Labshake search
Citations for New England Biolabs :
2251 - 2300 of 4162 citations for 2 4 Methyl 5 thiazolyl ethyl decanoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... and then the extracted RNA was treated with 2 units of DNase I (NEB) and further cleaned up via phenol-chloroform (pH 4.3 ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 15 μL of 2× Phusion High-Fidelity PCR Master Mix (New England Biolabs). All PCR amplification reactions were carried out on a T100 Thermal Cycle (Bio-Rad ...
-
bioRxiv - Molecular Biology 2020Quote: ... Barcoded pre-adenylated linkers were ligated using T4 RNA ligase 2 truncated K227Q (NEB) and rRNA was depleted using the Ribo-Zero gold kit (Illumina ...
-
bioRxiv - Neuroscience 2020Quote: ... Sections were quenched with 2% H2O2 and digested in 10µg/ml Proteinase K (BioLabs). The reaction was stopped with 2mg/ml Glycine (Sigma ...
-
bioRxiv - Plant Biology 2020Quote: ... The nuclei were washed two times with a plastid-lysis buffer (NEB + 2% triton) followed with centrifugation (5 min at 1500 g ...
-
bioRxiv - Bioengineering 2020Quote: ... half of the DNA was mixed with 2 μL ExoI (M0293S, New England Biolabs), 1 μL of Lambda Exonuclease (M0262S ...
-
bioRxiv - Bioengineering 2020Quote: ... 2 μL of PCR mixture was used for 10 μL KLD reaction (NEB, MA), which proceeded under room temperature for 1 hour ...
-
bioRxiv - Cancer Biology 2020Quote: ... and NEBNext Multiplex Oligos for Illumina (NEB Set 1 E7335 and Set 2 E7500S). Additional AMPure clean-ups at the start and the end of library preparation were included ...
-
bioRxiv - Plant Biology 2021Quote: ... for level 2 reaction together with T4 DNA Ligase (New England BioLabs, Ipswich, UK). TCSn promoter DNA sequence (Zürcher et al. ...
-
bioRxiv - Biochemistry 2021Quote: ... and 2 µg DNA was treated with uracil DNA glycosylase (New England Biolabs, Inc.) at 37 °C for 60 min ...
-
bioRxiv - Systems Biology 2021Quote: ... 2 micrograms of each library was digested with SphI-HF and SpeI-HF (NEB) and then treated with Antarctic phosphatase (NEB).
-
bioRxiv - Systems Biology 2020Quote: ... Samples were digested using 2 Units/μL MNase in 20μL MNase Digestion Buffer (NEB) for 5 minutes at 37°C ...
-
bioRxiv - Developmental Biology 2021Quote: ... for 10 min with 2 mM Ribonucleoside-Vanadyl Complex RVC (New England Biolabs, S1402S) at room temperature and then permeabilized for 5 min on ice in 0.5% Triton-X with 2mM RVC ...
-
bioRxiv - Plant Biology 2021Quote: ... for 3 hours at 55°C and M-2 was digested with XhoI (NEB) overnight at 37°C ...
-
bioRxiv - Cell Biology 2021Quote: ... and cloning was performed using Gibson Assembly 2× Master Mix (New England BioLabs, NEB) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... and cloning was performed using Gibson Assembly 2× Master Mix (New England BioLabs, NEB) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... About 2-5μg of fragmented DNA was digested with 40U RNaseH (New England Biolabs) for at least 24 hours at 370C to serve as a negative control ...
-
bioRxiv - Molecular Biology 2022Quote: pLXV-EF1alpha-2xStrep-SARS-CoV-2-nsp14-IRES-Puro was opened with BstBI (NEB) and AfeI (NEB) ...
-
bioRxiv - Microbiology 2020Quote: ... cells were incubated with 2 μM SNAP-Cell® 647-SiR (New England Biolabs) for 30 min at 37 °C ...
-
bioRxiv - Molecular Biology 2021Quote: ... 2 μg of the DNA substrate were digested with 30U of Nb.BbvCI (from NEB) for 2 h at 37 °C ...
-
bioRxiv - Immunology 2021Quote: ... 2 µL of 0.1 M Dithiothreitol (cat. n° M0368L, NEB, New England Biolabs, USA), 1 µL of 10 mM dNTP (cat ...
-
bioRxiv - Immunology 2021Quote: ... 2 µL of 0.1 M Dithiothreitol (cat. n° M0368L, NEB, New England Biolabs, USA), 1 µL of 10 mM dNTP (cat ...
-
bioRxiv - Microbiology 2020Quote: ... The content was mixed with 2 μL of denaturing buffer (New England Biolabs™) and 2 μL of endo-β-N-acetylglucosaminidase-H (Endo-H ...
-
bioRxiv - Neuroscience 2021Quote: ... Point mutants in the aman-2 cDNA were introduced by site-specific mutagenesis (NEB Q5 Site-Directed
-
bioRxiv - Microbiology 2022Quote: ... and 1 μl T4 RNA ligase 2 (truncated K227Q, NEB, M0242, 200,000 units/ml) and incubated at 16°C for 14 h ...
