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Citations for New England Biolabs :
2251 - 2300 of 5258 citations for 6 Chlorotetrazolo 1 5 b pyridazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Transposed DNA was then purified on Diapure columns (Diagenode, Transposed purified DNA was then pre-amplified for 5 PCR Cycles using NEBNext High-Fidelity PCR MasterMix (NEB, M0541) and Illumina indexing primers ...
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bioRxiv - Developmental Biology 2023Quote: ... and 3.3 kb DNA sequence upstream of each respective gene was amplified using PCR and cloned into the vector containing the sequence gfp::rab-7::rab-7 3’UTR amplified from plasmid rgef-1p::gfp::rab-7::rab-7 3’UTR using PCR and primers 5’-atgagtaaaggagaagaacttttca-3’ and 3’-aagcttatcgataccgtcgac-5’ were used to generate tissue-specific gfp::rab-7::rab-7 3’UTR by using Gibson assembly (NEB E2611).
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bioRxiv - Developmental Biology 2023Quote: ... the pre- microRNA sequence of mir-51 was amplified from genomic DNA using PCR and primers 5’-cggcatcgacgacgacgacggtccgaaaagtccgtctacc-3’ and 3’- cagttggaattctacgaatgaactgtattgctgctgggc-5’ and the vector containing the sequence of rgef-1p and unc-54 3’UTR amplified from plasmid rgef-1p::aak-2::unc-54 3’UTR using PCR and primers 5’-cattcgtagaattccaactgagc-3’ and 3’-cgtcgtcgtcgtcgatgc-5’ were used to generate rgef-1p::mir-51::unc-54 3’UTR by using Gibson assembly (NEB E2611).
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bioRxiv - Developmental Biology 2023Quote: ... the tbb-2 3’UTR sequence was amplified from genomic DNA using PCR and primers 5’-tggatgaactatacaaatagatgcaagatcctttcaagca-3’ and 3’-aggttttcaccgtcatcacccgcgaaaaacccatgtaagt-5’ and the vector containing the sequence of pie-1p::his-15::gfp amplified from plasmid containing pie-1p::his-15::gfp::egg-6 3’UTR using PCR and primers 5’-ggtgatgacggtgaaaacct-3’ and 3’-ctatttgtatagttcatccatgcc-5’ were used to generate pie-1p::his-15::gfp::tbb-2 3’UTR by using Gibson assembly (NEB E2611). To add the wild type or mutant 3xmir-51 seed sequences ...
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bioRxiv - Developmental Biology 2023Quote: ... rgef-1p and gfp were inserted into a vector containing the sequence rab-7 amplified using PCR and primers 5’-atgtcgggaaccagaaagaa-3’ and 3’-aagcttatcgataccgtcgac-5’ to create rgef-1p::gfp::rab-7::rab-7 3’UTR using Gibson assembly (NEB E2611). A full list of reagents and resources can be found in table S2.
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bioRxiv - Genomics 2023Quote: ... Libraries were constructed using the NEBNext Ultra II directional RNA library preparation kit for Illumina according to the protocol for purified mRNA or ribosome-depleted RNA and with a 5-min RNA fragmentation step (NEB E7760). Library PCRs were supplemented with 2× SYBR dye (Sigma S9430 ...
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bioRxiv - Zoology 2024Quote: ... The annealed dsRNAs (2.5 µg) were checked on 1.5% agarose gel by running them together with 2 µL of dsRNA ladder (NEB# N0363S, Germany) (Fig ...
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bioRxiv - Biophysics 2024Quote: ... A 691 bp biotinylated DNA handle with a 29 nt 5′ overhang was amplified by Q5 DNA polymerase (NEB, Cat# M0491S) using a 5′ biotinylated forward primer and a reverse primer containing an abasic site 43 ...
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bioRxiv - Bioengineering 2024Quote: Samples were resuspended in 5 µl 1X NEBNext Cell Lysis Buffer of the NEBNext Single Cell/Low Input RNA Library Prep Kit (NEB, E6420). After 5 min incubation at RT ...
