Kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... and NEBNext Ultra II directional RNA library prep kit for Illumina (NEB#E7760, NEB#E7765)” from 250ng of sample ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Total mRNA was extracted using the Magnetic mRNA Isolation Kit (New England Biolabs, Ipswich, MA) and RNeasy MinElute Cleanup Kit (Qiagen ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, MA, USA) was used to process the samples according to the protocol “NEBNext Ultra II Directional RNA Library Prep Kit for Illumina” ...
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bioRxiv - Biophysics 2021Quote: Point mutations in all constructs were made by using Q5 Site-Directed Mutagenesis Kit (NEB). Primers were designed with NEBase Changer ...
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bioRxiv - Developmental Biology 2020Quote: ... and T7 RNA polymerase from HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB). Cold-anesthetized female mosquitoes were microinjected with 2.0 µg gene-specific dsRNA using a Nanoject II microinjector (Drummond Scientific Company ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA synthesis was carried out by ProtoScript-II first strand cDNA synthesis kit (NEB, USA) with random hexamer primers ...
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bioRxiv - Microbiology 2021Quote: ... pLentiCRISPR-V2 backbone (Sanjana, Shalem and Zhang 2014) using Quick Ligation™ kit (M2200, NEB) and transduced into THP-1 WT cells using Lentiviral particles (Fisch et al ...
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bioRxiv - Microbiology 2020Quote: ... The resulting fragment was cloned using the NEBuilder HiFi DNA assembly kit (NEW ENGLAND BioLabs) into the pMiniMAD2 plasmid previously digested using the restriction sites BamHI and EcoRI ...
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bioRxiv - Biochemistry 2020Quote: ... The RNA was then purified using a Monarch RNA Clean-up Kit (New England BioLabs).