Labshake search
Citations for New England Biolabs :
2001 - 2050 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... Amino acid substitutions were generated with the Q5 site-directed mutagenesis kit (NEB) with specific primers (Supplemental Table 4) ...
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bioRxiv - Molecular Biology 2020Quote: ... and quantified by qPCR using NEBNext Library Quant kit for Illumina (NEB, E7630). Equal molarity of each library were pooled and subjected to sequencing on an Illumina HiSeq 4000 platform at the McGill University and Génome Québec Innovation Centre to generate 50 bp paired-end reads.
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bioRxiv - Microbiology 2021Quote: ... according to the NEBNext Ultra DNA Library Prep kit instructions (New England Biolabs). Libraries were quantified by Qubit (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... using a NEBNext® Ultra Directional RNA Library Prep Kit (New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 12.5 μl was purified using a silica column-based kit (New England Biolabs, cat#T1030S ...
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bioRxiv - Neuroscience 2020Quote: ... gRNA transcription was performed with HiScribe T7 High Yield RNA Synthesis Kit (NEB) according to the manufacturer’s protocol and gRNAs were purified via Phenol:Chloroform:Isoamyl alcohol (25:24:1 ...
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bioRxiv - Microbiology 2020Quote: ... Products were purified using the Monarch DNA gel purification kit (New England Biolabs), and Sanger sequenced.
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bioRxiv - Genomics 2020Quote: ... Unmethylated pUC19 was extracted using the Monarch Plasmid Miniprep Kit (NEB, Ipswich, MA). 10 μg of pUC19 was CpG methylated in vitro using 40 units of M.SssI (NEB ...
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bioRxiv - Immunology 2021Quote: ... Libraries were constructed using NEBNext Ultra II Directional RNA Library Prep Kit (NEB) according to the manufacturer’s protocol and barcoded using the NEBNext Multiplex (unique dual index ...
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bioRxiv - Bioengineering 2020Quote: ... IVT was performed overnight at 37°C using the HiScribe T7 kit (NEB) following the manufacturer’s instructions (N1-methyl-pseudouridine modified) ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA extraction was performed using the Monarch total RNA Miniprep Kit (NEB, T2010S) following the manufacture recommendations and RNA samples were stored at -80°C until use.
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bioRxiv - Genetics 2022Quote: ... Each of these fragments were extracted using a DNA Gel Extraction Kit (NEB), quantified ...
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bioRxiv - Microbiology 2022Quote: ... The RNA was then extracted using a Monarch total RNA miniprep kit (NEB) as per manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Mutagenesis was performed with a Q5 site directed mutagenesis kit (New England Biolabs) or using a modified QuikChange™ method31.
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bioRxiv - Genomics 2022Quote: ... and TruSeq adapters were ligated using the NEBNext Quick Ligation kit (NEB #E6056). Libraries were amplified by PCR using Kapa HiFi DNA polymerase (10-14 cycles) ...
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bioRxiv - Genetics 2022Quote: ... before library preparation using the NEBNext Ultra II DNA library prep kit (NEB). Samples were indexed for either single or dual-index sequencing ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from cells using Monarch total RNA Miniprep kit (NEB). cDNA synthesis was performed using LunascriptTM RT Supermix Kit (NEB ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we used NEBNExt Ultra II Directional RNA Library Prep Kit for Illumina (NEB). Organ-specific RNA-seq was carried out on a Hiseq4000 and embryonic stage specific RNA-seq was done on a Novaseq6000 SP ...
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bioRxiv - Bioengineering 2022Quote: ... PCR fragments were then purified using a gel extraction kit (New England Biolabs) and subjected to sanger sequencing ...
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bioRxiv - Molecular Biology 2022Quote: ... and the products were gel-purified using the Monarch Gel Purification Kit (NEB) following the manufacturer’s instructions and eluted in 40 μL H2O ...
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bioRxiv - Molecular Biology 2022Quote: ... or HiScribe™ T7 mRNA Kit with CleanCap® Reagent AG (NEB E2080S) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... One microgram of RNA was ribodepleted using NEBNext rRNA Depletion Kit (NEB #E6310). Ribodepleted RNA was fragmented ...
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bioRxiv - Microbiology 2022Quote: ... DNA fragments were then concentrated on columns (Monarch PCR & DNA Cleanup Kit, NEB). The size of the DNA fragments generated (150-400 bp ...
