Labshake search
Citations for New England Biolabs :
101 - 150 of 243 citations for Fluorescein alkynylamino atp since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2020Quote: ... Ribonucleotides (ATP, CTP, GTP, and UTP) were purchased from New England Biolabs (NEB). [α–32P] ATP (3000 Ci/mmol ...
-
bioRxiv - Molecular Biology 2021Quote: ... RNAs were 5′-labeled with 32P-γ-ATP using T4 polynucleotide kinase (NEB) and purified on P-30 spin columns (Bio-Rad) ...
-
bioRxiv - Bioengineering 2022Quote: ... cas12a (1 μM) and ATP are purchased from NEB (New England Biolabs Inc). SYBR Green II dye ...
-
bioRxiv - Microbiology 2020Quote: ... Ribonucleotides (ATP, CTP, GTP and UTP) were purchased from New England Biolabs (NEB). [α–32P] ATP (3000 Ci/mmol ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... in the final concentration of 30 ng/ml and ATP (New England Biolabs) in the final concentration of 0.5 mM ...
-
bioRxiv - Microbiology 2022Quote: ... and were initiated by the addition of 500 µM ATP (New England Biolabs).
-
bioRxiv - Developmental Biology 2022Quote: ... 1 mM ATP with 60U of High Concentration T4 RNA ligase (NEB, M0437M) at 16°C overnight ...
-
bioRxiv - Developmental Biology 2020Quote: ... Reaction was stopped by depleting ATP with 4.5 U/ml apyrase (New England Biolabs) for 10 min at room temperature ...
-
bioRxiv - Physiology 2021Quote: ... 5’ end repair was done using T4 PNK with 2 mM ATP (NEB P0756S). Following RNA purification with Zymo Oligo Clean & Concentrator (D4060) ...
-
bioRxiv - Microbiology 2021Quote: ... Fragments were then end-labeled with γ-32P-ATP using T4 polynucleotide kinase (NEB), separated on 15% TBE-Urea gels and visualized by autoradiography ...
-
bioRxiv - Molecular Biology 2020Quote: ... The nucleic acids were subsequently radiolabeled with γ32P-ATP using T4 polynucleotide kinase (NEB) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... followed by labeling with γ-P32-ATP and T4 polynucleotide kinase (Cat#: M0201L, NEB). The reactions were further processed and digested with P1 Nuclease (Cat# ...
-
bioRxiv - Plant Biology 2023Quote: ... 2 μL of 10x T4 DNA Ligase Buffer with 10 mM ATP (NEB, B0202S), 1 μL of PaqCI/AarI activator (5 pmol ...
-
bioRxiv - Plant Biology 2022Quote: ... Probes were end-labelled using [γ-32P]ATP and polynucleotide kinase (New England Biolabs), according to (Sambrook et al ...
-
bioRxiv - Biophysics 2023Quote: ... the buffer was supplemented with 16 mM ATP and RecA (New England Biolabs, USA) as well as SSB gradients were added to the respective rows (Tab ...
-
bioRxiv - Molecular Biology 2023Quote: ... the adaptor complementary primer was labeled with [γ-32P]-ATP and T4 PNK (NEB) using the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1 mM ATP with 60 U of High Concentration T4 RNA ligase (NEB, M0437M) at 16°C overnight ...
-
bioRxiv - Genomics 2021Quote: ... PCR fragments were labeled using 32P-γ-ATP with T4 polynucleotide kinase (New England, Biolabs). Protein–DNA complexes were incubated for 1h at room temperature in binding buffer containing 25 mM Tris pH 7.4 ...
-
bioRxiv - Biochemistry 2021Quote: ... RNA substrates were 5’ end labeled using γ32P-ATP and T4 Polynucleotide Kinase (NEB, MA)38 ...
-
bioRxiv - Biochemistry 2020Quote: ... and subsequently 5’32P-labeled using [γ-32P]-ATP and T4 polynucleotide kinase (New England Biolabs) according to the manufacturer’s protocols.
-
bioRxiv - Genomics 2021Quote: ... consisting of 150 μL 10X NEB T4 DNA ligase buffer with 10 mM ATP (NEB), 125 μL 10% Triton X-100 ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1 mM MgCl2) supplemented with 1 mM ATP and 1 U/μL RNase Inhibitor (NEB). Reaction was incubated for 1 hour at 37°C ...
-
bioRxiv - Genetics 2023Quote: ... Synthetic oligonucleotides were labeled with [γ 32P]-ATP and T4 polynucleotide kinase (New England Biolabs) at their 5’ end ...
-
bioRxiv - Cancer Biology 2022Quote: ... 3 μg purified DARPP-32 isoforms as well as 5 μl ATP (New England Biolabs) in kinase dilution buffer III (SignalChem ...
-
bioRxiv - Plant Biology 2024Quote: ... dephosphorylated F4 (5 µM) was incubated with γ-[32P]-ATP and T4 polynucleotide kinase (NEB) for 45 min at 37°C ...
-
bioRxiv - Microbiology 2023Quote: ... The 5’ ends were radiolabelled with 20 μCi γ32P-ATP using T4 polynucleotide kinase (NEB) and separated from free nucleotides using a MicroSpin G-50 column (Cytiva) ...
-
bioRxiv - Biochemistry 2024Quote: ... the following DNA nucleotide probes were 5’-end 32P-ATP radiolabeled using T4 PNK (NEB): as listed in the Key Resources Table.
-
bioRxiv - Biochemistry 2024Quote: ... 5 pmol were 5′-end-labelled with [γ-32P]-ATP and T4 polynucleotide kinase (NEB). The labeled oligo was mixed in equimolar concentrations with the unlabeled reverse complement and annealed by heating at 100°C in a water bath followed by slow cooling ...