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bioRxiv - Plant Biology 2024Quote: RNA was isolated from leaves 4 and 5 using Trizol and cDNA was synthesized using MuMLV reverse transcriptase (New England Biolabs, Inc.) primed with random hexamers ...
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bioRxiv - Bioengineering 2024Quote: ... This strategy avoids 3’-terminal editing of the mismatched primers by the 3’-5’ exonuclease activity of Q5® High-Fidelity DNA Polymerase (NEB), increasing PCR specificity.61
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bioRxiv - Immunology 2024Quote: ... we performed an A-tailing of the PCR products of the YTS191-light chain cDNA by adding 5 µl of 10X ThermoPol Buffer (NEB, B9004), 10 µl of 10 mM ATP ...
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bioRxiv - Molecular Biology 2024Quote: ... The adaptor-ligated DNA on the magnetic beads was amplified by 5-8 cycles of PCR using NEBNext Ultra II Q5 Master Mix (New England Biolabs, M0544) and NEBNext Multiplex Oligos for Illumina (New England Biolabs) ...
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bioRxiv - Neuroscience 2024Quote: ... long amplification of the CHRFAM7A intron (exon A to exon 5) was carried out with the LongAmp® Taq DNA Polymerase Kit (ref. E5200S, New England Biolabs), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... was used to repair the sonicated DNA and successively 3’ A-tails were added by Klenow Fragment (3’→5’ exo-) (NEB, M0212S) and dATPs (NEB ...
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bioRxiv - Biochemistry 2024Quote: 27.4 μg of total RNA was fragmented into ∼150-200 nt fragments at 94 °C for 5 minutes using the magnesium RNA fragmentation buffer (NEB, E6186AVIAL), followed by purification with OCC ...
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bioRxiv - Biochemistry 2024Quote: The library was prepared following our previously reported procedure with slight changes.[21] 3′-End repair and 5′-phosphorylation were conducted with T4 polynucleotide kinase (PNK) (NEB, M0201S). 16 µL RNA was mixed with 2 µL 10× T4 PNK reaction buffer and 1 µL T4 PNK ...
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bioRxiv - Biochemistry 2024Quote: ... Precipitated RNAs were resuspended in Milli-Q water and labeled at the 5’-end with [γ-32P]-ATP using T4 polynucleotide kinase (New England Biolabs). Excess [γ-32P]-ATP was removed by G-25 MicroSpin column (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... The mixture was incubated for 3 days at 37C in the dark for conjugation and purified for 3 rounds using Monarch PCR & DNA Cleanup Kit (5 µg) (NEB, T1030S) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... and ligated to pre-adenylated linkers (NI-810 to NI-815) containing 5 nt sample barcodes unique for each sample using truncated T4 RNA ligase 2 (K227Q) (NEB; M0351L). Ligated fragments were separated from free linkers on a 15% polyacrylamide TBE-Urea gel and then pooled and purified for reverse transcription using RT primer NI-802 and ProtoScript II Reverse Transcriptase (NEB ...
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bioRxiv - Genomics 2023Quote: ... and incubated at 37°C for 1 hour with RNase A/T1 (Thermo, 1/20 volume) and RNase H (NEB, 1/50 volume). The DNA was then purified again.
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bioRxiv - Neuroscience 2021Quote: ... 1 μL cDNA was amplified using 1 μL Phusion High-Fidelity DNA Polymerase (NEB) in HF Phusion buffer with 10 μM primers ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μL Bacillus stearothermophilus (Bst) DNA polymerase (8 U μl-1) (New England Biolabs), 2 μL DNA template ...
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bioRxiv - Molecular Biology 2020Quote: ... amplicons were visualized on ~1% agarose gels with 1 kb plus DNA ladder (NEB) and SYBR Gold (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... and 1 μl of High Concentration T4 RNA Ligase 1 (New England BioLabs, M0437)] at 23°C for 5 h ...
