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Citations for New England Biolabs :
2001 - 2050 of 3130 citations for Locostatin CAS 90719 30 5 100% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... and amplified for 5 cycles using NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs; M0541L). After evaluation by real-time PCR ...
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bioRxiv - Microbiology 2023Quote: ... CVO689-CVO586 (integrant 5’ end) and CVO321-CVO183 (integrant 3’ end) using Q5 Polymerase (New England Biolabs). PCRs were set up according to manufacturers’ instructions ...
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bioRxiv - Physiology 2024Quote: ... Glycan digestion was achieved with the following enzyme concentrations: 5 μL of α2-3,6,8,9 neuraminidase A (20 U/μL, New England Biolab, NEB) with 1x GlycoBuffer 1 (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... then combined with 5 μM dNTPs and 2 μL of 10× rCutSmart buffer (NEB, Ipswich, MA, USA) in a total reaction volume of 17 μL ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Nuclei were pelleted at 500 ξ g at 4°C for 5 minutes and resuspended in 0.5 mL of 1.2x NEBuffer r2.1 (New England Biolabs) containing 3% sodium dodecyl sulfate (SDS ...
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bioRxiv - Plant Biology 2024Quote: ... as described in the NEB Golden Gate Assembly protocol (5-10 inserts protocol) (NEB, MA, United States). E ...
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bioRxiv - Molecular Biology 2024Quote: ... the gBlock corresponding to the HIV-1C 5’ LTR was digested with EcoRⅠand BssHⅡ (New England Biolabs), which were also used to digest the pEV731 vector ...
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bioRxiv - Neuroscience 2024Quote: ... 50 µg of protein lysate was treated with 5 µL NeuA (a2-3,6,8,9 Neuraminidase A, P0722L, NEB) or 5 µL of O-Glycosidase (P0733L ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The assembled plasmid was transformed into NEB 5-alpha (high efficiency) competent cells (New England Biolabs; C2987). After verifying the sequence ...
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bioRxiv - Bioengineering 2024Quote: ... Initial studies were done in an alternate NEB® 5-alpha strain (New England Biolabs, Catalog # C2987H) where trpA and trpB were replaced with a chloramphenicol resistance cassette ...
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bioRxiv - Molecular Biology 2024Quote: ... fragments were dephosphorylated by adding 5 μL antarctic phosphatase buffer and 2 μL enzyme (NEB cat#M0289S) and incubating for 1 h at 37°C ...
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bioRxiv - Genetics 2024Quote: 48 h post-electroporation cells were spun down (300G 5’) and resuspended in DNase I (NEB M0303L) at 37C for 15’ using 10 U of enzyme per ug of plasmid transfected in a final volume of 2 ml of DPBS to eliminate possible carryover plasmid in the exterior of the cells ...
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bioRxiv - Molecular Biology 2024Quote: ... U2OS cells expressing SNAP-H3.1 or SNAP-H3.3 were incubated with 5 µM SNAP-Cell Block (NEB) for 30 min at 37°C for the quench step ...
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bioRxiv - Biochemistry 2024Quote: ... 5 µl of each sample was mixed with 1 µl of 6X Purple Gel Loading Dye (NEB) and electrophoresed at 110 V for 30 minutes ...
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bioRxiv - Synthetic Biology 2024Quote: ... UK - protocol version ACDE_9163_v114_revO_29Jun2022) and 5 µL of Quick-NEBNext Quick Ligation Module Quick Ligase (NEB, UK) incubated for 10 minutes at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... with RBNS RT primer (Supp. Table 5) and PCR amplified via Phusion DNA polymerase (New England Biolabs) with RBNS index primers and RBNS reverse primer (Supp ...
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bioRxiv - Cell Biology 2024Quote: ... Embryos were injected with 1 mg of gRNA and 5 μ M of Cas9 protein (NEB, 10128356) at 1-cell stage ...
