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Citations for New England Biolabs :
2801 - 2850 of 5089 citations for 8 4 Chlorophenylthio 2' O methyladenosine 3' 5' cyclic monophosphate sodium salt since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... 2.5 μL 10 mM MnCl2 and 2 μL Lambda Protein Phosphatase (NEB). The dephosphorylation reaction was allowed to occur at 30 °C overnight ...
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bioRxiv - Microbiology 2024Quote: ... cenocepacia K56-2 genome using Q5 polymerase with high GC buffer (NEB) and primers 3107 and 3108 (Supplementary Table 10 ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.2 mM dCTP and 2 U of terminal transferase (New England Biolabs). The mix was incubated at 37°C for 30 min ...
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bioRxiv - Biophysics 2019Quote: ... the 5’ ends of the PCR products were phosphorylated by T4 polynucleotide kinase (NEB), the products were purified and the free ends of the plasmid re-ligated by T4 DNA ligase (NEB) ...
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bioRxiv - Developmental Biology 2020Quote: ... 3mM MgCl2) for 5 min before digesting with 0.2U/ml of DNase I (NEB). Embryos were then fixed in 4% PFA ...
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bioRxiv - Developmental Biology 2021Quote: ... To each of those tubes 5 μl of 10x CutSmart Buffer (New England Biolabs) and 2 μl of DpnI restriction enzyme (New England Biolabs ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was resuspended and treated with 7.5 units (1.5 µl) RNA 5′ pyrophosphohydrolase (NEB) for 1 h at 37°C in a total volume of 20 µl ...
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bioRxiv - Cell Biology 2022Quote: ... and then unligated linker was digested away with 25 U Yeast 5’-deadenylase (NEB) and 5 U RecJ exonuclease (Epicentre) ...
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bioRxiv - Microbiology 2019Quote: ... Monarch® PCR & DNA Cleanup Kit (5 μg) (New England Biolabs GmbH; Ipswich, USA), or innuPREP DOUBLEpure Kit (Analytik Jena ...
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bioRxiv - Immunology 2019Quote: ... PCR product was cloned into 5-alpha competent bacteria (New England Biolabs, cat. #C2987) using a TOPO TA cloning kit (Thermo Fischer Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... dynein-coated beads were labeled with 5 µM SNAP-Cell-TMR (New England Biolabs) in the column for 10 min at room temperature and unbound dye was removed with a 300 mL wash with TEV buffer at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Samples (20 μL) were loaded in DNA loading buffer (5 μL; New England Biolabs) and gels were run at 60 V for 120 mins.
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bioRxiv - Molecular Biology 2020Quote: ... RNA was treated with RppH (RNA 5’ Pyrophosphohydrolase, Art. No. M0356S, New England Biolabs) to dephosphorylate small RNAs and specifically enrich for 22G RNAs ...
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bioRxiv - Molecular Biology 2019Quote: ... The reannealed fragments were treated with 5 units of T7 endonuclease 1 (NEB, #M0302S) at 37°C for 25 min and immediately separated with 7% polyacrylamide gel electrophoresis.
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bioRxiv - Bioengineering 2019Quote: Phosphorylation master mix (5x): 1 mM Adenosine 5’-Triphosphate (ATP, New England Biolabs, NEB), 0.1 U/µL T4 Polynucleotide Kinase (T4 PNK ...
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bioRxiv - Bioengineering 2019Quote: Phosphorylation master mix (5x): 1 mM Adenosine 5’-Triphosphate (ATP, New England Biolabs, NEB), 0.1 U/µL T4 Polynucleotide Kinase (T4 PNK ...
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bioRxiv - Genomics 2021Quote: ... 5 µL of 5X Phusion HF reaction buffer (all from New England Biolabs, USA), 0.5 µL of 10 mM premixed dNTPs and 0.5 µL of gDNA ...
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bioRxiv - Cell Biology 2021Quote: ... dynein-coated beads were labeled with 5 µM SNAP-Cell-TMR (New England Biolabs) in the column for 10 min at room temperature and unbound dye was removed with a 300 mL wash with TEV buffer at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: 5-10 μg RNA was diluted in 2x RNA loading dye (New England Biolabs) and size separated on 7 M Urea/15% polyacrylamide/0.5×TBE gels by gel electrophoresis ...
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bioRxiv - Biophysics 2020Quote: ... the cells were first labelled with 5 µM biotin-conjugated SNAP-tag substrate (NEB) in tissue culture medium ...
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bioRxiv - Cell Biology 2022Quote: ... dynein-bound beads were mixed with 5 µM SNAP-Cell-TMR (New England Biolabs) for 10 min at RT ...
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bioRxiv - Microbiology 2022Quote: Escherichia coli DNA adenine methyltransferase (dam)+/dcm+ (NEB 5-alpha competent E. coli, #C2987) and dam−/dcm− (dam−/dcm− competent E ...
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bioRxiv - Immunology 2020Quote: ... about 5 μg of the genomic DNA was digested with HaeIII (New England Biolabs) and purified using phenol:chloroform extraction followed by ethanol precipitation ...
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bioRxiv - Microbiology 2021Quote: ... 5 μl of 2X Blunt/TA Ligase master mix (New England Biolabs, cat#M0367S), and milliQ water up to 10 μl total volume ...
