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Citations for New England Biolabs :
2101 - 2150 of 2459 citations for 6 CHLORO 3 METHYLPYRIDO 3 4 D PYRIMIDIN 4 3H ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... sgRNAs were amplified using a one-step barcoding PCR using NEBNext High Fidelity 2X PCR Master Mix (NEB, M0541L) and the following primers:
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bioRxiv - Synthetic Biology 2021Quote: ... Table S2.1) for hydrogel functionalisation were synthesised by mixing one volume of 40 mM BG-PEG-NH2 (NEB S9150S) or 40 mM chloroalkane-PEG-NH2 (Promega P6741 ...
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bioRxiv - Microbiology 2020Quote: ... RdRP_SARSr-F2 (GTGARATGGTCATGTGTGGCGG) and dRP_SARSr-R1(CARATGTTAAASACACTATTAGCATA) using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) and a CFX96 Real-Time System ...
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bioRxiv - Microbiology 2021Quote: ... Or reverse transcription and qRT-PCR were conducted in one step using 0.1 μg RNA as template with the Luna Universal Probe One-Step RT-qPCR kit (New England Biolabs) following manufacturer’s protocols ...
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bioRxiv - Biochemistry 2022Quote: ... The lysed cells were directly subjected to qRT-PCR using Luna Universal One-Step RT-qPCR Kit (NEB, #E3005L). The primer sets used in qRT-PCR to detect CI-MPR and CD-MPR mRNA are 5’-CATTCAGTGGGTGACTCTGTT-3’/5’-TGCTCTGGACTCTGTGATTTG-3’ and 5’-GCTCTAGTGAAGAGGCTGAAAC-3’/ 5’-GCACACCCTGAAGATGTAGATG-3’ ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR was performed using Luna® Universal Probe One-Step RT-qPCR kit (E3006L; New England Biolabs (NEB), Ipswich ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR was performed using Luna® Universal Probe One-Step RT-qPCR kit (E3006L; New England Biolabs (NEB), Ipswich ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Quantification of viral genomes was performed using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) with a primer/probeset binding in the orf1ab region (forward ...
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bioRxiv - Cancer Biology 2024Quote: ... One genotyping PCR sample contained 10 µL OneTaq® Quick-Load® 2X Master Mix (M0486L, New England Biolabs), 1 µL 10 µM forward primer ...
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bioRxiv - Developmental Biology 2024Quote: ... and both reverse transcription and PCR were performed using the Luna One-Step Universal RT-qPCR kit (NEB, E3005S) and carried out on the Mic qPCR Machine (BioMolecular Systems ...
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bioRxiv - Immunology 2022Quote: ... quantitative reverse transcription PCR (qRT-PCR) was conducted using Luna universal probe one-step RT-PCR kit (NEB, #E3006) on CFX96 Touch Real-Time Detection System on 96-well plates (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... P13-P19) (73) were carried out using Luna Universal One-Step RT-qPCR Kit (New England Biolabs; Frankfurt (Main), Germany ...
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bioRxiv - Immunology 2023Quote: ... and the RT-qPCR master mix prepared using a Luna Universal One-Step RT-qPCR Kit (New England Biolabs), as per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by the addition of one µl of Luna Cell Ready Stop Solution 10X (New England Biolabs, Ipswich, MA). We extracted DNA (111 ...
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bioRxiv - Pathology 2023Quote: ... Each 10 µL reaction contained Luna Probe One-Step RT-qPCR 4X Mix (New England Biolabs, Ipswich, MA, USA), primers (10 µM) ...
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bioRxiv - Synthetic Biology 2023Quote: ... and terminator) was assembled in one-pot Type IIS DNA assembly reaction using BsaI-HFv2 (New England Biolabs, NEB). Transcription units to assay the sensor output contained the PQS promoter expressing the gfp gene ...
