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Citations for New England Biolabs :
2801 - 2850 of 3709 citations for 1 1' Diethyl 4 4' bipyridinium dibromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and TE buffer, DNA was reverse crosslinked by incubating beads in Elution buffer (1% SDS, 0.1M NaHCO3 and Proteinase K (NEB)) ...
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bioRxiv - Molecular Biology 2023Quote: ... A 5′ adapter (rArCrArCrUrCrUrUrUrCrCrCrUrArCrArCrGrArCrGrCrUrCrUrUrCrCrGrArUrCrU, IDT) was then ligated to RNAs to the product using T4 RNA ligase 1 (NEB) for 4 hours at 15°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µL of RNase H (5,000 U/mL) (New England Biolabs, Fig. 2f and Extended Data Fig. 2c,d) were treated with 1.5 of 10× RNase H buffer in the sample by adjusting with Milli-Q H2O to total 15 µL ...
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bioRxiv - Neuroscience 2024Quote: ... After ligation of the 5’-RNA adapter (5’-GUUCAGAGUUCUACAGUCCGACGAUC-3’) using T4 RNA ligase 1 (New England Biolabs; M0204S), the RT primer was annealed to the 3’-adapter ...
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bioRxiv - Microbiology 2024Quote: ... the denatured dsRNA was mixed with the DNA adaptor for adaptor ligation using T4 RNA ligase 1 (M0204, NEB). Subsequently ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.01 pmol (2:1 molar ratio of insert:backbone) library and 2.5 uL NEBuilder HiFi DNA Assembly Master Mix (NEB E2621) and incubated at 50 °C for 60 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg non-methylated NEAT1_1 IVT product was radio-labelled with radioactive labelling mix (1 µL 10x PNK buffer, NEB, 1 µL NEAT1_1 IVT product ...
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bioRxiv - Molecular Biology 2024Quote: ... the plug was incubated in 160 μL of 1× NEBuffer 3.1 buffer containing 160 units of Bgl II (New England Biolabs) overnight at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... the total RNA of each sample (50 μg) was treated with 1 μL of polynucleotide kinase (New England Biolabs) in 50 μL of total solution at 37℃ for 6 h ...
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bioRxiv - Microbiology 2024Quote: Test PCRs on cDNA were performed in a 50 µL scale using 1.0 µL precipitated cDNA in the presence of 1 x High-Fidelity PCR MasterMix (NEB), 2 µM forward primer with native barcode and 2 µM reverse primer with native barcode ...
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bioRxiv - Biophysics 2024Quote: ... Reactions were dialyzed with water on silica membranes (0.025-μm pores) for 1 h before transformed into DH10B cells (New England Biolabs). Library sizes of at least 100,000 colony-forming units (cfu ...
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bioRxiv - Developmental Biology 2023Quote: ... mixed in a ratio 1:3 (insert:backbone plasmid) and ligated with T4 ligase according to manufacturer’s instructions (Quick Ligation kit, NEB M2200). The ligation reaction was transformed into competent TOP-10 bacteria and plated on agarose plates with Ampicillin ...
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bioRxiv - Biochemistry 2024Quote: ... 0.5 μM fluorescently tagged 3’ adapter (MultiplexDX) were ligated with T4 Rnl2(1–249)K227Q (M0351, New England Biolabs) overnight at 4°C and washed three times with PNK/ligation buffer ...
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bioRxiv - Cell Biology 2024Quote: ... approximately 1 μg of gDNA each sample was subject to bisulfite conversion for shotgun library construction (NEB Ultra II) and Illumina HiSeqX PE150 sequencing ...
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bioRxiv - Immunology 2024Quote: ... the drh-1(2CARD) sequence was deleted from pET770 using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Primers LEB003 and LEB004 were used to amplify around the drh-1(2CARD ...
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bioRxiv - Genomics 2024Quote: ... 500 µl lysed cells were then transferred to a tube containing 4.5 ml digestion buffer (1% Triton X-100, 1x CutSmart buffer (New England Biolabs)) ...
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bioRxiv - Immunology 2024Quote: ... top and bottom strand oligos (1 µl of 100 µM each) were phosphorylated with T4 PNK (New England Biolabs) for 30 minutes at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... the cap-1 structure was added using the vaccinia virus capping enzyme and 2L-O-methyltransferase (New England Biolabs). The mRNA was purified ...
