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Citations for New England Biolabs :
2651 - 2700 of 3709 citations for 1 1' Diethyl 4 4' bipyridinium dibromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... The PCR was performed in a total volume of 50 µl containing 1× HF buffer (New England BioLabs), 1 µl dNTP Mix (New England BioLabs) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μg total RNA was reverse transcribed with 50 ng random hexamers using M-MuLV reverse transcriptase (NEB). 1/20th of the resulting cDNA was used then analyzed by qRT-PCR using GoTaq qPCR master mix (Promega) ...
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bioRxiv - Cell Biology 2024Quote: ... The medium was then replaced with a fresh imaging medium containing 1 µM SNAP Cell Block (NEB, S9106S) to prevent the binding of residual SNAP dye to newly synthesized SNAP-tagged proteins ...
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bioRxiv - Microbiology 2024Quote: In vitro transcribed CHIKV RNA (nt’s 1-337) was 5’ dephosphorylated using Quick CIP according to the manufacturer’s instructions (NEB) before purification using an RNA Clean & Concentrator column (Zymo Research) ...
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bioRxiv - Molecular Biology 2024Quote: ... The RNA adaptor KS_5RACE_linker was ligated to the 5ʹ end of the RNA by incubating with T4 RNA ligase 1 (NEB) in 1 mM ATP and 15% PEG 8000 at 25 °C for 3 h ...
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bioRxiv - Genomics 2024Quote: ... cDNA was treated with 1 μL each of RNase A and RNase H (NEB M0297S and ThermoFisher, EN0531) for 30 min at 37°C ...
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bioRxiv - Genomics 2024Quote: ... were assembled by Gibson assembly using a 2:1 insert:vector ratio with Gibson Assembly Master Mix (NEB E2611S). Assemblies were transformed into NEB 5-alpha E.coli competent cells and single colonies were picked and sequence validated by Sanger sequencing ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1-5 µL of lysate was screened using 25 µL Taq DNA Polymerase with ThermoPol Buffer (NEB, M0267L) reactions following the manufacturer’s protocol with 500 nM primers (Table 2) ...
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bioRxiv - Genomics 2024Quote: ... Chimeric ligation was then performed on beads at room temperature for 1 hour with T4 RNA ligase (NEB) without adapters ...
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bioRxiv - Cell Biology 2024Quote: ... and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1, NEB, E7335L). Conditions for the PCR were as follows ...
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bioRxiv - Cell Biology 2024Quote: ... and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1, NEB, E7335L). Conditions for the PCR were as follows ...
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bioRxiv - Bioengineering 2024Quote: ... along with a well containing 10 µl Quick-Load® Purple 1 kb DNA Ladder (New England Biolabs catalog no ...
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bioRxiv - Cell Biology 2024Quote: ... in a reaction solution containing 50 mM sodium phosphate (pH 7.5) and 1% NP-40 (B2704S, NE BioLabs) for 1 h at 37°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... Recovery PCR reactions were carried out with 1 µl of the selection product in Taq DNA polymerase (NEB) reactions with recovery primers (CSR_Rec_SHORT_F1/R1 ...
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bioRxiv - Genomics 2024Quote: ... 1 μl of 100 pmol dCTP and 0.5 μl of terminal transferase (20 units/μl) (New England Biolabs), and the mixture was incubated for 1 hour at 37°C followed by heating for 10 min at 70°C ...
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bioRxiv - Genomics 2024Quote: ... Index PCR was performed using the NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) (NEB #E7600S). The final library purification was carried out using 0.78x Ampure XP beads to remove the adaptor peak ...
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bioRxiv - Developmental Biology 2024Quote: ... 10 µl of DNAse-treated RNAwas mixed with 1 µl M-MuLV reverse transcriptase enzyme (New England Biolabs), 2 µl of 60 μM random primers ...
