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Citations for New England Biolabs :
2501 - 2550 of 10000+ citations for PCR Genotyping kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: PCR amplifications were performed using Phusion High-Fidelity DNA polymerase (New England Biolabs) and oligonucleotides for cloning were obtained from Integrated DNA Technologies ...
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bioRxiv - Genomics 2023Quote: ... PCR was performed with Q5U HotStart High-Fidelity polymerase (New England Biolabs, M0515) as usual except a custom dNTP mix was used that contained dATP ...
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bioRxiv - Genomics 2023Quote: ... using Q5 High-Fidelity PCR master mix with HF buffer (NEB Ipswich, MA) and PCR primers containing the T7 promoter sequence (Forward ...
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bioRxiv - Cancer Biology 2023Quote: The coding sequence of bwCIRBP was amplified by PCR using Phusion polymerase (NEB), digested with EcoRI and NotI ...
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bioRxiv - Microbiology 2023Quote: ... The resulting PCR fragment was digested with EcoRI (New England Biolabs, Cat# R3101S) and BamHI (New England Biolabs ...
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bioRxiv - Synthetic Biology 2023Quote: ... PCR amplification was performed using Q5® High-Fidelity DNA Polymerase (NEB #M0491L). The original vector was digested with the restriction enzyme DpnI (NEB #R0176S ...
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bioRxiv - Microbiology 2023Quote: PCR was performed using Q5 High-Fidelity 2X Master Mix (New England Biolabs) as per manufacturer’s instructions with the following primers ...
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bioRxiv - Synthetic Biology 2023Quote: ... Phusion® High-Fidelity PCR Master Mix with HF Buffer (New England BioLabs) was used to screen C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we PCR-amplified the plasmids’ inserts using Q5 polymerase (NEB, USA product #M0491) (see “Molecular Cloning” for protocol) ...
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bioRxiv - Genomics 2024Quote: ... PCR products were then digested at 37°C with DpnI (New England Biolabs) for 2 hours to remove residual starting plasmid ...
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bioRxiv - Genetics 2024Quote: ... The PCR-amplified oligo pool was then digested with BsaI-HFv2 (NEB #R3733S), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... The PCR reaction mix was created with NEB Phusion GC Buffer (NEB, B0519S), Phusion DNA Polymerase (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... Mutagenic PCR products were then digested with 10 U DpnI (New England Biolabs) at 37°C for 30 min to eliminate template DNA ...
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bioRxiv - Cell Biology 2024Quote: All PCR amplification was completed using Q5 High-Fidelity DNA Polymerase (NEB #M0491S) for all PCR amplifications including site-directed mutagenesis.
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bioRxiv - Genomics 2024Quote: ... PCR amplification with was Q5® High-Fidelity DNA Polymerase (New England Biolabs) with CFTR Exon 8 forward primer (5’-TTTCTGTTGGTGCTGATATTGCCTCAGGGTTCTTTGTGGTGT-3’ ...
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bioRxiv - Cell Biology 2024Quote: ... The 3’ homology arm was amplified by PCR (Q5 HiFi polymerase®, NEB), from HCT116 cell genomic DNA using the primers NCAPH2 3’ homology arm forward and reverse ...
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bioRxiv - Cell Biology 2024Quote: ... which were generated via PCR using the Q5 DNA Polymerase (New England BioLabs), purified with HighPrep PCR Clean-up beads (MagBio) ...
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Emerging cooperativity between Oct4 and Sox2 governs the pluripotency network in mouse early embryosbioRxiv - Developmental Biology 2024Quote: ... 10.0 μl NEBNext High-Fidelity 2×PCR Master Mix (New England Biolabs, M0541S), 0.25 μl 100 μM PCR Index 1 ...
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bioRxiv - Bioengineering 2024Quote: ... PCR was done with the Q5® High-Fidelity 2X Master Mix (NEB). After PCR1 in the nested PCR strategy ...
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bioRxiv - Genomics 2024Quote: ... and PCR amplified for 2 cycles using the NEBNext master mix (NEB M0544L) with Illumina Nextera primers and conditions ...
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bioRxiv - Bioengineering 2024Quote: ... Genotypes were then confirmed by colony PCR with OneTaq (New England Biolabs, UK) and selected for antibiotic sensitivity ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR fragments were amplified using Thermo Scientific Phusion High-Fidelity DNA Polymerase (NEB) and ligated into entry vectors through BsaI-restriction cloning ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR fragments and the linearized pITR2 vector were digested with SfiI (NEB, R0123S), purified ...
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bioRxiv - Immunology 2024Quote: ... promoting amplification of this region via Q5 polymerase-based PCR (New England Biolabs). PCR products were then identified by agarose gel method and purified via Zymoclean Gel DNA Recovery Kit (Zymo Research) ...
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bioRxiv - Systems Biology 2024Quote: ... 28 cycles) using the Phusion® High-Fidelity PCR Master Mix (NEB, M0531L). Samples were verified using gel electrophoresis ...
