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Citations for New England Biolabs :
2401 - 2450 of 4162 citations for 2 4 Methyl 5 thiazolyl ethyl decanoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... 1 μL of 5 μM annealed adaptors and 10 μL of Blunt/TA ligase master mix (NEB) was added to each reaction ...
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bioRxiv - Microbiology 2021Quote: ... and pvhsp70 5’ UTRs were digested using BlpI and SpeI restriction enzymes (New England Biolabs, Ipswich, MA), and agarose gel-purified fragments were subcloned into the plasmid pD-pfcam-Luc also digested with these enzymes ...
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bioRxiv - Genetics 2020Quote: ... RNA was then degraded by the addition of 5 U RNase H (New England Biolabs, cat. M0297S) and cDNA from every 2 reactions was purified on a single RNA Clean & Concentrator-5 column and eluted in 36 μl NF H2O.
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bioRxiv - Cell Biology 2022Quote: ... Hybridized oligonucleotides were phosphorylated with 5 U T4 polynucleotide kinase in T4 Ligation buffer (New England Biolabs) for 30 min at 37 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... Free adaptor was then removed by addition of 1 μL 10 U/μL 5’-Deadenylase (NEB, M0331S). 1 μL 10 U/μL RecJ Exonuclease (Lucigen/Epicentre ...
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bioRxiv - Cell Biology 2021Quote: ... pStr-KDEL_APV-Dendra2/mCherry/EGFP-ER-5 was constructed by the Gibson assembly (New England BioLabs E2611) of pStr-KDEL_SBP-EGFP-Ecadherin linearized by AscI and XbaI ...
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bioRxiv - Microbiology 2020Quote: ... This plasmid was then digested with BamHI and NotI and the resulting insert (5’esaAflank_SpecPromoter_kanR_3’esaAflank) was gel extracted (Monarch DNA Gel Extraction Kit, NEB). 100 ng of purified insert was transformed into S ...
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bioRxiv - Immunology 2021Quote: ... The assembled plasmid pCO2 was transformed into NEB® 5-alpha competent Escherichia coli (High Efficiency) (NEB) according to manufacturer’s instructions (see Supplementary Figure 1 for plasmid maps) ...
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bioRxiv - Synthetic Biology 2021Quote: ... oPool oligos were phosphorylated at their 5’ ends using T4 Polynucleotide Kinase (New England Biolabs, Ipswich, MA). DNA adapters were then annealed to the phosphorylated oPool oligos by incubating extension ...
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bioRxiv - Biophysics 2021Quote: ... 5′-end capping and 3′-end biotinylation were performed using the Vaccinia Capping System (New England Biolabs) and the Pierce RNA 3′ End Biotinylation Kit (Thermo Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... DamID adaptors were removed by digesting overnight at 37° C with 5 units of Sau3AI (R0169S; NEB). The sequencing libraries were prepared according to the Illumina TruSeq nano DNA library protocol ...
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bioRxiv - Genetics 2022Quote: ... The 5’ adaptor (rArCrArCrUrCrUrUrUrCrCrCrUrArCrArCrGrArCrGrCrUrCrUrUrCrCrGrArUrCrU, IDT) was then ligated to the product using T4 RNA ligase 1 (NEB) at 15 °C for 4 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA fragments were directly 3’-end dephosphorylated using 5 U of Antarctic Phosphatase (New England Biolabs, UK) for 30 minutes at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... 0.2 μM reverse primer and 5 μL Luna® Universal Probe qPCR Master Mix (New England Biolabs)] amplified using the Prism7900HT sequence detection system (Applied Biosystems ...
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bioRxiv - Genetics 2022Quote: ... The 37nt long 5’ adaptor was ligated to the small RNAs using T4 RNA ligase (M0204S, NEB) overnight at 16°C ...
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bioRxiv - Genomics 2022Quote: ... was used in a 50 μl-reaction containing 5 μl of NEBuffer r3.1 (10X, NEB, cat# B7203), 26 μl of adapter-ligated DNA and NF water to 50 μl ...
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bioRxiv - Immunology 2022Quote: ... 5 mM DTT) prior to incubating with 0.5 μCi/μl [γ32P]ATP and T4 PNK (NEB, M0201L) to radiolabel crosslinked RNA ...
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bioRxiv - Molecular Biology 2022Quote: miRNAs were synthesized by IDT and radio-labeled at the 5’ end with T4 polynucleotide kinase (NEB) and (γ-32P ...
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bioRxiv - Microbiology 2022Quote: ... followed by adding a 5’ cap using the Vaccina Capping System (cat#M2080S, New England Biolabs Inc.) and a poly-A tail using E.coli Poly(A ...
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bioRxiv - Microbiology 2022Quote: ... 5 ng of α-HA ChIP or input DNA were end-repaired with T4 DNA polymerase (NEB), Klenow DNA polymerase (NEB) ...
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bioRxiv - Neuroscience 2022Quote: ... 45 nM pegRNA3-3AGTGA (Appendix Table S1) and 5 µl NEBuilder HiFi DNA Assembly Master Mix (NEB) and incubated at 37°C for 1 h.
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bioRxiv - Molecular Biology 2022Quote: Approximately 5 μg genomic DNA were digested with 1.5 μl restriction enzymes + 2.5 μl CutSmart buffer (NEB) for 5 hrs at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... 5 μL of PCR product from the inner primer amplification was cleaned using 5μL Exonuclease I (NEB) and 2μL rSAP (NEB) ...
