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Citations for New England Biolabs :
201 - 243 of 243 citations for Fluorescein alkynylamino atp since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Circularized DNA templates were prepared by incubating 300 nM of template oligo with 200 nM padlock probe in a 50 μl ligation reaction containing 1mM ATP and 400U of T4 DNA ligase (New England Biolabs) in a buffer comprising 33 mM Tris.acetate (pH 7.9) ...
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bioRxiv - Biochemistry 2023Quote: Both native and modified oligonucleotides were 5’-32P labeled by [γ-32P]-ATP using T4 polynucleotide kinase (New England Biolabs) following manufactures recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... the beads were incubated in the 30 μl T4 PNK reaction mix (0.3 μl of 0.1 μCi/μl γ-32P ATP, 3U of T4 PNK enzyme (NEB M0201L) in 1X PNK reaction buffer containing 1mM DTT ...
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bioRxiv - Biochemistry 2024Quote: ... When relevant cmpRNA (500 pmol) was 5’-end phosphate-32 “hot” labeled using fresh gamma phosphate-32 labeled ATP (50 nmol) and PNK (NEB) following the producer’s recommendations ...
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bioRxiv - Genetics 2024Quote: ... and 5’end-labelling with 0.5 μl [γ-32P] ATP (6000 Ci/mmol, 25 μM, Perkin-Elmer) and 10 U of T4 PNK (NEB)was performed in T4 PNK buffer for 2 hours at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: Telomeric duplex DNA 5′-GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGCCCCTC-3′ and antisense (5′-GAGGGGCCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCC-3′ was end-labeled with [γ-32P]ATP (Amersham Biosciences) and T4-polynucleotide kinase (New England BioLabs) and purified from free nucleotides through G25 spin columns (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: ... The nuclei pellet was resuspended in the Ligation Buffer containing 120 μl 10X NEB T4 DNA Ligase Buffer supplemented with 10 mM ATP (NEB, B0202), 60 μl 20% Triton X-100 (1% final) ...
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bioRxiv - Molecular Biology 2021Quote: ... then incubated for 2 h at 37°C in 100 μl 1x TdT buffer containing 4 μl 10 mM ATP and 1 μl Terminal Transferase (NEB M0315L). Plugs were rinsed with 1 ml tris buffer (10 mM Tris HCl pH 8.0) ...
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bioRxiv - Cell Biology 2021Quote: ... and 200 ng of the purified amplicon were end-labeled using 10 µCi of γ-32P ATP (Amersham) and T4 polynucleotide kinase (NEB). The labeling reaction was then desalted on a G-25 spin column and further precipitated with 70% ethanol ...
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bioRxiv - Molecular Biology 2022Quote: ... corresponding to both strands of the 35S rRNA promoter from positions −180 to −110 and −110 to −40 were end-labelled using [γ-32P]ATP and T4 polynucleotide kinase (New England Biolabs) and purified on a 10% acryl/bisacrylamide ...
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bioRxiv - Biochemistry 2022Quote: ... the used RNA was radioactively labelled on the 5’-end with [γ-32P]ATP (10 mCi/ml, Hartmann Analytic) and T4 PNK (NEB), following purification via PAGE ...
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bioRxiv - Microbiology 2020Quote: ... Purified PCR products or annealed oligonucleotides were prepared as described above and end-labeled with 10 μCi [γ-32P]ATP (Perkin-Elmer) and 10 U T4 polynucleotide kinase according to established protocols (New England BioLabs). Binding reactions included 1 μl (~30 ng ...
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bioRxiv - Molecular Biology 2020Quote: ... then incubated for 2 h at 37°C in 100 μl 1x TdT buffer containing 4 μl 10 mM ATP and 2 μl Terminal Transferase (NEB M0315L). Plugs were rinsed with 1 ml tris buffer (10 mM Tris HCl pH 8.0) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Filled-in chromatin was then ligated by adding 970 μl of Ligation Master Mix (150 μl of 10x NEB T4 DNA ligase buffer with ATP (NEB, B0202), 125 μl of 10% Triton X-100 ...