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bioRxiv - Systems Biology 2024Quote: ... Quantitative PCR was performed with Luna Universal qPCR Master Mix (New England BioLabs) using an Applied Biosystems ViiA 7 Real-Time PCR system ...
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bioRxiv - Synthetic Biology 2024Quote: ... We used Phusion High-Fidelity PCR MasterMix with HF Buffer (NEB, Catalog #M0531L) for the second PCR and used the following PCR conditions ...
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bioRxiv - Molecular Biology 2024Quote: ... purified PCR products were assembled using NEBuilder HiFi DNA Assembly Master Mix (NEB). Assembled gRNA/Cas9 and donor plasmids were transformed into DH5α bacteria and final purified plasmids were checked by sanger sequencing (Genewiz).
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bioRxiv - Developmental Biology 2024Quote: ... qRT-PCR was performed using the Luna Universal qPCR Master Mix (NEB, M3003L) in a BioRad machine ...
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bioRxiv - Biophysics 2019Quote: ... the 5’ ends of the PCR products were phosphorylated by T4 polynucleotide kinase (NEB), the products were purified and the free ends of the plasmid re-ligated by T4 DNA ligase (NEB) ...
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bioRxiv - Cancer Biology 2021Quote: ... The PCR products were end-repaired with T4 DNA polymerase (NEB; Cat. No. B02025), DNA Polymerase I ...
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bioRxiv - Microbiology 2020Quote: ... Three PCR fragments were produced using the Q5 DNA polymerase system (New England Biolabs) according to the manufacturer’s instructions (54) ...
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bioRxiv - Cell Biology 2020Quote: All PCRs for cloning procedures were performed using NEB Phusion polymerase (New England Biolabs). Primers used for cloning ...
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bioRxiv - Genetics 2021Quote: ... PCR was used to amplify DNA fragments and Gibson assembly cloning (New England Biolabs) employed to assemble fragments together ...
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bioRxiv - Genetics 2021Quote: ... These Promoters were amplified by PCR and individually inserted by Gibson assembly (#E2611S; NEB) into the Downstream assay vector ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR was performed using the OneTaq-Quick-Load 2X Master Mix (New England BioLabs) with Aedes whole-body cDNA as a template ...
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bioRxiv - Immunology 2021Quote: ... CD4 was amplified by PCR using Q5 DNA Hot Start Polymerase (New England BioLabs) in 0.2µM dNTP ...
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bioRxiv - Developmental Biology 2021Quote: The PCR product was digested with the restriction enzyme BsaI (New England Biolabs, R0535L) to yield a 168 bp fragment for the wild-type allele and a 120 bp and a 48 bp fragments for the mutant allele ...
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bioRxiv - Evolutionary Biology 2021Quote: ... This 20 μL 10-cycle PCR reaction was performed using Q5 polymerase (NEB M0491L) following the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2020Quote: ... The gel-purified PCR fragments were incubated with HiFi DNA assembly enzyme mix (NEB) and transformed into E ...
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bioRxiv - Cell Biology 2022Quote: ... PCR product was then assessed using a ClaI restriction enzyme digest (New England BioLabs) per manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR mutagenesis for generating new BASIC parts were carried out using Phusion Polymerase (NEB) and phosphorylated oligonucleotides (IDT) ...
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bioRxiv - Molecular Biology 2021Quote: RNPS1 point and deletion mutants were PCR amplified using Q5 polymerase (New England Biolabs) and inserted into PB-CuO-MCS-BGH-EF1-CymR-Puro (modified from System Biosciences) ...
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bioRxiv - Molecular Biology 2020Quote: For cloning purposes all PCRs were done using Q5 high-fidelity DNA polymerase (NEB) and Gibson Assembly was performed using NEBuilder HiFi DNA assembly master mix (NEB ...
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bioRxiv - Molecular Biology 2020Quote: PCR was performed using Q5 High-Fidelity DNA Polymerase (New England Biolabs, Cat.# M0491L). PCR fragments were assembled using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Molecular Biology 2020Quote: ... and were PCR-amplified using Q5 Hot Start High-Fidelity DNA Polymerase (NEB, M0493) with 18 cycles.
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bioRxiv - Molecular Biology 2021Quote: ... PCR was done using Taq Polymerase according to the manufacturer’s instructions (New England Biolabs).
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bioRxiv - Developmental Biology 2022Quote: ... The complete insert region was PCR amplified using Q5 Hi Fidelity DNA polymerase (NEB) and the amplicon sequenced at high coverage using Illumina MiSeq in the Complete Amplicon Next-Generation Sequencing service from the MGH CCIB DNA Core (Fig ...
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bioRxiv - Genomics 2019Quote: ... The linearized fragments were PCR amplified with LongAmp Taq DNA Polymerase (New England Biolabs) and purified using 1x AmpureXP beads ...
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bioRxiv - Immunology 2021Quote: ... gBlocks were amplified by polymerase chain reaction (PCR) using Q5 polymerase (New England BioLabs) following the manufacturer’s protocol ...