-
bioRxiv - Molecular Biology 2020Quote: ... PNK was used to dephosphorylate RNA for 2 hours (New England Biolabs, Ipswich, MA) followed by heat-inactivation ...
-
bioRxiv - Molecular Biology 2020Quote: ... Resulting PCR amplicons were denatured and re-annealed in 1 × NEB buffer 2 (NEB) in a total volume of 9 μl using a following conditions ...
-
bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2-bound proteins were eluted by adding 7.5 μl RNase H (NEB), 2 μl TURBO DNase (Thermo Fisher Scientific ...
-
bioRxiv - Genomics 2021Quote: ... 2 ul of the library was loaded using NEB loading dye (Cat: NEB #B7025) because it did not make a shadow on the gel as compared to the bromophenol blue dye ...
-
bioRxiv - Microbiology 2020Quote: ... Chromatin was digested overnight (ON) with either 2 μl (100 U) Dpn II (NEB) (later experiments ...
-
bioRxiv - Biochemistry 2020Quote: ... the DNA template was digested with 2 units of DNase I (New England Biolabs) for 1 h at 37 °C and the RNA was desalted twice by Illustra Microspin G-25 columns (GE Healthcare Life Sciences) ...
-
bioRxiv - Developmental Biology 2020Quote: ... and then digested by 2 U of T7 endonuclease I (T7E1, New England BioLabs) at 37 °C for 15 min ...
-
bioRxiv - Cell Biology 2020Quote: The pIGLR-2∷mCherry construct was generated with a Gibson assembly cloning kit (NEB) with the following four fragments ...
-
bioRxiv - Biochemistry 2020Quote: ... the supernatant was collected and passed through a 2 mL Amylose resin (NEB E8021S) pre-equilibrated with lysis buffer ...
-
bioRxiv - Molecular Biology 2021Quote: ... The reverse transcriptase linker was then ligated with T4 RNA Ligase 2 truncated (NEB) and reverse transcribed using M-MLV RNase H minus (Promega) ...
-
bioRxiv - Biophysics 2021Quote: ... was generated by subcloning Mdn1 aa4381-4717 into pSNAP-tag(T7)-2 (NEB N9181S) downstream of the SNAP tag ...
-
bioRxiv - Biochemistry 2022Quote: ... 2 and cloned into pET28a using T4 DNA ligase (New England Biolabs GmbH, Germany). The gene encoding ribose-phosphate pyrophosphokinase (RPPK ...
-
bioRxiv - Bioengineering 2022Quote: ... Samples requiring dephosphorylation were treated with 2 U/μL λPP (New England Biolabs, # P0753) for 3 h at 30°C and then washed with PBSCM ...
-
bioRxiv - Cancer Biology 2022Quote: ... DNase I (2 µL, 2,000 U/ml, RNase-free, New England Biolabs, Ipswich, MA), 10× DNase buffer ...
-
bioRxiv - Molecular Biology 2023Quote: ... and inserted into the pGLuc Mini-TK 2 Gaussia luciferase enhancer reporter plasmid (NEB) via KpnI/SacI restriction sites ...
-
bioRxiv - Neuroscience 2022Quote: ... and nuclei were resuspended in 2 mL 1x NEBuffer 3.1 (New England Biolabs; B7203S) + 20uL NxGen RNase Inhibitor.
-
bioRxiv - Molecular Biology 2022Quote: ... were ligated to dephosphorylated RNAs using T4 truncated RNA ligase 2 (K227Q) (NEB, #M0351L). Unligated linkers were depleted by using 5 U per sample of 5’ deadenylase (NEB ...
-
bioRxiv - Microbiology 2022Quote: ... Secondary amplification reactions were performed using NEBNext high-fidelity 2 PCR master mix (NEB) in 50 μl reactions (26 μl of 2X mix ...
-
bioRxiv - Microbiology 2022Quote: ... The resulting nucleic acids were treated with 2 U DNase I (New England Biolabs), 10 U S1 nuclease (Thermo Scientific ...
-
bioRxiv - Molecular Biology 2024Quote: ... 20 µL of reactions were treated with 2 µL DNase I (NEB, Cat # M0303S) in a total volume of 100 µL at 37°C for ∼ 20 min before further analysis and treatment.
-
bioRxiv - Immunology 2023Quote: ... and 2 µL of home-made Gibson mix (T5 exonuclease (0.2U; New England Biolabs), Phusion polymerase (12.5U ...
-
bioRxiv - Cell Biology 2024Quote: ... all kinases were tested for activity using 2 µg each of histone H1 (NEB) or Myelin Basic Protein (MBP ...
-
bioRxiv - Developmental Biology 2024Quote: ... R0148S)2 or the wild-type allele was digested with HphI (New England Biolabs, R0158S) for 1 hour ...
-
bioRxiv - Genomics 2024Quote: ... and mixed with an equal volume of 2 x RNA loading dye (NEB, B0363S). 25 μl of the mixture was loaded on a 10% TBE-Urea gel (Bio-Rad ...
-
bioRxiv - Genomics 2023Quote: ... 20 of 2 U µL-1 Phusion High-Fidelity DNA Polymerase (New England Biolabs), 160 µL of 40 U µL-1 Taq DNA ligase (New England Biolabs ...