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bioRxiv - Molecular Biology 2021Quote: ... and KpnI) into pSB/IR-CA-HybIntr-SV40intr-BleoR-T2A-mCherry plasmid restricted with restriction endonucleases BsaI (NEB, USA) and AsiG.
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bioRxiv - Cancer Biology 2021Quote: ... Whole-genome sequencing and library preparations for tumors and matched germline controls were performed according to the manufacturer’s instructions (Illumina, San Diego, CA, USA or NEBNext, NEB). The quality of the libraries was assessed using a Bioanalyzer (Agilent ...
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bioRxiv - Synthetic Biology 2022Quote: ... sgRNA expression plasmids were constructed via digesting the sgRNA backbone plasmids that contained a human U6 promoter and a Cas-nuclease’s corresponding sgRNA scaffold sequences using BsmB I or Bbs I endonuclease (NEB) and then ligated the oligonucleotide duplexes into this cut backbone ...
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bioRxiv - Cancer Biology 2023Quote: Lentiviral vectors for inducible expression of CA-MNK2 (T379D) and SLUG were generated by Gibson assembly (New England Biolabs) or standard cloning in the pTRIPZ plasmid from pTK-Slug (a gift from Robert Weinberg ...
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bioRxiv - Genomics 2022Quote: ... Library preparation was performed using NEB Next® Ultra™ RNA Library Prep Kit (NEB, San Diego, CA, USA), employing AMPure XP Beads to purify the products of the reactions during the library prep ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were purified with DNA Spin and Concentrate kit (Zymo Research, Irvine, CA, D4013 or NEB Monarch, T1030) following manufacturer instructions or gel-purified from kit (Zymo Research) ...
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bioRxiv - Neuroscience 2024Quote: ... Supernatants were discarded and nuclei pellets were washed once with 4 mL Nuclei EZ Lysis Buffer and once with 4 mL of Nuclei Suspension Buffer (NSB) (PBS w/o Mg and Ca; 0.01% BSA; Biolabs ref B9000S ...
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bioRxiv - Molecular Biology 2024Quote: The C-type CA sequence was cloned into pCMVHTLV_delta env (kind gift from Dimitry Mazurov) by Gibson Assembly (NEB) and all subsequent point mutations were introduced by standard overlapping PCR ...
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bioRxiv - Molecular Biology 2020Quote: ... 100 ng of unlabeled crRNA was mixed with equal volume of 2 × RNA loading dye (New England Biolabs) and fractionated in the 12% denaturing polyacrylamide gel ...
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bioRxiv - Cell Biology 2020Quote: ... Proximity ligation in the sample was performed using 100 U of added T4 DNA ligase (New England Biolabs) at 4°C for 16h ...
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bioRxiv - Developmental Biology 2020Quote: 5-7 μg of HiC library in a total volume of 100 μl (1x NEB buffer 2.1, 0.025 mM dNTPs, 0.12 U/ μl T4 DNA polymerase (NEB, M0203) was incubated at 20°C for 4 h to remove biotin from unligated ends ...
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bioRxiv - Molecular Biology 2020Quote: ... the cells were permeabilized in 0.5% Triton X-100 (Acros) in 1xPBS with 2mM Vanadyl Ribonucleoside Complex (NEB) for 10-20 minutes on ice ...
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bioRxiv - Genomics 2020Quote: ... Then the beads were equilibrated once in 100 uL 1x NEB T4 DNA ligase buffer (New England Biolabs) followed by removal of the supernatant ...
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bioRxiv - Physiology 2020Quote: Input RNA (50-100 ng/µL) was reverse-transcribed using LunaScript® RT SuperMix Kit (New England BioLabs) or TaqMan MicroRNA Reverse Transcription Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Two 100 μl aliquots were incubated overnight at 37°C with and without Endo H (3000 U, NEB). Then the samples were heated with 100 μl SDS sample buffer for 5 min at 95°C.
