Labshake search
Citations for New England Biolabs :
2051 - 2100 of 3130 citations for Locostatin CAS 90719 30 5 100% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2023Quote: ... (1.33x Gibson-Assembly Master-Mix [for 25 reactions]: 100 μL 5x Isothermal Reaction-Mix, 215.32 μL H2O, 0.2 μL T5 Exonuclease [10 U/μL NEB #M0363] ...
-
Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... resuspended purified nuclei were incubated with 1 mM CaCl2 and 100 ku/ml micrococcal nuclease (New England Biolabs) in HB for 37°C for 15 min ...
-
bioRxiv - Cell Biology 2023Quote: ... beads were equilibrated in 1x PMP buffer (50 mM HEPES, 100 mM NaCl, 2 mM DTT, 0.01% Brij 35, pH 7.5; New England Biolabs). Kinase reactions were performed with 1 μL commercial CK1δ (New England Biolabs ...
-
bioRxiv - Microbiology 2023Quote: ... 50 µg of lysostaphin and 100 µl of the tissue lysis buffer (Monarch Genomic DNA purification kit, NEB). Cells were lysed at 37°C for 30 min and then processed following the manufactures instructions.
-
bioRxiv - Neuroscience 2023Quote: ... Amplicons were then quantified on ethidium bromide stained agarose gels against a DNA marker (100 bp ladder, NEB), pooled and then Illumina sequenced (paired end ...
-
bioRxiv - Cancer Biology 2024Quote: ... Nuclei were treated with 0.5% SDS followed by 1.25% Triton X-100 before digesting with 400 U MboI (NEB) for 4 hr ...
-
bioRxiv - Microbiology 2024Quote: ... resuspended in 100 µL of supernatant and labeled by incubation with SNAP-cell TMR-Star (New England Biolabs) at a final concentration of 250 nM for 30 min at 37 °C in the dark ...
-
bioRxiv - Genomics 2024Quote: ... 1 μl of 100 pmol dCTP and 0.5 μl of terminal transferase (20 units/μl) (New England Biolabs), and the mixture was incubated for 1 hour at 37°C followed by heating for 10 min at 70°C ...
-
bioRxiv - Genomics 2024Quote: ... Nuclei were isolated from 1×106 unfixed cells using PBS 0.1% NP-40 and resuspended in 75 μl of Nuclear Isolation buffer (10mM Tris HCl, 0.1% NaDeOx, 0.1% Triton-100) and 75 μl of 2X MNase digestion Buffer (NEB). Chromatin was digested using MNase (NEB ...
-
bioRxiv - Cancer Biology 2024Quote: 100 ng of extracted and digested genomic DNA was mixed with 7.5 U phi29 DNA polymerase (NEB M0269S), and 1 mM each of dATP ...
-
bioRxiv - Biochemistry 2024Quote: ... A 100 µl master mix was prepared that consisted of the 1X DNA Adenylation Buffer (New England Biolabs), 100 µM ATP ...
-
bioRxiv - Bioengineering 2024Quote: A reaction solution of 100 µL containing 0.32 units/µL Bst DNA polymerase large fragment (New England Biolabs), 0.5 µM forward primer ...
-
bioRxiv - Cell Biology 2024Quote: ... Proximity ligation in the sample was performed using 100 U of added T4 DNA ligase (New England Biolabs) at 16°C for 16 hr ...
-
bioRxiv - Microbiology 2020Quote: ... Following KLD (kinase, ligase, and DpnI) treatment and transformation into NEB 5-alpha F’Iq cells (New England Biolabs), cells were grown as near-lawns on LB-chloramphenicol plates and a miniprep was performed from a resuspension of ~5000 colonies for each library.
-
bioRxiv - Genomics 2021Quote: ... the purified products are treated with Klenow fragment (3’ → 5’ exo-) (Cat. No. M0212L; NEB; use 1 uL) and Taq DNA polymerase (Cat ...
-
bioRxiv - Biochemistry 2020Quote: ... the top strand (MB733) was 5′ labelled with ϒ[32P]- ATP and T4 polynucleotide kinase (T4 PNK; NEB) for 1 h at 37°C and cleaned up with a G-50 micro column as above ...