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bioRxiv - Genetics 2022Quote: ... Specific sRNAs were detected by hybridization with DNA oligonucleotides labeled at their 5’ termini with [γ-32P]ATP and T4 Polynucleotide Kinase (New England Biolabs) as previously described (Ibrahim et al. ...
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bioRxiv - Biochemistry 2021Quote: ... at 37 °C for 1 hour or first with 5 units T4 Polynucleotide Kinase with 25 mM ATP in 1 X T4 Kinase buffer (T4PNK, NEB, M0236S) at 37 °C for 30 min and then with XRN-1 ...
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bioRxiv - Cell Biology 2020Quote: A synthetic miR-409-5p oligo was 5’ end-labelled with γ-P32 ATP using T4 polynucleotide kinase (New England Biolabs) and purified using G-50 columns ...
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bioRxiv - Molecular Biology 2022Quote: ... 15 pmol dephosphorylated RNA was 5’ phosphorylated with 32P from γ-32P ATP (Hartmann Analytic) with T4 PNK (New England Biolabs) in 1x PNK buffer in a 20 μl reaction ...
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bioRxiv - Molecular Biology 2023Quote: ... Monophosphorylated RNA was synthesized from each of these PCR products in the presence of a 50-fold molar excess of AMP over ATP (74) with T7 RNA polymerase (NEB M0251). Each 50 μL reaction contained 1X reaction buffer ...
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bioRxiv - Plant Biology 2022Quote: ... The specific isotope probe was generated by end-labeling DNA oligonucleotides complementary to the miR167a sequence with [γ-32P] ATP by T4 polynucleotide kinase (New England Biolabs). The level of U6 snRNAs was used as the internal control ...
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bioRxiv - Molecular Biology 2024Quote: ... the linear DNA was digested using 28.8 U/μL of plasmid-safe ATP-dependent DNase Exonuclease V (ExoV, RecBCD) (New England Biolabs, MA, USA) for 1 h at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... The PS oligonucleotide (5’-CGCGGGCCTCTACAACCGGCACCGTG) was synthesized by IDT and 5’-labeled with [γ-32P]ATP and T4 polynucleotide kinase (NEB) to generate the [32P]PS probe ...
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bioRxiv - Microbiology 2024Quote: ... 100 pmol oligonucleotide was incubated with 10 µCi [γ-32P]-ATP in the presence of 10 U T4 polynucleotide kinase (NEB) at 37°C for 2 hours ...
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bioRxiv - Biochemistry 2024Quote: ... 13 nt long RNA was radiolabelled at the 5’-end with [γ-32P] ATP and T4 Polynucleotide kinase (New England Biolabs) prior to complexes assembly ...
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bioRxiv - Biochemistry 2024Quote: ... A 13 nt RNA oligonucleotide was radiolabeled at the 5’ end with [γ-32P] ATP and T4 polynucleotide kinase (New England Biolabs) prior to EC assembly ...
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bioRxiv - Microbiology 2023Quote: ... were assembled by hybridisation of complementary oligonucleotides with one oligonucleotide being radiolabelled with [γ-32P]-ATP by T4 polynucleotide kinase (NEB). 15 µl EMSA samples contained 800 pM DNA template ...
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bioRxiv - Molecular Biology 2023Quote: ... 1/20th of the annealed substrates was 5′-end-labelled with [γ-32P]-ATP and T4 polynucleotide kinase (New England Biolabs). For fluorescently labeled HJ40 substrates ...
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bioRxiv - Cancer Biology 2023Quote: ... then incubated for 2 h at 37°C in 100 μl 1x TdT buffer containing 4 μl 10 mM ATP and 1 μl Terminal Transferase (NEB M0315L). Plugs were rinsed with 1 ml Tris buffer (10 mM Tris HCl (pH 8.0)) ...
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bioRxiv - Plant Biology 2023Quote: ... DNA oligonucleotides complementary to miRNA sequences were end-labeled with γ-32P-ATP using T4 PNK (New England Biolabs, Beverly, MA).