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bioRxiv - Microbiology 2024Quote: Final PCRs on cDNAs were performed in a 100 µL scale using 2.0 µL precipitated cDNA in the presence of 1 x High-Fidelity PCR MasterMix (NEB), 2 µM forward primer with native barcode and 2 µM reverse primer with native barcode (e.g BC1 fwd and rev ...
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bioRxiv - Microbiology 2024Quote: ... An aliquot of 1 A260 unit of ribosome-bound mRNA fraction was digested with 800 units Micrococcal nuclease (NEB) for 20 min at 25°C ...
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bioRxiv - Biochemistry 2024Quote: The 1D4 purified receptor was mixed with purified jsGiq or hGi1 heterotrimer at a molar ratio of 1:1.25 and supplemented with apyrase (New England Biolabs) (25 mU/mL ...
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bioRxiv - Biochemistry 2024Quote: ... Cleavage reactions were incubated at 37°C for 1 hour and subsequently worked up with 0.2 mg/mL RNase A (New England Biolabs) and 4 units of Proteinase K (New England Biolabs).
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bioRxiv - Biophysics 2024Quote: ... was obtained from Addgene (#31207) and subsequently inserted into a pGEX-6p-1 vector (Cytiva, 28954648) using Gibson Assembly (New England Biolabs, E2611L). The resulting construct was expressed in Escherichia coli BL21(DE3 ...
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bioRxiv - Microbiology 2023Quote: ... xanthus cells grown on 1.5% agar supplemented with 1% CTT broth was extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs). Briefly ...
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bioRxiv - Genetics 2023Quote: ... The remaining beads were incubated with 1 ul RNasin (Fisher) and 0.5 µl of 20 mg/ml Proteinase K (NEB) at 42°C and 1200 rpm for 15 minutes in a thermomixer ...
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bioRxiv - Biochemistry 2023Quote: Trx-linker concatemers (1 mg/mL) were incubated with 50,000 units of the catalytic subunit of cAMP-dependent protein kinase (PKA) (NEB)—which recognizes the RRAS motif within the central linker of the Trx-linker nonamer—in protein kinase buffer (50 mM Tris HCl ...
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bioRxiv - Biophysics 2023Quote: ... and ScFv16 at room temperature for 2h in the presence of 25mU ml-1 apyrase (NEB, Cat. no: M0398L) and either hC5a or C5apep for complex formation ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 mM MgCl2, 1% CHAPS,2.5 mM DTT, 50 µg/mL cycloheximide, 20 U RNase inhibitor murine, New England BioLabs, EDTA-free protease inhibitor cocktail ...
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bioRxiv - Cell Biology 2023Quote: ... elegans osgn-1 (F30B5.4a, WormBase WS265) and human OSGIN1 were amplified by PCR using Phusion DNA polymerase (NEB, M0530L), according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... The reporter HLC plasmid (20ng μl-1) was digested by 0.5 U I-Sce enzyme in its adequate digestion buffer (NEB) for 30’ at 37°C prior to injections ...
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bioRxiv - Biochemistry 2023Quote: ... In a 50 μL reaction 1 μg of backbone plasmid (starting_eGPARK2iM) was digested at 37 °C for 1 hour with MluI-HF and EcoRI-HF (New England Biolabs), then heat-inactivated at 65 °C for 20 minutes ...
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bioRxiv - Biochemistry 2023Quote: The library was ligated to the barcode oligonucleotide at a 7:1 oligo:library ratio overnight at 16 °C with T4 DNA ligase (New England Biolabs). A no-insert negative control was also ligated overnight using identical conditions ...
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bioRxiv - Physiology 2023Quote: ... Cleavage of GS linkers from MBP was done in column using 1 unit of TEV protease (Catalog P8112S, NEB) for every 2 µg of fusion protein in 1X TEV buffer (Catalog P8112S ...
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bioRxiv - Bioengineering 2023Quote: ... NEB RNAPol reaction buffer (M0251S; 10X) and yeast inorganic pyrophosphatase (YIPP; M2403S; 0.1 U μl−1) were purchased from New England Biolabs (NEB). RNase H was purchased from ThermoFisher Scientific (EN0201 ...