-
bioRxiv - Molecular Biology 2021Quote: ... a portion of eIF3 immunoprecipitated using Dynabeads as described above were treated with nonradioactive ATP (NEB) during the T4 polynucleotide kinase labeling step ...
-
bioRxiv - Biochemistry 2021Quote: ... coli cells (in 10.5 μl of nuclease-free water) were combined with 1 mM ATP (NEB), 40 U RNaseOUT ...
-
bioRxiv - Plant Biology 2021Quote: ... 1 mM ATP and 0.85 U of T4 DNA ligase (67 U/μL) (New England BioLabs). Ligation was performed at 37°C for 2 hours and 16°C for 16 hours ...
-
bioRxiv - Microbiology 2021Quote: ... 1 μL of 10 mM ATP and 1 μL of T4 DNA ligase (New England Biolabs) were added and the ligation reaction was incubated for 5 cycles at 20°C for one hour followed by 37°C for 30 min ...
-
bioRxiv - Genomics 2022Quote: ... Next, 680 nL of ligation mix (1.47x T4 ligase reaction buffer, 5.88 mM ATP (NEB, P0756L), 140 U T4 DNA ligase (NEB ...
-
bioRxiv - Biophysics 2020Quote: ... and were 5’ end-radiolabeled with [Ɣ-32P]-ATP using 10 U T4 polynucleotide kinase (NEB). Radiolabeled transcripts were purified on polyacrylamide gel (6% acrylamide:bisacrylamide 19:1 ...
-
bioRxiv - Genomics 2024Quote: ... and pooled into 7 ml ligation buffer (1X ligation buffer 3 (New England Biolabs; without ATP), 1 mM ATP ...
-
bioRxiv - Genetics 2023Quote: ... All probes were 5’ end-labeled with [alpha-32P]-ATP and T4 polynucleotide kinase (NEB Inc.). The gels were exposed to a PhosphorImager ...
-
bioRxiv - Microbiology 2024Quote: ... All primers were 5’-phosphorylated with T4 polynucleotide kinase using an ATP-containing reaction buffer (NEB). Template backbone DNA was digested with DpnI (NEB) ...
-
bioRxiv - Microbiology 2021Quote: ... Oligonucleotide probes were end-labeled with γ-32P ATP (Perkin-Elmer) and T4 PNK (New England Biolabs) per the manufacturer’s instructions ...
-
bioRxiv - Genetics 2021Quote: ... Ligation buffer is a mixture with final concentrations of 1X T4 DNA Ligase Buffer + ATP (NEB M0202S), 0.01 % TritonX-100 ...
-
Differential impact of a dyskeratosis congenita mutation in TPP1 on mouse hematopoiesis and germlinebioRxiv - Cell Biology 2021Quote: ... 5′ 32P-labeled (TTAGGG)4 oligonucleotide (labeled using [γ-32P]ATP and T4 PNK; New England Biolabs) was added ...
-
bioRxiv - Molecular Biology 2021Quote: An RNA oligonucleotide (5’-GGCATGTGATTGGTGGGTC) was 5’ labelled with gamma 32P ATP by T4 Polynucleotide Kinase (NEB) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... and tRNA-Gly-CCC (Table S1) were radioactively labeled with gamma-32P-ATP using T4 PNK (NEB). Pre-hybridization and hybridization were performed in OligoHyb Buffer (Applied Biosystems ...
-
bioRxiv - Biochemistry 2021Quote: ... and 2 µl of 10x Promega T4 ligase buffer (contains ATP) with 1µl of PNK kinase (NEB) and incubated at 37°C for 1 hour ...
-
bioRxiv - Molecular Biology 2021Quote: ... Table 4 was end labeled using gamma-ATP and T4 Polynucleotide Kinase radioactive labeling protocol from NEB. Labelled oligos were purified using GE Healthcare illustra ProbeQuant G-50 Micro Columns and the membrane was probed overnight ...
-
bioRxiv - Genomics 2023Quote: ... ligation master mix was added (10x NEB T4 DNA ligase buffer with 10 mM ATP B0202-NEB); 10% Triton X-100 ...
-
bioRxiv - Neuroscience 2023Quote: ... 10 µL phosphorylation mix (1µL 100mM sense oligo, 1µL 100mM anti-sense oligo, 0.5µL 25mM ATP (BioLabs #P0756S), 1µL 10X PNK T4 Buffer (BioLabs #B0201S) ...
-
bioRxiv - Cell Biology 2024Quote: ... 20 mL ERA reaction (13 T4 DNA ligase buffer [NEB], 0.5 mM dNTPs, 0.25 mM ATP, 2.75% PEG 4000, 6U T4 PNK [NEB] ...
-
bioRxiv - Immunology 2022Quote: ... 5 mM DTT) prior to incubating with 0.5 μCi/μl [γ32P]ATP and T4 PNK (NEB, M0201L) to radiolabel crosslinked RNA ...
-
bioRxiv - Synthetic Biology 2024Quote: ... was mixed with 0.5 µL of ATP (100 mM) and 1 µL of T4 PNK (NEB, UK) with a 33.5 µL normalized DNA solution (approx ...
-
bioRxiv - Microbiology 2022Quote: ... 5 µl of soluble lysate were incubated at 37 °C for 1 hour with 0.7 µM of a fluorescent ssDNA substrate (5′-ATT ATT ATT ATT CAA ATG GAT TTA TTT ATT TAT TTA TTT ATT T-fluorescein-3′) and 2.5 U of UDG (NEB #M0280) in a total reaction volume of 10 µl (diluted in reaction buffer) ...