Labshake search
Citations for New England Biolabs :
2501 - 2550 of 3709 citations for 1 1' Diethyl 4 4' bipyridinium dibromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... Receptors were surface labeled by addition of SNAP-Surface Alexa Fluor 546 (1:1000, New England Biolabs) to DMEM F12 without serum and phenol red (21041-025 ...
-
bioRxiv - Neuroscience 2023Quote: ... 72°C – 1 min]) with NEBNext® High-Fidelity 2X PCR Master Mix (New England Biolabs, #M0541S) and barcoded Nextera primers (1.25 μM each ...
-
bioRxiv - Synthetic Biology 2023Quote: ... 1 μg RCA product was restricted by employing 10 U nicking endonuclease Nb.BbvCI (New England Biolabs, R0631L) in a 50 μL reaction volume with 1x rCutsmart buffer for 1 h at 37 °C ...
-
bioRxiv - Microbiology 2023Quote: ... 20 uL of sheared DNA was then digested with 1 U of DpnI in cutsmart buffer (NEB) for 3 hours at 37°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... The samples were multiplexed with NEBNext Multiplex Oligos for Illumina (Index Primers Set 1 and 2) (NEB). Size selection steps were performed with Ampure XP beads (Beckman Coulter ...
-
bioRxiv - Microbiology 2023Quote: ... cDNA was reverse transcribed from 1 µg of total RNA using LunaScript RT Supermix kit (NEB #E3010) according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... The insert was ligated to the vector in a 3:1 ratio (insert:vector) using T4 ligase (NEB). This introduced C-terminal 6xHis tag to Syn0852 ...
-
bioRxiv - Cell Biology 2024Quote: ... 1 µg of total RNA sample (with RIN ≥ 7) was enriched for mRNA (New England Biolabs, #E7490) for each condition ...
-
bioRxiv - Cancer Biology 2024Quote: ... and libraries prepared using NEBNext Multiplex Oligos for Illumina Index Primer Set 1 (New England Biolabs, E7335S) and NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs ...
-
bioRxiv - Microbiology 2024Quote: ... The phages were then treated with 100 μg·ml-1 of Proteinase K (New England Biolabs, Whitby, Canada) with SDS to a final concentration of 2% (v·v-1 ...
-
Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... followed by a secondary Alexa Fluor 555 conjugated anti-mouse IgG antibody (NEB #4413S; diluted 1:1000). For flow cytometry ...
-
bioRxiv - Genetics 2024Quote: ... PCR samples were combined with 1 μL of 6X gel loading dye (New England Biolabs, Cat. # B7024S) per 5 μL of sample and loaded each the remaining wells ...
-
The triad interaction of ULK1, ATG13, and FIP200 is required for ULK complex formation and autophagybioRxiv - Cell Biology 2024Quote: ... MBP-FIP200 (1–634) was purified by affinity chromatography using Amylose Resin High Flow (New England Biolabs). Next ...
-
bioRxiv - Microbiology 2024Quote: ... A polyC tail was added to 1 µg of end repaired DNA using Terminal Deoxynucleotidyl Transferase (NEB) with a mixture of 95% dCTP and 5% ddCTP as a substrate ...
-
bioRxiv - Microbiology 2024Quote: ... using a specific primer pair (Supplementary table 1) and Q5 High-Fidelity DNA Polymerase (New England Biolabs). The PCR product was purified using a MinElute Reaction Cleanup kit (Qiagen) ...
-
bioRxiv - Microbiology 2024Quote: ... The 1 kb regions were assembled into the suicide plasmid pK18mobsacB72 using Gibson Assembly73 (New England Biolabs) and the resulting pK18-Δbd2269 was transformed into E ...
-
bioRxiv - Plant Biology 2024Quote: ... the MBP tag was enzymatically cleaved using 10 μg ml-1 Xa factor protease (NEB, Cat#P8010) at 23°C overnight ...
-
bioRxiv - Molecular Biology 2024Quote: ... 30 µL of RNA was premixed with 1 µL of 200 ng/µL Random Primer 9 (NEB) and 2 µL 100 mM dNTPs (NEB ...
-
bioRxiv - Biophysics 2024Quote: ... The resulting annealed structure was then combined with 1 µL of DraIII-HF (NEB; 100,000 units/mL) and 1 µL of BaeGI-HF (NEB ...
-
bioRxiv - Biochemistry 2024Quote: ... 1 mM CaCl2 and the indicated volumes of 2 units bovine pancreatic DNase I (New England Biolabs) were added for the indicated incubation times at 37 °C ...
-
bioRxiv - Neuroscience 2024Quote: ... tube 2: 20 µL VLP suspension + 6 µL water + 1 µL Proteinase K (200 µg/mL, NEB), and tube 3 ...
-
bioRxiv - Plant Biology 2024Quote: ... After washing twice with nuclei isolation buffer and one round with 1× NEB Dpn II buffer (NEB), nuclei pellet was resuspended with 250 µl 0.5% SDS and split into five tubes ...
-
bioRxiv - Neuroscience 2024Quote: ... 200 ng of the amplified gDNA region product and 1 μM EnGen® Sau Cas9 (#M0654, NEB), according to the manufacturer ...
-
bioRxiv - Plant Biology 2024Quote: ... Reverse transcription was carried out on 1 μg total RNA after DNase I-digestion (New England Biolabs) using the SuperScript III kit (Fisher Scientific ...
-
bioRxiv - Synthetic Biology 2024Quote: ... was mixed with 0.5 µL of ATP (100 mM) and 1 µL of T4 PNK (NEB, UK) with a 33.5 µL normalized DNA solution (approx ...
