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Citations for New England Biolabs :
2301 - 2350 of 3130 citations for Locostatin CAS 90719 30 5 100% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... After centrifugation the EtOH-precipitated fragments were resuspended in water and ligated to 0.25 µM randomized 5’ RNA adapter using T4 RNA ligase 1 (NEB) in the same buffer conditions as described above ...
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bioRxiv - Systems Biology 2023Quote: ... with 10 µL RT mix (5 µL 5x Maxima RT Buffer, 1.25 µL 10 mM/each dNTP [New England Biolabs #N0447S] ...
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bioRxiv - Neuroscience 2023Quote: ... Ligation of 5’ adaptor to already 3’ ligated RNA product was performed with T4 RNA ligase 1 (NEB, #EL0021) at 37°C for 1 h ...
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bioRxiv - Biophysics 2023Quote: ... Reaction was terminated with 3 μL of stop buffer (5% sarkosyl, 0.025% bromophenol blue, 50% glycerol) and incubated with 1.6 units of Proteinase K (NEB, P8107S) for 15 minutes at 37 degrees before resolving on 1% agarose TAE gel stained with SybrSafe (Invitrogen ...
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Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)bioRxiv - Cell Biology 2023Quote: ... The quantitative PCR reaction mix contained 5 µl of the Luna Universal qPCR Master Mix (2X, New England BioLabs), 1 µl of the forward primer (10 µM) ...
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bioRxiv - Molecular Biology 2023Quote: ... A 5′ adapter (rArCrArCrUrCrUrUrUrCrCrCrUrArCrArCrGrArCrGrCrUrCrUrUrCrCrGrArUrCrU, IDT) was then ligated to RNAs to the product using T4 RNA ligase 1 (NEB) for 4 hours at 15°C ...
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bioRxiv - Molecular Biology 2023Quote: ... DNase-treated RNA (10 µg) was fragmented by using 5 units of ShortCut RNase III (New England Biolabs, M0245) for 5 min at 37°C and the reaction was stopped by adding 10 µL nuclease free water ...
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bioRxiv - Bioengineering 2022Quote: ... the 5S and Saba PCR products were purified using Monarch® PCR & DNA Cleanup Kit (5 μg) (NEB, # T1030). The purified DNA sequence was then analyzed through Sanger sequencing (GENEWIZ from Azenta ...
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bioRxiv - Genetics 2022Quote: ... 1 µL of extracted genomic DNAs and 5 µL of 2× Hot Start High Fidelity Q5 Master Mix (NEB). PCR reactions were carried out under the following condition ...
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bioRxiv - Biochemistry 2023Quote: ... A linker RNA oligonucleotides purchased from IDT was preadenylated at 5’ -end by T4 RNA ligase I (NEB 0437) using a method described previously (30) ...
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bioRxiv - Developmental Biology 2023Quote: ... tissues were then washed twice in DNAse I buffer (66mM Tris pH 7.5 and 5mM MgCl2) for 5 minutes each and then incubated with 10U DNAse (M0303L; New England Biolabs) in 100ul of DNAse buffer at 37 °C for 1hrs ...
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bioRxiv - Genomics 2023Quote: ... nuclei were sorted into a tube of a PCR tube strip containing 5 μL 1X CutSmart buffer (NEB, B7204S) per well ...
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bioRxiv - Genetics 2023Quote: ... and F2 crispants were fin clipped at 5 weeks and genotyped by a T7EI assay (M0302, New England Biolabs). We extracted DNA using the Quick-DNA Microprep Kit (D3021 ...
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bioRxiv - Developmental Biology 2023Quote: ... along with 2.5 ng/μl pCFJ90(Pmyo-2::mCherry) and 77.5 ng/μl of 2-Log DNA ladder (New England BioLabs), to generate two independent strains expressing Ex[Pcol-10::mCherry::his-58::SL2::drl-1 cDNA] (DLS515 ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 pmol of TP_MAAAPQKCAAA* mRNA (see “Toeprinting assays” section) and components of the PURExpress ΔRibosomes Kit (New England Biolabs). The reaction was incubated at 37ºC for 20 min and then diluted in 50 mM Hepes KOH pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... then ligated to a custom 5′ adapter (10 μM oligocap) by T4 RNA Ligase I (M0437, New England Biolabs) with 1 μl RNaseOUT for 16 hours at 16°C ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was isolated and regions flanking Exon 5 were PCR-amplified using Q5 High-Fidelity DNA Polymerase (NEB) (oligonucleotide primers F ...