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bioRxiv - Plant Biology 2023Quote: Mutants in the CG-1 domain were created using the Q5 site-directed mutagenesis kit (New England Biolabs, USA) as per manufacturer instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR amplification was done in a 25µl reaction with 1 unit of NEB Taq DNA polymerase (NEB Catalog# M0273S), 1x Standard Taq Buffer ...
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bioRxiv - Molecular Biology 2023Quote: For DNA-RNA hybrid IP (DRIP) non-crosslinked lysates were incubated (1 h, 37°C) with 10U RNaseH (NEB) prior to immunoselection ...
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bioRxiv - Molecular Biology 2023Quote: ... 1.0-3.0 μg of DNA was used for a ligation reaction in a volume of 1 mL using 1.0 μL of 2000 U/μL T4 DNA Ligase (NEB) for 3C experiments ...
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bioRxiv - Immunology 2023Quote: ... were single-cell sorted into 5 µl 1% (v/v) Nonidet P40 Substitute, Tris-HCl (20 mM, pH 8.0) containing 5 U murine RNase inhibitor (NEB) using a FACSAriaIII instrument (BD) ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μg of immunopurified GST-tagged WRN fragment was phosphorylated in vitro by Casein Kinase II (New England Biolabs) in the presence of 1X NEBuffer (50mM Tris-HCl ...
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bioRxiv - Genomics 2023Quote: ... were mixed in 5 μL of 1x T4 DNA Ligase buffer (50 mM Tris-HCl pH 7.5, 10 mM MgCl2, 1 mM ATP, 10 mM Dithiothreitol; New England Biolabs) at a final concentration of 80 μM each and annealed by heating at 65 °C for 15 min ...
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bioRxiv - Bioengineering 2023Quote: ... mRNA was isolated from ~1 μg of total RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490). RNA integrity was assessed using the RNA 6000 Pico Kit for Bioanalyzer (Agilent Technologies 5067-1513) ...
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bioRxiv - Immunology 2022Quote: ... Then 1 μg of vaccine mRNA was ligated to 20 pmols RA3_7N adaptor: 5rApp/CTGACNNNNNNNTGGAATTCTCGGGTGCCAAGG/3ddC with 10U of T4 KQ227 RNA ligase 1 (#M0204S NEB) in the presence of 20U RNase OUT (#10777019 ...
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bioRxiv - Cell Biology 2022Quote: ... Tracheal chitin was stained with 505 star conjugated chitin-binding probe (NEB, Frankfurt/M, Germany, used at 1:300). Nuclei were stained with 4’,6-Diamidino-2-Phenylindole ...
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bioRxiv - Biochemistry 2022Quote: ... and 10 μM GDP and incubated for 1 h at room temperature prior to adding 0.25 units of Apyrase (NEB) and incubation for a further 1 h at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... reactions were carried out in a total of 25 μL containing 1 X Isothermal Amplification Buffer (New England Biolabs), 5 mM MgSO4 ...
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bioRxiv - Microbiology 2023Quote: ... 0.5 µL of T4 DNA Ligase and 1 µL of 10 mM ATP (all reagents from New England Biolabs, Ipswich ...
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bioRxiv - Microbiology 2023Quote: ... 20 μL of the reaction product was treated with 1 μL of CIP or nuclease P1 (New England BioLabs) at 37 °C for 1 h and inactivated at 80 °C for 10 min prior to centrifugation and analysis by HPLC.
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Microhomology-Mediated Circular DNA Formation from Oligonucleosomal Fragments During SpermatogenesisbioRxiv - Genomics 2023Quote: ... Column-bound DNA was eluted in TE buffer (10 mM Tris-Cl, pH 8.0; 1 mM EDTA, pH 8.0) and treated with AsiSI (NEB, R0630S) and PacI (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... To obtain dephosphorylated TOP2B used for in vitro kinase assay and mass spectrometry the YFP column incubated twice for 15 min at room temperature in wash buffer supplemented with 0.1mM MnCl2 and 5 units mL-1 calf intestinal phosphatase (NEB), 400 units mL-1 lambda protein phosphatase (NEB) ...