-
bioRxiv - Developmental Biology 2021Quote: ... the synthesized sgB was injected at 300ng/µL after 5-minute incubation at RT with Cas9 protein (NEB) at 2µM final in 20mM Hepes-NaOH pH 7.5 ...
-
bioRxiv - Developmental Biology 2021Quote: ... 5 μL (approximately 250 ng) of the extracted DNA was first digested using the restriction enzyme DpnII (NEB), diluted to 100 μL and ligated with T4 ligase (NEB) ...
-
bioRxiv - Developmental Biology 2020Quote: ... 37.5 μl of 0.4 mM biotin-14-dATP and 10 μl of 5 U/ μl Klenow (NEB, M0210L) were added and mixed by pipetting ...
-
bioRxiv - Molecular Biology 2020Quote: ... The RNA was then 5’ end labeled in 24μL PNK wash buffer with 16μL of 1x PNK buffer (NEB) containing 150μCi of gamma P32-ATP ...
-
bioRxiv - Neuroscience 2021Quote: NFIB 3’ UTR and 5’ UTR HP forming regions were in vitro transcribed using T7 transcriptase (NEB, E2040) and purified with Trizol extraction (described in RT-qPCR paragraph) ...
-
bioRxiv - Microbiology 2020Quote: ... RNA was denatured again and pyrophosphates were removed from the 5’-end by 10 U RppH (M0356S, NEB) in 1× NEB buffer 2 (10 mM Tris-HCl pH 7.9 ...
-
bioRxiv - Synthetic Biology 2020Quote: ... was achieved by PCR of the relevant design using primers F_RiboJ_Rem and R_RiboJ_Rem (Supplementary Table 5) and subsequent circularization by standard Golden Gate assembly (New England Biolabs, E1601S) to create the plasmids pVB003 and pVB004 ...
-
bioRxiv - Biophysics 2020Quote: ... Fluorescence labelling was done by incubating with 5 nmol SNAP-Surface Alexa Fluor 488 (#S9129S, New England Biolabs) for 1 hour at 4 °C ...
-
bioRxiv - Genomics 2020Quote: ... RNA was resuspended in 5 μl of water and the 3′ ends dephosphorylated with PNK (New England BioLabs) in MES buffer (100 mM MES-NaOH ...
-
bioRxiv - Plant Biology 2020Quote: ... were amplified from genomic DNA using primers in Supplemental Table 5 and Taq Phusion polymerase (New England BioLabs), cloned in pGEM-t Easy (Promega) ...
-
bioRxiv - Microbiology 2020Quote: ... 15 μg of DNA-depleted RNA was incubated with 12.5 units of RNA 5’ pyrophosphohydrolase (RppH) (#M0356, NEB) in a 50 μl reaction for 1 h at 37°C ...
-
bioRxiv - Microbiology 2021Quote: ... the cap-1 structure was added to the 5’ end using the Vaccinia capping enzyme (New England Biolabs) and Vaccinia 2’-O-methyltransferase (New England Biolabs) ...
-
bioRxiv - Genomics 2020Quote: ... Chromatin within intact nuclei was then digested for 5 minutes with micrococcal nuclease (NEB #M0247, 500U/1M cells) prior to nuclei lysis by Covaris sonication ...
-
bioRxiv - Biochemistry 2021Quote: ... gDNA was quantified by Nanodrop and a fraction of DNA was treated with 5 U RNase H (NEB) for 1h at 37 °C ...
-
bioRxiv - Immunology 2020Quote: ... Residual template switch adaptor was removed by incubation with 5 U of uracil DNA glycosylase (New England BioLabs) for 40 min at 37°C ...
-
bioRxiv - Microbiology 2021Quote: ... and was then installed with 5’cap (Vaccinia Capping System, NEB, USA; Cap 2’-O-methyltransferase, NEB, USA) and 3’ Poly(A ...
-
bioRxiv - Biochemistry 2022Quote: 3’-PUA-DNA was generated by incubation of 1 μM 5’-FAM-labeled AP-DNA (FAM_U_35) with 20 U EndoIII/Nth (NEB) in Buffer B pH 7.0 at 37 °C for 60 m ...
-
bioRxiv - Microbiology 2022Quote: ... 2.5µL of 10µM MBTUni-12 primer + 2.5µL of 10µM MBTUni-13 primer (5’-ACGCGTGATCAGTAGAAACAAGG-3’) + 10µL 5x HF Phusion Buffer + 1µL 10mM dNTPs mix (NEB #N0447S) + 0.5µL Phusion Polymerase + 28.5µL of Nuclease-free water (Ambion ...