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bioRxiv - Genomics 2021Quote: ... phage particles were lysed at 56 °C for 2 h in 550 μL of lysis buffer (100 mM Tris-HCl at pH 8.0, 27.3 mM EDTA, 2% SDS, ~1.6 U Proteinase K [NEB #P8107]). After lysis ...
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bioRxiv - Systems Biology 2020Quote: Single hematopoietic stem and progenitor cells were index-sorted into 384 well plates containing 0.5 µl of nuclease free water with 0.2% Triton-X 100 and 4 U murine RNase Inhibitor (NEB), spun down and frozen at −80°C ...
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bioRxiv - Biochemistry 2022Quote: ... The mixture was then added to 100 µL of NEBNext® Sample Purification Magnetic Beads (New England Biolabs) and incubated at room temperature for 5 min ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR was performed using 100 ng of genomic DNA and Q5 High-Fidelity Taq Polymerase (New England Biolabs) was used according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... fixed cells were incubated in DNAse I buffer (New Englab BioLabs, M0303S, 1:100 dilution in PBS buffer) for 30 min at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... Oligonucleotide pairs were resuspended to 100 μM and annealed in a polynucleotide kinase (PNK) mix (New England BioLabs) consisting of 1.5 μL of each oligonucleotide ...
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bioRxiv - Cancer Biology 2020Quote: ... 2.5μL of 20% Triton X-100, 1μL BSA (100x, 10mg/mL) and 10μL HaeIII (10U/μL, NEB, R0108S) with shaking at 37°C overnight ...
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bioRxiv - Immunology 2021Quote: ... into 384 well plates containing 0.5 µl of nuclease free water with 0.2% Triton-X 100 and 4 U murine RNase Inhibitor (NEB), spun down and frozen at −80°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 μl of 100 μM SARS_139 and SARS_140 primers were mixed with 1 μl T4 Polynucleotide kinase (PNK; NEB) in 1x PNK reaction buffer (NEB) ...
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bioRxiv - Cell Biology 2023Quote: ... 100 nM okadaic acid (Enzo LifeSciences, ALX-350-011-M001) 50 U micrococcal nuclease (New England Biolabs, M0247S)) ...
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bioRxiv - Molecular Biology 2022Quote: ... The reverse transcriptase reaction was performed using between 100 ng and 1 μg RNA per sample (NEB, E3010). Quantitative PCR (qPCR ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 ng of DNA template in 25 µl of 1x One Taq Standard Buffer (Biolabs, Ipswich, MA, USA), 0.2 mM dNTPs ...
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bioRxiv - Biochemistry 2024Quote: ... cells were harvested 3 days after electroporation and genomic DNA was extracted with 100 μL lysis buffer (10 mM Tris-HCl, pH 7.5, 0.05% SDS, 25 μg/mL proteinase K (NEB)) at 37 °C with incubation for 1 hour ...
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bioRxiv - Genomics 2024Quote: ... RNA (100 ng) was used for cDNA synthesis using the ProtoScript II First Strand cDNA Synthesis Kit (NEB) with random hexamers ...
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bioRxiv - Neuroscience 2023Quote: ... Amplicons were then quantified on ethidium bromide stained agarose gels against a DNA marker (100 bp ladder, NEB), pooled and then Illumina sequenced (paired end ...
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bioRxiv - Molecular Biology 2022Quote: ... For size reference 1 kb and 100 bp DNA ladders (New England Biolabs, catalog no. N3232 and N3231) were combined and loaded onto the gel ...
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bioRxiv - Biophysics 2022Quote: ... 1 μL of 100 mM 18O4-γ-ATP followed by addition of 20 U of T4-PNK (NEB) and incubation for 1 h at 37 °C ...
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bioRxiv - Biophysics 2022Quote: ... 40 μL of pooled oligonucleotides (100 μM) were mixed with 12 μL ddUTPs (1 mM, New England Biolabs), 2.4 μL TdT (20000 U/mL ...