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Citations for New England Biolabs :
1851 - 1900 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Plasmids were recovered using Monarch Plasmid Miniprep Kit (New England Biolabs #T1010S) or E.Z.N.A ...
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bioRxiv - Microbiology 2024Quote: ... Digested products were cleaned using Monarch® PCR & DNA Cleanup Kit (NEB) and incubated with T4 DNA ligase (NEB ...
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bioRxiv - Microbiology 2024Quote: ... and cleaned with the Monarch RNA Cleanup Kit (NEB, Ipswich, MA, USA). Ribosomal 23S and 16S samples were prepared by amplifying the appropriate sequences from genomic E ...
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bioRxiv - Microbiology 2024Quote: Total RNA samples were treated with a NEBNext rRNA depletion kit (NEB) to remove rRNA ...
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bioRxiv - Biochemistry 2024Quote: ... Transcription was performed using T7 HiScribe RNA synthesis kit (New England Biolabs). Generated RNA was subjected to DNase treatment (TURBODNase ...
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bioRxiv - Cancer Biology 2024Quote: ... using the NEBNext® Ultra TM II DNA Library Prep Kit (NEB E7645S/L ...
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bioRxiv - Molecular Biology 2024Quote: ... and purified with the Monarch Total RNA Miniprep Kit (New England Biolabs). Alkylated RNA was purified with the Monarch RNA Cleanup Kit (New England Biolabs) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and the transcript was purified with Monarch RNA Cleanup Kit (NEB T2040L).
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bioRxiv - Plant Biology 2024Quote: ... The NEB Next Ultra RNA Library Prep Kit for Illumina (NEB, USA) was utilized to generate sequencing libraries [15] ...
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bioRxiv - Synthetic Biology 2024Quote: ... Samples were purified using the Monarch RNA Cleanup Kit (New England Biolabs) and transferred to fresh ...
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bioRxiv - Neuroscience 2024Quote: ... and converted into cDNA (LunaScript RT Supermix Kit New England Biolabs E3010S). Approximately 500 ng of cDNA was used per reaction (Luna Universal qPCR Master Mix New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... using the NEBuilder® HiFi DNA Assembly Cloning Kit (New England Biolabs). The four-fragment Gibson [39] Assembly reactions included an 89 bp single-stranded oligo designed to bridge the N-terminal end of the Cas9 sequence with the T7 promoter end of the pET backbone ...
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bioRxiv - Microbiology 2024Quote: ... 1 µg RNA was reverse transcribed using LunaScript RT SuperMix Kit (NEB). qPCR was performed using synthesised cDNA ...
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bioRxiv - Genomics 2024Quote: ... libraries prepared (NEBNext Ultra II DNA Library prep Kit, New England Biolabs), and index tags applied (Sanger 168 tag set) ...
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bioRxiv - Biochemistry 2024Quote: ... and New England Biolabs PURExpress In Vitro Protein Synthesis Kit (NEB #E6800). All E ...
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bioRxiv - Cancer Biology 2024Quote: ... ribosomal RNA depleted using NEBNext rRNA Depletion Kit (NEB, Human/Mouse/Rat) and strand-specific library preparation used NEBNext Ultra II mRNA kit (NEB ...
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bioRxiv - Microbiology 2024Quote: ... mRNAs were purified using the Monarch RNA Cleanup Kit (New England Biolabs).
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bioRxiv - Genomics 2024Quote: DNA was processed using the NEBNext Enzymatic Methyl-seq Kit (NEB, USA) as per the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... PCR products were purified using a Monarch PCR & DNA cleanup kit (NEB), and products were electrophoresed on an agarose TAE gel to confirm the product was of the correct size ...
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bioRxiv - Biochemistry 2024Quote: ... The samples were then purified with the Monarch RNA cleanup kit (NEB), and the RNA concentration was determined by absorbance at 260 nm on a Nanodrop spectrophotometer (Thermo Scientific) ...
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bioRxiv - Genomics 2024Quote: ... the NEBNext Ultra II DNA Library Prep kit (New England BioLabs, E7645) was used for End Prep and Adapter Ligation ...
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bioRxiv - Genomics 2024Quote: ... and quantified with the NEBNext Library Quant Kit for Illumina (E7630L, NEB). Libraries were then pooled and sequenced in 150-cycle single-end format on an Illumina NextSeq 550.
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bioRxiv - Bioengineering 2024Quote: Plasmids were constructed using the NEBuilder® HiFi DNA Assembly kit (NEB), and PCR reactions were performed with NEB Q5 High-Fidelity Polymerase ...
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bioRxiv - Biochemistry 2024Quote: ... was transcribed in vitro using the HiScribe T7 Kit with CleanCap (NEB), replacing partial GTP with m7G(5’)ppp(5’)A RNA cap structure analogous to the 5’ capped mRNA ...