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bioRxiv - Molecular Biology 2020Quote: ... which was treated with 5 units of T7E1 (New England Biolabs, Ipswich,MA, USA) for 20 minutes at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... the RNP injection mix [5 µM EnGen® Spy Cas9 NLS (NEB Cat# M0646T) mixed with 2.5 µM of each gRNA and Cas9 buffer] was freshly prepared ...
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bioRxiv - Zoology 2021Quote: ... we split the library and ran 5 high-fidelity Phusion (New England BioLabs, #M0530) PCRs for 12 cycles on a SimpliAmp thermal cycler (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... 5’ adaptor ligation using T4 RNA Ligase 1 (New England Biolabs, Ipswich, MA, M0204L). Generation of cDNA was done by using Superscript III Reverse Transcriptase (Life Technologies ...
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bioRxiv - Developmental Biology 2019Quote: ... 5 µl of the whole transcriptome cDNA was PCR amplified using Phusion polymerase (NEB) and 10XPCR1_F (CTACACGACGCTCTT CCGATCT ...
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bioRxiv - Systems Biology 2019Quote: ... The 5’-ends of bound RNAs were radiolabelled with 30U T4 PNK (NEB, M0201L) and 3μl 32P-γATP (1.1µCi ...
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bioRxiv - Cancer Biology 2020Quote: ... 400 ng of RNA was digested by 5 U of RppH (New England Biolabs) for 2 h at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... After incubation with 5‘-phosphate-dependent exoribonuclease XRN-1 (New England BioLabs, Inc., USA), samples were treated with RNA 5‘ polyphosphatase (Lucigen) ...
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bioRxiv - Genomics 2020Quote: ... incubated for 5 minutes and magnetically separated using a Magnetic Separation Rack (NEB #S1509S). Three 1 mL 70% ethanol washes are performed on the Eppendorf tubes ...
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bioRxiv - Biochemistry 2021Quote: ... Control samples containing only RNA were treated with RNA 5’ Pyrophosphohydrolase (RppH, NEB, M0356S) to convert 5’-PPP RNA into 5’-P RNA ...
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bioRxiv - Microbiology 2021Quote: ... dsRNA ladders and a 5’ Fluorescein-labeled RNA (300 nt) were provided by NEB and used as substrates for RNase I and RNase III activity assays ...
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bioRxiv - Cell Biology 2021Quote: ... dynein-coated beads were labeled with 5 μM SNAP-Cell-TMR (New England Biolabs) in the column for 10 min at room temperature and unbound dye was removed with a 300 mL wash with TEV buffer at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... coli transformations were performed using NEB 5-alpha chemically competent cells (New England BioLabs). E ...
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bioRxiv - Microbiology 2021Quote: ... 5’ RACE products were generated with Template Switching RT Enzyme Mix (New England Biolabs) using anchored poly(dT)23 and TSO (GCT AAT CAT TGC AAG CAG TGG TAT CAA CGC AGA GTA CAT rGrGrG ...
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bioRxiv - Molecular Biology 2021Quote: ... in the presence of 5 U of Quick-CIP alkaline phosphatase (New England Biolabs). After dialysis ...
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bioRxiv - Genomics 2022Quote: ... Dangling ends were removed by a 5 min incubation with Exonuclease III (NEB #0206) at 37 °C and biotin enrichment was done using 20 ul Dynabeads™ MyOne™ Streptavidin C1 beads (Invitrogen #65001) ...
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bioRxiv - Molecular Biology 2022Quote: ... The linkers that were pre-adenylated using a 5’ DNA adenylation mix (NEB, #E2610L) were ligated to dephosphorylated RNAs using T4 truncated RNA ligase 2 (K227Q ...
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bioRxiv - Microbiology 2024Quote: ... DNase-treated total RNA was further treated with RNA 5’ Pyrophosphohydrolase (New England Biolabs) to remove possible pyrophosphate from the 5’ end ...
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bioRxiv - Molecular Biology 2024Quote: ... and 5′ end labelled using γ32P UTP (Perking Elmer) and T4 polynucleotide kinase (NEB) following manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... Ligation products were transformed into either 5-alpha or 10-beta electrocompetent cells (NEB) and grown in liquid LB-Amp cultures ...
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bioRxiv - Bioengineering 2024Quote: The 5’ RACE protocol using the template-switching reverse transcriptase enzyme mix from NEB was executed following the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2024Quote: ... Dephosphorylation of DNA ends was performed by addition of 5 μl rSAP (NEB, #M0203) and incubation at 37°C for 45 min ...
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bioRxiv - Pathology 2024Quote: ... 5 min ice) and plasmids were extracted using the Monarch Plasmid Miniprep kit (NEB) following manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... 5% (v/v) DMSO and 0.5 U Phusion High-Fidelity DNA polymerase (NEB, M0530S). Cycling was performed using the following thermocycler settings ...
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bioRxiv - Genetics 2023Quote: ... along with an additional 5 µL 2x Gibson Assembly Master Mix (NEB Cat#E2611L). The reaction mixture was incubated at 50°C for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... All the produced constructs were propagated in competent cells (5-alpha Competent E.coli; NEB) and isolated by NucleoSpin Plasmid (TaKaRa) ...