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Citations for New England Biolabs :
2301 - 2350 of 4162 citations for 2 4 Methyl 5 thiazolyl ethyl decanoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The reaction with T4 RNA ligase 2 was performed in 1x T4 Rnl2 (NEB) supplemented with PEG 8000 (10% ...
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bioRxiv - Molecular Biology 2023Quote: ... or NEBNext Ultra II DNA Library Prep Kit for Illumina (replicate 2: NEB, E7645S), according to the manufacturers’ protocols ...
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bioRxiv - Plant Biology 2023Quote: ... 2 μL of 10x T4 DNA Ligase Buffer with 10 mM ATP (NEB, B0202S), 1 μL of PaqCI/AarI activator (5 pmol ...
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bioRxiv - Physiology 2023Quote: ... for every 2 µg of fusion protein in 1X TEV buffer (Catalog P8112S, NEB) for 16h at 30°C.
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bioRxiv - Cell Biology 2023Quote: ... The eluted sample was incubated with 2 ml amylose resin (New England Biolabs, E8021L) for 1 h at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... 10 μL PCR reactions were set-up using 2 μL 5x HF Buffer (NEB), 0.5 μL 10 mM dNTPs (NEB) ...
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bioRxiv - Biophysics 2023Quote: ... 2 pmol were digested to single nucleosides using the Nucleoside Digestion Mix (NEB # M0649), and the ratio of Ψ s versus uridines was determined via tandem quadrupole mass spectrometry (Supplementary Figure S2a).
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bioRxiv - Microbiology 2023Quote: ... 20-μL reactions contained 2 μM enzyme and 250 μM NTPs (New England BioLabs) in reaction buffer with 50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was ligated with 3′-adenylated adapters using T4 RNA Ligase 2 (NEB #MO351) for 4 hrs at 16°C ...
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bioRxiv - Microbiology 2023Quote: ... The annealed constructs were ligated using 2 µL T4 RNA ligase2 (NEB, 10U/µL), 3 µL 0.1% BSA (Ambion) ...
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bioRxiv - Molecular Biology 2023Quote: ... two pairs of oligonucleotides (Suppl. Table 2) were phosphorylated and ligated into BsbI (NEB) digested pDG459 plasmid ...
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bioRxiv - Molecular Biology 2023Quote: ... a pair of oligonucleotides (Suppl. Table 2) was phosphorylated and ligated into BsbI (NEB) digested pX459 and pX458 plasmids ...
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bioRxiv - Genomics 2023Quote: ... Forked adapters (Supplementary Table S7) were annealed in NEBuffer 2 1X (NEB cat. B7002S) to a final concentration of 20 µM in a thermal cycler set to heat at 98°C and gradually cool down to 4°C with a 1°C per 20 sec gradient.
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bioRxiv - Immunology 2024Quote: ... washed in 10 mL RPMI+10% FBS + 2 µl RNase-free DNase I (NEB), and stained with LIVE/DEAD Aqua for 15 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... washed in 10 mL RPMI+10% FBS + 2 µl RNase-free DNase I (NEB), and placed in single-cell suspension in 1 mL RPMI+10% FBS ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 μM of dsDNA was incubated with CpG methyltransferase (M. SssI, New England Biolabs) in the presence of 200 μM of S-adenosylmethionine ...
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bioRxiv - Genetics 2024Quote: ... the DNA-bound beads were digested with 2 μl BpmI (NEB, catalog number R0565L) for 30 min at 37 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... Libraries were generated via PCR amplification using NEBNext HighFidelity 2× PCR Master Mix (NEB). The resultant libraries were purified again using the MinElute PCR Purification Kit (Qiagen) ...
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bioRxiv - Genomics 2024Quote: ... 0.4 µL RNase Inhibitor and 2 µL Induro RT (200 U/µL; NEB M0681S) were incubated for 15 minutes at 60°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Sequencing libraries were prepared with NEBNext High-Fidelity 2×PCR Master Mix (NEB, M0541S).
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bioRxiv - Synthetic Biology 2024Quote: ... 2 µL of the annealed product were mixed with 0.5 U Klenow Fragment (NEB), 33 µM dNTPs ...
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bioRxiv - Molecular Biology 2024Quote: ... in 100 μl final volume per coverslip using: 2 μl T4 Ligase (NEB; #M0202S), 5 μl 10 μM biotinylated linker (5′-TACTACCTCGAGAGTTACGCTAGGGA-TAACAGGGTAATATAGTTT[biotin– dT]TTTCTATATTACCCTGTTA-TCCCTAGCGTAACTCTCGAGGTAGTA-3′) ...
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bioRxiv - Molecular Biology 2024Quote: ... The digested nuclei were ligated with 2 μl of T4 DNA ligase (NEB M0202S) in a total ligation volume of 2 ml at 16°C for 12 h.
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bioRxiv - Biochemistry 2024Quote: ... 1 µL of Buffer 2 and 0.5 µL of rSAP (New England Biolabs, M0371S) pre-mixed with 0.5 µL of 50% glycerol were added ...
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bioRxiv - Genomics 2024Quote: ... DNase I (2 µL, 2,000 U/ml, RNase-free, New England Biolabs, Ipswich, MA), 10× DNase buffer ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were recovered in 2 mL of 37°C SOC recovery medium (NEB #B9020S) at 37°C for 1 hour ...