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bioRxiv - Cell Biology 2024Quote: ... The following primary antibodies used in this study were: anti-GFP from rabbit (1:5000, Torrey Pine Biolabs), anti-Myc 9E10 from mouse (1:2000 ...
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A gated hydrophobic funnel within BAX binds long-chain alkenals to potentiate pro-apoptotic functionbioRxiv - Cell Biology 2024Quote: ... Parental plasmids were digested by 1 μl DpnI enzyme (Cat. No. R0176, New England Biolabs, Ipswich, MA, USA) at 37°C for 15 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1, NEB, E7335L). Conditions for PCR used are as follows ...
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bioRxiv - Cell Biology 2024Quote: ... Germany). The sgRNAs (Suppl. Table 1) were generated by in vitro transcription with a T7 RNA polymerase (NEB) from the pSpCas9(BB)-2A-Puro (PX459 ...
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bioRxiv - Cancer Biology 2024Quote: ... 2.5 µl of indexed primers (NEBNext Multiplex Oligos for Illumina, Index primer set 1, NEB, cat. no. E7335), and the NEBNext High Fidelity PCR Master Mix (12.5 µl of 2x master mix ...
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bioRxiv - Cancer Biology 2024Quote: ... Illumina loop adaptors (2.5 µl, NEBNext Multiplex Oligos for Illumina, Index primer set 1, NEB, cat. no. E7335S) were ligated to the DNA fragments in 50 µl of the Quick ligation mix containing 25 µl of 2x Quick Ligase reaction buffer (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... and fragmented by sonication for 1 min and subjected to streptavidin bead-mediated precipitation (New England Biolabs, S1420S) of biotinylated fragments followed by purification and qPCR detection of the TERT promoter ...
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bioRxiv - Biophysics 2021Quote: ... in 500 μl T4 ligase buffer (50 mM Tris-HCl, 10 mM MgCl2, 1 mM ATP and 10 mM DTT, pH 7.5, NEB). Before adding the ligase ...
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bioRxiv - Cancer Biology 2021Quote: ... 70 mM Tris-Hydrochloride (Tris-HCl, pH 7.6 at 25°C) and 1 U/µl T4 PNK (New England BioLabs). Reaction mixtures were incubated at 37°C for 30 min ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 μg protein lysate was mixed with 1 pmol ssDNA substrate (IDT, Germany) and 0.75 U uracil-DNA glycosylase (UDG; New England Biolabs) in a total volume of 12 μL and incubated at 37 °C for 1 h ...
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bioRxiv - Molecular Biology 2020Quote: ... 40 μg/ml phenylmethylsulfonyl fluoride and protease inhibitor) and resuspended again in 1 ml MNase digestion buffer with 1,250 Units MNase (NEB Biolabs). Chromatin-MNase mix was incubated at 37 °C for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... 40 µl of the cleared lysates were incubated with 1 µl Lambda Protein Phosphatase (λ phosphatase) (New England Biolabs), 5 µl 10x buffer for Protein MetalloPhosphatases (New England Biolabs) ...
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bioRxiv - Cell Biology 2020Quote: ... coli CJ236 was picked into 1 ml of 2YT media (Roth, 6676.2) supplemented with M13KO7 helper phage (NEB, N0315) to a final concentration of 1 × 108 pfu/ml and ampicillin (final concentration 100 µg/ml ...
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bioRxiv - Genetics 2021Quote: ... 1μl XhoI digestion mix consisting of 5U XhoI restriction enzyme and 1 x NEB buffer 2.1 (New England Biolabs), and 10 ng genomic DNA for iciHHV-6B samples or 200 ng DNA for non-iciHHV-6B samples ...
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bioRxiv - Developmental Biology 2021Quote: ... and 10 μM of the dsAdR adaptor along with 1 μl (400 U) T4 DNA Ligase (New England Biolabs) were added in each ...
