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Citations for New England Biolabs :
2201 - 2250 of 10000+ citations for Cow WAP kazal immunoglobulin kunitz and NTR domain containing protein 2 WFIKKN2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... 2 pmol were digested to single nucleosides using the Nucleoside Digestion Mix (NEB # M0649), and the ratio of Ψ s versus uridines was determined via tandem quadrupole mass spectrometry (Supplementary Figure S2a).
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bioRxiv - Microbiology 2023Quote: ... 20-μL reactions contained 2 μM enzyme and 250 μM NTPs (New England BioLabs) in reaction buffer with 50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Developmental Biology 2023Quote: ... On-Bead 5’ Decapping reaction was done using NEBuffer 2 buffer (NEB, B7002S ®). Off-Bead Reverse Transcription followed the instructions in SuperScript ® IV Reverse transcriptase kit ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was ligated with 3′-adenylated adapters using T4 RNA Ligase 2 (NEB #MO351) for 4 hrs at 16°C ...
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bioRxiv - Microbiology 2023Quote: ... The annealed constructs were ligated using 2 µL T4 RNA ligase2 (NEB, 10U/µL), 3 µL 0.1% BSA (Ambion) ...
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bioRxiv - Molecular Biology 2023Quote: ... two pairs of oligonucleotides (Suppl. Table 2) were phosphorylated and ligated into BsbI (NEB) digested pDG459 plasmid ...
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bioRxiv - Molecular Biology 2023Quote: ... a pair of oligonucleotides (Suppl. Table 2) was phosphorylated and ligated into BsbI (NEB) digested pX459 and pX458 plasmids ...
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bioRxiv - Biophysics 2023Quote: ... 11 uL Exonuclease I Buffer and 2 uL Exonuclease I (New England Biolabs; M0293S) was added following reverse transcription and incubated at 37 °C for 15 min ...
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bioRxiv - Genomics 2023Quote: ... 20 of 2 U µL-1 Phusion High-Fidelity DNA Polymerase (New England Biolabs), 160 µL of 40 U µL-1 Taq DNA ligase (New England Biolabs ...
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bioRxiv - Genomics 2023Quote: ... Forked adapters (Supplementary Table S7) were annealed in NEBuffer 2 1X (NEB cat. B7002S) to a final concentration of 20 µM in a thermal cycler set to heat at 98°C and gradually cool down to 4°C with a 1°C per 20 sec gradient.
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bioRxiv - Genetics 2024Quote: ... the DNA-bound beads were digested with 2 μl BpmI (NEB, catalog number R0565L) for 30 min at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 μM of dsDNA was incubated with CpG methyltransferase (M. SssI, New England Biolabs) in the presence of 200 μM of S-adenosylmethionine ...
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bioRxiv - Immunology 2024Quote: ... washed in 10 mL RPMI+10% FBS + 2 µl RNase-free DNase I (NEB), and placed in single-cell suspension in 1 mL RPMI+10% FBS ...
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bioRxiv - Immunology 2024Quote: ... washed in 10 mL RPMI+10% FBS + 2 µl RNase-free DNase I (NEB), and stained with LIVE/DEAD Aqua for 15 minutes at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... Libraries were generated via PCR amplification using NEBNext HighFidelity 2× PCR Master Mix (NEB). The resultant libraries were purified again using the MinElute PCR Purification Kit (Qiagen) ...
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bioRxiv - Genomics 2024Quote: ... 0.4 µL RNase Inhibitor and 2 µL Induro RT (200 U/µL; NEB M0681S) were incubated for 15 minutes at 60°C ...
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bioRxiv - Cell Biology 2020Quote: Purified GST-Bbs5 protein (400 μg) was incubated with 6 μl of PKC (New England Biolabs) and 10 μl of ATP (New England Biolabs ...
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bioRxiv - Cell Biology 2020Quote: ... GST-fusion proteins on glutathione beads were pre-treated with 250 units CK2 (New England Biolabs) in protein kinase buffer (New England Biolabs ...
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bioRxiv - Developmental Biology 2021Quote: ... and the protease enzyme was further removed from hTNPO1 protein by Ni-NTA Magnetic Beads (NEB) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Precipitated proteins were wash once in acetone and resuspended in 1X blue loading buffer (NEB, B7703S).
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bioRxiv - Molecular Biology 2021Quote: ... The resulting PCR product was ligated into the pMal-c2x protein expression plasmid (New England Biolabs) adjacent to the malE gene to generate the pMAL-c2x-malE-L375-his10 plasmid encoding maltose binding protein (MBP ...
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bioRxiv - Developmental Biology 2021Quote: ... The guide RNA was injected into one-cell stage embryos together with Cas9 protein (N.E. Biolabs). Mutant animals were identified by PCR using the following primers ...
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bioRxiv - Biochemistry 2019Quote: Assays were conducted by incubating 0.1 and 1 μM SidJ in 1X Protein Kinase buffer (NEB), with 10 mM CaCl2 ...
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bioRxiv - Bioengineering 2021Quote: ... Amplified protein and pCTCON backbone were digested with BamHI and NheI restriction enzymes (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... 0.5 μM protein was incubated in the presence or absence of 20 μM SAM (NEB #B9003) in 25 mM HEPES pH 8.0 ...
