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Citations for New England Biolabs :
2951 - 3000 of 3709 citations for 1 1' Diethyl 4 4' bipyridinium dibromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The RNA fragments were ligated with 3’-barcoded adaptors at 22 °C for 1 h and 30 min using T4 RNA Ligase (NEB). Barcoded RNA samples were pooled together ...
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bioRxiv - Microbiology 2020Quote: ... Cell pellets were resuspended in 1 ml of iCLIP lysis buffer B (same as buffer A but with 1% v/v NP-40 and 11 μl of Murine RNase inhibitor (NEB) per 1 ml ...
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bioRxiv - Systems Biology 2020Quote: ... We neutralized the tagmentation activity of Enzyme 1 by immediately cleaning the reaction with the Monarch PCR & DNA Cleanup Kit (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... a 2.4-kb PCR fragment spanning Rv3377c-Rv3378c was generated using primers BamHI-Rv3377c-Rv3378c-F and HindIII-Rv3377c-Rv3378c-R (Sup. Table 1) using high-fidelity Phusion DNA polymerase (New England Biolabs). The fragment was subsequently digested with BamHI and HindIII (all restriction enzymes from New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... The hygR insert and the inverse PCR product of the pEX18Ap backbone were mixed at a 5:1 ratio and ligated for 30 minutes at 50°C with New England Biolabs (NEB) Gibson Assembly ® Cloning Kit to create pEX18HygB ...
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bioRxiv - Developmental Biology 2021Quote: ... Pmex-5::PH-GFP-cyk-1(700-1437)::tbb-2 3’UTR in the pCFJ150 backbone [89] was made using HiFi cloning (New England Biolabs). Notably ...
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bioRxiv - Microbiology 2021Quote: ... were PCR-amplified using primers that contained restriction sites for EcoRI and SacI (Table S5, primers No. 1-6) and the high-fidelity polymerase Q5 (New England Biolabs) according to vendor’s manual ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cDNA was synthesized from 1 μg of total RNA using M-MuLV Reverse Transcriptase and Random Primers 6 (both New England Biolabs) at 42 °C for 60 min and diluted in 1:4 ratio by PCR grade water ...
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bioRxiv - Microbiology 2021Quote: ... which were incubated with 1 μg/ml full-length S protein with His tag that had been pretreated with or without FXa (P8010L, NEB) for 2 hours at room temperature ...
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bioRxiv - Plant Biology 2021Quote: ... Supplementary Table 1) were hybridized by slow cooling from 95-25°C and then phosphorylated using T4 Polynucleotide Kinase (NEB). The digested plasmid and the hybridized oligonucleotides were ligated using T4 Ligase (NEB ...
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bioRxiv - Plant Biology 2020Quote: ... for sgRNA-LecRK-I.1 and pMR218 (L5-L2) for sgRNA-LecRK-I.6 via a cut-ligation reaction with BbsI (New England Biolabs) and T4 DNA ligase (New England Biolabs) ...
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bioRxiv - Molecular Biology 2021Quote: ... The gene fragment was amplified from approximately 30ng of DNA in each sample (primers in Supplementary Table 1) using a high-fidelity polymerase (NEB Q5, New England Biolabs)[27] and confirmed by 1% agarose gel electrophoresis ...
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bioRxiv - Molecular Biology 2021Quote: ... were cloned into pEGFP-C2 or pGEX5X.1 vector and transformed into NEB 10-beta competent E.Coli (New England Biolabs # C3019H). Mutagenesis to generate PARP1 ...
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bioRxiv - Microbiology 2020Quote: ... 3-5 μg of RNAse H treated RNA was circularized using T4 RNA ligase 1 (ssRNA Ligase, New England Biolabs), RNA was extracted with phenol/chloroform approach and ethanol precipitated ...
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bioRxiv - Cell Biology 2020Quote: ... the CometChip was washed three times with PBS before being incubated for 1 h at RT in FPG (M0240S New England Biolabs) diluted 1:104 in PBS ...
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bioRxiv - Biophysics 2020Quote: ... The lysate was cleared from cell debris by centrifugation at 50000 g for 1 hour followed by incubation with Amylose resin (NEB) for 1 hour ...
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bioRxiv - Biophysics 2022Quote: ... Then 50-fold molar excess of oligo 2 was added to the mixture along with an additional 1 µL of T4 DNA ligase and T4 DNA ligase buffer (NEB) with ATP adjusting for the change in volume and allowed to incubate at room temperature for 30 minutes ...
