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Citations for New England Biolabs :
2251 - 2300 of 6344 citations for 6H Dibenzo b d pyran 3 pentanaminium 6a 7 10 10a tetrahydro 1 hydroxy N N N 6 6 9 hexamethyl 6aR trans 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 20 mM MgCl2 and digested with 10 μg DNAse I (NEB) and 50 μg RNAse (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... 2 mM DTT) and a wash with 10 μL streptavidin (NEB). The chamber was further washed with 10 μL TIRF buffer followed by a 1-minute incubation with 2 μL of microtubules diluted in 8 μL TIRF buffer supplemented with 50 mM KCl and 1.25 mg/mL casein (TIRF-Casein) ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.5% SDS) with 10 µg proteinase K (New England Biolabs, P8107S), vortexed briefly ...
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bioRxiv - Synthetic Biology 2023Quote: ... in 1x T4 DNA ligase buffer with 10 µM ATP (NEB) in 10 µL reactions ...
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bioRxiv - Bioengineering 2024Quote: ... PCR reactions were composed of 10 µL Q5 reaction buffer (NEB), 1 µL 10 mM dNTPs (NEB) ...
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bioRxiv - Cell Biology 2022Quote: ... adding 10 μM SNAP-Cell TMR-Star dye (New England Biolabs) and incubating for 1 h at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... and cleaned up with Monarch RNA Cleanup Kit (10 ug) (NEB). These cleaned RNA samples were sent to Novogene for poly-A tail enrichment mRNA library preparation and 150 bp paired end sequencing on Illumina NovaSeq (Novogene ...
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bioRxiv - Immunology 2022Quote: ... and 10 μl 2X Luna Universal One-step Reaction mix (NEB). Samples were measured in triplicates ...
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bioRxiv - Cell Biology 2023Quote: ... 10 µL Q5® Reaction Buffer (New England BioLabs, Inc., B9027SVIAL), 10 µL Q5® High GC Enhancer (New England BioLabs ...
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bioRxiv - Biochemistry 2023Quote: ... The dsDNA substrate was a ~10-kb Nt.BbvCI-nicked (NEB #R0632) pMYC plasmid used at 2.5 ...
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bioRxiv - Biochemistry 2023Quote: ... the dsDNA substrate was a ~10-kb Nt.BbvCI-nicked (NEB #R0632) pMYC plasmid used at 5 ng/µL (~0.83 nM) ...
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bioRxiv - Microbiology 2023Quote: ... which contained 10 μL Luna Universal qPCR mastermix (New England Biolabs Inc. ...
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bioRxiv - Biophysics 2023Quote: ... and added to 10 µl of 2xHiFi mix (New England Biolabs). We incubated the reaction at 50°C for 60 min ...
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bioRxiv - Synthetic Biology 2023Quote: ... Unannealed oligos were digested by adding 10 U ExoI (NEB, M0293S) to the reaction mix ...
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bioRxiv - Molecular Biology 2023Quote: ... and 25U T4 polynucleotide kinase (10 U/μL) (New England Biolabs). The radiolabelled RNA was purified by PAGE and resuspended in 40 μL nuclease-free water ...
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bioRxiv - Genomics 2023Quote: ... 10 µL of NEBNext Quick T4 DNA Ligase (New England Biolabs), 5 µL of Adapter mix AMX-F and 3 µL of nuclease-free water ...
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bioRxiv - Immunology 2023Quote: ... and 10 μL of PEG 8000 50% (NEB, cat. no: M0204S). The ligation mix was then incubated for at least 18 hours up to a maximum of 23 hours at 16 °C and then heat inactivated at 65 °C for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... and 10 μg/mL DNase I (New England Biolabs, Cat#M0303S) for continuous passages in HFFs ...
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Measuring carbohydrate recognition profile of lectins on live cells using liquid glycan array (LiGA)bioRxiv - Biochemistry 2023Quote: ... 10 μL of NEBuilder Hifi DNA assembly master mix (NEB #E2621S), and deionized H2O up to a total volume of 20 μL.