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bioRxiv - Microbiology 2023Quote: ... lysates (1 mg) were incubated with MNase (10 units/1 mg lysate; NEB #M0247S) at 37°C for 60 min ...
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bioRxiv - Genetics 2023Quote: ... containing 1× Exonuclease I Buffer and 1 U/µL Exonuclease I (NEB, cat # B0293S), and incubated at 37 °C for 45 min ...
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bioRxiv - Microbiology 2024Quote: ... the product was incubated with 1 μl of dTTP (1 mM, NEB Catalog # N443S), 1 μL of Tdt buffer ...
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bioRxiv - Microbiology 2024Quote: ... the product was incubated with 1 μL of dTTP (1 mM, NEB Catalog # N)443S) ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1× CutSmart buffer (NEB) to 17 μl ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 x PNK buffer (NEB), and 20 U RiboLock ...
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bioRxiv - Genomics 2020Quote: ... 1 μl Exonuclease III (NEB), and NEBuffer 1 (NEB ...
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bioRxiv - Genomics 2019Quote: ... 1 ul Phi29 polymerase (NEB), 34 ul ultrapure water) ...
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bioRxiv - Genomics 2020Quote: ... RNase Inhibitor (1:40, NEB Murine RNAse Inhibitor or Thermofisher Ribolock ...
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Genetic mapping of fitness determinants across the malaria parasite Plasmodium falciparum life cyclebioRxiv - Evolutionary Biology 2019Quote: ... 1× BSA (New England Biolabs), 1 mM dNTPs (New England Biolabs) ...
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bioRxiv - Bioengineering 2019Quote: ... 1 × Zinc (supplied by NEB), 5 μL of 10 × GlycoBuffer 4 (supplied by NEB ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 μl Klenow exo-(NEB) and varying amounts of α[32P]-dCTP added before incubation for 1-3 hours at 37 °C ...
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bioRxiv - Synthetic Biology 2019Quote: ... 1× Taq polymerase buffer (NEB) and 2 μM or 20 μM each of the mutagenic nucleotide analogs 8-oxo-2′-deoxyguanosine-5′-triphosphate (8-oxo-dGTP ...
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bioRxiv - Genomics 2020Quote: ... 1 µL T4 PNK (NEB) and 6 µL H2O were mixed and incubated in a thermocycler (BioRad ...
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bioRxiv - Genetics 2019Quote: ... 1× T3 ligation buffer (NEB) and 300 ng total RNA with or without FTO treatment ...
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bioRxiv - Genomics 2021Quote: ... 1× BSA (New England Biolabs), 1 mM dNTPs (New England Biolabs) ...
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bioRxiv - Genomics 2021Quote: ... 1 μl of DpnI (NEB) was added to the reaction mixture to further incubate at 37 degrees for 10min ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and 1×Buffer 4 (NEB). The reaction was stopped (6 mM EGTA ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 1.875 U XRN-1 (NEB), 1 mM CaCl2 ...
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bioRxiv - Genomics 2020Quote: ... 1 µl RppH (5U, NEB)] was added ...
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bioRxiv - Genetics 2021Quote: ... 1 µl of T7EI (NEB) was then added to 19 µl of purified (QIAquick PCR Purification Kit ...
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bioRxiv - Genomics 2021Quote: ... 1 μL TET2 (NEB #7120S) and 1 μL T4-BGT (NEB #M0357S ...
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bioRxiv - Systems Biology 2020Quote: ... 1 μL T4 ligase (NEB), 2 μL T4 ligase buffer (NEB) ...
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bioRxiv - Biochemistry 2021Quote: ... and 1 mM ATP (NEB) in 1x PNK reaction buffer (70 mM Tris-HCl pH 7.6 ...
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bioRxiv - Cancer Biology 2020Quote: ... T7 Endonuclease 1 (NEB, M0302) was added to the mixture and incubated at 37°C for 15 minutes ...