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Citations for New England Biolabs :
2251 - 2300 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... After electrophoresis and gel extraction by Monarch DNA Gel Extraction Kit (T1020S; NEB), the PCR product was transcribed using HiScribe T7 High Yield RNA Synthesis Kit (E2040S ...
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bioRxiv - Microbiology 2022Quote: ... the amplicons were purified with the Monarch® PCR & DNA Cleanup Kit (NEB). High-throughput sequencing was performed by Novogene using the Illumina NovaSeq PE250 system (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... and the remainder was cloned by using PCR Cloning Kit (New England Biolabs) and analyzed by Sanger sequencing.
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bioRxiv - Microbiology 2022Quote: ... and the sequences were introduced with the Q5 site-directed mutagenesis kit (NEB). Transfection of both the TATi cassette and the Cas9/guide RNA vector was performed as above ...
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bioRxiv - Microbiology 2022Quote: ... and DNase was removed by using the Monarch RNA Cleanup Kit (50μg; NEB). LunaScript RT SuperMix Kit (NEB ...
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bioRxiv - Microbiology 2022Quote: ... we used the Monarch HMW DNA Extraction Kit for Tissue (NEB, MA, USA) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... cell DNA was extracted using the Monarch genomic DNA purification kit (NEB #T3010L) or the NucleoSpin Tissue kit (Macherey-Nagel #740952-250 ...
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bioRxiv - Microbiology 2022Quote: ... Ribosomal RNA depletion was performed with the NEBNext Bacteria kit (NEB, Frankfurt, Germany). The libraries were prepared with the TruSeq kit (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... and the RNA was purified with Monarch® RNA clean-up kit (NEB). sgRNA aliquots were stored at −80°C.
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bioRxiv - Molecular Biology 2022Quote: ... Mutations were made with Q5® Site-Directed Mutagenesis Kit (New England Biolabs) using primer sets (Supplementary file 1) ...
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bioRxiv - Plant Biology 2022Quote: ... sequencing libraries were generated using Directional NEBNext Ultra RNA Library Prep Kit (NEB) (Novogene ...
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bioRxiv - Plant Biology 2022Quote: ... Point mutations were generated using a Q5 mutagenesis kit (New England Biolabs, USA). Plasmids were amplified in DH5α E ...
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bioRxiv - Molecular Biology 2023Quote: ... and NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs, #E7765) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... purified PCR products were cloned using NEB PCR Cloning Kit (New England BioLabs). Clones were Sanger sequenced at the University of Minnesota Genomics Center.
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bioRxiv - Molecular Biology 2023Quote: ... Human FBP1 mutants were constructed by Q5 Site-Directed Mutagenesis Kit (NEB, E0554S) in the PCDH-V5-FBP1 vectors ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing libraries were made using the Small RNA library preparation kit NEBNext (NEB). Sequencing was performed on the Illumina HiSeq 2500 using the Rapid Run mode.
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bioRxiv - Microbiology 2023Quote: ... and tasA_downstream_rv and the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs).
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bioRxiv - Microbiology 2023Quote: ... and was further prepared for sequencing using NEBNext Ultra FS II kit (NEB) with the miniaturized protocol (22) ...
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bioRxiv - Microbiology 2022Quote: All enzymes and DNA preparation kits used were from New England Biolabs (NEB), and all PCR products were amplified using Phusion polymerase ...
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bioRxiv - Bioengineering 2022Quote: ... and assembled into the pTlpA39-Wasabi vector (Addgene #86116)[46] using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, GmbH). The mCherry gene was inserted downstream of the PtlpA promoter by replacing the mWasabi reporter gene ...
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bioRxiv - Biochemistry 2023Quote: ... The points mutations were inserted using the Q5 mutagenesis kit (New England Biolabs) and specific primers ...
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bioRxiv - Biochemistry 2023Quote: ... was produced by QuikChange site-directed mutagenesis (NEB, Phusion High-Fidelity PCR Kit) followed by KLD (Kinase ...
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bioRxiv - Biochemistry 2023Quote: ... was generated using site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, NEB E0554S) of the SARS-CoV-2 Positive Control (N gene ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was first circularized by a T4 RNA Ligase 1 Kit (M0204. NEB) and then purified by TRIzol reagent followed by isopropanol precipitation ...