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bioRxiv - Bioengineering 2024Quote: ... 0.5 µL of 10× T4 DNA Ligase Buffer (NEB, Cat# B0202S), 0.25 µL of T4 DNA Ligase (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... freshly supplemented with 10 μl proteinase K (P8107S, New England Biolabs). Plates were stored slightly tilted to ensure the coverslip was completely covered with digestion solution and incubated at room temperature overnight ...
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bioRxiv - Microbiology 2024Quote: ... 10 mM ribonucleoside-vanadyl complex (New England Biolabs, Beverley, MA, USA), and 1× complete EDTA-free protease inhibitor (Roche ...
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bioRxiv - Microbiology 2020Quote: ... and a “bottom” strand with sequence 5’-AAAAC-(30bp spacer reverse complement)-3’ were phosphorylated with PNK (NEB, M0201S) in a 50 μL reaction volume (1.5 μL 100 uM top oligo ...
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bioRxiv - Microbiology 2020Quote: ... RNA and 3′ adapters were incubated at 22°C for 2.5 hr with 51 U of T4 RNA Ligase I (NEB) and 12 U of recombinant RNase inhibitor (Takara Bio ...
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bioRxiv - Genomics 2019Quote: 5’ repaired RNA was ligated to reverse 5’ RNA adaptor (5’-rCrCrUrUrGrGrCrArCrCrCrGrArGrArArUrUrCrCrA-3’) with T4 RNA ligase I (NEB) under manufacturer’s conditions for 2 h at 20°C ...
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bioRxiv - Neuroscience 2019Quote: Single nucleus RRBS were performed by first sorting PROX+/FOS+ and /FOS- neuronal nuclei in 96-well plates in 3 µL of 0.1× CutSmart buffer (New England Biolabs) per well as described in the Flow Cytometry section of the Materials and Methods ...
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bioRxiv - Molecular Biology 2022Quote: ... The 5’ end of the first block and the 3’ end of the last block contained SapI (NEB, R0569) recognition sites instead ...
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bioRxiv - Genetics 2022Quote: ... Pddx-23::ceDDX23::ddx-23 3’UTR (nEx2971 and nEx2972) was generated by HiFi DNA Assembly (New England Biolabs) of Pddx-23 ...
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bioRxiv - Molecular Biology 2020Quote: ... Selectively captured polyadenylated RNAs (1μg) were ligated directly to an DNA/RNA hybrid adapter (5’-CTACAC GACGCTrCrUrUrCrCrGrArUrCrUrNrNrN-3’) using T4 RNA ligase (NEB) at 37°C for 30 minutes ...
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bioRxiv - Biophysics 2019Quote: ... These cDNAs along with cDNA of hRIC-3-pHEMH19 were linearized with the restriction enzyme NheI (New England Biolabs). Subsequently ...
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bioRxiv - Genomics 2019Quote: ... was hybridized to the 3’ adapter and the 5’ adapter ligated by adding 2μL T4 RNA ligase buffer (NEB), 5μL PEG 8000 (NEB) ...
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bioRxiv - Bioengineering 2019Quote: ... The PCR product was inserted into pcDNA3.1 via 5’EcoRI/3’NotI restriction digestion and a standard ligation protocol (T4 DNA Ligase; New England BioLabs) to create pcDNA3.1-Ncadherin ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 µg of genomic DNA was incubated with 3 µg of in vitro transcribed sgRNAs and 3 µg of purified Cas9 protein in 20µl of 1x NEB3 buffer (New England Biolabs) at 37°C over night ...
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bioRxiv - Microbiology 2020Quote: pCrPV-3 and pCrPV-1A-DcDV-1A were linearized by digestion with BamHI-HF (New England Biolabs, Ipswich, Massachusetts). Wild-type and CrPV-DcDV RNA was prepared by in vitro transcription of 1 µg linearized plasmid using the mMESSAGE mMACHINE T7 transcription kit (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... unc-116::tagRFP::tom7 including unc-54 3’-UTR was PCR amplified using Phusion polymerase (NEB, Ipswich, MA, USA) from unc29p::unc-116::tagRFP::tom7 using following primers ...