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bioRxiv - Genetics 2021Quote: ... EagI/KpnI-digested ORFs were ligated into the EagI/KpnI-digested pUASTattB vector backbone using a 6:1 insert:vector molar ratio and T4 DNA ligase (M0202S, NEB) in a thermocycler overnight (∼16 hr) ...
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bioRxiv - Developmental Biology 2020Quote: ... 75°C) with 0.5 μg mouse Cot-1 DNA supplemented with 10 mM vanadyl ribonucleoside complex (VRC) (NEB, S1402S) and 0.2 U μL−1 RNAseOUT (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: The rat HA-tagged PRG-1 ORF construct was generated by Gibson Assembly Cloning from NEB (Ipswich, MA, #E5510S) of the Plppr4 ORF clone (NM_001001508.2 ...
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bioRxiv - Molecular Biology 2020Quote: ... The un-crosslinked suspension was treated with 1 μg of RNase A at 37°C for 30 min and with 0.8 units of Proteinase K (NEB) at 45°C for 45 min ...
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bioRxiv - Molecular Biology 2020Quote: ... The plug was equilibrated for 1 h at room temperature in 100 μl 1x Quick Ligation buffer (NEB B6058S) containing 2.7 μl END-seq adaptor 1 ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mg of cell lysate was cleared with protein A magnetic beads to remove non-specific interactions (S1425S, NEB) and incubated overnight with anti-ZAP antibody (Proteintech 16820-1-AP ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and resuspended in 50 µL of 1 mg/mL RNAse A solution (New England Biolabs, Ipswich, Massachusetts, United States) and statically incubated at 37 °C for 3 h ...
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bioRxiv - Genomics 2020Quote: ... were combined with RT primer mix [1 μl 250 ng/μl randomhexRT primer and 0.5 μl 10 mM dNTPs (NEB)] ...
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bioRxiv - Genomics 2020Quote: ... before being resuspended in 1X ligase (1X T4 ligase buffer, 1:100 dilution of T4 DNA ligase NEB #M0202S) and incubated at room temperature for one hour ...
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bioRxiv - Molecular Biology 2021Quote: ... The annealed oligos were diluted 1:50 and then ligated into 50 ng NotI+BamHI-digested pBRα or pACλCI using T4 ligase (NEB), generating pS197 and pS198 ...
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bioRxiv - Microbiology 2020Quote: ... were carried out using specific primers (Supplemental Table 1) and the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). After sequence verification ...
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bioRxiv - Developmental Biology 2020Quote: ... oligonucleotides for the HAS-1 or HAS-2 sgRNA guide sequences were phosphorylated using T4 PNK (NEB, Ipswich, MA) for 30 min at 37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... PCR was performed on 0.5 – 1 ng of genomic DNA with the LongAmp Taq DNA Polymerase (New England Biolabs) using standard PCR reaction mix and long extension times (9 minutes at 65°C).
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bioRxiv - Genomics 2020Quote: Cas9 protein and transcribed sgRNA were incubated for 10 min at room temperature in reaction buffer containing 1× NEB buffer 3.1 (NEB Biolabs) supplemented with 1 mM DTT ...
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bioRxiv - Synthetic Biology 2020Quote: ... and subsequently mixed at a 6:1 ratio with the digested backbone in reactions containing T4 DNA ligase (NEB).
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bioRxiv - Biochemistry 2020Quote: ... 1 μg ml−1 of template DNA was mixed with 1000 U ml−1 of T7 polymerase and 1.5 mM of each rNTP (New England Biolabs) in 40 mM Tris-HCl ...
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bioRxiv - Genetics 2020Quote: Total RNA was extracted using the standard hot acid phenol method and treated with DNase 1 (New England Biolabs). qRT-PCR was performed using amfiRivert cDNA synthesis Platinum Master Mix from Gendepot ...
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bioRxiv - Genomics 2021Quote: ... 1% of the cDNA was used for each qPCR reaction performed with the Luna Universal qPCR Mastermix (NEB M3003) and the PCR primers listed (Supplementary Table1) ...