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bioRxiv - Cancer Biology 2023Quote: ... were then denatured at 95°C for 5 min and cooled at room temperature to form heteroduplexes using NEBuffer2.1 1x (NEB). The T7 endonuclease I (NEB ...
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bioRxiv - Microbiology 2023Quote: ... the 5’-PPP RNA in the total RNA was specifically capped with 3’-Desthiobiotin-GTP (New England BioLabs, N0761) by the Vaccinia Capping System (New England BioLabs ...
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bioRxiv - Developmental Biology 2024Quote: ... along with 2.5 ng/μl pCFJ90(Pmyo-2::mCherry) and 77.5 ng/μl of 2-Log DNA ladder (New England BioLabs), to generate the DLS327 and DLS330 strains ...
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bioRxiv - Genomics 2024Quote: ... Two cycles of whole genome amplification were performed using 50 U of Klenow Fragment (3’→5’ exo-) (NEB, M0212M), dNTP solution mix (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... The RT RNA sample (5 μL) was amplified using the LongAmp Taq 2× Master Mix (NEB, Ipswich, MA, USA) with IVT Nanopore T7 Fw and IVT Nanopore T7 Rv primers (Supplementary Table 5) ...
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bioRxiv - Developmental Biology 2024Quote: ... 2020) and 5’Ncol1::mScarlet::3’Ncol119 plasmids using Gibson assembly according to the manufacturer recommendations (NEB, CAT#E2611). Sequences of Gibson primers used were:
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bioRxiv - Immunology 2024Quote: ... Total RNA samples (5-500 ng) were hybridized with NEBNext rRNA Depletion Kit v2 (Cat# E7400; New England Biolabs) to diminish rRNA from the samples ...
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bioRxiv - Molecular Biology 2024Quote: ... A circular form of the 3I_RAN FLEXI synthetic oligonucleotide control was generated by incubating the 5’ phosphorylated-3I_RAN FLEXI oligonucleotide (500 ng) with T4 RNA Ligase I (10 U; New England Biolabs) for 2 h at 25°C and 2 min at 95°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... compatible overhangs for ligation were generated by digestion of PCR products using 5 units each of BsaI (NEB, R3733S) and DpnI (NEB ...
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bioRxiv - Biochemistry 2024Quote: RP library construction in brief: Footprint RNA was dephosphorylated using 5 U of T4 PNK (New England Biolabs, M0201S). Subsequently ...
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bioRxiv - Molecular Biology 2024Quote: ... 1.5 μg each of vector pNeae2.1 and insert DNA were combined with 5 μL of 10x T4 Ligase Buffer (NEB), 5 μL of 10x rCutSmart Buffer ...
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bioRxiv - Microbiology 2024Quote: ... Next, the DNA adenylated oligonucleotide adenylate intermediate was de-adenylated (RNA sample, NEB Buffer 2, and 5’-deadenylase (NEB); incubation at 30 °C for 1 h ...
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Recruitment of the m6A/Am demethylase FTO to target RNAs by the telomeric zinc finger protein ZBTB48bioRxiv - Molecular Biology 2024Quote: ... The immunoprecipitated RNA was 5’-end-labeled with 32P using T4 polynucleotide kinase (New England Biolabs catalogue number M0201L). Protein-RNA complexes were separated using 4–12% BisTris–PAGE and transferred to a nitrocellulose membrane (Protran) ...
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bioRxiv - Biochemistry 2024Quote: ... unlabeled RNAs were dephosphorylated at the 5’-end by treating with calf intestinal alkaline phosphatase (CIP, New England Biolabs) for one hour at 37 °C ...
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Recruitment of the m6A/Am demethylase FTO to target RNAs by the telomeric zinc finger protein ZBTB48bioRxiv - Molecular Biology 2024Quote: ... The immunoprecipitated RNA was 5’-end-labeled with 32P using T4 polynucleotide kinase (New England Biolabs catalogue number M0201L), separated using 4–12% BisTris–PAGE and transferred to a nitrocellulose membrane (Protran) ...