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bioRxiv - Synthetic Biology 2022Quote: ... αlvus tRNAPyl (Ma-tRNAPyl)35 was prepared by annealing and extending the ssDNA oligonucleotides Ma-PylT-F and Ma-PylT-R (2 mM, Supplementary Table 1) using OneTaq 2x Master Mix (NEB). The annealing and extension used the following protocol on a thermocycler (BioRad C1000 Touch™) ...
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bioRxiv - Genomics 2022Quote: ... The TdT reation was prepared using 4 μL of the resulting elutant and 5 μL of ice-cold TdT mix (standard Quartz-seq2 condition: 1× Thermopol buffer [New England Biolabs] ...
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bioRxiv - Biochemistry 2022Quote: We assembled mutant libraries by combining the linearized sensor backbone with each oligo subpool at a molar ratio of 1:5 using Golden Gate Assembly Kit (New England Biolabs; 37 °C for 5 min and 60 °C for 5 min ...
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bioRxiv - Molecular Biology 2022Quote: The regions of interest (supplementary Table 1) were PCR amplified (supplementary table 3) from the extracted DNA using Q5 high-fidelity DNA polymerase (NEB). Of the primers used in the PCRs (supplementary table 2 ...
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bioRxiv - Immunology 2022Quote: ... The plasmid at 10-25 ng/μl concentration together with 1 μl I-SceI meganuclease and NEB buffer (NewEngland BioLabs) were co-injected into the blastomere at one-cell stage embryos ...
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bioRxiv - Synthetic Biology 2022Quote: ... The reactions were done using 150 ng of each part of the TU and either used the same recipe as level 1 or by using the NEB Golden Gate Assembly Mix (New England Biolabs) according to the manufacturer’s instruction ...
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bioRxiv - Physiology 2022Quote: ... 1 L of diluted extracts were incubated with 9 ml of packed amylose resin (catalog no. E8021S, New England Biolabs) and incubated at 4°C for 2h with gentle rotation ...
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bioRxiv - Biochemistry 2022Quote: ... D-loops were deproteinized by adding 2 μL of 5% lithium dodecyl sulfate and 1 μL of 20 mg/mL proteinase K (New England Biolabs), and incubating the mixture at 37°C for 15 min ...
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bioRxiv - Biochemistry 2022Quote: ... a ~1-1.5Kb fragment that included guide-RNA target sites was PCR amplified using Q5 High-Fidelity DNA Polymerase (NEB; M0491). Primers were designed using the NCBI Primer Blast tool and are documented in the key resources table ...
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bioRxiv - Biochemistry 2022Quote: ... accordingly to manufacturer’s instructions and 1 μg of RNA was reverse-transcribed into cDNA using M-MuLV reverse transcriptase (New England Biolabs) and random hexamers ...
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bioRxiv - Developmental Biology 2022Quote: ... and T492 were created through PCR mutagenesis of an intron-less version of pHC329 using the oligonucleotides (#1-14) and HiFi assembly (NEB) (Fig ...
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bioRxiv - Biophysics 2022Quote: ... The DNA fragment was amplified by PCR by inserting a C-terminal 6x His tag and cloned into the pETDUET-1 vector by Hi-Fi DNA assembly (NEB).
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bioRxiv - Microbiology 2022Quote: ... Individual fragments were assembled by Gibson assembly at 50°C for 1 h in a total reaction volume of 20 µl (NEBuilder HiFi DNA Assembly Cloning Kit, New England Biolabs), thereby adding 6x His-tag coding sequences to the 3’ end of ply007 ...
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bioRxiv - Biochemistry 2022Quote: ... To seventeen microliters of the eluates were added 2 μL of 10x Nucleoside Digestion Mix reaction buffer and 1 μL of Nucleoside Digestion Mix (New England Biolabs). Reactions were incubated at 37 °C for 1 hour ...
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bioRxiv - Biochemistry 2022Quote: ... Reactions were performed at 80 °C for 60 minutes and terminated by the addition of 1 μL of Proteinase K (NEB). Cleavage fragments from pre-linearized substrates were purified using paramagnetic beads and quantified and analyzed as described above ...
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bioRxiv - Biochemistry 2022Quote: ... with 0.1 pmol of ~60bp ssDNA oligo (either oBK9102 or oBK9104) with 10 μL NEBuilder HiFi DNA Assembly Master Mix (NEB) in a final volume of 20 μL ...
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bioRxiv - Microbiology 2022Quote: ... and a subpart of it encoding specifically the PAS domain (amino acids 1-138) the corresponding coding sequence was amplified by PCR using the Phusion DNA polymerase (New England Biolabs) and cloned between the NdeI and BamHI sites ...