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bioRxiv - Cancer Biology 2023Quote: ... After quantification by qPCR using the NEBnext library quant kit for Illumina (NEB), pooled libraries were sequenced on an Illumina HiSeq 2500 platform using a 50 bp single read on a high output standard v4 flow cell with a 10–20% PhiX spike-in ...
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bioRxiv - Cancer Biology 2022Quote: ... DHFR control plasmid from PURExpress In Vitro Protein Synthesis Kit (New England Biolabs) was used as backbone for subsequent ligations ...
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bioRxiv - Genomics 2022Quote: ... The resulting fragments were purified using the Monarch PCR & DNA Cleanup Kit (NEB). Libraries were constructed using Illumina Nextera Dual Indexing ...
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bioRxiv - Genomics 2022Quote: ... the original ORI was excised using a Site Directed Mutagenesis kit (NEB E0554S). The vector was transformed (NEB C3040H ...
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bioRxiv - Molecular Biology 2023Quote: ... and mutations were introduced using the Q5 Site-Directed Mutagenesis Kit (NEB # E0554S) or were synthesized by Genscript ...
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bioRxiv - Molecular Biology 2023Quote: ... dsRNA was generated by in vitro transcription using the HiScribe T7 kit (NEB), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Secondary PCRs were purified with the Monarch PCR and DNA Cleanup Kit (NEB), ligated into the pCR2.1-TOPO TA cloning vector (Invitrogen) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... double strand cDNA was synthesized using NEBNext mRNA second strand synthesis kit (NEB), double strand DNA was washed with AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Plant Biology 2023Quote: ... Library was prepared using the NEBNext Ultra Directional RNA Library Prep Kit (NEB) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... rRNA depletion was conducted using NEBNext rRNA Depletion Kit (Bacteria, New England Biolabs) according to the manufacturer’s recommended protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA was purified by Monarch® RNA Cleanup Kit (New England Biolabs). Binding of ribosomes to leaderless mRNA was tested by assembling 10-500 fmol of 70S ribosomes with 10 fmol of NAD/ ppp-leaderless mRNA in translation buffer (10 mM Tris-HCl pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... and the NEBNext Ultra II directional RNA library Prep kit (NEB, Cat#E7785S) for Illumina were used for index library prep following NEB #E7760S/L ...
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bioRxiv - Bioengineering 2023Quote: The Luna Universal Probe One-Step RT-qPCR kit (New England Biolabs, USA) was used ...
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bioRxiv - Bioengineering 2023Quote: ... and the linearized plasmid was re-ligated with a Quick Ligase Kit (NEB) following manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The linearized DNAs were purified using Monarch DNA cleanup kit (NEB, United States) and the capped RNA transcripts were synthesized using the SP6 mMessage mMachine kit (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... Sequencing libraries were prepared using NEB Next Ultra II kit (New England Biolabs). The resulting DNA quantity and size distribution was assessed by Qubit instrument (Invitrogen ...
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bioRxiv - Plant Biology 2024Quote: ... The NebNext Ultra II RNA library prep kit (New England Biolabs, MA, USA) was used to synthesize RNA libraries ...
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bioRxiv - Microbiology 2024Quote: ... The amplified PCR products were ligated using a Gibson assembly kit (NEB, USA). The resulting plasmid pLZ asnA R100K was electroporated into strain S119 ΔasnA for expression of mutated asnA.
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bioRxiv - Cell Biology 2024Quote: ... Total RNA was isolated using NEB Monarch RNA prep kit (New England BioLabs). The RNA concentration and RIN (RNA integrity number ...
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bioRxiv - Genomics 2024Quote: ... and used for Illumina library using the NEBNext ultra II kit (NEB E7600). Briefly ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR products were purified using a Monarch PCR Cleanup Kit (New England Biolabs) and prepared for nanopore long read sequencing using the Ligation Sequencing Kit (Oxford Nanopore Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... PCR products were purified using Monarch DNA Gel Extraction Kit (New England Biolabs). Both the vector and the complete insert were digested by BsmBI (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... before purifying again using an NEB RNA clean and concentrate kit (NEB #T2030).
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bioRxiv - Microbiology 2024Quote: ... NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs) was used for the following RNA-seq library preparation according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... PCR products were purified using a Monarch PCR & DNA Cleanup Kit (NEB, T1030S) following the Manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... The DNA was then purified using the Monarch PCR & DNA Cleanup Kit (NEB). The first-dimension electrophoresis was run in 0.7% agarose in 1xTBE buffer at 0.7 V/cm for 18h ...