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Citations for New England Biolabs :
151 - 200 of 243 citations for Fluorescein alkynylamino atp since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... the top strand (MB733) was 5′ labelled with ϒ[32P]- ATP and T4 polynucleotide kinase (T4 PNK; NEB) for 1 h at 37°C and cleaned up with a G-50 micro column as above ...
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bioRxiv - Genomics 2020Quote: ... 1 mM ATP and 10 mM DTT and with 1 μl (2 U/μl) of RNase H (NEB), 1 μl (3 U/μl ...
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bioRxiv - Molecular Biology 2021Quote: ... γ-phosphate was then removed from excess [γ-32P]ATP with 0.5 U apyrase (New England Biolabs M0398) in 1X apyrase buffer for 10 min at 30°C ...
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bioRxiv - Cell Biology 2020Quote: ... but instead of ATP 1 mM MnCl2 was added and 1 μl (400 U) of λ phosphatase (NEB) was used instead of PKR.
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bioRxiv - Cancer Biology 2020Quote: ... DNA-bound beads were resuspended in 100µl of mix containing 82µl of 1X NEB T4 DNA ligase buffer with 10mM ATP (NEB), 10µl of 10 (2.5mM each ...
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bioRxiv - Microbiology 2020Quote: ... proteins were pre-treated with 0.5 mM ATP and 2.5 U casein kinase 2 (CK2) in 1X CK2 buffer (NEB) for 15 min at 30°C ...
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bioRxiv - Microbiology 2021Quote: ... Promoter DNA fragments were excised from plasmid pSR and end-labelled with γ32-ATP using T4 PNK (NEB). EMSAs and DNase I footprints were done as previously described (42) ...
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bioRxiv - Microbiology 2020Quote: All the templates required for the assay were labelled with [γ-32P] ATP using T4 DNA ligase (NEB) at room temperature for 1 h ...
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bioRxiv - Immunology 2020Quote: ... 1 mM ATP and 3 units of T4 DNA ligase for 2 h at RT (all from NEB). Next ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.2% Tween-20) and RNA 5’ ends where labelled with [γ-32P]-ATP and T4 PNK (NEB, M0201L) at 37°C for 5 min ...
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bioRxiv - Biochemistry 2023Quote: Pre-crRNA was 5’-radiolabeled with [γ−32P] ATP (Perkin-Elmer) using T4 polynucleotide kinase (New England Biolabs). For some measurements ...
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bioRxiv - Biochemistry 2023Quote: ... 13 pmol of purified RNA was phosphorylated with y-32P ATP (Hartmann Analytics) by T4 polynucleotide kinase (NEB) for 30 min at 37 °C ...
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bioRxiv - Microbiology 2023Quote: Promoter DNA fragments were excised from plasmid pSR and end-labelled with γ32-ATP using T4 PNK (NEB). EMSAs and DNase I footprints were done as previously described 40 ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA oligonucleotides complementary to specific RNAs (see supplementary methods) were radiolabeled with [32P]ATP using T4 PNK (NEB), and purified using G-50 columns (Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: The ATP binding mutants (APLFD11A, APLFR37A, APLFK95A) were generated using Q5® Site- Directed Mutagenesis Kit (NEB #E0554) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: ... 1 μL of 100 mM 18O4-γ-ATP followed by addition of 20 U of T4-PNK (NEB) and incubation for 1 h at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... and decapped with 10 U of T4 PNK +/− 40 nmol of ATP and 5 U of RppH (NEB). After each step ...
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bioRxiv - Microbiology 2024Quote: ... prior to end-labeling with γ-32P-ATP in the presence of T4 polynucleotide kinase (New England BioLabs). Single-stranded oligonucleotides were annealed according to established protocols before end-labeling (16) ...
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bioRxiv - Molecular Biology 2024Quote: ... 15 pmol of annealed oligos were end-labeled using γ32P-ATP and T4 Polynucleotide kinase (New England Biolabs) for 1 h at 370C ...