-
bioRxiv - Biochemistry 2021Quote: ... 5 µL of PCR product from the inner primer amplification was cleaned used 0.5 µL Exonuclease I (NEB) and 1 µL rSAP (NEB) ...
-
bioRxiv - Neuroscience 2020Quote: ... Samples were incubated on ice for 5 minutes then immediately treated with appropriate concentration of RNAse If(NEB) for 3 mins at 37C at 1200RPM ...
-
bioRxiv - Cell Biology 2021Quote: ... 5 µg antibody (Table. S3) and 50 µl protein A magnetic beads (New England Biolabs, Cat. No. S1425S) were added to the supernatant and incubate overnight at 4 degree Celsius ...
-
bioRxiv - Molecular Biology 2020Quote: ... Sample was pelleted at 2500xg for 5 minutes and the pellet was washed with 1x Cutsmart buffer (NEB) and pelleted again ...
-
The absence of C-5 DNA methylation in Leishmania donovani allows DNA enrichment from complex samplesbioRxiv - Molecular Biology 2020Quote: ... and cloned between 300 bp of PCR amplified DNA fragments of the LdBPK_250018100.1 5’ and 3’ UTR using NEBuilder (NEB) inside pUC19 for construct amplification in E ...
-
bioRxiv - Immunology 2020Quote: ... and the assembled plasmid pCO1 was transformed into NEB® 5-alpha competent Escherichia coli (High Efficiency) (NEB) according to manufacturer’s instructions (see Supplementary Figure 1 for plasmid maps) ...
-
bioRxiv - Molecular Biology 2020Quote: ... The cDNA reaction mix was therefore compensated by adding 0.5 μl 5’Ligation Reaction Buffer 10x (NEB-kit) and 1.75 μl nuclease-free H2O.
-
Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... Pre-adenylated DNA linker(DNA oligo purchase from IDT was adenylated using 5′ DNA Adenylation Kit (NEB,E2610S)) was ligated to the 3’-end of RNA in a 20 µl reaction (25% PEG8000 ...
-
bioRxiv - Molecular Biology 2021Quote: ... by AgeI and EcoRI digestion and ligation of 5’-phosphorylated DNA duplexes using T4 DNA ligase (NEB, #M0202). Target plasmids expressing 0 and 480 CTG repeats in the context of DMPK exons 11-15 (DMPKS and DT480 ...
-
bioRxiv - Molecular Biology 2021Quote: ... R: 5’-ccgggaaaaactgaaaaaccattggcacgacaggtttcccgac-3’ from the pKAM555 vector) was inserted at the ClaI site using HiFi assembly (NEB). For the intTEL0 strain creation the pUC19LG plasmid (lacking the PstI(blunted)-BclI fragment of the HARS36 sequence ...
-
bioRxiv - Biophysics 2021Quote: ... The phosphorylated 5’-ends were subjected to RNA ligation by 20 units of T4 RNA ligase 1 (NEB) in the presence of 10 mM DNA/RNA rP5_RND oligo (Supplemental Table 3 ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1.5 μl 10 mM dNTPs, 0.5 μl 10 μM NEB Universal PCR primer, 0.5 μl 10 μM NEB index primer ...
-
bioRxiv - Molecular Biology 2021Quote: Total RNA (5 μg) was ligated to the RNA 3’ adaptor using T4 RNA Ligase 2 - truncated (NEB), in the presence of RNase Inhibitor (NEB) ...
-
bioRxiv - Immunology 2020Quote: ... TAP reaction was performed in a total volume of 25 μL using 5 μL of Q5 polymerase (NEB), 5 μL of GC Enhancer (NEB) ...
-
bioRxiv - Molecular Biology 2022Quote: ... 1.5 μl 10 mM dNTPs, 0.5 μl 10 μM NEB Universal PCR primer, 0.5 μl 10 μM NEB index primer ...
-
bioRxiv - Molecular Biology 2022Quote: ... 1.5 μl 10 mM dNTPs, 0.5 μl 10 μM NEB Universal PCR primer, 0.5 μl 10 μM NEB index primer ...