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Citations for New England Biolabs :
2301 - 2350 of 6344 citations for 6H Dibenzo b d pyran 3 pentanaminium 6a 7 10 10a tetrahydro 1 hydroxy N N N 6 6 9 hexamethyl 6aR trans 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: The standard HiDEF-seq v2 protocol was followed with the exception of replacing Klenow fragment 3’→5’ exo-polymerase with one of the following: 9.6 U Bst large fragment (NEB), 9.6 U Bst 2.0 (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... The samples were then incubated at 42°C overnight with the addition of 3 μl of β-agarase (NEB). The DNA mix was then gently poured into combing reservoirs containing 1.2 ml MES and the genomic DNA was combed onto salinized coverslips (Genomic Vision ...
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bioRxiv - Molecular Biology 2023Quote: ... The extracted chromatin was digested for 3 hours at 37 °C in a volume of 250.0 μL with 100 U of NlaIII (NEB) for 3C library preparation ...
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bioRxiv - Microbiology 2023Quote: ... Fragmented RNAs were then dephosphorylated at their 3’ end using T4 Polynucleotide kinase (PNK, New England Biolabs, Cat: M0201) in MES buffer (100 mM MES-NaOH ...
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bioRxiv - Cancer Biology 2023Quote: ... adenosines were added to the 3′ ends of dsDNA and adapters were ligated (adapters from NEB, Ipswich, MA, USA). Following the adapter ligation ...
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bioRxiv - Biochemistry 2023Quote: ... The clarified supernatant was then batch-bound for 3 hours on to 20 mL chitin resin (New England Biolabs) equilibrated with 200 mL of cell lysis buffer ...
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bioRxiv - Genomics 2023Quote: ... 5 µg of genomic DNA was dephosphorylated by 3 µL of Quick Calf Intestinal Phosphatase (CIP, New England Biolabs) in a total volume of 30 µL for 10 min at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... a DNA adapter (TableS6) was ligated to 3′ ends of nascent RNA using the T4 RNA ligase kit (NEB) by mixing 50 pmol adapter with 300-600 ng nascent RNA ...
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bioRxiv - Genetics 2023Quote: ... 100 ng of genomic DNA was digested with 5U of BtsCI in a 3 μl reaction (New England Biolabs), 1 hour at 50°C incubation and no enzyme denaturation ...
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bioRxiv - Microbiology 2023Quote: ... hysA (SAA6008_RS12255) and vigR 3’ UTR from JKD6008 were in vitro transcribed (IVT) using HiScribe T7 RNA polymerase (NEB). IVT products were RQ1 DNase treated (Promega ...
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bioRxiv - Microbiology 2023Quote: ... the 5’-PPP RNA in the total RNA was specifically capped with 3’-Desthiobiotin-GTP (New England BioLabs, N0761) by the Vaccinia Capping System (New England BioLabs ...
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bioRxiv - Plant Biology 2024Quote: ... This end-repaired DNA was subjected to A-tailing using the Klenow 3′–5′ exo− enzyme (New England Biolabs). The subsequent step involved the ligation of methylated adapters to the A-tailed DNA ...
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bioRxiv - Bioengineering 2023Quote: ... and assembled with PCR amplified (primers 2 and 3) pRSET vector fragment using Gibson assembly (NEB Japan, Tokyo, Japan) to add T7 promoter ...
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bioRxiv - Cell Biology 2023Quote: ... and the newly formed CENP-A was pulse labelled with 3 μM SNAP-Cell® 647-SiR (S90102S, NEB), 2 h after release from the STLC block (early G1) ...
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bioRxiv - Genomics 2024Quote: ... Two cycles of whole genome amplification were performed using 50 U of Klenow Fragment (3’→5’ exo-) (NEB, M0212M), dNTP solution mix (Bio-Rad ...
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bioRxiv - Developmental Biology 2024Quote: ... 2020) and 5’Ncol1::mScarlet::3’Ncol119 plasmids using Gibson assembly according to the manufacturer recommendations (NEB, CAT#E2611). Sequences of Gibson primers used were:
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bioRxiv - Genomics 2024Quote: M13seq primer (5’-GACGTTGTAAAACGACGGC-3’) was labeled with 32P at 5’-end by T4 polynucleotide kinase (New England Biolabs). Primer/template (p/t ...
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bioRxiv - Neuroscience 2024Quote: ... incubated at 37°C for 12 minutes and then washed with 0.1 3 SSC buffer (Thermo, AM9770) supplemented with 0.05 U/ml RNase inhibitor (NEB, M0314L). RNA released from the permeabilized tissue and captured by the DNB was reverse transcribed overnight at 42C using SuperScript II (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... 3 µL of the reverse phasing primer pool (100 nM) and 15 µL of Q5 Mastermix (New England Biolabs). Cycle conditions were 4 minutes at 98°C followed by 20x (30 seconds at 98°C ...
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bioRxiv - Molecular Biology 2024Quote: ... A customized linker (rand10_3Tr3) was added to the 3’ end of on-bead LAM products using T4 RNA ligase I (M0204S, NEB). Nested PCR was performed using indicated primer set (3Tr3-Adaptor_R and JM_F ...