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bioRxiv - Molecular Biology 2023Quote: Internally fluorescein-labelled RNA was produced by ligation of in vitro transcribed 5′ acceptor RNA fragment with chemically produced 3′ donor RNA containing internal fluorescein dT modification and 5′ monophosphate essential for ligase activity using T4 Ligase 2 (NEB #M0239S). Additionally ...
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bioRxiv - Biophysics 2021Quote: ... in 500 μl T4 ligase buffer (50 mM Tris-HCl, 10 mM MgCl2, 1 mM ATP and 10 mM DTT, pH 7.5, NEB). Before adding the ligase ...
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bioRxiv - Molecular Biology 2021Quote: The RNA primer was labelled at the 5’ terminus with [γ-32P]ATP using T4 PNK (New England Biolabs) and PAGE purified ...
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bioRxiv - Microbiology 2021Quote: ... a 39-mer oligonucleotide complementary to the spliced leader was labelled with [γ32P]-ATP using T4 polynucleotide kinase (NEB) and incubated with the membrane overnight at 42°C ...
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bioRxiv - Molecular Biology 2021Quote: ... before incubation during 6 min in 50μl of PNK reaction mix (1x PNKT, 1 mM ATP and 0.05 U/ml T4 PNK 3′phosphatase minus (NEB) in a thermomixer at 37°C and 1400rpm ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the pre-cleared lysates were collected and mixed with the biotin labeled pre-miRNA or biotin labeled shuffled pre-miRNA as negative control in the final concentration of 8.23 ng/ml and ATP (New England Biolabs) in the final concentration of 0.5 mM ...
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bioRxiv - Molecular Biology 2020Quote: ... 5’end of RNA was phosphorylated and labelled by γ-[32P] ATP using T4 Polynucleotide Kinase (New England Biolabs) at 37°C for 45 min ...
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bioRxiv - Microbiology 2022Quote: ... Oligonucleotides complimentary to the target were end-labeled with γ-32P-ATP by T4 polynucleotide kinase (New England Biolabs). Labeled oligonucleotides were added to the membrane and incubated overnight at 45°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Oligonucleotide probe for telomere or Alu repeats was labeled with [32P]-ATP (3,000 Ci/mmol) and T4 nucleotide kinase (New England Biolabs). The membrane was hybridized in Church hybridization buffer containing a 32P-labeled probe at 42°C overnight ...
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bioRxiv - Biophysics 2023Quote: RNAs were labeled with γ32P-ATP using T4 polynucleotide kinase (PNK) according to the manufacturer conditions (New England Biolabs). Radioactive RNAs were separated from any non-incorporated γ32P-ATP via denaturing 15% polyacrylamide (19:1 ...
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bioRxiv - Genomics 2023Quote: ... were mixed in 5 μL of 1x T4 DNA Ligase buffer (50 mM Tris-HCl pH 7.5, 10 mM MgCl2, 1 mM ATP, 10 mM Dithiothreitol; New England Biolabs) at a final concentration of 80 μM each and annealed by heating at 65 °C for 15 min ...
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bioRxiv - Molecular Biology 2023Quote: 20 µM of TOPOVIBLΔC25 monomers purified in the presence of IAA was incubated with 50 µM ATP (P0756L, NEB) or AMP-PNP (64473120 ...
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bioRxiv - Molecular Biology 2022Quote: ... at a final concentration of 0.6 U/μL in CutSmart or rCutSmart Buffer supplemented with 1 mM ATP (P0756, NEB) and 10 mM dithiothreitol (DTT ...
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bioRxiv - Microbiology 2023Quote: ... 0.5 µL of T4 DNA Ligase and 1 µL of 10 mM ATP (all reagents from New England Biolabs, Ipswich ...
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bioRxiv - Microbiology 2024Quote: ... 100 pmol of POWV_Probe and U6_probe DNA probes were radioactively labeled with 5mCi of [γ32-P] ATP using T4 polynucleotide kinase (NEB) for 2 hours at 37°C ...
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: ... Transcription reactions were set up with RNA NTPs (5 mM each ATP, CTP, UTP, 9 mM GTP) (NEB, Ipswitch, MA), 0.004 U/µL Thermostable Inorganic Pyrophosphatase (NEB ...
