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Citations for New England Biolabs :
2001 - 2050 of 10000+ citations for Cow WAP kazal immunoglobulin kunitz and NTR domain containing protein 2 WFIKKN2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... To make the sgRNA we linearized purified pDR274 containing the guide sequence with Dra1 and used a T7 RNA polymerase for in vitro transcription (NEB). The pMLM3613 plasmid encoding cas9 was used for in vitro transcription using the SP6 mMessage mMachine Kit (Ambion ...
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bioRxiv - Synthetic Biology 2020Quote: 3’-dT-tailed barcode cassettes were ligated into a 3’-dA-tailed vector and the DNA circularized using T4 DNA Ligase in a PEG-6000 containing buffer (Quick Ligation Buffer, NEB). Ligation was performed at a 30:1 insert:vector molar ratio at bench temperature (24°C ...
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bioRxiv - Molecular Biology 2019Quote: ... the Bbs1 cutting site containing guide RNA sequences were annealed and then cloned into the guide RNA vector using T4 ligase (New England Biolabs). The plasmids were transfected with Lipofectamine 3000 in HeLa cells ...
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bioRxiv - Molecular Biology 2020Quote: ... and inserting a synthetic gBlock (IDT) containing an FRT5 site followed by an in-frame stop codon via NEB HiFi Assembly (NEB) to create pTaDaG2-FRT5 ...
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bioRxiv - Biophysics 2021Quote: ... and 15–25 μM BG-oligonuculeotides were labeled with ∼1 μM myosin II containing a C-terminal SNAP-tag (NEB) in anion-exchange elution buffer for 30 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... the N gene was amplified from the BAC containing viral cDNA with a high fidelity polymerase (Taq Q5 Hot Start High-Fidelity 2x Master Mix, Ref. M0494S, NEB) and primers PV012-F (5’-ACT-GTA-ATA-CGA-CTC-ACT-ATA-GGG-ATG-TCT-GAT-AAT-GGA-CCC-CAA-AAT-C-3’ ...
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bioRxiv - Bioengineering 2021Quote: ... 0.1 μl of each PCR reaction was amplified with index-containing primers to reconstitute the TruSeq adaptors using the Q5 High-Fidelity DNA Polymerase (New England Biolabs): (98 °C ...
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bioRxiv - Biophysics 2021Quote: ... Two experiments were conducted in which the vacuum filling of the channels with buffer was followed by a heating/diffusion step of 1 hour at 40°C with the reservoir filled with a solution containing both DNase I (at 0.096U/μl) and BSA (NEB B9000S) at 0.13mg/ml or 0.40mg/ml ...
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bioRxiv - Genomics 2021Quote: ... two complementary oligos containing the guide sequence and a BbsI recognition site were annealed and ligated with the BbsI (New England Biolabs) digested target plasmid ...
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bioRxiv - Genomics 2019Quote: ... the immunoprecipitated protein-bound sepharose beads was washed four times with PBS and suspended in the DNaseI digestion buffer containing DNaseI (6 Units of M0303S NEB). The samples were incubated for 10 minutes at room temperature and centrifuged at low speed to separate two fractions ...
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bioRxiv - Genomics 2020Quote: ... and half of the DNA was 3’-end labeled for 1 h at 37 °C in a 10-μl reaction containing 6 units of terminal deoxynucleotidyl transferase (New England Biolabs), 0.25 mM CoCl2 ...
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bioRxiv - Biochemistry 2019Quote: ... The digested PCR product was circularized by T4 DNA ligase in a total volume of 500 μL 1x T4 DNA Ligase Reaction Buffer containing 100 μL digested PCR product and 10 kU T4 DNA Ligase (NEB). The reaction was incubated overnight at 16°C and purified by ethanol precipitation ...
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bioRxiv - Genetics 2020Quote: ... pDR274 vector [Addgene plasmid 42250] for in vitro gRNA production containing a T7 promoter upstream of gRNA scaffold sequence was digested with BsaI enzyme (NEB). It was then used as a backbone for cloning the annealed oligonucleotides (dpy-10T ...
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bioRxiv - Physiology 2021Quote: ... and the Esp3I fragment of the pPVxRF3 vector (a gift from S. Kondo) containing Venus and 3xP3-dsRed-Express2 using NEBuilder HiFi DNA Assembly Master Mix (NEB). The combined fragment was cloned into pBluescript ...
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bioRxiv - Biochemistry 2021Quote: ... vector were constructed by digesting pMS329 and pWZ414-F12 respectively with ClaI/AgeI and with BlpI/BamHI to remove a segment of their H3 genes followed by recombination with synthetic DNA fragments (Twist Bioscience) containing the R49C mutation by the Gibson assembly protocol (New England Biolabs). Similarly ...
