Labshake search
Citations for New England Biolabs :
2101 - 2150 of 6017 citations for Type 1 Angiotensin II Receptor AGTR1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The DNA from different barcoded samples was pooled and the adapter AMII (ONT) was ligated using the NEBNext Ultra II Ligation Module (NEB).
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Chromatin priming elements direct tissue-specific gene activity prior to hematopoietic specificationbioRxiv - Genomics 2023Quote: ... RNA-Seq libraries were then prepared using the NEBNext® Ultra ™ II Directional RNA Library Prep Kit (NEB, E7760) total RNA with rRNA depletion protocol using 100 ng of input RNA ...
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bioRxiv - Genomics 2023Quote: ... we used NEBNext FFPE DNA Repair and Ultra II End Repair Module reagents (New England Biolabs Inc., Ipswich, United States) and incubated at 20°C for 5 minutes and then at 65°C for 5 minutes ...
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bioRxiv - Immunology 2023Quote: ... Polyadenylated transcript enrichment and strand specific library preparation was completed using a NEBNext Ultra II mRNA kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: The cellular mRNA from THP-1 cells was reverse transcribed with oligo-dT primer through the Protoscript II First Strand cDNA Synthesis Kit (New England Biolabs) after TRIzol (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... standard curve transcripts and viral RNA in the samples were reverse transcribed with a reverse E1 primer containing a nongenomic tag sequence74 5’-CAGACAGCACTCGTTCGTACAC-3’ through the Protoscript II First Strand cDNA Synthesis Kit (New England Biolabs). The (+ ...
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bioRxiv - Genomics 2023Quote: ... RNA sequencing libraries were prepared by NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB catalogue number E7760S). Libraries were sequenced as 150 bp paired-end reads using a Novaseq 6000 ...
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bioRxiv - Neuroscience 2023Quote: ... mRNA was eluted from the oligo-dT beads and fragmented by incubating with the First Strand Synthesis Reaction buffer and Random Primer Mix (2×) from the NEBNext Ultra II Directional Library Prep Kit for Illumina (#NEBE7760; New England Biolabs) for 15Lmin at 94°C ...
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bioRxiv - Genomics 2023Quote: Directional poly(A)+ RNA-Seq libraries were prepared using 300 ng of DNase-treated RNA using the Poly (A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep kit for Illumina (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA libraries were generated using 1µg RNA with the magnetic mRNA isolation module and NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs). DNA library was amplified by PCR and quality was analyzed using the fragment analyzer (Advanced Analytical) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... RNA library preparation was performed on total or polysomal RNA using a NEBNext Poly(A) mRNA Magnetic Isolation Module and a NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) according to the manufacturer’s instructions with 1 μg of RNA as a starting point ...
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bioRxiv - Cell Biology 2022Quote: ... Library construction: The RNA sequencing library was constructed using NEBNext® Ultra II RNA Library Prep Kit (New England Biolabs) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2022Quote: ... Non-directional sequencing libraries were completed with the “NEBNext Ultra II FS DNA Library Prep Kit for Illumina” (NEB #E7805) and subsequently analyzed on an Illumina NextSeq 550 with v2.5 reagent kits following manufacturer’s protocols.
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bioRxiv - Cancer Biology 2023Quote: ... Purified DNA was submitted to NGS library preparation using NEBNext Ultra II DNA Library Prep Kit (New England BioLabs, E765) and sequenced with NovaSeq 6000 (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: ... Eluted DNA was used for qPCR or sequencing library preparation with NEBNext Ultra II DNA Library Prep Kit (NEB; E7645) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2022Quote: ... and sgRNA cassettes were amplified using 22 cycles of PCR using NEBNext Ultra II Q5 PCR MasterMix (New England Biolabs). Sequencing was performed on a NovaSeq 6000 (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... and CUT&RUN samples from two replicate (two independent CRISPRi clones) using NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645, NEB). The manufacture’s protocol was employed with the following changes ...
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bioRxiv - Cancer Biology 2023Quote: ... The ligation product was amplified through 10 reaction cycles to generate a whole genome library (MC library) using NEBNext Ultra II DNA library Prep Kit for Illumina (New England Biolabs) and 750ng MC library was used for exome enrichment ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 ng DNA was subjected to library preparation using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... continued with end-repair and adaptor ligation by following the NEBNext® Ultra™ II DNA Library Prep Kit (NEB). Ligated DNA was affinity-purified with Dynabeads MyOne Streptavidin C1 beads (Invitrogen ...
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bioRxiv - Genomics 2022Quote: ... Extracted RNA was prepared for strand specific sequencing by the Biomedical Research Centre Sequencing Core (UBC, Vancouver) using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina and polyA selection (NEB). Libraries were sequenced on an Illumina Miseq machine using 300 cycles ...
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bioRxiv - Genetics 2023Quote: ... Libraries were then generated using 10 ng of DNA with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB). The quality of the libraries was assessed with Agilent 2100 Bioanalyzer Instrument ...
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bioRxiv - Cell Biology 2023Quote: ... Subsequent steps of RNA-seq were outsourced to Azenta who generated sequencing libraries from polyA-enriched RNA (captured with oligo-dT beads) using the NEBNext Ultra II RNA library prep kit for Illumina (NEB), multiplexed them ...