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bioRxiv - Biophysics 2022Quote: ... The SETD3 V266D mutant was introduced into EcoRV-digested pLenti-CMV-Puro-Dest (w118-1) by Gibson Assembly (New England Biolabs). The SETD3 V266D construct was then used as a template to sequentially incorporate Q257A and Y288P mutations by Phusion site-directed mutagenesis using the following primer pairs ...
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bioRxiv - Biophysics 2022Quote: ... This SETD3 mutant was introduced into EcoRV-digested pLenti-CMV-Puro-Dest (w118-1) by Gibson Assembly (New England Biolabs).
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bioRxiv - Biophysics 2022Quote: ... The agarose was digested by 1 hour incubation at 42 °C with 2 units of beta-agarase (M0392, New England Biolabs). After this stage ...
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bioRxiv - Biophysics 2022Quote: ... The SETD3 G361R mutant was introduced into EcoRV-digested pLenti-CMV-Puro-Dest (w118-1) by Gibson Assembly (New England Biolabs). The SETD3 G361R construct was then used as a template to incorporate F409A or F409A and N413A mutations by Phusion site-directed mutagenesis using the following primer pairs ...
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bioRxiv - Plant Biology 2022Quote: ... The vector and insert DNA fragments were assembled in a ratio of 1:5 using NEBuilder HiFi DNA Assembly (E5520S, New England Biolabs). The gl2L480P ...
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bioRxiv - Cancer Biology 2022Quote: ... A minimum of 1 μg (20 ng/μl) of high-quality total RNA (extracted using Monarch Total RNA Miniprep Kit, NEB) was supplied for sequencing ...
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bioRxiv - Cell Biology 2022Quote: INS-1 832/3 EV and g1-2 cells transiently expressing the SNAP-GLP-1R were labelled at 37°C with 1 μM of SNAP-Surface 649 fluorescent substrate (S9159S, New England Biolabs) in full media prior to treatment with 100 nM exendin-4 or vehicle for 3 hours ...
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bioRxiv - Developmental Biology 2021Quote: ... pFastBac dual vector containing the sequence for the N-terminally 6xHis-tagged human Naa15 and truncated human Naa10 (residues 1-160) sequences was digested using KpnI-HF (NEB) and XmaI (NEB ...
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bioRxiv - Developmental Biology 2021Quote: Subcloning Both full-length and truncated (1-160) mouse Naa12 were amplified from the pMAL-c5x Naa12 plasmid using Q5 HF Master Mix (NEB), AAAACCCGGGTATGAACATCCGCCGGGCTCGGC as the forward primer ...
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bioRxiv - Developmental Biology 2020Quote: ... The supernatant was removed using a magnetic separator and washed once with 1 mL NEBuffer 2 and resuspended in 115 mL of Ligation reaction with Quick Ligase buffer (NEB), 6,000 U of Quick Ligase (NEB ...
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bioRxiv - Developmental Biology 2020Quote: ... cDNAs were diluted 1:20 and then qPCR was carried out in triplicate using the Luna Universal qPCR Master Mix (New England Biolabs). 3 primer sets targeted different exon-exon junctions in the lmo7a coding region and primers targeting rps13a as the control housekeeping gene were used for normalization ...
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bioRxiv - Microbiology 2020Quote: ... RT-PCR product sizes were analyzed in comparison to migration of markers in 100 bp and 1 kb ladders (New England Biolabs).
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bioRxiv - Cancer Biology 2020Quote: ... Coverslips were treated with RNase A (Fermentas) for 1 hour at 37°C and where indicated with RNase H (NEB) for 24 hours at 37°C prior to blocking ...
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bioRxiv - Cell Biology 2021Quote: ... COS-7 cell lysates from FLAG-ACBD4/5 (mFFAT) expressing cells were treated for 1 h with λPP (New England BioLabs) as described above ...
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bioRxiv - Microbiology 2020Quote: ... Dephosphorylated RNA-protein complexes were then rinsed once with NT2 buffer and 3’-end ligated with T4 RNA Ligase 1 (NEB) overnight in an Eppendorf Thermomixer at 16°C ...
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bioRxiv - Genomics 2020Quote: ... Methylation reactions were performed in a 100 uL reaction with Methylation Reaction buffer (1X CutSmart Buffer,1 mM S-adenosyl-methionine (SAM, New England Biolabs)) and incubated with 2.5 uL EcoGII at 37°C for 30 minutes ...