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bioRxiv - Biochemistry 2021Quote: The α-(1,2)-Galf-containing N-linked glycans were released from 1 mg Transglucosidase L “Amano” (Amano) by using PNGase F (New England Biolabs) under denaturing conditions ...
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bioRxiv - Biochemistry 2021Quote: ... containing the cDNA of interest were cloned into pMXS-IRES-Blast linearized with BamHI and NotI by Gibson Assembly (NEB). cDNA vectors along with retroviral packaging vectors gag-pol and CMV VSV-G were transfected into HEK-293T cells ...
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bioRxiv - Microbiology 2021Quote: ... or used as a template to generate chimeric fimbrial gene clusters containing different G adhesins using the T4 DNA ligase (NEB). PCR fragments containing G adhesin genes were obtained using primer pairs CMD2171_F and CMD2172_R (for papG class I from strain J96) ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were incubated at 37ºC in complete growth medium containing either 50 nM SNAP-tetramethylrhodamine (TMR) star (New England BioLabs: S9105S) for 60 min ...
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bioRxiv - Biochemistry 2021Quote: ... 50 μl of lysed cells were aliquoted to 8 tubes containing 450 μl of digestion mix (1× NEB 1 buffer, 1% triton X-100, and 100 U HpaII enzyme [NEB]) and incubated at 37 °C for 3 hours with constant shaking ...
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bioRxiv - Developmental Biology 2020Quote: ... and specific primers containing BstbI and XhoI restriction enzyme sites were used with Q5 high-fidelity DNA polymerase (New England Biolabs). The resulting amplicons were cut using BstbI and XhoI ...
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bioRxiv - Developmental Biology 2021Quote: A ~5kb genomic sequence containing 2kb promoter and entire genomic region of tes-1 was PCR amplified using Phusion polymerase (NEB). The primers used were ...
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bioRxiv - Cell Biology 2020Quote: 50 μl of lysed cells were aliquoted to 8 tubes containing 450 μl of digestion mix (1x NEB 1 buffer, 1% triton X-100, and 100 U HpaII enzyme (NEB)) and incubated at 37°C for 3 hours with constant shaking ...
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bioRxiv - Genetics 2021Quote: ... The intact pCFD6 vector was used as a template for the production of the three overlapping gRNA-containing inserts by PCR with Phusion Flash polymerase (NEB) using pairs of primers (pCFD6-Dro5A ...
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bioRxiv - Microbiology 2021Quote: ... This allowed cassettes (gBlock Gene Fragments from IDT) containing various copyback genomes to be ligated using Gibson Assembly (NEBuilder HiFi DNA assembly, NEB). The deletion genome core plasmid was designed to contain Eco RV and Stu I restriction sites in the trailer and leader (GP ...
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bioRxiv - Microbiology 2021Quote: ... We then used a 1690 bp synthetic fragment (gBlock Gene Fragments from IDT) containing the digested L gene sequences and EGFP to be inserted by Gibson Assembly (NEBuilder HiFi DNA assembly, NEB). The gBlock contained mutations to replace nucleotides CT at rCDVRIVenus(6 ...
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bioRxiv - Microbiology 2020Quote: ... The reaction mixture was then dried completely and resuspended in a mixture containing 50 mM ammonium bicarbonate and PNGase F (New England Biolabs) using only H2O18 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... was linearized in a 500 μL reaction volume containing 100 units of ApaLI enzyme for 4-6 hours at 37°C in CutSmart buffer (New England Biolabs). The DNA was then ethanol precipitated as previously described (76) ...
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bioRxiv - Microbiology 2020Quote: ... The Vag8 quadruple mutant (H209A Y234A E237A W285A) was produced by Gibson Assembly of overlapping fragments containing the desired mutations using NEBuilder HiFi Master Mix (NEB). Purification of Vag8 mutants was done as described above for wild type Vag8.
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bioRxiv - Immunology 2020Quote: ... Single antigen specific memory B cells were sorted on BD FACS Aria II into 96-well PCR plates (Axygen) containing 10 μl per well of lysis buffer (10 mM DPBS, 4 U Mouse RNase Inhibitor, NEB). Plates were immediately frozen on dry ice and stored at 80 C or processed for cDNA synthesis.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were labelled by replacing complete growth medium with 500 μl serum free DMEM/F12 containing 200 nM SNAP Surface Alexa Fluor-488 (New England Biolabs) and were incubated for 30 min at 37°C with 5% CO2 ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was then transferred to a fresh low-bind tube containing pre-washed 5 µl amylose magnetic beads (NEB) and rotated for 45 min at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... PDGFRβ DNA inserts containing the desired mutations were generated by PCR amplification using the Phusion High-Fidelity DNA Polymerase (New England Biolabs) and subcloned into pLenti CMV Hygro DEST for cellular studies ...
