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Citations for New England Biolabs :
2051 - 2100 of 6344 citations for 6H Dibenzo b d pyran 3 pentanaminium 6a 7 10 10a tetrahydro 1 hydroxy N N N 6 6 9 hexamethyl 6aR trans 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... 3 μg of the final overexpression plasmids were linearized with either SmaI or AscI (New England Biolabs) and transformed consecutively into the DasKO strain as mentioned above ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA fragments were directly 3’-end dephosphorylated using 5 U of Antarctic Phosphatase (New England Biolabs, UK) for 30 minutes at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... The 3-4FnIII fragment and randomized 5FnIII domain were digested with BsmBI-v2 (New England Biolabs R0739S), purified by PCR cleanup kit (Fisher Scientific FERK0702) ...
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bioRxiv - Microbiology 2023Quote: ... All 3 fragments were amplified from pTRL2-His-GrgA 36 using Q5 DNA polymerase (New England Biolabs). Fragment 1 was generated using primers pgp3-pgp4-F and His-RBS-R (Table S1) ...
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bioRxiv - Developmental Biology 2022Quote: ... The bicistronic reporters used to detect regulatory elements within 3′ UTRs were constructed by Gibson Assembly (NEB) and standard methods ...
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bioRxiv - Molecular Biology 2022Quote: 3’ linker ligation (1x PNK buffer, 800 U T4 RNA ligase 2 truncated KQ (NEB, Cat#M0373L), 80 U RNaseOUT ...
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bioRxiv - Biochemistry 2024Quote: ... the concentrated protein at 3 mg/mL was incubated with c-AMP Protein Kinase A PKA (NEB) (molar ratio of 40:1 ...
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bioRxiv - Biochemistry 2024Quote: ... which was dephosphorylated at the 5’-end with 3 µl of Quick-CIP (5000 U/µl, NEB) in a total volume of 20 µl according to manufactureŕs instructions ...
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Atypical epigenetic and small RNA control of transposons in clonally reproducing Spirodela polyrhizabioRxiv - Plant Biology 2024Quote: ... were ligated to 3′ barcoded DNA adapters using truncated T4 RNA ligase 2 (New England Biolabs, #M0373). These fragments were separated in an 12% denaturing polyacrylamide-urea gel ...
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bioRxiv - Molecular Biology 2024Quote: ... crosslinked RNA fragments were dephosphorylated at their 3’ ends using T4 Polynucleotide Kinase (New England Biolabs, M0201S) and ligated to a pre-adenylated 3’ adapter (L3-App) ...
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bioRxiv - Microbiology 2024Quote: ... The 3’ ends of the VSV G and GP64 ORFs were PCR-amplified (using Taq polymerase, NEB) from DNA isolated from each fly line ...
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bioRxiv - Genomics 2024Quote: ... along with a 4.8uL 3:2 master mix of T4 ligase buffer:T4 ligase (New England Biosciences, NEB) and 9.4uL of nuclei buffer with BSA (NBB ...
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bioRxiv - Molecular Biology 2023Quote: For short read sequencing 3 μg yeast genomic DNA was digest with NEBNext dsDNA Fragmentase (NEB; # M0348S) in Fragmentase Reaction Buffer v2 at 30 °C for 30 min before stopping the reaction with EDTA ...
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bioRxiv - Cell Biology 2023Quote: ... crosslinked RNA fragments were dephosphorylated at their 3’ ends using T4 Polynucleotide Kinase (New England Biolabs, M0201S) and ligated to a pre-adenylated 3’ adapter (L3-App) ...
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bioRxiv - Molecular Biology 2023Quote: ... the 3’adapter was ligated to the dephosphorylated RNA using T4 RNA ligase 2 KQ (NEB, M0373L) at 25°C for 1h ...
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bioRxiv - Microbiology 2023Quote: ... was constructed by assembling 3 DNA fragments using the NEBuilder HiFi DNA assembly kit (New England Biolabs). All 3 fragments were amplified from pTRL2-His-GrgA (28 ...
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bioRxiv - Microbiology 2023Quote: ... All 3 fragments were amplified from pTRL2-His-GrgA (28) using Q5 DNA polymerase (New England Biolabs). Fragment 1 was generated using primers pgp3-pgp4-F and His-RBS-R (sTable 5) ...
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bioRxiv - Genetics 2023Quote: ... and A10 (Supplementary Table 3) were re-folded prior complexation with SpCas9 (Cas9 Nuclease, S. pyogenes; NEB), SpRY ...
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bioRxiv - Microbiology 2023Quote: ... CVO689-CVO586 (integrant 5’ end) and CVO321-CVO183 (integrant 3’ end) using Q5 Polymerase (New England Biolabs). PCRs were set up according to manufacturers’ instructions ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells expressing ATP5I-SNAP were stained with 3 µM SNAP-cell 647-SiR (Silicon rhodamine) (NEB) for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... was constructed by assembling 3 DNA fragments using the NEBuilder HiFi DNA assembly kit (New England Biolabs). All 3 fragments were amplified from pTRL2-His-GrgA 36 using Q5 DNA polymerase (New England Biolabs) ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 μM of each forward and reverse DNA oligos were annealed in T4 DNA Ligase buffer (NEB) by heating to 95° C for 5 mins then cooling at a rate of 4°C every 2 minutes until ambient temperature was reached ...