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bioRxiv - Neuroscience 2021Quote: ... Surface biotinylated proteins immobilized on streptavidin agarose beads were denatured in Denaturing Buffer (New England Biolabs) at 100°C for 10 min in accordance with the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... All proteins were overexpressed in Escherichia coli BL21 Codon-Plus competent cells (C2527, New England Biolabs) and grown at 37 °C in 2 % Luria Broth containing 30 mg/L kanamycin until OD600 reached 0.6 ...
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bioRxiv - Microbiology 2021Quote: One microgram of full-length S protein was treated with 1 μg of FXa (P8010L, NEB), furin (P8077S ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 mM MnCl2 only or together with 1 μl Lambda Protein Phosphatase (New England Biolabs). After incubation at 30°C for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... MBP-tagged protein was captured by gravity flow affinity chromatography using amylose resin (New England Biolabs). Captured protein was washed with wash buffer (50mM Tris/HCl pH 7.6 ...
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bioRxiv - Biochemistry 2022Quote: ... The fusion protein was purified from the supernatant using amylose resin (New England Biolabs, Ipswich, MA), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... together with the Cas9 protein (EnGen® Cas9 NLS, S. pyogenes, M0646M, NEB, Ipswich, MA, USA) was injected into zebrafish embryos at one-cell stage ...
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bioRxiv - Molecular Biology 2020Quote: ... half of the samples were treated with a broad-spectrum lambda protein phosphatase (NEB Cat#P0753L) and PNGase-F (Promega Cat#V4831 ...
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bioRxiv - Microbiology 2019Quote: Cellular extracts were incubated with 2,000 units of λ-protein phosphatase (λ-Ppase) (New England Biolabs) in 50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein-RNA complexes were radiolabeled on-bead in 40 µl reactions with T4 polynucleotide kinase (NEB) and 2 µl [γ-32P]ATP (6,000 Ci/mmol ...
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bioRxiv - Genomics 2019Quote: Cas9/RNP complexes were assembled with 25 pmol Cas9 protein (EnGen Cas9 NLS, New England Biolabs) and 50 pmol gRNA just prior to electroporation ...
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bioRxiv - Cell Biology 2019Quote: ... SNAP-FOR1(3P,FH2) protein was labeled with SNAP-549 dye (New England Biolabs, Ipswich, MA) as per manufacturer’s instructions prior to each TIRF experiment.
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bioRxiv - Cell Biology 2020Quote: Total protein extracts were obtained from cell pellets directly resuspended in the loading buffer (Biolabs B7709S) containing 1% SDS ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were prediluted to a concentration of 750 nM in 1x CutSmart buffer (New England Biolabs) and preloaded with guide DNA or guide RNA in a 1:1 ratio at 37°C for 15 min ...
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bioRxiv - Neuroscience 2020Quote: ... All proteins were overexpressed in Escherichia coli BL21 Codon-Plus competent cells (C2527, New England Biolabs) and grown at 37 °C in 2 % Luria Broth containing 30 mg/L kanamycin until OD600 reached 0.6 ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos were injected with 200-300 pg gRNAs and 1.6 ng EnGen Cas9 protein (#M0646M NEB). The target and gRNA sequences ...
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bioRxiv - Molecular Biology 2021Quote: ... The antibodies were captured using 50 µl of protein G magnetic beads (S1430, New England Biolabs) incubated for 60 min with end over end mixing at 4°C ...
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bioRxiv - Microbiology 2022Quote: All recombinant proteins were expressed in Escherichia coli T7 Express lysY/Iq cells (New England Biolabs). Except for GST-PP1γ ...
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bioRxiv - Biochemistry 2022Quote: ... CBP (Chitin Binding Protein, produced by N. Martín in Dr. Casanova’s lab, New England Biolabs Protocol) was used as a secondary antibody at 1:300 to detect chitin and visualize the tracheal branches ...
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bioRxiv - Biochemistry 2022Quote: ... with recombinant proteins (200-500 ng) and yeast purified CK2 or recombinant human CK2 (NEB, P6010S). Proteins were resolved by SDS-PAGE and the gels were exposed to autoradiography or stained with Coomassie blue.
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bioRxiv - Microbiology 2022Quote: ... individual sgRNAs and Cas9 protein in 1X NEB 3.1 buffer (New England Biolabs, Ipswich, MA, USA) were incubated at 37°C for 2 h ...
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bioRxiv - Microbiology 2022Quote: ... The DST was removed by incubating the protein with 64 units of Enterokinase light chain (BioLabs) in 10 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2023Quote: Genetically modified zebrafish lines were created using Cas9 protein (EnGen® Spy Cas9 NLS, #M0646T, NEB) and previously established protocols combining PCR and reverse transcription to generate guide RNAs44 ...
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bioRxiv - Microbiology 2022Quote: ... The EcRecA protein was purified in a similar manner and then compared to commercial EcRecA (NEB). The protein activity of commercial NEB and purified proteins was equivalent ...
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bioRxiv - Biochemistry 2024Quote: ... crude extracts were treated with the λ-protein phosphatase (λ-PPase; New England BioLabs, Cat# P0753S) before application on the gel ...