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bioRxiv - Biochemistry 2024Quote: ... Mutations were introduced using a Q5 Site-Directed Mutagenesis Kit (NEB #E0554S). Plasmids were amplified in DH5α cells and purified using QIAprep Spin Miniprep Kits (Qiagen) ...
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bioRxiv - Cancer Biology 2024Quote: ... Adenylated 3’ adapter was prepared using the 5’ DNA adenylation kit (NEB) and ligated using T4 RNA ligase 2 ...
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bioRxiv - Systems Biology 2024Quote: ... Chromosomal DNA was extracted using Monarch genomic DNA purification kit (NEB #T3010S). The barcode region was amplified with primers prDS292-prDS293 which include Illumina sequencing adaptors (Phusion HiFi mastermix standard protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... Libraries were quantified with the NEBnext library quant kit for Illumina (NEB) and then sequenced for approximately 40 – 60M 1x 75 nt single reads on a NextSeq500 (Illumina ...
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bioRxiv - Synthetic Biology 2024Quote: ... RNA products were purified using Monarch RNA Cleanup Kit (NEB, cat T2040S) and stored in −80°C.
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bioRxiv - Cancer Biology 2024Quote: ... in conjunction with Poly(A) mRNA Magnetic Isolation Module kit (NEB, E7490L) was then used to prepare libraries according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... and R164G were made using the Q5 Site-Directed Mutagenesis Kit (NEB). Primers were designed using NEBaseChanger™ ...
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bioRxiv - Developmental Biology 2024Quote: ... we used the NEBNext Ultra II DNA Library Prep kit (NEB, E7103S) with NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... Adenylated 3’ adapter was prepared using the 5’ DNA adenylation kit (NEB) and ligated using T4 RNA ligase 2 ...
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bioRxiv - Cancer Biology 2024Quote: ... and strand-specific library preparation used NEBNext Ultra II mRNA kit (NEB) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... or homologous recombination based NEBuilder® HiFi DNA Assembly kit (NEB, MA). Some p62 structural mutant expression plasmids were constructed specifically for the present study ...
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bioRxiv - Cancer Biology 2024Quote: ... or an NEBNext® Library Quant Kit for Illumina® (NEB E7630). The final libraries were sequenced using an Illumina MiSeq device and MiSeq Reagent Kit v2 (Illumina MS-102-2002).
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bioRxiv - Bioengineering 2024Quote: ... and PCR products were extracted using Monarch DNA Gel Extraction Kit (NEB). The gel-extracted library was quantified by Nanodrop (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... and ligated together with NEBuilder HiFi DNA Assembly Kit (New England Biolabs). Escherichia coli DH5α competent cells were transformed with the ligation mixtures and the cells containing assembled plasmid were selected on an agar medium supplemented with 100 mg/l ampicillin ...
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bioRxiv - Cell Biology 2024Quote: ... NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, cat. no. E7370) was used to do the library according to the manufacturer protocol with the following index set NEBNext Multiplex Oligos for Illumina (Dual-Index Primers Set 1 ...
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bioRxiv - Bioengineering 2024Quote: ... Full-length gene band was gel-extracted (Monarch Gel Extraction Kit, NEB). USER enzyme digestion ...
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Effective targeting of CD19 positive primary B-ALL cells using CAR-NK cells generated with mRNA-LNPsbioRxiv - Cancer Biology 2024Quote: ... the mRNA was purified using the Monarch RNA Cleanup Kit (NEB #T2030) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... after ribosomal RNA (rRNA) depletion (NEBNext rRNA Depletion Kit; New England Biolabs). Paired-end (2 × 36 bases ...
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bioRxiv - Genetics 2024Quote: All plasmids are made with Gibson assembly (NEB HIFI DNA assembly kit) according to manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... the samples were purified using a Monarch Nuclei Acid Purification Kit (NEB) as per manufacturer’s instructions and eluted in 20 μL of nuclease free water ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Lysis was performed with Monarch® Genomic DNA Purification Kit (NEB, #T3010S) following the manufacturer’s protocol for Tissue Lysis with an overnight incubation at 56 °C ...
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bioRxiv - Cell Biology 2024Quote: ... Using the NEB Next Ultra RNA Library Prep Kit for Illumina (NEB), which selects for poly(A ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was converted to dsDNA (Second Strand synthesis kit, NEB, Cat#: E611L) and linearly amplified by in vitro transcription (HiScribe T7 High Yield RNA Synthesis Kit ...
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bioRxiv - Microbiology 2020Quote: ... total RNA was collected and prepared using Monarch Total RNA Miniprep Kits (NEB). For SARS-CoV-2 experiments ...
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bioRxiv - Plant Biology 2020Quote: ... followed by subcloning into pEAQ-HT vector using the Gibson assembly kit (NEB). Linearization of pEAQ-HT vector was performed by XhoI and AgeI restriction enzyme double digestion ...
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bioRxiv - Microbiology 2020Quote: ... chloramphenicol or apramycin resistance cassette by using the Gibson isothermal assembly kit (NEB) followed by PCR amplification using the primers from the extremities ...