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bioRxiv - Microbiology 2021Quote: ... or beads alone were suspended in 1x NEBuffer™ for Protein Kinases with 200μM ATP (Thermo) and 100 units of CK2 (NEB) at 30°C for 45 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA-bound beads were re-suspended in 100 µl of mix containing 82 µl of 1X NEB T4 DNA ligase buffer with 10mM ATP (NEB), 10 µl of 10 (2.5mM each ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA-protein complexes were radioactively labeled with 32P-γ-ATP (20 μCi) using 40U T4 Polynucleotide Kinase (New England Biolabs) in supplied PNK buffer for 30 min at 37ºC ...
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bioRxiv - Molecular Biology 2020Quote: Gene-specific primers (Supplementary Table 4) were labeled with [γ-32P]ATP by phage T4 polynucleotide kinase (New England Biolabs), as recommended by the manufacturer ...
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Suppressing STAT3 activity protects the endothelial barrier from VEGF-mediated vascular permeabilitybioRxiv - Molecular Biology 2020Quote: ... to a final concentration of 0.1µg/ml was incubated with 3 µg purified STAT3 proteins as well as 5 µl ATP (New England Biolabs; N0440S) for 30 minutes at 30°C ...
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bioRxiv - Molecular Biology 2020Quote: ... tRF-5s, itRFs were end-labeled with 32P-ATP (Board of Radiation and Isotope Technology, India) using polynucleotide kinase (NEB), purified through MicroSpin G-25 Columns (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: ... The 5’ ends of ds-probes were radioactively labelled with [ψ-32P]-ATP using T4 polynucleotide kinase (New England Biolabs), purified on Illustra Microspin G-25 columns (GE) ...
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bioRxiv - Cell Biology 2020Quote: ... or using 400 U of T4 DNA ligase and 1X reaction buffer (50 mM Tris-HCl, 10 mM MgCl2 1 mM ATP, 10 mM DTT, New England Biolabs) at 16°C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were placed on ice and Ligation Master Mix (1x NEB Ligase Buffer, 1mM ATP, 25% PEG 8000, 15U T4 RNA Ligase I (NEB)) was added to samples ...
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bioRxiv - Biophysics 2023Quote: ... We added the 5’-phospho group on the synthetic parS fragment by adding a T4 kinase for 30 min at 37°C and heat-inactivated 20 min at 65°C in 1x PNK buffer supplemented with 1 mM ATP (T4 PNK, New England Biolabs). Next ...
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bioRxiv - Microbiology 2023Quote: ... and oligonucleotides crp500F and crp500R (one of which was labeled with [γ32P]ATP by use of T4 polynucleotide kinase (NEB)) ...
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bioRxiv - Molecular Biology 2023Quote: Equilibration buffer (GF150 buffer supplemented with 0.1 mM Mg-ATP and 10% glycerol) was used to equilibrate 20 μL of SNAP-Capture Magnetic Beads (New England Biolabs) slurry in 1.5 mL Protein Lo Bind Tubes (Eppendorf) ...
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bioRxiv - Immunology 2023Quote: Circularized DNA templates were prepared by incubating 300 nM of template oligo with 200 nM padlock probe in a 50 μl ligation reaction containing 1mM ATP and 400U of T4 DNA ligase (New England Biolabs) in a buffer comprising 33 mM Tris.acetate (pH 7.9) ...
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bioRxiv - Cell Biology 2024Quote: ... and incubated with 200 µM ATP with ot without 1 µl of recombinant PKA catalytic subunit (PKAc; New England Biolabs) in manufacturer-supplied reaction buffer at 30° C for 30min with agitation ...
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bioRxiv - Cell Biology 2023Quote: ... the beads were incubated in the 30 μl T4 PNK reaction mix (0.3 μl of 0.1 μCi/μl γ-32P ATP, 3U of T4 PNK enzyme (NEB M0201L) in 1X PNK reaction buffer containing 1mM DTT ...