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bioRxiv - Biochemistry 2024Quote: ... Precipitated RNAs were resuspended in Milli-Q water and labeled at the 5’-end with [γ-32P]-ATP using T4 polynucleotide kinase (New England Biolabs). Excess [γ-32P]-ATP was removed by G-25 MicroSpin column (Cytiva ...
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bioRxiv - Synthetic Biology 2024Quote: ... Oligodeoxynucleotides (Table S2) for detection of specific RNA species were end-labeled with [γ- 32P]-ATP using T4 polynucleotide kinase (PNK) (NEB). Prehybridization and hybridization were performed in Church buffer [0.5 M phosphate buffer (pH 7.2) ...
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bioRxiv - Neuroscience 2024Quote: ... and end repair was performed by incubating chromatin-bound beads for 1 h at room temperature in 1× T4 ligase buffer with ATP (NEB, B0202), 0.25 mM dNTPs (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... corresponding to both strands of the tRNA His promoter from positions −45 to +76 or DNA mutants were end labeled using [γ-32P] adenosine 5′-triphosphate (ATP) and T4 polynucleotide kinase (New England Biolabs) and purified on a 10% acryl/bisacrylamide ...
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bioRxiv - Genomics 2024Quote: ... A total of 550 μL of ligation master mix was added: 100 μL of 10× NEB T4 DNA ligase buffer with 10 mM ATP (NEB, B0202), 10 μL of 10 mg/mL BSA (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... The probes were prepared by end labelling 10 pmoles of DNA oligonucleotide complementary to the sfRNA (Supplementary Table 2) with [γ-32P]-ATP (Perkin-Elmer, USA) using T4 polynucleotide kinase (NEB, USA) and purified from unincorporated nucleotides by gel filtration on Illustra MicroSpin G-25 Columns (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... The radioactive probes were prepared by end labelling 10 pmoles of DNA oligonucleotide complementary to the sfRNA (Supplementary table 7) with [γ-32P]-ATP (Perkin-Elmer, USA) using T4 polynucleotide kinase (NEB, USA). Unincorporated nucleotides were then removed by gel filtration on Illustra MicroSpin G-25 Columns (GE Healthcare ...
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bioRxiv - Genomics 2021Quote: The beads were magnetically separated and 4 µl of hot PNK mix (0.2 µl PNK [New England Biolabs], 0.4 µl 32P-γ-ATP, 0.4 µl 10x PNK buffer [New England Biolabs], 3 µl H2O) was added and incubated for 5 min at 37°C in a thermomixer at 1,100 rpm ...
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bioRxiv - Biochemistry 2021Quote: ... to 10 μl of the plasma/stop solution 70 μl of mixture consisting of 75 mU/ml ATP sulfurylase (New England Biolabs, Cambridge, MA), 1 μmol/L APS (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2021Quote: ... The digested DNA (4 ml in total) was split into 4 aliquots and diluted in 8 ml ligation buffer (1X ligation buffer NEB 3 (without ATP), 1 mM ATP ...
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bioRxiv - Biophysics 2024Quote: ... was then 5’-end labeled with 2 pmol of ATP [Ψ32P] (12 uCi/uL, 5000 Ci/mmol, Hartmann Analytic GmbH) using 10 units of T4 polynucleotide kinase (NEB, 10 U/µL) in T4 polynucleotide kinase buffer (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... incubated for 30 min in 100 µl of the end-repair library mix (10 µl of 1x T4 DNA ligase buffer with 10 mM ATP (10x, NEB, cat. no. B0202S), 2 µl of dNTPs (25 mM each) ...
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bioRxiv - Plant Biology 2021Quote: ... DNA oligonucleotides (Table S8) were radiolabelled with [32P]γ-ATP (Hartmann Analytic GmbH, Braunschweig, Germany) using T4 kinase (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... using the manufacturer’s protocol or with radioactive labeling mix (1 µL 10x PNK buffer, NEB, 1 µL of 100 µM gapmer, 1 µL T4 PNK, NEB, 1 µL γ-32P-ATP, Hartmann, 6 µL ddH2O) for 40 min at 37°C ...