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Citations for New England Biolabs :
2701 - 2750 of 10000+ citations for Thyroxine T4 ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... as well as 1.5 U of T4 DNA polymerase and NEBuffer 2.1 (New England Biolabs). The reaction was incubated for 4 minutes at 22 °C and then stopped by addition of 10 mM dCTP ...
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bioRxiv - Biochemistry 2021Quote: ... Annealed bubble templates and digested 601-R fragments were ligated using T4 DNA ligase (NEB) and ligated products were further purified through Model 491 Prep Cell to remove free bubbles and 601R fragments.
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bioRxiv - Molecular Biology 2020Quote: ... the ends of the NcoI/BsrGI-digested plasmid were filled with T4 DNA polymerase (NEB) according to the manufacturer’s protocol and blunt ends were re-ligated ...
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bioRxiv - Biophysics 2021Quote: ... 1 mM DTT at pH 7.9) containing 30 units/ml of T4 DNA polymerase (NEB), 100μg/ml single stranded DNA binding protein (gp32 ...
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bioRxiv - Genomics 2021Quote: ... to 3’-P presenting in the RNA that was dephosphorylated using T4 PNK (NEB, #M0201S). Linker and RNA ligation was performed by ligating pre-adenylated linker to the 3’-OH of RNA using RNA ligase2 ...
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bioRxiv - Microbiology 2021Quote: ... 10 μl NEBNext Quick T4 DNA Ligase NEBNext Quick Ligation Module (New England BioLabs, 6056) and 5 μl Adapter Mix (AMX) ...
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bioRxiv - Biochemistry 2020Quote: ... was ligated using T4 DNA ligase and the ligation buffer (obtained from New England Biolabs). Each reaction mixture containing 300ng nicked plasmid and 200U ligase was incubated for 1 hr at 37° C before inactivating the enzyme by SDS addition (0.2% final ...
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bioRxiv - Biochemistry 2021Quote: ... When needed purified RNA was treated with T4 polynucleotide kinase (PNK) (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Biochemistry 2021Quote: ... When needed purified RNA was treated with T4 polynucleotide kinase (PNK) (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Genomics 2021Quote: ... consisting of 150 μL 10X NEB T4 DNA ligase buffer with 10 mM ATP (NEB), 125 μL 10% Triton X-100 ...
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bioRxiv - Biochemistry 2021Quote: ... oligonucleotide annealing was carried out in a thermocycler in T4 polynucleotide kinase reaction buffer (NEB) / 50 mM potassium acetate by heating oligonucleotide mixtures to 95 °C for 5 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... digested genomic DNA by O/N incubation at 16°C with T4 DNA ligase (NEB). Subsequently ...
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bioRxiv - Synthetic Biology 2021Quote: ... Using golden gate cloning (BsaI-HF®v2 NEB #R3733L, T4 DNA ligase NEB #M0202T), in this plasmid ...
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bioRxiv - Synthetic Biology 2021Quote: ... Using golden gate cloning (BsaI-HF®v2 NEB #R3733L, T4 DNA ligase NEB #M0202T), in this plasmid ...
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bioRxiv - Developmental Biology 2021Quote: ... and the fragments were ligated over night at 4°C with T4 DNA Ligase (NEB) and heat shock transformed into DH5α competent E ...
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bioRxiv - Cell Biology 2020Quote: ... Each pair of oligos was annealed and phosphorylated with T4 PNK (NEB, Cat No. M0201S) in the presence of 10× T4 DNA ligase buffer in a thermocycler with the following parameters ...
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bioRxiv - Genomics 2021Quote: ... The 3′ adapter (sequence: AGATCG-GAAGAGCACACGTCTGAACTC) was ligated using T4 RNA Ligase 1 (NEB, M0204L) and purified nascent RNA using streptavidin beads (NEB ...
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bioRxiv - Microbiology 2019Quote: ... and the DNA fragments were then circularized by using T4 DNA ligase (New England BioLabs). Primers ermF and ermR (Table 2 ...
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bioRxiv - Molecular Biology 2021Quote: ... The purified products were ligated using T4 DNA ligase (New England Biolabs, Ipswich, MA, USA). The ligated product was transformed into Escherichia coli (E ...
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bioRxiv - Molecular Biology 2020Quote: ... The ends of the fragmented RNA were repaired by treatment with T4 Polynucleotide Kinase (NEB) and Calf intestinal alkaline phosphatase (NEB ...
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bioRxiv - Molecular Biology 2020Quote: 3’ linker ligation (1x PNK buffer, 800 u T4 RNA ligase 2 truncated KQ (NEB), 80 u RNaseOUT ...
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bioRxiv - Cancer Biology 2020Quote: ... extra Biotin-14-dATP was first removed using 15 units of T4 DNA polymerase (NEB) and incubating at 20°C for 4 hours ...
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bioRxiv - Bioengineering 2022Quote: ... 400 U of T4 DNA ligase (1 µL of 400 U/µL) (NEB; catalog # M0202L), and 1X T4 DNA ligase buffer (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... gRNA was phosphorylated by a T4 polynucleotide kinase according to product instructions (New England Biolabs). For the amplification of phosphorylated sgRNA it was incubated with 1 μl of forward and reverse primers (100 μM ...