-
bioRxiv - Genomics 2024Quote: ... 1 μg of amplicons from each target was treated with M.SssI CpG methyltransferase (New England Biolabs, M0226S) per the vendors recommendations ...
-
bioRxiv - Biochemistry 2024Quote: RT-PCRs were performed on 1:100 diluted RT samples using hot start Taq DNA polymerase (NEB) supplemented with extreme thermostable ssDNA-binding protein (ET SSB ...
-
bioRxiv - Biochemistry 2024Quote: ... 5 µl of each sample was mixed with 1 µl of 6X Purple Gel Loading Dye (NEB) and electrophoresed at 110 V for 30 minutes ...
-
bioRxiv - Biochemistry 2024Quote: ... TdT (20 U) and α-32P-dATP (3.3 pmol) were prepared in 1 x TdT buffer (NEB) and incubated at 37 °C for 1 h ...
-
bioRxiv - Systems Biology 2024Quote: ... each with 40μL of FACS buffer with 1:1000 diluted Murine RNase Inhibitor (New England Biolabs, M0314L). After collection ...
-
bioRxiv - Genomics 2024Quote: ... and ligated with methylated adapters for Illumina sequencing at a dilution of 1:10 (New England BioLabs). The adapter ligated DNA was subjected to bisulfite conversion with EpiTect fast bisulfite conversion kit (Qiagen ...
-
bioRxiv - Bioengineering 2024Quote: ... ddPCR reactions were incubated at room temp for 1 hour with 1E5 units of SalI (NEB R3138L) to facilitate digestion of linear cAAV insertion products prior to droplet generation ...
-
bioRxiv - Genomics 2024Quote: ... The RCA-MERFISH probes were ligated with 10% v/v (∼1 uM) SplintR ligase (New England Biolabs), 1× SplintR ligase buffer ...
-
bioRxiv - Cell Biology 2024Quote: ... 0.8 M guanidine HCl) containing 8 units mL−1 of Proteinase K (P8107S, New England Biolabs Inc.). Gels were digested at room temperature overnight ...
-
bioRxiv - Cell Biology 2024Quote: ... Embryos were injected with 1 mg of gRNA and 5 μ M of Cas9 protein (NEB, 10128356) at 1-cell stage ...
-
bioRxiv - Cancer Biology 2024Quote: ... for 2 h followed by 1 h incubation with 10 units of CIP Alkaline phosphatase (#M0290, NEB). The digested DNA samples were purified and added to a BrdU conjugate preadsorbed microplate prepared according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2024Quote: ... Denatured complexes were run on a 1% agarose gel containing 1X Sybr Safe stain (New England Biolabs).
-
bioRxiv - Cancer Biology 2024Quote: ... The guides were amplified by PCR and cloned into pKLO.1 by ligation using the Esp3I (NEB) restriction sites39,40 ...
-
bioRxiv - Genomics 2021Quote: ... the purified products are treated with Klenow fragment (3’ → 5’ exo-) (Cat. No. M0212L; NEB; use 1 uL) and Taq DNA polymerase (Cat ...
-
bioRxiv - Genetics 2021Quote: ... 1 μg of RNA was reverse transcribed in cDNA using random hexamers and M-MuLV reverse transcriptase (NEB). qPCRs were assembled with Absolute QPCR ROX Mix (Thermo Scientific ...
-
bioRxiv - Molecular Biology 2021Quote: ... Backbone and annealed guide template were ligated for 1 h at room temperature using T4 DNA Ligase (NEB), 100 ng backbone ...
-
bioRxiv - Evolutionary Biology 2021Quote: Hybridization with the reverse transcription primers was performed by adding 1 uL of SR RT Primer (NEB E7333A) to the above reaction and incubating at 75 C for 5 minutes ...
-
bioRxiv - Evolutionary Biology 2021Quote: Both HLS-C and HLS-E protein at 1 mg/mL were incubated with human Furin (EC 3.4.21.75, P09958, obtained from NEB P8077) in digestion buffer (10 mM HEPES (pH 7.5) ...
-
bioRxiv - Developmental Biology 2020Quote: ... 2014) by incubating 0.25 μl of plasmid with 1 μl of recombinant Cre recombinase (New England Biolabs M0298S) according to manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2022Quote: One μL of amplicons from colony PCR is mixed with 1 μL of Gel Loading Dye (6x; NEB) and 4 μL of nuclease-free water in each well on the 1.2% agarose gel (Sigma ...
-
bioRxiv - Genomics 2020Quote: ... except that all ligations were supplemented with 1:40 RNAse inhibitor (ThermoFisher Ribolock or NEB Murine RNase Inhibitor) to prevent RNA degradation ...
-
bioRxiv - Molecular Biology 2020Quote: ... 200 ng of PCR product of RFLP_region 1 and RFLP_region 2 were digested with PstI (New England Biolabs) and VspI (Thermo Fisher Scientific ...
-
bioRxiv - Genetics 2021Quote: ... we used NEBNext® Multiplex Small RNA Library Prep Set for Illumina®(Set 1) (catalog#E7300S,NEB) (as described13) ...
-
bioRxiv - Biochemistry 2020Quote: ... Cas9 RNPs were formed by incubating 1 pmol of sgRNA with 0.5 pmol of Cas9 protein in 1X NEBuffer™ 3.1 or 2.1 (NEB) at room temperature for 10 min ...
-
bioRxiv - Biochemistry 2020Quote: ... by incubating the cells with 2 μM ATTO594-Coenzyme A and 1 μM phosphopantetheine transferase (New England Biolabs) in Ham’s F12 medium without FBS at ~25°C for 20 min ...