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bioRxiv - Biochemistry 2024Quote: ... which was dephosphorylated at the 5’-end with 3 µl of Quick-CIP (5000 U/µl, NEB) in a total volume of 20 µl according to manufactureŕs instructions ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were amplified using NEB 5-alpha F′Iq Competent Escherichia coli (High Efficiency) (NEB, Cat# C2992H), and isolated with PureYield Plasmid Miniprep System (Promega ...
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bioRxiv - Genomics 2023Quote: ... Reverse transcription reactions (RT +) were then performed with 5 µg of RNA and random hexamers (NEB, #S1330S), using the Superscript III Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... 5 μg of DNA was digested with HinfI and RsaI restriction enzymes (New England BioLabs, 50U each) at 37 °C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... 5’-phosphorylated and dA-tailed with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645). Samples were ligated to adaptor oligos for multiplex sequencing (NEB ...
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bioRxiv - Microbiology 2023Quote: ... pETduet-1::5′-flank_SpecPromoter_kanR_3′-flank and were subsequently digested from the plasmid using BamHI and NotI (NEB). The resultant insert (5′-flank_SpecPromoter_kanR_3′-flank ...
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bioRxiv - Evolutionary Biology 2023Quote: ... containing 12·5 μL of Q5 Hot Start High-Fidelity 2X Master Mix (New England BioLabs, M0494S), 1·1 μL of 10 μM SARS-Cov2-Midnight-1200 primer (either Pool 1 or Pool 2)(17 ...
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bioRxiv - Genetics 2023Quote: ... and amplified for 5 cycles using NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs; M0541L). After evaluation by real-time PCR ...
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bioRxiv - Microbiology 2023Quote: ... CVO689-CVO586 (integrant 5’ end) and CVO321-CVO183 (integrant 3’ end) using Q5 Polymerase (New England Biolabs). PCRs were set up according to manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Beads were treated with JET (12.5 nM d-o-p) and/or ScaI-HF (NEB, 5 U) for 15 minutes at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... 50 µg of protein lysate was treated with 5 µL NeuA (a2-3,6,8,9 Neuraminidase A, P0722L, NEB) or 5 µL of O-Glycosidase (P0733L ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The assembled plasmid was transformed into NEB 5-alpha (high efficiency) competent cells (New England Biolabs; C2987). After verifying the sequence ...
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bioRxiv - Bioengineering 2024Quote: ... Initial studies were done in an alternate NEB® 5-alpha strain (New England Biolabs, Catalog # C2987H) where trpA and trpB were replaced with a chloramphenicol resistance cassette ...
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bioRxiv - Physiology 2024Quote: ... Glycan digestion was achieved with the following enzyme concentrations: 5 μL of α2-3,6,8,9 neuraminidase A (20 U/μL, New England Biolab, NEB) with 1x GlycoBuffer 1 (NEB) ...
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bioRxiv - Genetics 2024Quote: 48 h post-electroporation cells were spun down (300G 5’) and resuspended in DNase I (NEB M0303L) at 37C for 15’ using 10 U of enzyme per ug of plasmid transfected in a final volume of 2 ml of DPBS to eliminate possible carryover plasmid in the exterior of the cells ...
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bioRxiv - Molecular Biology 2024Quote: ... U2OS cells expressing SNAP-H3.1 or SNAP-H3.3 were incubated with 5 µM SNAP-Cell Block (NEB) for 30 min at 37°C for the quench step ...
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bioRxiv - Plant Biology 2024Quote: ... as described in the NEB Golden Gate Assembly protocol (5-10 inserts protocol) (NEB, MA, United States). E ...
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bioRxiv - Molecular Biology 2024Quote: ... the gBlock corresponding to the HIV-1C 5’ LTR was digested with EcoRⅠand BssHⅡ (New England Biolabs), which were also used to digest the pEV731 vector ...
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bioRxiv - Synthetic Biology 2024Quote: ... UK - protocol version ACDE_9163_v114_revO_29Jun2022) and 5 µL of Quick-NEBNext Quick Ligation Module Quick Ligase (NEB, UK) incubated for 10 minutes at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... 5 µl of each sample was mixed with 1 µl of 6X Purple Gel Loading Dye (NEB) and electrophoresed at 110 V for 30 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Embryos were injected with 1 mg of gRNA and 5 μ M of Cas9 protein (NEB, 10128356) at 1-cell stage ...
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bioRxiv - Biochemistry 2024Quote: ... with RBNS RT primer (Supp. Table 5) and PCR amplified via Phusion DNA polymerase (New England Biolabs) with RBNS index primers and RBNS reverse primer (Supp ...
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bioRxiv - Genomics 2022Quote: To digest linear DNA 1 μg of DNA sample was incubated in 50 μl with 1× NEBuffer 4 (NEB), 1mM ATP (NEB ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were centrifuged at 30,597xg and 4°C for 20min and the supernatant was applied to amylose resin (NEB). The column was washed with 10 column volumes (CV ...
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bioRxiv - Biochemistry 2020Quote: ... Vector and fragment were ligated in an overnight reaction at 4 °C using T4 DNA Ligase (New England Biolabs). After transformation ...
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bioRxiv - Cell Biology 2021Quote: ... centrifuged at 58,000 × g for 50 minutes at 4°C and the protein was batch purified using chitin beads (NEB). Protein-bound chitin beads were washed with lysis buffer and high salt buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Plant Biology 2020Quote: ... The region of interest was amplified with specific primers (see Supplemental Table 4) and the Q5 DNA polymerase (NEB), and indexed by PCR using primers containing Illumina indexes (see Supplemental Table 4) ...