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bioRxiv - Biophysics 2024Quote: ... Vector DNA was prepared by digestion with EcoRI (NEB, R3101S, 2 unit/μg DNA) and HindIII (NEB ...
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bioRxiv - Bioengineering 2024Quote: ... USER enzyme digestion: 500 ng aliquots were digested with 2 µL USER Enzyme (NEB) at 37°C for 3 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... and 2 µl of T4 DNA ligase (2,000,000 U/ml, NEB, cat. no. M2200M), for 15 min at RT with 3 µl of the USER adaptor (NEBNext Multiplex Oligos for Illumina ...
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bioRxiv - Genomics 2020Quote: ... CpG dinucleotides were methylated by incubating 1µg of DNA with S-Adenosyl methionine (SAM) (32µM) with CpG Methyltransferase (M.SssI) (4-25 units) (New England BioLabs) at 37°C for 1h before heating to 65°C for 20mins.
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bioRxiv - Genomics 2020Quote: ... by incubating 1µg of DNA with S-Adenosyl methionine (SAM) (32µM) with CpG Methyltransferase (M.SssI) (4-25 units) (New England BioLabs) at 37°C for 1h before heating to 65°C for 20mins.
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bioRxiv - Cell Biology 2020Quote: ... The extract was incubated for 1 h at 4 °C with amylose resin (New England Biolabs, Ipswich, MA), loaded onto a column ...
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bioRxiv - Systems Biology 2020Quote: Single hematopoietic stem and progenitor cells were index-sorted into 384 well plates containing 0.5 µl of nuclease free water with 0.2% Triton-X 100 and 4 U murine RNase Inhibitor (NEB), spun down and frozen at −80°C ...
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bioRxiv - Systems Biology 2021Quote: ... Then a plasmid library was assembled using 4 independent reactions of NEBuilder HiFi DNA Assembly (New England Biolabs) to avoid biases in assembly that might affect the library’s distribution ...
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bioRxiv - Biochemistry 2022Quote: ... The unbound fraction was then incubated for 1 hour at 4 °C with lambda phosphatase (New England Biolabs), calf intestinal phosphatase (New England Biolabs) ...
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bioRxiv - Cell Biology 2022Quote: ... pEXKm5 was digested with HindIII and the 4 fragments were assembled via Gibson cloning (New England Biolabs, E2611S).
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bioRxiv - Microbiology 2022Quote: ... DNA was synthesized from extracted RNA using 4 μL LunaScript RT SuperMix 5X (New England Biolabs, NEB, USA) and 8 μL nuclease free water ...
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bioRxiv - Microbiology 2022Quote: ... DNA was synthesized from extracted RNA using 4 μL LunaScript RT SuperMix 5X (New England Biolabs, NEB, USA) and 8 μL nuclease free water ...
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bioRxiv - Neuroscience 2021Quote: ... nuclei were resuspended in 200uL digestion buffer with 4 uL protease (an equal volume of protease K (NEB) was substituted if the manufacturer-provided protease was exhausted ...
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bioRxiv - Molecular Biology 2020Quote: ... the single cell tagmentation product was mixed well with 4 units of Bst 3.0 DNA Polymerase (NEB, Cat.No.M0374) and indexed common primers (Vazyme ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 million permeabilized cells (without any antibodies bound) were incubated with 4 units of Dam enzyme (NEB, M0222L) during the activation step ...
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bioRxiv - Genomics 2021Quote: ... We then performed a kinase treatment adding 4 μl of T4 Polynucleotide Kinase (PNK, New England BioLabs Inc.), 5 μl of 10X PNK buffer ...
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bioRxiv - Genomics 2021Quote: The beads were magnetically separated and 4 µl of hot PNK mix (0.2 µl PNK [New England Biolabs] ...
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bioRxiv - Genomics 2021Quote: The beads were magnetically separated and resuspended in 20 µl of 3’ end RNA dephosphorylation mixture (4 µl 5x PNK pH 6.5 buffer, 0.5 µl PNK [New England Biolabs; with 3’ phosphatase activity] ...
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bioRxiv - Synthetic Biology 2021Quote: ... annealed by temperature decrease from 95 °C to 4 °C and phosphorylated using T4 Polynucleotide Kinase (NEB, M0201) according to manufactures’ protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... for 12 h at 4 °C with 10 μg of Factor Xa protease (New England Biolabs, Hitchin, UK) per 1 g of mitochondria ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting Gβ1γ2 was then incubated for 1 hour at 4 °C with lambda phosphatase (New England Biolabs), calf intestinal phosphatase (New England Biolabs) ...
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bioRxiv - Genomics 2021Quote: ... Nicks were then ligated for 30 min at 37 °C using 4 units of Taq DNA ligase (NEB) in the presence of 0.5 μl thermopol buffer ...
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bioRxiv - Immunology 2021Quote: ... into 384 well plates containing 0.5 µl of nuclease free water with 0.2% Triton-X 100 and 4 U murine RNase Inhibitor (NEB), spun down and frozen at −80°C ...
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bioRxiv - Biophysics 2020Quote: ... SecA(N95) K797C was overexpressed for 4 hours at 37 °C in E.coli NiCo21 cells (New England Biolabs) grown in 2xYT (Acumedia ...