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bioRxiv - Cancer Biology 2024Quote: ... was used for tagmentation and amplified with Nextera Ad1_noMX and Ad2.X primers (Supplementary Table 5) using 2x Phusion high-fidelity PCR mix (NEB). AMPure beads (Beckman coulter ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 rounds of PCR were run using Q5® Hot Start High-Fidelity 2X Master Mix (NEB cat #M0494) following the manufacturer’s instructions with a 63°C annealing temperature and 30s extension ...
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bioRxiv - Immunology 2024Quote: ... 0.5 µl of 2 µM UPA-long and 0.25 µl Phusion Hot Start Flex DNA Polymerase (New England Biolabs) were added to 15.25 µl nuclease-free water ...
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bioRxiv - Biochemistry 2024Quote: The 9N_VRA3 adapter oligonucleotide (0139, Supplementary Table 2) was pre-adenylated with the 5’ DNA Adenylation Kit (New England Biolabs) using the following protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1–5 μg of plasmid was linearized prior to transformation using either ScaI-HF or EcoRI-HF (both NEB), respectively ...
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bioRxiv - Genomics 2024Quote: ... uLibs remained unmodified while mLibs were methylated by mixing 250 ng of DNA with 5 µl 1.6 mM SAM (NEB), 5 µl 10x NEBuffer2 and 1 µl M.SssI (NEB ...
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bioRxiv - Genomics 2024Quote: ... we performed PCR2 reactions (10,000gRNAs per 50ul reaction) using 5 ul of the pooled PCR1 product per reaction with Q5 polymerase (NEB). Each biological sample was amplified with PCR2 primers with unique barcodes (Supplementary Table 1) ...
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bioRxiv - Biochemistry 2024Quote: ... 10 µg of pUC19 was cleaved with EcoRI and labeled at the 5′ ends using T4 polynucleotide kinase (NEB) with [γ-32P]-ATP or at the 3′ ends using Klenow (NEB ...
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bioRxiv - Genetics 2024Quote: ... The PCR fragments were purified using Monarch® PCR & DNA Cleanup Kit (5 μg) from New England Biolabs (NEB) Cat#T1030L ...
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bioRxiv - Systems Biology 2024Quote: ... complete Protease Inhibitor EDTA-Free) with 5 minutes nutation and then resuspended in tagmentation buffer (1x rCutSmart Buffer NEB #B6004S ...
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bioRxiv - Bioengineering 2024Quote: ... Genomic DNA was dephosphorylated before Cas9 cleavage and dA-tailing (5 μg gDNA, 3 μL Quick CIP (NEB, M0525S), 3 μL 10X rCutSmart buffer (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... the free DNA fragments were purified using a Monarch PCR & DNA Cleanup Kit (5 μg) (New England Biolabs, #T1030S) and quantified ...
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bioRxiv - Bioengineering 2021Quote: ... homology arms were amplified from genomic DNA and introduced to the MCS of the linearized SIBR-Cas plasmid using the NEBuilder® HiFi DNA Assembly Master Mix (NEB). The plasmid was linearized using Esp3I (NEB) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... about 1000 bp upstream and downstream of the cas operon was amplified from MGH21 using Q5 DNA polymerase (NEB, Cat# M0492). Overlap extension PCR was used to fuse these two pieces of DNA to generate a ~ 2000 bp fragment which was then ligated to pKOV vector51 using BamHI and NotI sites ...
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bioRxiv - Cancer Biology 2023Quote: DNA from in vitro and in vivo experiments was sent to Novogene (Sacramento, CA) and library preparation was performed using the NEBNext® DNA Library Prep Kit (New England Biolabs) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... For ssg complementation we PCR amplified the ssg coding sequence and 100 bp upstream sequence (including the ssg native promoter) using Q5 DNA polymerase (New England Biolabs). We cloned this 1057 bp product into KpnI-BamHI digested pUC18T-miniTn7-Gent by Gibson assembly using NEBuilder HiFi Assembly master mix (New England Biolabs ...
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bioRxiv - Genetics 2021Quote: ... which was followed by 37°C for 30 min with addition of 50ul 10% Triton-X 100 to quench SDS and 37°C overnight with addition of 40ul AluI (R0137L, NEB) total ...
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bioRxiv - Molecular Biology 2021Quote: ... and washed twice with 1X T4 DNA ligase buffer before resuspending in 100 μl 1X T4 DNA ligase buffer with 1600 Units T4 DNA ligase (NEB). In nucleo ligation was carried out overnight at 16°C without shaking before confirming that high molecular weight products were obtained ...