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bioRxiv - Developmental Biology 2023Quote: ... Libraries were constructed from ∼2ng DNA and prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB). Library quality was assessed by Fragment Analyzer NGS ...
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bioRxiv - Developmental Biology 2023Quote: ... Library preparation for high-throughput sequencing was performed on 100 ng of rRNA depleted RNA using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Sequencing was done with a NextSeq 500/550 High Output Kit v2.5 (150 Cycles) ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA-seq libraries were constructed by NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (NEB #E7775). Briefly ...
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bioRxiv - Genomics 2023Quote: ... was used to prepare libraries with the NEBNext Ultra II library preparation kit with unique dual index primers (New England Biolabs). The library quality and quantity were verified by BioAnalyzer DNA 1000 (Agilent ...
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bioRxiv - Genomics 2022Quote: ... Three hundred ng of mRT-PCR products from each of two reactions per genome were pooled for the treatment with NEBNext Ultra II End repair and dA-tailing Module (New England Biolabs). According to the manufacturer’s protocols ...
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bioRxiv - Genetics 2023Quote: ... RNA was prepared for sequencing using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760). Sequencing was performed on an Ilumina NovaSeq 6000 instrument equipped with an S4 flow cell generating 150bp paired-end reads ...
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bioRxiv - Biochemistry 2023Quote: ... 5′ phosphorylated and 3’ A-tailed by NEBNext Ultra II End Prep Enzyme Mix following the manufacturer’s instruction (New England Biolabs #E7645L). An adaptor was ligated ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760S/L) following the kit protocol ...
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bioRxiv - Microbiology 2023Quote: ... 50 ng of total RNA was used as input into the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs). Libraries were prepared following the manufacturer’s protocol for use of the kit with purified mRNA or rRNA-depleted RNA ...
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bioRxiv - Genomics 2023Quote: ... were PCR-amplified (2 PCR reactions, 12 cycles) using Illumina adapter-specific primers and NEBNext Ultra II Q5 Master Mix (NEB). After library profile analysis by Agilent 2100 Bioanalyser (Agilent Technologies ...
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bioRxiv - Genomics 2023Quote: ... The fragmentation pattern was assessed via agarose gel electrophoresis and 0.5-1 μg of fragmented gDNA was used to prepare sequencing libraries using the NEBNext Ultra II DNA Library Preparation Kit (New England Biolabs), according to manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... The remaining steps of library preparation were done using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs). Adapters and PCR primers were purchased from New England BioLabs.
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bioRxiv - Plant Biology 2023Quote: ... The library was prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, #E7760) and amplified using NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs Set 2 ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA libraries were generated from 500 ng of total RNA using the NEBnext Ultra II RNA Library Prep kit (New England Biolabs), following the manufacturer’s protocols ...
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bioRxiv - Immunology 2023Quote: ... RNAseq libraries were constructed by the Transcriptional Regulation and Expression (TREx) Facility at Cornell University using the NEBNext Ultra II RNA Library Prep Kit (New England Biolabs).
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bioRxiv - Microbiology 2023Quote: ... DNA removal coupled with cDNA synthesis were conducted using NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s recommended protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We then quantified DNA concentrations using a Qubit fluorometer and submitted 100 ng DNA to Genome Quebec where technicians prepared shotgun DNA libraries using the NEB Ultra II (New England Biolabs) library preparation kit and then sequenced the libraries using four NovaSeq6000 S4 PE150 lanes.
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bioRxiv - Pathology 2023Quote: ... the library preparation process was started from the “End-prep” step of the aforementioned protocol using NEBNext Ultra II End repair/dA-tailing Module (New England Biolabs,NEB). A Blunt/TA Ligase Master Mix (NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... The remaining steps of library preparation were done using and the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs). Adapters and PCR primers were purchased from New England BioLabs.
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bioRxiv - Biochemistry 2023Quote: ... the pool was subjected to NEBNext FFPE DNA Repair and Ultra II End-prep kits (M6630 and E7546, New England Biolabs). After that ...
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bioRxiv - Developmental Biology 2023Quote: ... Then the sequencing library was generated by the NEBNext® Ultra II DNA Library Prep Kit (New England Biolabs; E7103S) and NEBNext® Multiplex Oligos for Illumina® (New England Biolabs Index Primers Set 1 ...
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bioRxiv - Microbiology 2023Quote: ... All samples were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs, #E7760L), with an initial amount of 100 ng total RNA in 12 μl ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was obtained by reverse transcription of 0.5 μg of total RNA using ProtoScript II First Strand cDNA Synthesis Kit (NEB, #E6560S). Three independent biological replicates were prepared for each genotype ...
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bioRxiv - Microbiology 2023Quote: ... The amount of RNA template was equalized for reverse transcription using the Protoscript II First Strand cDNA Synthesis Kit (New England Biolabs) and random hexamers ...
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bioRxiv - Microbiology 2023Quote: ... Library preparation was conducted by the standard ≥100 ng protocol from the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB) with minor modifications ...
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bioRxiv - Immunology 2023Quote: ... Stranded RNA sequencing libraries were prepared as described using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (Cat# E7760L; New England Biolabs). Purified libraries were qualified on an 4150 TapeStation (Agilent Technologies ...
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bioRxiv - Plant Biology 2023Quote: ... followed by first-strand cDNA synthesis with the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs) and NEBNext Multiplex Oligo for Illumina (New England Biolabs ...