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bioRxiv - Cancer Biology 2024Quote: ... Exon 3 of Smad4 was amplified by PCR using the Q5 High Fidelity DNA polymerase (M0491S; NEB). Smad4 primers used were FWD ...
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bioRxiv - Cell Biology 2020Quote: ... and Illumina sequencing adaptor ligation (New England BioLabs, E6000B-10). Libraries were then indexed and PCR amplified (10 cycles ...
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bioRxiv - Genetics 2021Quote: ... and 10 U polynucleotide kinase (New England Biolabs, Beverly, MA) as instructed by the manufacturer ...
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bioRxiv - Genomics 2020Quote: ... 10 μL 10x T4 DNA Ligase Reaction Buffer (NEB B0202S), 0.5 μL 200x BSA (NEB ...
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bioRxiv - Genomics 2021Quote: Lambda exonuclease: 10 U λ exonuclease (New England Biolabs, M0262) in 60 μl 1×λ exonuclease reaction buffer was added and incubated at 37°C for 30 minutes.
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bioRxiv - Neuroscience 2019Quote: ... and 10 mM vanadyl-ribonucleoside complex (S1402S, New England BioLabs) with probes at final concentration of 1 ng/μl ...
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bioRxiv - Genomics 2019Quote: ... DpnII buffer and DpnII (10 U, New England Biolabs #R0543L) were added in a total volume of 50 µl and incubated at 37 °C for 1 h ...
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bioRxiv - Genetics 2021Quote: ... 10 uL of 10U/uL AluI restriction enzyme (NEB R0137S) was added to each sample ...
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bioRxiv - Genomics 2021Quote: ... 120 μL 10×NEB T4 DNA ligase buffer (NEB B0202), 100 μL of 10% Triton X-100 ...
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bioRxiv - Genetics 2020Quote: ... we added 10 µL of Shrimp Alkaline Phosphatase (NEB #M0371L) and incubated at 37°C for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... and 10 mM Ribonucleoside-Vanadyl Complex (NEB, Ipswich, MA, USA) on ice ...
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bioRxiv - Biochemistry 2020Quote: ... and 50 µL of 10 mM dNTPs (New England BioLabs), 100 µL 0.1 M DTT ...
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bioRxiv - Cell Biology 2021Quote: ... cells were initially incubated with SNAPcell block (10 µM; NEB) diluted in culture media for 20 min ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... 4 μl 10× T4 RNA ligase buffer (New England Biolabs), 4 μl T4 RNA ligase ...
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bioRxiv - Systems Biology 2020Quote: ... 22 μL of 10 mM/each of four dNTPs (NEB), 5.5 μL of TSO primer (100 μM) ...
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bioRxiv - Microbiology 2021Quote: ... coli (NEB 10-beta electrocompetent cells, New England BioLabs C3020K) and plated at a target bottleneck of 50,000 variants per duplicate library ...
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bioRxiv - Cell Biology 2022Quote: ... and 10 mM adenosine triphosphate (New England Biolabs, United States) was added to the 17 μl solution from the first step ...
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bioRxiv - Biochemistry 2022Quote: ... coli strain NEB 10-β competent cells (New England Biolabs) and plated on LB agar containing 50 μg/mL kanamycin ...
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bioRxiv - Genomics 2022Quote: ... The product was transformed into 10-beta Electrocompetent E.coli (NEB), by electroporation with 2.0kV ...
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bioRxiv - Microbiology 2022Quote: ... coli (NEB 10-beta electrocompetent cells, New England BioLabs C3020K) and plated at a target bottleneck of 100,000 variants per duplicate library ...
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bioRxiv - Molecular Biology 2020Quote: ... 5% DMSO and 10 units of λ exonuclease (NEB, M0262S) at 37°C for 30 min ...
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bioRxiv - Biochemistry 2021Quote: 10 ug of protein extract were digested with PNGaseF (NEB) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 µl of 2X Phusion Master Mix (NEB, Cat#M0531L), and 3.6 µl H2O (20 µl reaction) ...
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bioRxiv - Genomics 2022Quote: ... Each reaction contained 10 µL of Q5 Reaction Mix (NEB), 10 µL of Q5 GC Enhancer Buffer (NEB) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and 10 μl of 5 U/μl Klenow (NEB M0210)) and incubating 1h at 37ºC with rotation ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 2.5 μl of T4 PNK (10 U/μl, NEB M0201) and 0.5 μl of Klenow DNA polymerase (5 U/μl NEB M0210) ...
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bioRxiv - Microbiology 2022Quote: ... with the addition of 10 mg BSA (New England BioLabs). We added 0.1 femtomole 515F and 806R which do not have the barcodes and adapters to overcome initial primer binding inhibition because the majority of the primers do not match the template priming site ...
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bioRxiv - Microbiology 2022Quote: ... 2 μl 10× T4 RNA ligase reaction buffer (NEB, M0242), 10 μl 50% PEG 8000 (NEB ...