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bioRxiv - Synthetic Biology 2023Quote: ... and terminator) was assembled in one-pot Type IIS DNA assembly reaction using BsaI-HFv2 (New England Biolabs, NEB). Transcription units to assay the sensor output contained the PQS promoter expressing the gfp gene ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA expression was determined using SYBR green Luna Universal One-step RT-PCR kit (New England Biolabs, Cat #E3005L) with a BioRad CFX96 thermocycler ...
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bioRxiv - Biochemistry 2023Quote: ... In order to remove the EndoHf from the HPAMPD the supernatant was brought to 100 mM Tris -HCl pH 7.5 and 100 mM NaCl and applied to a one ml amylose resin (NEB) column at a rate of about 1 drop per second at 25° C ...
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bioRxiv - Microbiology 2023Quote: ... This was followed by PCR using One Taq Quick-Load 2x Master Mix with Standard Buffer from BioLabs (UK).
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription of RNA and quantitative PCR was performed using the Luna Universal One-step RT-qPCR Kit (NEB) using 1 µg of RNA per reaction ...
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bioRxiv - Microbiology 2024Quote: ... RIG-I and ISG15 were determined using Luna Universal One-Step RT-qPCR Kit according to the manufacturer’s instructions (New England Biolabs). The primer sequences are available upon request ...
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bioRxiv - Molecular Biology 2024Quote: ... All qPCRs were performed using the Luna® Universal One-Step RT-qPCR Kit (New England Biolabs, Ipswich, MA) and a Step One Plus qPCR machine (Life Technologies ...
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bioRxiv - Microbiology 2024Quote: ... treated or not for one hour at 56°C with 0.4 mg/mL proteinase K (P8107S, New England Biolabs) and then heated or not for one hour at 85°C.
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bioRxiv - Genomics 2020Quote: ... 6 μL of DNA was subject to restriction digestion with McrBC (New England Biolabs, US) at 37°C for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... depleted with the NEBNext rRNA Depletion Kit (Human/Mouse/Rat, NEB, Figures 6 and S6), or not depleted (Figures 2B ...
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bioRxiv - Biochemistry 2019Quote: ... The cDNA was amplified for 6-16 cycles (primers below) with Phusion Polymerase (NEB, #E0553L) and gel-purified with ZymoClean Gel DNA Recovery Kit (Genesee Scientific ...
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bioRxiv - Biophysics 2020Quote: ... Digestion mix (84 μl water, 10 μl 10x NEB Buffer 3.1, 6 μl Nt.BspQ1 (NEB)) was prepared and heated to 50 oC for 5 minutes ...
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bioRxiv - Genomics 2019Quote: ... followed by the addition of 0.5 µl of 250 mM Random Primer 6 (S1230S, NEB) and 1 µl Deoxynucleotide Solution Mix (N0447 ...
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bioRxiv - Synthetic Biology 2021Quote: ... 6 were generated by PCR amplification (Phusion High-Fidelity PCR Kit, New England Biolabs #E0553) of an oligo that includes a T7 promoter ...
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bioRxiv - Microbiology 2021Quote: ... and 6 µg was digested with 2U/µg DNA of the restriction endonuclease Sau3AI (NEB). Ligation with 400 U of T4 DNA ligase (NEB ...
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bioRxiv - Genomics 2019Quote: ... The samples were further digested for 6 hr by 25 U of I-SceI (NEB). I-SceI was also heat inactivated ...
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bioRxiv - Biochemistry 2021Quote: C-terminally (His)6-tagged LukE was expressed in BL21 (DE3) Escherichia coli cells (NEB). Transformed cells were grown at 37 °C in Terrific broth supplemented with 100 μg/mL ampicillin to a density of OD600 = 0.6 ...
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bioRxiv - Biophysics 2020Quote: ... Digestion mix (84 μl water, 10 μl 10x NEB Buffer 3.1, 6 μl Nt.BspQ1 (NEB)) was prepared and heated to 50 oC for 5 minutes ...
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bioRxiv - Immunology 2022Quote: ... a 6-Helix construct was expressed recombinantly in BL21 (DE3) Escherichia coli (New England Biolabs). This construct was composed of three NHR and three CHR peptides with intervening glycine/serine linkers and a C-terminal hexahistidine purification tag ...