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bioRxiv - Genomics 2022Quote: ... 1 μl of Exonuclease 1 (NEB, M0293S) was added to each PCR product and incubated at 37C for 15 min to digest any single-stranded product ...
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bioRxiv - Molecular Biology 2024Quote: ... in 1× NEB buffer 1 (NEB, B7001S) for 15 min at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... and Adapter Ligated using custom reagent formulations (New England BioLabs, E6000B-10) and in-house prepared Illumina forked small adapter ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mU of phosphodiesterase II from Sigma (# P9041-10 UN) and 2 U of alkaline phosphatase from Biolabs (# M0290) were added and the mixture was incubated at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... 13 μl of 10× NEBuffer3.1 and 100 units of MboI (NEB, R0147M) were added and the chromatin was digested at 37 °C overnight while shaking ...
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bioRxiv - Genetics 2019Quote: ... Clones were transformed into electrocompetent 10-Beta cells (New England Biolabs # C3020) using a 96-well plate electroporator using fresh electroporation plates (BTX 45-0450-M) ...
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bioRxiv - Genomics 2019Quote: ... gDNA was pre-treated with SAP (10 U, New England Biolabs #M0371S) in CutSmart buffer in a total volume of 10 µl at 37 °C for 1 h ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 2ul of 10 x T4 DNA Ligase Buffer (New England Biolabs # M0202S), 1ul (400Units ...
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bioRxiv - Genomics 2021Quote: ... 12 μL of 10 mg/mL Bovine Serum Albumin (100× BSA, NEB), 5 μL of 400 U/μL T4 DNA Ligase (NEB M0202) ...
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bioRxiv - Microbiology 2021Quote: ... by incubating with 10 U of T4 polynucleotide kinase (New England Biolabs) at 37°C for 1 h ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 μl of 10 U/μl T4 polynucleotide kinase (NEB; Cat#: M0201L), 1 μl of 5 U/μl Klenow DNA polymerase ...
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bioRxiv - Cell Biology 2021Quote: ... an identical labeling scheme was followed using CLIPcell block (10 µM; NEB) and CLIPcell-TMR (1 µM ...
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bioRxiv - Biochemistry 2020Quote: ... and 50 µL of 10 µM H3/H4 tetramer (New England BioLabs). The NaCl concentration was gradually lowered by adding increasing volumes of 10 mM Tris HCl (pH 8 ...
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bioRxiv - Genetics 2020Quote: ... the eluent was mixed with 10 μl 10x T4 ligation buffer (NEB), 10 μl 100 mM ATP ...
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bioRxiv - Microbiology 2021Quote: ... to create a single double-stranded cut or 10 U Nb.BtsI (NEB) to create a single nick ...
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bioRxiv - Molecular Biology 2022Quote: ... and 10 units of T4 polynucleotide kinase (PNK) (New England Biolabs, #M0201S) in PNK buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... and adapter ligated using custom reagent formulations (New England BioLabs, E6000B-10) and in-house prepared Illumina forked small adapter ...
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bioRxiv - Molecular Biology 2020Quote: ... 180 μM dNTPs and 10 units of phi29 DNA polymerase (NEB, M0269S) and incubating at 30°C for 20 min ...
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bioRxiv - Microbiology 2021Quote: ... Fragmented RNA was treated with 10 U of T4 polynucleotide kinase (NEB) in 20 μl reactions containing 1 mM ATP ...
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bioRxiv - Biophysics 2021Quote: ... the library was transformed into elec-trocompetent NEB 10-beta cells (NEB) to yield ¿1000x transformants per gene ...
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bioRxiv - Genetics 2022Quote: ... and 10 µL NEBNext Quick T4 DNA Ligase (NEB, Ipswich, MA, USA) to the separate tubes ...
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bioRxiv - Immunology 2022Quote: ... a quantitative PCR reaction (10 µl 2 x PCR master mix (NEB), 1 x SYBR green (Thermo Fisher) ...
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bioRxiv - Genetics 2019Quote: ... and transformed into high efficiency 10-beta competent E.coli (New England BioLabs) to generate pDestTol2pA2_ubi:f5-p2A-EGFP ...
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bioRxiv - Molecular Biology 2019Quote: ... 10–20% were sequentially digested with a mix of restriction enzymes (NEB) according to their buffer requirements and temperatures ...
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bioRxiv - Synthetic Biology 2021Quote: ... NEB 10-beta/Stable Outgrowth Medium (450 μL; B9035S, New England Biolabs) was added to each aliquot and the mixture was shaken vigorously (1250 rpm ...
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bioRxiv - Molecular Biology 2021Quote: ... gDNA was pre-treated with SAP (10 U, New England Biolabs #M0371S) in CutSmart buffer in a total volume of 10 μl at 37°C for 1h ...
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bioRxiv - Genomics 2019Quote: ... The library was then transformed into 10-beta electrocompetent cells (NEB C3020), grown in 100 mL of LB+Amp ...
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bioRxiv - Genomics 2021Quote: ... and 10 μL 5U/μL DNA Pol I Large Klenow fragment (NEB), then incubated at 37 °C for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: ... the protein was batch bound to 10 mL of chitin resin (NEB) for 1 hour at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Ligation master mix (398 μL of water, 50 μL of 10 × NEB T4 DNA ligase buffer ...