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bioRxiv - Immunology 2022Quote: ... and incubated with a ligation reaction mixture (10 unit/μL T4 DNA ligase (NEB, M0202M), 1x T4 DNA ligase buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... chilled on ice and then combined with 30 U T4 RNA Ligase 1 (NEB M0437M), 40 U Murine RNase Inhibitor (NEB) ...
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bioRxiv - Systems Biology 2023Quote: ... The digested DNA library and the digested vector were ligated with T4 DNA ligase (NEB). The ligation reaction was precipitated with isopropanol and transformed into MegaX DH10B T1R Electrocompetent Cells (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2022Quote: ... and ligated over night at 16°C using T4 DNA ligase (New England Biolabs, UK). The ligation mix was transformed into E ...
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bioRxiv - Genomics 2022Quote: ... and DNA was repaired for 30 minutes with a mixture of T4 DNA ligase (NEB), T4 DNA polymerase I (NEB) ...
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bioRxiv - Synthetic Biology 2022Quote: ... were digested with Nco I and Fse I and ligated using T4 DNA ligase (NEB) to generate AGG1245.
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bioRxiv - Synthetic Biology 2024Quote: ... All molecular biology enzymes (BsaI-HFv2, T4 DNA ligase, Q5 polymerase) were supplied by NEB. Synthetic DNA fragments and oligonucleotides were supplied by either IDT or Twist Bioscience ...
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bioRxiv - Synthetic Biology 2024Quote: Cloning was performed using restriction enzyme digestion and ligation with T4 ligase (New England Biolabs) or Gibson assembly (New England Biolabs) ...
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bioRxiv - Molecular Biology 2024Quote: ... and fragmentation (25 min at 94 °C in 2X T4 PNK reaction buffer from NEB –140 mM Tris-HCl pH 7.6 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we added 1 uL of T4 DNA ligase buffer (New England Biolabs, USA, product #B0202S). Using a thermal cycler (C1000 Touch Thermal Cycler ...
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bioRxiv - Molecular Biology 2024Quote: ... and ligated into SfiI digested backbone from pCH117 using T4 DNA ligase (New England Biolabs). All promoter variants were verified by Sanger sequencing (Elim Biopharmaceutics Inc).
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bioRxiv - Molecular Biology 2024Quote: ... Ligations of the dsDNA oligos to the digested pJMP1339 were performed with T4 ligase (NEB) in a 10 µl reaction volume with 50 ng of digested pJMP1339 and 2 µl of diluted oligos ...
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bioRxiv - Molecular Biology 2024Quote: ... Annealed products were ligated to the cleaved px330 plasmid using T4 DNA ligase (NEB, M0202T) and specific buffer (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified fragments were dephosphorylated at their 3′ ends with T4 polynucleotide kinase (New England Biolabs) at 37 °C for 1 h and ligated to a 3′ adaptor sequence using T4 RNA ligase 2 (truncated K227Q ...
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bioRxiv - Biochemistry 2023Quote: ... Labelled handles were ligated with the central part with T4 DNA Ligase (New England Biolabs) for 15 h at 16°C followed by 1 h at 37°C before heat inactivation ...
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bioRxiv - Immunology 2023Quote: ... RNA was then ligated to the MARSseq ligation adaptor with T4 RNA Ligase I (NEB) at 22°C for 2h ...
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bioRxiv - Genetics 2024Quote: ... The ds-oligos were then ligated into BbsI-digested pCFD3 vector with T4 ligase (NEB). Following cloning ...
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bioRxiv - Cancer Biology 2024Quote: ... Hi-C libraries were treated with T4 DNA Polymerase (New England Biolabs, catalog No. M0203) to remove biotin from unligated ends ...
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bioRxiv - Cancer Biology 2024Quote: ... The digested plasmid and phosphorylated oligo cassettes were ligated with T4 DNA ligase (NEB #M0202L) at room temperature for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... For the ligation reaction 8 µL of the digest was treated with T4 Ligase (NEB) for 16 hours at 16 °C ...
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bioRxiv - Plant Biology 2023Quote: ... DNA adaptator was ligated overnight with 2,000 u of T4 DNA ligase (New England Biolabs). Two PCRs were carried out by using nested primers designed on the adaptator and on the paromomycin resistance gene using the Advantage® GC genomic LA polymerase mix (Clontech) ...
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bioRxiv - Molecular Biology 2023Quote: ... the ligation reaction was performed by introducing 10 units T4 DNA ligase enzyme (NEB, USA) and incubating at 20 °C for 1 hour to seal the 5’ and 3’ ends of the ssPCRP ...
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bioRxiv - Bioengineering 2023Quote: ... T4 Ligase and low molecular-weight DNA ladder were obtained from New England Biolabs (NEB). Methyltetrazine DBCO ...
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bioRxiv - Biophysics 2023Quote: ... and ligated to the hairpin with short handles with T4 DNA ligase (New English Biolabs) to finish the 537-bp hairpin preparation.
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bioRxiv - Synthetic Biology 2023Quote: All ligases (T3, T4, E.coli, T7, Taq) used during this work were obtained from NEB. Ligations were performed as indicated by the supplier ...
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bioRxiv - Genetics 2023Quote: ... Synthetic oligonucleotides were labeled with [γ 32P]-ATP and T4 polynucleotide kinase (New England Biolabs) at their 5’ end ...