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bioRxiv - Molecular Biology 2023Quote: ... 6 μL 20mg/ml BSA and 5 μL 400U/μl T4 DNA Ligase (NEB, M0202) overnight at 16°C (tumbling ...
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bioRxiv - Biophysics 2024Quote: ... 55 The samples were mixed with 6 × purple loading dye without sodium dodecyl sulfate (NEB) and with 10 × TBE to make a 1 × solution ...
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bioRxiv - Plant Biology 2024Quote: ... the cDNA was pre-amplified with 6 cycles using Phusion HF Mastermix (New England Biolabs) and size-selected using the ProNex Size-Selective Purification System (Promega) ...
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bioRxiv - Plant Biology 2021Quote: ... The pENTR/D construct was then digested with the MluI restriction enzyme (NEB, Cat# R0198S) and recombined into a modified gateway destination vector harboring Hygromycin drug resistance as described in Law et al ...
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bioRxiv - Cell Biology 2020Quote: ... Full-length 265 nt 5’ UTR of SARS-CoV-2 was subcloned to replace the 5’ UTR of human CMV in the pLV-mScarlet vector using a Hifi one-step kit (Gibson Assembly, NEB). Full-length ...
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bioRxiv - Cell Biology 2019Quote: ... One μg of purified RNA was reverse transcribed into cDNA using the Protoscript II Reverse Transcriptase kit (New England Biolabs). Quantitative real time polymerase chain reactions (qRT-PCR ...
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bioRxiv - Molecular Biology 2021Quote: ... Full-length 265 nt 5′ UTR of SARS-CoV-2 was subcloned to replace the 5’ UTR of human CMV in the pLV-mScarlet vector using a Hifi one-step kit (Gibson Assembly, NEB). Full-length ...
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bioRxiv - Microbiology 2021Quote: ... Official CDC SARS-CoV-2 N1 gene primers and TaqMan probe set were used [58] with the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs):
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bioRxiv - Molecular Biology 2021Quote: ... The mRNA abundance levels were determined using 200 ng total RNA for each sample in technical replicates using Luna Universal One-Step RT-qPCR kit (New England BioLabs) and SYBR Green as detection agent and gene-specific primers (Table S2) ...
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bioRxiv - Cell Biology 2022Quote: ... and were assembled into pHaloTag-C1 to produce the pHalo-Baf in one-step using the Gibson Assembly Master Mix (New England Biolabs). The cGAS cDNA was amplified by the KOD One from pLPC-cGAS-Flag (Dou et al. ...
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bioRxiv - Genomics 2020Quote: ... One microgram of DNase-treated total RNA was reverse transcribed using AMV reverse transcriptase (New England Biolabs, Ipswich, MA, USA) and a gene-specific primer for either the germline-limited gene or actin II control ...
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bioRxiv - Immunology 2021Quote: ... one as Cytosolic Extraction Buffer (CEB) (HEPES 10 mM; KCl 60 mM; EDTA 1 mM; NP40 1%) and another one as Nuclear Extraction Buffer (NEB) (HEPES 20 mM ...
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bioRxiv - Microbiology 2020Quote: Reverse transcription and amplification for figure 2 was performed using OneTaq One-Step RT-PCR Kit from NEB (cat. # E5315S). Both the OneTaq One-Step RT-PCR Kit and Luna Universal One-step RT-qPCR Kit (cat ...
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bioRxiv - Microbiology 2019Quote: ... samples were diluted to 5ng/uL and used for qRT-PCR with Luna One-Step Universal qPCR kit (NEB E3005). Gene specific primers for target transcripts were used at a final concentration of 400uM with 15ng of RNA ...
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bioRxiv - Systems Biology 2020Quote: ... One microgram of purified RNA was fragmented at 95 °C for 7 min in 1X T4 RNA Ligase buffer (NEB) with an equal volume of 2X alkaline fragmentation buffer (0.6 volumes of 100 mM Na2CO3 plus 4.4 volumes of 100 mM NaHCO3) ...