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bioRxiv - Cell Biology 2021Quote: ... Par-3 PDZ1-APM and PDZ1-APMΔPDZ2 were first his-purified (described above) prior to incubation with amylose resin (NEB). Amylose-bound Par-3 was then washed and resuspended in binding buffer as described for GST proteins.
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bioRxiv - Cell Biology 2020Quote: PCR amplification of short fragments for screening purposes (<3 kbp) was conducted using OneTaq® (New England Bolabs (NEB), Ipswich ...
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bioRxiv - Biochemistry 2021Quote: ... Construct included 5’ and 3’ complementary overhangs for ligation into pPICZαA using NEBuilder HiFi DNA Assembly (New England Biolabs) to form pPICZαA_GH43_34 ...
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bioRxiv - Systems Biology 2021Quote: ... 3 μL of the reverse phasing primer pool (100 nM) and 15 μL of Q5 Mastermix (New England Biolabs). Cycle conditions were 4 minutes at 98°C followed by 20x (30 seconds at 98°C ...
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bioRxiv - Plant Biology 2021Quote: ... samples were incubated for 5 min on ice and 3 μL of 10X G5 buffer (New England BioLabs, UK) were added to the samples together with 1.5 μL of 25X concentrated protease inhibitor cocktail (Roche ...
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bioRxiv - Genomics 2022Quote: ... and water to make up 49 µl) for three hours at 37°C after which 3 µl NlaII (NEB) was added and the reaction incubated at 37°C for a further three hours ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified mIL-12bC197S-His6 in complex with mIL-12Rβ1D1-D2-His6 was subjected to an overnight Caspase-3 and EndoH (New England Biolabs) digest (1/100 w/w ...
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bioRxiv - Genomics 2022Quote: ... then A-tailed by incubating for 1 h at 37°C with 200 μM dATP and 0.2 U/μL Klenow fragment (3’-5’ exo-) in NEBuffer 2 (NEB), then Illumina PE adapter was added by incubating overnight at 20°C with 15 μM PE adapter and 2000 U T4 DNA ligase in ligase buffer ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 20 µL of denatured protein lysate was combined with 2.5 µL of 10X GlycoBuffer 3 (Cat.#B1720S; New England Biolabs) and 2.5 µL of Endo Hf in a total reaction volume of 25 µL ...
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bioRxiv - Plant Biology 2024Quote: ... This end-repaired DNA was subjected to A-tailing using the Klenow 3′–5′ exo− enzyme (New England Biolabs). The subsequent step involved the ligation of methylated adapters to the A-tailed DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... 1X Protoscript II buffer (50 mM Tris-HCl pH 8.3, 75 mM KCl, 3 mM MgCl2) 40 U Murine RNase Inhibitor (NEB), 5 mM DTT and pre-incubated for 10 minutes at 42 °C ...
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bioRxiv - Neuroscience 2023Quote: ... One piece of brain tissue (∼300 mg) was placed in 3 ml of ice-cold nuclei extraction buffer (NEB) [0.32 M sucrose ...
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bioRxiv - Genomics 2023Quote: The standard HiDEF-seq v2 protocol was followed with the exception of replacing Klenow fragment 3’→5’ exo-polymerase with one of the following: 9.6 U Bst large fragment (NEB), 9.6 U Bst 2.0 (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... The samples were then incubated at 42°C overnight with the addition of 3 μl of β-agarase (NEB). The DNA mix was then gently poured into combing reservoirs containing 1.2 ml MES and the genomic DNA was combed onto salinized coverslips (Genomic Vision ...
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bioRxiv - Developmental Biology 2023Quote: ... 3 μL of Round1 barcode mix (1x T4 DNA ligase buffer, 16 M/μL T4 DNA ligase (M0202L, NEB), 0.25 M/μL RNase Inhibitor ...