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bioRxiv - Cell Biology 2020Quote: ... Internal deletions of GCP6 were constructed using the Gibson kit (NEB, Ipswich, MA), whereas N-terminal deletions were generated by PCR (using restriction sites BamH1 and Ale1 in the sequences of the vector or the GCP6 DNA ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA samples were depleted of rRNA with the NEBNext rRNA Depletion kit (NEB) prior to preparing cnRNA-seq libraries using the NEBNext Ultra (for Bap1fl/fl and PRC1CPM ESCs ...
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bioRxiv - Microbiology 2020Quote: ... The DNA was repaired using FFPE and end-repair kits (New England BioLabs) followed by ligation of barcodes with bead clean up using AMPure XP (Beckman Coulter ...
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bioRxiv - Genetics 2021Quote: ... Total RNA was extracted using the Monarch Total RNA Miniprep Kit (#T2010S, NEB), following the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2020Quote: HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB, Cat. No. E2050S) was used for in vitro transcription of COVID19 N RNA according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... 5’ RNA ligation was performed using 0.5 μl 5’ SR Adapater (NEB-kit) instead of the 5P RNA oligo.
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bioRxiv - Genomics 2020Quote: ... m6A-immunoprecipitaion was performed using the EpiMark N6-Methyladenosine Enrichment Kit (NEB E1610S), followed by library preparation using the SMARTer Stranded Total RNA-seq kit v2 (Takara ...
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bioRxiv - Cell Biology 2020Quote: ... generated using the Protoscript II First Strand cDNA Synthesis kit (NEB Cat# E6560S). cDNAs were inserted into pIRES-3xFlag-dsRed by restriction digestion and ligation ...
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bioRxiv - Microbiology 2020Quote: ... final quantification was performed using the NEBNext Library Quant Kit for Illumina (NEB) according to manufacture protocol ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were purified using the Monarch PCR and DNA Cleanup Kit (NEB) and sequenced using the Sanger method with M13F or M13R primers.
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bioRxiv - Microbiology 2020Quote: ... and NEBNext Library Quant Kit for Illumina (New England BioLabs, Ipswich, Massachusetts, USA). Sequencing was conducted using MiSeq V3 reagent kit with 250 bp reads ...
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bioRxiv - Microbiology 2021Quote: ... libraries were made using the NEB Ultra II RNA custom kit (NEB, E7530S) on an Agilent Bravo WS automation system ...
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bioRxiv - Pathology 2020Quote: ... and NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs), following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... PCR products were purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs). RT ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR products were converted to RNA using the T7 RNA Transcription Kit (NEB) and purified with RNEasy Kit (Qiagen ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA synthesis was performed using LunaScript™ RT SuperMix Kit (New England Biolabs) and in a standard thermocycler according to manufacturer instructions ...
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bioRxiv - Genetics 2020Quote: ... rnr1Y285F and rnr1Y285A using the Q5 site-directed mutagenesis kit (New England Biolabs) (Table S3) ...
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bioRxiv - Genetics 2020Quote: ... Libraries were prepared with the NEBNext Ultra II Library Preparation Kit (NEB #E7103). Samples were sequenced on a combination of Illumina’s NovaSeq 6000 and HiSeq 4000 at Novogene.
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bioRxiv - Genetics 2021Quote: ... library construction was performed using the NEBNext Ultra-II kit (New England Biolabs) following manufacturer recommendations ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and prepared using NEBNext Ultra II Directional RNA Library Prep Kit (NEB #E7765) and single indexing from the NEBNext Multiplex Oligos kit (NEB #E7555L ...
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bioRxiv - Physiology 2021Quote: ... Synthesis of cDNA was performed with the LunaScript RT SuperMix Kit (NEB, #E3010L). For quantitative real-time PCR (qPCR ...
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bioRxiv - Developmental Biology 2020Quote: ... cDNA was generated using Protoscript II First Strand Synthesis Kit (New England Biolabs). Lmo7a rescue constructs and lmo7a:sfGFP fusion constructs were generated by Gibson Assembly (Gibson et al ...
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bioRxiv - Microbiology 2021Quote: ... and re-cloned into the vector using the NEBuilder kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... were transcribed in vitro using an EnGen® sgRNA Synthesis Kit (NEB, E3322S). The generation and characterization of CRTD5 hiPSCs (hPSCreg ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids expressing mgrB variants were constructed using Q5 site-directed mutagenesis kit (NEB). All constructs were confirmed by DNA sequencing.
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bioRxiv - Immunology 2022Quote: ... cDNA libraries were prepared using NEBNext Ultra DNA library kits (New England BioLabs) for Illumina ...
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bioRxiv - Cancer Biology 2021Quote: ... using a Gibson Assembly® Cloning Kit (New England BioLabs; Cat. No. E2611). The library was produced in MegaX DH10B T1R Electrocomp Cells (Invitrogen ...