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bioRxiv - Biochemistry 2022Quote: ... KIT DNA inserts containing the desired mutations were generated by PCR amplification using the Phusion High-Fidelity DNA Polymerase (New England Biolabs). The inserts were subcloned into either pFastBac 1 vector for structural studies or pBABE-puro vector for cell-based studies ...
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bioRxiv - Immunology 2022Quote: ... Beads were washed and resuspended in NEBuffer 3 containing Calf Intestinal Alkaline Phosphatase at a concentration of 0.5 U/μl (NEB, M0290) to dephosphorylate the RNA ...
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bioRxiv - Molecular Biology 2023Quote: ... containing an N-terminal 6xHis tag and TEV site was produced in Escherichia coli NiCo21 (DE3) cells (New England Biolabs) as previously described in https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6069193/ ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplified yciF (with Strep II tag on C-terminal) and pQE60 plasmid (containing an IPTG inducible T5 promoter) were digested with BamHI and EcoRI (New England Biolabs) and purified by gel extraction (MinElute gel extraction kit ...
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bioRxiv - Evolutionary Biology 2022Quote: ... melanogaster strain to be injected and inserted into plasmids containing fluorescent eye markers using NEBuilder Hi-Fi DNA assembly (NEB). See key resources table for primers sequences and donor plasmids.
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bioRxiv - Microbiology 2022Quote: ... which was assembled with oligo DNA containing Myc or FLAG tag sequence using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs). The resultant plasmids were named pcDNA3-C-Myc or pcDNA3-C-FLAG ...
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bioRxiv - Neuroscience 2022Quote: ... was linearized for 18 hours at 37°C in a 250 μl reaction containing restriction enzyme NheI and CutSmart buffer (New England Biolabs). The linear DNA plasmid was next used to produce complementary RNA (cRNA ...
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bioRxiv - Microbiology 2022Quote: ... and Atd1 and containing an N-terminal TEV cleavage site were cloned directly via Gibson assembly (NEBuilder HiFi, New England Biolabs) into BamHI/NotI-digested pGEX4-1 (GE Healthcare) ...
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bioRxiv - Biochemistry 2024Quote: ... and tRNAGln with 5 nt-long 5’ leader and same 24 nt-long 3’ trailer as tRNATyr (5–tRNAGln–24) – were transcribed in reactions containing 1x T7 RNA polymerase reaction buffer (NEB), 0.001% (w/v ...
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bioRxiv - Cell Biology 2024Quote: ... The amplicons were digested with SacI and EagI ligated into the plasmid containing the ATG8 promoter linearized with HindIII (New England Biolabs) and SalI (New England Biolabs) ...
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bioRxiv - Microbiology 2024Quote: ... The coverslips are then incubated for 30 min at 37°C with DMEM supplemented with 10% SVF containing 0.12 μM SNAP-Cell TMR-Star (New England Biolabs #S9105S), rinsed twice with PBS and incubated for 45 min with Dapi (1μg/ml) ...
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bioRxiv - Genetics 2024Quote: ... DNA was isolated from HepG2 cells and the regions were amplified with PCR primers containing restriction enzyme (RE) cutting sites for NsiI (New England Biolabs, NEB) and BamHI/HindIII (NEB ...
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bioRxiv - Immunology 2024Quote: Reverse-transcription on total RNA (100 to 500 ng) was performed with random hexamer/oligo-dT-Primer containing LunaScript RT Supermix (New England Biolabs) following the manufactureŕs instructions ...
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bioRxiv - Immunology 2024Quote: ... Two microliters were subsequently used in real-time quantitative PCR reactions containing SYBR-green based Luna universal dye qPCR mix (New England Biolabs) and gene specific PCR primers ...
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bioRxiv - Cancer Biology 2024Quote: ... Sequencing libraries were generated by amplifying sgRNA sequences from genomic DNA using bar-coded Illumina-compatible adapter-containing primers and NEBNext High-Fidelity 2x PCR Master Mix (New England BioLabs). PCR products were pooled and purified with a ZymoSpin V column with Reservoir (Zymo Research) ...
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bioRxiv - Biochemistry 2023Quote: ... The pCR8[mZC3H18] plasmid was used as a template to generate subsequent ZC3H18 cDNA constructs that were cloned into a piggyBAC (pB) vector containing an N-terminal MYC tag and BSD selection marker using NEBbuilder HiFI DNA assembly (NEB). OsTIR1-HA ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli cells and streaked onto Luria-Burtani (LB) plates containing carbenicillin. All-by-all repressor constructs (Fig. 5c) were cloned by digestion with BsiWI-HF (NEB) and BbsI (NEB) ...